DE69513579T2 - Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen - Google Patents
Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von EnzymenInfo
- Publication number
- DE69513579T2 DE69513579T2 DE69513579T DE69513579T DE69513579T2 DE 69513579 T2 DE69513579 T2 DE 69513579T2 DE 69513579 T DE69513579 T DE 69513579T DE 69513579 T DE69513579 T DE 69513579T DE 69513579 T2 DE69513579 T2 DE 69513579T2
- Authority
- DE
- Germany
- Prior art keywords
- glycosaminoglycan
- derivative
- gel
- acrylamide
- degrading enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, e.g. Konjac gum, Locust bean gum, Guar gum
- G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19128594 | 1994-07-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69513579D1 DE69513579D1 (de) | 2000-01-05 |
DE69513579T2 true DE69513579T2 (de) | 2000-04-27 |
Family
ID=16272027
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69513579T Expired - Fee Related DE69513579T2 (de) | 1994-07-22 | 1995-07-21 | Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen |
Country Status (4)
Country | Link |
---|---|
US (1) | US6228998B1 (de) |
EP (1) | EP0693499B1 (de) |
AT (1) | ATE187178T1 (de) |
DE (1) | DE69513579T2 (de) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19818077A1 (de) * | 1998-04-22 | 1999-10-28 | Biotechnolog Forschung Gmbh | Immobilisierte Substrate, damit gefertigte Trenngele und Verfahren zum Nachweis von Enzymaktivitäten nach elektrophoretischer Protein-Trennung |
JP3493608B2 (ja) * | 2001-11-28 | 2004-02-03 | 学校法人慶應義塾 | 医用高分子及びその用途 |
EP2402753A1 (de) | 2002-03-11 | 2012-01-04 | Momenta Pharmaceuticals, Inc. | Analyse von sulfatierten Polysacchariden |
US8673333B2 (en) | 2002-09-25 | 2014-03-18 | The Johns Hopkins University | Cross-linked polymer matrices, and methods of making and using same |
US9139876B1 (en) | 2007-05-03 | 2015-09-22 | Momenta Pharmacueticals, Inc. | Method of analyzing a preparation of a low molecular weight heparin |
GB0720030D0 (en) * | 2007-10-12 | 2007-11-21 | Cambridge Entpr Ltd | Depolymerase assay |
ES2817779T3 (es) * | 2010-01-19 | 2021-04-08 | Momenta Pharmaceuticals Inc | Evaluación de preparaciones de heparina |
WO2012115952A1 (en) | 2011-02-21 | 2012-08-30 | Momenta Pharmaceuticals, Inc. | Evaluating heparin preparations |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1206666A (en) | 1982-02-19 | 1986-06-24 | Samuel Nochumson | Copolymer of acrylamide and polysaccharide resin as electrophoretic gel medium |
US4859581A (en) * | 1986-03-10 | 1989-08-22 | Board Of Regents, The University Of Texas System | Endoglycosidase assay |
JP2726275B2 (ja) * | 1988-08-24 | 1998-03-11 | 生化学工業株式会社 | グリコサミノグリカン分解酵素の精製法 |
JP2975632B2 (ja) | 1990-03-30 | 1999-11-10 | 生化学工業株式会社 | グリコサミノグリカン修飾プロテイン |
AU647814B2 (en) | 1990-07-24 | 1994-03-31 | Seikagaku Kogyo Kabushiki Kaisha | Phospholipid- or lipid-combining glycosaminoglycan, production thereof, and cancer metastasis inhibitor |
-
1995
- 1995-07-20 US US08/504,675 patent/US6228998B1/en not_active Expired - Lifetime
- 1995-07-21 DE DE69513579T patent/DE69513579T2/de not_active Expired - Fee Related
- 1995-07-21 EP EP95111522A patent/EP0693499B1/de not_active Expired - Lifetime
- 1995-07-21 AT AT95111522T patent/ATE187178T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0693499A1 (de) | 1996-01-24 |
US6228998B1 (en) | 2001-05-08 |
DE69513579D1 (de) | 2000-01-05 |
ATE187178T1 (de) | 1999-12-15 |
EP0693499B1 (de) | 1999-12-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69926677D1 (de) | Verfahren zum Vernetzen von Proteinen mittels Enzyme | |
DE3481775D1 (de) | Verfahren zum raschen nachweis von mikroorganismen in klinischen proben. | |
NZ335107A (en) | Trichoderma cellulase isolated from a culture of Trichoderma longibrachiatum with an estimated molecular weight of 95-105 kD and an estimated pH optimum of 5.0 at 338 Kelvin and ph 4.0 at 318 and 328 Kelvin | |
EA199900140A1 (ru) | Гетероциклические эфиры и амиды | |
DE69736981D1 (de) | In vitro verfahren zum einbringen von nukleinsäuren in eine zelle | |
EA199900182A1 (ru) | Гетероциклические тиоэфиры и кетоны | |
DE68901285D1 (de) | Verfahren zum nachweis von nukleotid-sequenzen. | |
ATE109513T1 (de) | Verfahren zur reinigung von rekombinanten proteinen und die verwendung von dessen produkten. | |
ATE439590T1 (de) | Verfahren zum selektiven testen von adiponectinmultimeren | |
DE69523179D1 (de) | Verfahren zum nachweis eines genetischen defektes, assoziert mit thrombose und/oder mangelhafter anticoagulanz-reaktion auf aktiviertes protein c | |
DE50015331D1 (de) | Verfahren zum anbinden von nukleinsäuren an eine festphase | |
DE69900462D1 (de) | Konjugate von dns und pns und verfahren zu deren herstellung | |
ATE43640T1 (de) | Zusammensetzung und testvorrichtung zum nachweis von leukozyten, esterase und protease in einer testprobe. | |
DE69513579D1 (de) | Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen | |
DE69834506D1 (de) | Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung | |
ATE220112T1 (de) | Verfahren zum nachweis von störungen des protein c/protein s-systems | |
EP1177420A4 (de) | MIT FTA BESCHICHTETER TRäGER ZUR VERWENDUNG ALS MOLEKULARES DIAGNOSEMITTEL | |
ATE184879T1 (de) | Verfahren und material für reinigung von nukleinsäuren | |
ATE150664T1 (de) | Verbessertes zweistoffgel aus galactomannan- agarose für die elektrophorese von nukleinsäure | |
ATE450796T1 (de) | Teststäbchen zur verwendung beim nachweis von mikroorganismen | |
DE68925457D1 (de) | Diagnoseverfahren für infektionskrankheiten sowie verfahren zum nachweis und identifizierung von mikroorganismen | |
DE68904578T2 (de) | Enzym zur alpha-amidierung von c-termini und verfahren zu dessen herstellung. | |
ES8900085A1 (es) | Procedimiento para la obtencion de composiciones o preparaciones para controlar el desprendimiento de microorganismos adheridos a superficies | |
DE69514549D1 (de) | Verfahren zum erhalt von biomasse aus mikroorganismen mit geringem nukleinsäuregehalt | |
JPS6486877A (en) | Decision gene dna of vibrio anguillarum and pasteurella piscida species and uses thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |