WO2001048184A3 - Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche - Google Patents

Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche Download PDF

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Publication number
WO2001048184A3
WO2001048184A3 PCT/EP2000/013157 EP0013157W WO0148184A3 WO 2001048184 A3 WO2001048184 A3 WO 2001048184A3 EP 0013157 W EP0013157 W EP 0013157W WO 0148184 A3 WO0148184 A3 WO 0148184A3
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WO
WIPO (PCT)
Prior art keywords
nucleotide
nucleic acids
carrying
nucleic acid
acid mixture
Prior art date
Application number
PCT/EP2000/013157
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English (en)
French (fr)
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WO2001048184A2 (de
Inventor
Achim Fischer
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Axaron Bioscience Ag
Achim Fischer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19962893A external-priority patent/DE19962893A1/de
Priority claimed from DE2000151564 external-priority patent/DE10051564A1/de
Application filed by Axaron Bioscience Ag, Achim Fischer filed Critical Axaron Bioscience Ag
Priority to EP00990813A priority Critical patent/EP1244782A2/de
Priority to AU30158/01A priority patent/AU3015801A/en
Publication of WO2001048184A2 publication Critical patent/WO2001048184A2/de
Publication of WO2001048184A3 publication Critical patent/WO2001048184A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur parallelen Sequenzierung von mindestens zwei verschiedenen in einem Nuckleisäuregemisch enthalten Nukleinsäuren, wobei (a) eine Oberfläche bereitgestellt wird, aufweisend Inseln von Nukleinsäuren jeweils gleicher Sorte, tertiäre Nukleinsäuren; (b) Gegenstränge der tertiären Nukleinsäuren, GTN, bereitgestellt werden; (c) die GTN um ein Nukleotid verlängert werden, wobei das Nukleotid an der 2'-OH-Position oder an der 3'-OH-Position eine Schutzgruppe trägt, die eine weitere Verlängerung verhindert, das Nukleotid eine Molekülgruppe trägt, die die Identifikation des Nukleotids ermöglicht; (d) das eingebaute Nukleotid identifiziert wird; (e) die Schuztgruppe entfernt wird und die zur Identifikation verwendete Molekülgruppe des eingebauten Nukleotids entfernt oder verändert wird, und (f) Schritt (c) und nachfolgende Schritte solange wiederholt werden, bis die gewünschte Sequenzinformation erhalten worden ist.
PCT/EP2000/013157 1999-12-23 2000-12-22 Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche WO2001048184A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00990813A EP1244782A2 (de) 1999-12-23 2000-12-22 Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
AU30158/01A AU3015801A (en) 1999-12-23 2000-12-22 Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19962893.9 1999-12-23
DE19962893A DE19962893A1 (de) 1999-12-23 1999-12-23 Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche
DE2000151564 DE10051564A1 (de) 2000-10-18 2000-10-18 Neue Verfahren zur parallelen Sequenzierung eines Nukleinsäuregemisches an einer Oberfläche
DE10051564.9 2000-10-18

Publications (2)

Publication Number Publication Date
WO2001048184A2 WO2001048184A2 (de) 2001-07-05
WO2001048184A3 true WO2001048184A3 (de) 2002-05-16

Family

ID=26007394

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/013157 WO2001048184A2 (de) 1999-12-23 2000-12-22 Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche

Country Status (4)

Country Link
US (1) US20030186256A1 (de)
EP (1) EP1244782A2 (de)
AU (1) AU3015801A (de)
WO (1) WO2001048184A2 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
WO2002029003A2 (en) 2000-10-06 2002-04-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
DE10106320A1 (de) * 2001-02-09 2002-08-22 Axaron Bioscience Ag Erzeugung und Verwendung von Zufallsanordnungen klonaler Nukleinsäureinseln auf einer Oberfläche
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
DE10224339A1 (de) * 2002-05-29 2003-12-11 Axaron Bioscience Ag Verfahren zur hochparallelen Nukleinsäuresequenzierung
SI3002289T1 (en) 2002-08-23 2018-07-31 Illumina Cambridge Limited MODIFIED NUCLEOTES FOR POLYUCULOTIDE SEQUENCING
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
WO2006138284A2 (en) 2005-06-15 2006-12-28 Callida Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0517097D0 (en) * 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8278047B2 (en) 2007-10-01 2012-10-02 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
EP3431615A3 (de) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit nichtfluoreszierenden, reversiblen nukleotidterminatoren und spaltbaren, etikettmodifizierten nukleotidterminatoren
WO2010028140A2 (en) 2008-09-03 2010-03-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8455260B2 (en) 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
EP2426214A1 (de) 2010-09-01 2012-03-07 Koninklijke Philips Electronics N.V. Verfahren zur Amplifikation von Nukleinsäuren
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
EP2640849B1 (de) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Verfahren zur sequenzierung von biomolekülen durch nachweis der relativen positionen von hybridisierten sonden
WO2012109574A2 (en) * 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
EP2956550B1 (de) 2013-01-18 2020-04-08 Nabsys 2.0 LLC Verbesserte sondenanbindung
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
JP6333297B2 (ja) 2013-03-15 2018-05-30 イルミナ ケンブリッジ リミテッド 修飾ヌクレオシドまたは修飾ヌクレオチド

Citations (7)

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Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1996023807A1 (en) * 1995-01-31 1996-08-08 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5798210A (en) * 1993-03-26 1998-08-25 Institut Pasteur Derivatives utilizable in nucleic acid sequencing

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Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
US5798210A (en) * 1993-03-26 1998-08-25 Institut Pasteur Derivatives utilizable in nucleic acid sequencing
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
WO1996023807A1 (en) * 1995-01-31 1996-08-08 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation

Also Published As

Publication number Publication date
US20030186256A1 (en) 2003-10-02
AU3015801A (en) 2001-07-09
WO2001048184A2 (de) 2001-07-05
EP1244782A2 (de) 2002-10-02

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