DE69623494D1 - Elektrochemische erkennung von nukleinsäurehybridation - Google Patents

Elektrochemische erkennung von nukleinsäurehybridation

Info

Publication number
DE69623494D1
DE69623494D1 DE69623494T DE69623494T DE69623494D1 DE 69623494 D1 DE69623494 D1 DE 69623494D1 DE 69623494 T DE69623494 T DE 69623494T DE 69623494 T DE69623494 T DE 69623494T DE 69623494 D1 DE69623494 D1 DE 69623494D1
Authority
DE
Germany
Prior art keywords
nucleic acid
electrochemical detection
substrate
microelectronic device
hybridation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69623494T
Other languages
English (en)
Other versions
DE69623494T2 (de
Inventor
Holden H Thorp
Dean H Johnston
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of North Carolina at Chapel Hill
University of North Carolina System
Original Assignee
University of North Carolina at Chapel Hill
University of North Carolina System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/667,338 external-priority patent/US5871918A/en
Application filed by University of North Carolina at Chapel Hill, University of North Carolina System filed Critical University of North Carolina at Chapel Hill
Publication of DE69623494D1 publication Critical patent/DE69623494D1/de
Application granted granted Critical
Publication of DE69623494T2 publication Critical patent/DE69623494T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nanotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Battery Electrode And Active Subsutance (AREA)
  • Battery Mounting, Suspending (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69623494T 1995-06-27 1996-06-24 Elektrochemische erkennung von nukleinsäurehybridation Expired - Fee Related DE69623494T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US49581795A 1995-06-27 1995-06-27
US1626596P 1996-04-19 1996-04-19
US08/667,338 US5871918A (en) 1996-06-20 1996-06-20 Electrochemical detection of nucleic acid hybridization
PCT/US1996/010702 WO1997001646A2 (en) 1995-06-27 1996-06-24 Electrochemical detection of nucleic acid hybridization

Publications (2)

Publication Number Publication Date
DE69623494D1 true DE69623494D1 (de) 2002-10-10
DE69623494T2 DE69623494T2 (de) 2003-04-17

Family

ID=27360533

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69623494T Expired - Fee Related DE69623494T2 (de) 1995-06-27 1996-06-24 Elektrochemische erkennung von nukleinsäurehybridation

Country Status (12)

Country Link
EP (2) EP1193315A1 (de)
JP (1) JP2000501601A (de)
CN (1) CN1192249A (de)
AT (1) ATE223498T1 (de)
AU (1) AU724600B2 (de)
CA (1) CA2225935A1 (de)
DE (1) DE69623494T2 (de)
DK (1) DK0871773T3 (de)
HK (1) HK1047453A1 (de)
NO (2) NO976057L (de)
NZ (1) NZ311955A (de)
WO (1) WO1997001646A2 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5952172A (en) 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US6180346B1 (en) * 1995-06-27 2001-01-30 The Universtiy Of North Carolina At Chapel Hill Electropolymerizable film, and method of making and use thereof
US6346387B1 (en) * 1995-06-27 2002-02-12 Xanthon, Inc. Detection of binding reactions using labels detected by mediated catalytic electrochemistry
US6444423B1 (en) 1996-06-07 2002-09-03 Molecular Dynamics, Inc. Nucleosides comprising polydentate ligands
CA2257534A1 (en) * 1996-06-07 1997-12-11 California Institute Of Technology Nucleic acid mediated electron transfer
US7014992B1 (en) * 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US7045285B1 (en) 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
US7160678B1 (en) 1996-11-05 2007-01-09 Clinical Micro Sensors, Inc. Compositions for the electronic detection of analytes utilizing monolayers
US6391558B1 (en) 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences
US6013459A (en) * 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
AU2559599A (en) * 1998-01-20 1999-08-02 Bruce Parkinson Detection of genetic information
US6686150B1 (en) 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6290839B1 (en) 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6740518B1 (en) 1998-09-17 2004-05-25 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
AU1241000A (en) 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US6833267B1 (en) 1998-12-30 2004-12-21 Clinical Micro Sensors, Inc. Tissue collection devices containing biosensors
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
CA2380258C (en) 1999-07-26 2008-07-15 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US7041445B2 (en) * 1999-11-15 2006-05-09 Clontech Laboratories, Inc. Long oligonucleotide arrays
WO2001065246A1 (de) * 2000-03-01 2001-09-07 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Quantifizierung von in einer flüssigkeit enthaltenen zielmolekülen
US7455975B2 (en) 2000-04-14 2008-11-25 Esa Biosciences, Inc. Electrochemical detection of nucleic acid sequences
US6602400B1 (en) 2000-06-15 2003-08-05 Motorola, Inc. Method for enhanced bio-conjugation events
US20020182606A1 (en) * 2001-06-04 2002-12-05 Xanthon, Inc. Detection of single nucleotide polymorphisms
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
EP1694867B1 (de) * 2003-12-15 2009-10-28 Geneohm Sciences, Inc. Kohlenstoffelektrodenoberfläche zur bindung von dna- und proteinmolekülen daran
JP5320077B2 (ja) * 2006-03-03 2013-10-23 ユニベルシテ パリ ディドロ−パリ ７ 標的核酸配列の電気化学的検出方法
KR101099517B1 (ko) * 2009-06-12 2011-12-27 연세대학교 산학협력단 엡타머의 구아닌 염기로부터 전자전달에 의한 분자 또는 이온 검출방법
CN103760202B (zh) * 2014-01-28 2016-03-09 东北师范大学 一种使用制备的电化学传感器对dna碱基同时检测的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0149339B1 (de) * 1983-12-16 1989-08-23 MediSense, Inc. Test für Nukleinsäuren
WO1991015768A1 (en) * 1990-04-06 1991-10-17 Syngene, Inc. Process and composition for performing dna assays
DE69125441T2 (de) * 1990-09-28 1997-11-06 Toshiba Kawasaki Kk Verfahren zum Gennachweis
US5272056A (en) * 1991-01-03 1993-12-21 The Research Foundation Of State University Of New York Modification of DNA and oligonucleotides using metal complexes of polyaza ligands
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
GB9206671D0 (en) * 1992-03-27 1992-05-13 Cranfield Biotech Ltd Electrochemical detection of dna hybridisation
FR2703359B1 (fr) * 1993-03-31 1995-06-23 Cis Bio Int Copolymère nucléotide(s)/polymère conducteur électronique ; son procédé de préparation et son utilisation .
WO1995000530A1 (en) * 1993-06-25 1995-01-05 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids

Also Published As

Publication number Publication date
CN1192249A (zh) 1998-09-02
EP1193315A1 (de) 2002-04-03
CA2225935A1 (en) 1997-01-16
NZ311955A (en) 1999-04-29
NO976057D0 (no) 1997-12-23
MX9800050A (es) 1998-08-30
DK0871773T3 (da) 2002-12-09
HK1047453A1 (zh) 2003-09-16
NO20045309L (no) 1998-02-24
AU6337696A (en) 1997-01-30
WO1997001646A2 (en) 1997-01-16
WO1997001646A3 (en) 1997-02-27
AU724600B2 (en) 2000-09-28
EP0871773A2 (de) 1998-10-21
ATE223498T1 (de) 2002-09-15
EP0871773B1 (de) 2002-09-04
NO976057L (no) 1998-02-24
DE69623494T2 (de) 2003-04-17
JP2000501601A (ja) 2000-02-15

Similar Documents

Publication Publication Date Title
DE69623494D1 (de) Elektrochemische erkennung von nukleinsäurehybridation
CA2311165A1 (en) Improvements in or relating to electrochemical assays
AU3780789A (en) Method and device employing photoresponsive semiconductor electrode for determining analyte
WO2001023885A8 (en) Test device
WO2003023360A3 (en) Methods and apparatus for conducting multiple measurements on a sample
WO2003001889A3 (en) Assay plates reader systems and methods for luminescence test measurements
DK0868761T3 (da) Elektrodesubstrat til brændstofcelle
ES2177586T3 (es) Un procedimiento electrobioquimico, sistema y electrodos para determinar un miembro de un par de reconocimiento.
DK0728207T3 (da) Telomeraseaktivitetsassays
CA2329564A1 (en) Cell for electrochemical analysis of a sample and method
WO1998035232A3 (en) Electrochemical detection of specific binding
CA2146381A1 (en) Method for Quantifying Specific Compound
DE69528859D1 (de) Zellpotentialmessvorrichtung mit mehreren Mikroelektroden
NZ306051A (en) Testing using electrochemiluminescence
EP0226470A3 (de) Materialien und Verfahren zum mikrochemischen Testen
CA2300406A1 (en) Method and apparatus for automatic analysis
AU8112898A (en) Method for identifying and/or analysing biological substances, present in a conductive liquid, device and affinity sensor used for implementing said method
CA2499049A1 (en) Method of making sensor electrodes
ATE198427T1 (de) Elektrochemischer festphasensynthese von polymere
ATE466280T1 (de) Versuchsanordnung zum nachweis von bindungspartnern
EP0251915A3 (de) Enzymsensor
CA2331688A1 (en) Detection of a target in a sample
WO2000072019A3 (en) Liposome-enhanced test device and method
GB2322707A (en) Electrochemical test device and related methods
CA2266978A1 (en) Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and use for cellular assays in drug discovery

Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee