DE69637086D1 - Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül - Google Patents

Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül

Info

Publication number
DE69637086D1
DE69637086D1 DE69637086T DE69637086T DE69637086D1 DE 69637086 D1 DE69637086 D1 DE 69637086D1 DE 69637086 T DE69637086 T DE 69637086T DE 69637086 T DE69637086 T DE 69637086T DE 69637086 D1 DE69637086 D1 DE 69637086D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid molecule
repeat sequence
multiple copies
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69637086T
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English (en)
Other versions
DE69637086T2 (de
Inventor
Peter M Lansdorp
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Individual
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Individual
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Filing date
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Application filed by Individual filed Critical Individual
Publication of DE69637086D1 publication Critical patent/DE69637086D1/de
Application granted granted Critical
Publication of DE69637086T2 publication Critical patent/DE69637086T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69637086T 1995-10-12 1996-10-10 Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül Expired - Lifetime DE69637086T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US559095P 1995-10-12 1995-10-12
US5590P 1995-10-12
US761695P 1995-11-28 1995-11-28
US7616P 1995-11-28
PCT/CA1996/000676 WO1997014026A2 (en) 1995-10-12 1996-10-10 Method for detecting multiple copies of a repeat sequence in a nucleic acid molecule

Publications (2)

Publication Number Publication Date
DE69637086D1 true DE69637086D1 (de) 2007-06-28
DE69637086T2 DE69637086T2 (de) 2008-01-10

Family

ID=26674516

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69637086T Expired - Lifetime DE69637086T2 (de) 1995-10-12 1996-10-10 Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül

Country Status (5)

Country Link
US (2) US6514693B1 (de)
EP (1) EP0870055B1 (de)
AT (1) ATE362546T1 (de)
DE (1) DE69637086T2 (de)
WO (1) WO1997014026A2 (de)

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US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
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EP0878552A1 (de) * 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molekularer Nachweis von chromosomalen Veränderungen
JP2001501975A (ja) * 1997-05-28 2001-02-13 ペーター・エー・ニールセン 亢進した細胞取り込みを有する結合ペプチド核酸
US6900012B1 (en) 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
ATE478090T1 (de) 1997-10-27 2010-09-15 Boston Probes Inc SICH AUF ßPNA MOLECULAR BEACONSß BEZIEHENDE VERFAHREN, TESTSÄTZE UND ZUSAMMENSETZUNGEN
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
AU3516899A (en) * 1998-05-04 1999-11-23 Dako A/S Method and probes for the detection of chromosome aberrations
JP2003524754A (ja) * 1998-05-16 2003-08-19 ピーイー コーポレイション （エヌワイ） Ｄｎａのポリメラーゼ連鎖反応をモニタする装置
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US6664045B1 (en) 1998-06-18 2003-12-16 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms
US20030144225A1 (en) * 2001-12-28 2003-07-31 Karras James G. Antisense modulation of macrophage inflammatory protein 3-alpha expression
US7981599B1 (en) 1998-07-31 2011-07-19 Boston Probes, Inc. Non-nucleic acid probes, probe sets, methods and kits pertaining to the detection of individual human chromosomes X, Y, 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 16, 17, 18 and 20 as 13/21 as a pair
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
US7060432B1 (en) 1999-06-15 2006-06-13 Applera Corporation Methods for the detection, identification, and/or enumeration of yeast, particularly in wine
US6541202B1 (en) 1999-10-13 2003-04-01 Research & Development Institute, Inc. Telomerase reverse transcriptase (TERT) genes from Candida albicans
EP1432826B1 (de) 2001-09-24 2012-12-12 Life Technologies Corporation Verfahren, reagentiensätze und zusammensetzungen zur unterdrückung der bindung von detektierbaren sonden an zufällig verteillten repeat sequenzen in genomischen nukleinsäuren
JP5686493B2 (ja) 2003-01-24 2015-03-18 ユニバーシティ・オブ・ユタＵｎｉｖｅｒｓｉｔｙ Ｏｆ Ｕｔａｈ テロメアの長さを決定することによって死亡の危険性を予測する方法
MXPA06009225A (es) 2004-02-23 2007-03-08 Chromatin Inc Plantas modificadas con mini-cromosomas.
EP1745156A2 (de) 2004-05-04 2007-01-24 Dako Denmark A/S Verfahren für den nachweis von chromosomenaberrationen
WO2006026714A2 (en) * 2004-08-31 2006-03-09 Board Of Regents, The University Of Texas System Diagnosis and prognosis of cancer based on telomere length as measured on cytological specimens
US8407013B2 (en) 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US7734424B1 (en) 2005-06-07 2010-06-08 Rogan Peter K Ab initio generation of single copy genomic probes
AU2006287553A1 (en) 2005-09-08 2007-03-15 Chromatin, Inc. Plants modified with mini-chromosomes
US20070238104A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Competitive oligonucleotides
WO2008112972A2 (en) 2007-03-15 2008-09-18 Chromatin, Inc. Centromere sequences and minichromosomes
US8093063B2 (en) * 2007-11-29 2012-01-10 Quest Diagnostics Investments Incorporated Assay for detecting genetic abnormalities in genomic nucleic acids
KR101072900B1 (ko) * 2007-12-04 2011-10-17 주식회사 파나진 고정화된 펩티드핵산 프로브를 사용한 표적핵산의 선택적 표지 및 검출 방법
US8084203B2 (en) * 2008-05-12 2011-12-27 Fundacion Centro Nacional De Investigaciones Oncologicas Carlos Iii Methods for the determination of telomere length in a semi-automatic manner of every single cell in a immobilized cell population
US20090298709A1 (en) * 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
NO3029154T3 (de) 2008-10-17 2018-03-03
CN107083444B (zh) 2008-12-22 2023-03-14 犹他大学研究基金会 单色多重定量pcr
EP2456894A4 (de) 2009-07-23 2013-09-25 Chromatin Inc Sorghum-zentromer-sequenzen und -minichromosomen
WO2013074601A1 (en) 2011-11-14 2013-05-23 Carnegie Mellon University Center For Technology Transfer & Enterprise Gamma-pna miniprobes for fluorescent labeling
US20130183666A1 (en) * 2012-01-18 2013-07-18 Marc N. Feiglin Partial genotyping by differential hybridization
US9200327B2 (en) 2012-11-30 2015-12-01 Geron Corporation Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors
US10316366B2 (en) 2013-05-22 2019-06-11 Telomere Diagnostics, Inc. Measures of short telomere abundance
US9476884B2 (en) 2013-10-04 2016-10-25 University Of Massachusetts Hybridization- independent labeling of repetitive DNA sequence in human chromosomes
WO2015179557A1 (en) * 2014-05-20 2015-11-26 Sens Foundation, Inc. High throughput telomeric circle assay
CN107208145A (zh) 2014-12-30 2017-09-26 端粒诊断公司 多重定量pcr
US20230183780A1 (en) 2016-07-25 2023-06-15 InVivo BioTech Services GmbH Dna probes for in situ hybridization on chromosomes
CN108004299B (zh) * 2017-10-23 2021-07-06 济南海湾生物工程有限公司 一种使用基因组dna测定端粒长度的荧光定量原位杂交(q-fish)方法
FR3085964B1 (fr) 2018-09-18 2023-09-01 Cell Env Procede a haut debit pour detecter des aberrations chromosomiques et/ou des aberrations de telomeres

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DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5641625A (en) 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
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US5830644A (en) * 1992-05-13 1998-11-03 Geron Corporation Method for screening for agents which increase telomerase activity in a cell
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DE4216949C2 (de) 1992-05-22 1997-07-24 Christoph Prof Dr Dr Cremer Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben
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AU2522095A (en) 1994-05-19 1995-12-18 Dako A/S Pna probes for detection of neisseria gonorrhoeae and chlamydia trachomatis
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ATE362546T1 (de) * 1995-10-12 2007-06-15 Lansdorp Peter M Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül

Also Published As

Publication number Publication date
DE69637086T2 (de) 2008-01-10
WO1997014026A2 (en) 1997-04-17
ATE362546T1 (de) 2007-06-15
EP0870055A2 (de) 1998-10-14
WO1997014026A3 (en) 1997-07-24
US20030022204A1 (en) 2003-01-30
US6514693B1 (en) 2003-02-04
EP0870055B1 (de) 2007-05-16

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