DE69609184T2 - Verfahren zur synthese von oligonukleotiden - Google Patents

Verfahren zur synthese von oligonukleotiden

Info

Publication number
DE69609184T2
DE69609184T2 DE69609184T DE69609184T DE69609184T2 DE 69609184 T2 DE69609184 T2 DE 69609184T2 DE 69609184 T DE69609184 T DE 69609184T DE 69609184 T DE69609184 T DE 69609184T DE 69609184 T2 DE69609184 T2 DE 69609184T2
Authority
DE
Germany
Prior art keywords
segments
solution
alcohol group
linkage
residual
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69609184T
Other languages
English (en)
Other versions
DE69609184D1 (de
Inventor
Alan Livingston
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
La Jolla Pharmaceutical Co
Original Assignee
La Jolla Pharmaceutical Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by La Jolla Pharmaceutical Co filed Critical La Jolla Pharmaceutical Co
Publication of DE69609184D1 publication Critical patent/DE69609184D1/de
Application granted granted Critical
Publication of DE69609184T2 publication Critical patent/DE69609184T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
DE69609184T 1995-06-07 1996-05-16 Verfahren zur synthese von oligonukleotiden Expired - Fee Related DE69609184T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/474,999 US5856464A (en) 1995-06-07 1995-06-07 Selective capping solution phase oligonucleotide synthesis
PCT/US1996/007100 WO1996040708A2 (en) 1995-06-07 1996-05-16 Improved methods for oligonucleotide synthesis

Publications (2)

Publication Number Publication Date
DE69609184D1 DE69609184D1 (de) 2000-08-10
DE69609184T2 true DE69609184T2 (de) 2001-02-15

Family

ID=23885823

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69609184T Expired - Fee Related DE69609184T2 (de) 1995-06-07 1996-05-16 Verfahren zur synthese von oligonukleotiden

Country Status (10)

Country Link
US (2) US5856464A (de)
EP (1) EP0830366B1 (de)
JP (1) JPH11510790A (de)
KR (1) KR19990022711A (de)
CN (1) CN1190973A (de)
AT (1) ATE194355T1 (de)
AU (1) AU694359B2 (de)
CA (1) CA2222440A1 (de)
DE (1) DE69609184T2 (de)
WO (1) WO1996040708A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856464A (en) * 1995-06-07 1999-01-05 Lajolla Pharmaceutical Company Selective capping solution phase oligonucleotide synthesis
CN1307421C (zh) 1997-08-08 2007-03-28 塞米得肿瘤学美国公司 克服生物及化学治疗抗性的方法与组合物
US7462605B2 (en) * 1998-01-23 2008-12-09 Celmed Oncology (Usa), Inc. Phosphoramidate compounds and methods of use
DE69900841T2 (de) * 1998-01-23 2002-10-02 Newbiotics Inc Durch enzymkatalyse erhaltene therapeutische substanzen.
US6096899A (en) * 1998-04-14 2000-08-01 The Regents Of The University Of Michigan Cylic imidazole compounds having relatively low hydrogen content and relatively high nitrogen content and polymers formed therefrom
CN1391486A (zh) * 1999-07-22 2003-01-15 新生物公司 治疗对治疗有抗性的肿瘤的方法
US6683061B1 (en) 1999-07-22 2004-01-27 Newbiotics, Inc. Enzyme catalyzed therapeutic activation
US6451998B1 (en) 1999-10-18 2002-09-17 Agilent Technologies, Inc. Capping and de-capping during oligonucleotide synthesis
WO2001041813A2 (en) * 1999-11-28 2001-06-14 La Jolla Pharmaceutical Company Methods of treating lupus based on antibody affinity and screening methods and compositions for use thereof
EP1176151B1 (de) * 2000-07-28 2014-08-20 Agilent Technologies, Inc. Synthese von Polynukleotiden unter Gebrauch von kombinierter Oxidations- und Entschützungschemie
CN1471537A (zh) * 2000-09-07 2004-01-28 ƽ 用于低聚核苷酸合成的合成子
CA2441350A1 (en) 2001-01-19 2002-07-25 Newbiotics, Inc. Methods to treat autoimmune and inflammatory conditions
US20030114405A1 (en) * 2001-08-13 2003-06-19 Linnik Matthew D. Methods of treating systemic lupus erythematosus in individuals having significantly impaired renal function
US7105135B2 (en) * 2001-10-16 2006-09-12 Lockheed Martin Corporation System and method for large scale detection of hazardous materials in the mail or in other objects
US6696298B2 (en) 2001-12-07 2004-02-24 Biosearch Technologies, Inc. Multi-channel reagent dispensing apparatus
AU2003303524A1 (en) * 2002-12-27 2004-07-29 La Jolla Pharmaceutical Company Methods of improving health-related quality of life in individuals with systemic lupus erythematosus
WO2004089422A2 (en) * 2003-03-30 2004-10-21 La Jolla Pharmaceutical Co. Methods of treating and monitoring systemic lupus erythematosus in individuals
US20070136064A1 (en) * 2003-04-16 2007-06-14 Carroll David W Mobile personal computer with movement sensor
WO2008045535A2 (en) * 2006-10-12 2008-04-17 Research Foundation Of The City University Of New York Convertible nucleoside derivatives
US20090007775A1 (en) * 2007-03-07 2009-01-08 Seymour John C Engine for Aeronautical Applications II
CN102344477B (zh) * 2010-07-27 2015-04-08 苏州瑞博生物技术有限公司 一种核苷酸和/或寡核苷酸及其制备方法
WO2017156391A1 (en) * 2016-03-11 2017-09-14 Spring Bank Pharmaceuticals, Inc. Compounds and compositions for the treatment of infections
WO2019158007A1 (zh) * 2018-02-13 2019-08-22 深圳华大生命科学研究院 合成寡核苷酸的方法及系统
EP3810317A4 (de) * 2018-05-29 2022-03-02 Centrillion Technology Holdings Corporation Verfahren und systeme zur überwachung der stufenweisen oligonukleotidsynthese in fester phase
CN117377764A (zh) * 2021-02-08 2024-01-09 中美瑞康核酸技术(南通)研究院有限公司 多价寡核苷酸试剂及其使用方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
US5214134A (en) * 1990-09-12 1993-05-25 Sterling Winthrop Inc. Process of linking nucleosides with a siloxane bridge
ATE150464T1 (de) * 1993-01-08 1997-04-15 Hybridon Inc Synthese von dimer-blöcken und ihre verwendung bei der zusammensetzung von oligonukleotiden
US5856464A (en) * 1995-06-07 1999-01-05 Lajolla Pharmaceutical Company Selective capping solution phase oligonucleotide synthesis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays

Also Published As

Publication number Publication date
DE69609184D1 (de) 2000-08-10
WO1996040708A2 (en) 1996-12-19
CA2222440A1 (en) 1996-12-19
US6166198A (en) 2000-12-26
EP0830366B1 (de) 2000-07-05
JPH11510790A (ja) 1999-09-21
AU694359B2 (en) 1998-07-16
ATE194355T1 (de) 2000-07-15
AU5752496A (en) 1996-12-30
EP0830366A2 (de) 1998-03-25
KR19990022711A (ko) 1999-03-25
US5856464A (en) 1999-01-05
WO1996040708A3 (en) 1997-02-20
CN1190973A (zh) 1998-08-19

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Legal Events

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8339 Ceased/non-payment of the annual fee