DE69513579D1 - Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen - Google Patents

Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen

Info

Publication number
DE69513579D1
DE69513579D1 DE69513579T DE69513579T DE69513579D1 DE 69513579 D1 DE69513579 D1 DE 69513579D1 DE 69513579 T DE69513579 T DE 69513579T DE 69513579 T DE69513579 T DE 69513579T DE 69513579 D1 DE69513579 D1 DE 69513579D1
Authority
DE
Germany
Prior art keywords
glycosaminoglycan
derivative
gel
acrylamide
degrading enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69513579T
Other languages
English (en)
Other versions
DE69513579T2 (de
Inventor
Ryu Miura
Sadako Yamagata
Tatsuya Yamagata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Publication of DE69513579D1 publication Critical patent/DE69513579D1/de
Application granted granted Critical
Publication of DE69513579T2 publication Critical patent/DE69513579T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, e.g. Konjac gum, Locust bean gum, Guar gum
    • G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Materials Engineering (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Dermatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
DE69513579T 1994-07-22 1995-07-21 Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen Expired - Fee Related DE69513579T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP19128594 1994-07-22

Publications (2)

Publication Number Publication Date
DE69513579D1 true DE69513579D1 (de) 2000-01-05
DE69513579T2 DE69513579T2 (de) 2000-04-27

Family

ID=16272027

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69513579T Expired - Fee Related DE69513579T2 (de) 1994-07-22 1995-07-21 Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen

Country Status (4)

Country Link
US (1) US6228998B1 (de)
EP (1) EP0693499B1 (de)
AT (1) ATE187178T1 (de)
DE (1) DE69513579T2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19818077A1 (de) * 1998-04-22 1999-10-28 Biotechnolog Forschung Gmbh Immobilisierte Substrate, damit gefertigte Trenngele und Verfahren zum Nachweis von Enzymaktivitäten nach elektrophoretischer Protein-Trennung
JP3493608B2 (ja) * 2001-11-28 2004-02-03 学校法人慶應義塾 医用高分子及びその用途
EP2402753A1 (de) 2002-03-11 2012-01-04 Momenta Pharmaceuticals, Inc. Analyse von sulfatierten Polysacchariden
US8673333B2 (en) 2002-09-25 2014-03-18 The Johns Hopkins University Cross-linked polymer matrices, and methods of making and using same
US9139876B1 (en) 2007-05-03 2015-09-22 Momenta Pharmacueticals, Inc. Method of analyzing a preparation of a low molecular weight heparin
GB0720030D0 (en) * 2007-10-12 2007-11-21 Cambridge Entpr Ltd Depolymerase assay
US8435795B2 (en) * 2010-01-19 2013-05-07 Momenta Pharmaceuticals, Inc. Evaluating heparin preparations
US9068957B2 (en) 2011-02-21 2015-06-30 Momenta Pharmaceuticals, Inc. Evaluating heparin preparations

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1206666A (en) 1982-02-19 1986-06-24 Samuel Nochumson Copolymer of acrylamide and polysaccharide resin as electrophoretic gel medium
US4859581A (en) 1986-03-10 1989-08-22 Board Of Regents, The University Of Texas System Endoglycosidase assay
JP2726275B2 (ja) * 1988-08-24 1998-03-11 生化学工業株式会社 グリコサミノグリカン分解酵素の精製法
JP2975632B2 (ja) * 1990-03-30 1999-11-10 生化学工業株式会社 グリコサミノグリカン修飾プロテイン
WO1992001720A1 (en) 1990-07-24 1992-02-06 Seikagaku Kogyo Kabushiki Kaisha Phospholipid- or lipid-combining glycosaminoglycan, production thereof, and cancer metastasis inhibitor

Also Published As

Publication number Publication date
ATE187178T1 (de) 1999-12-15
EP0693499A1 (de) 1996-01-24
EP0693499B1 (de) 1999-12-01
US6228998B1 (en) 2001-05-08
DE69513579T2 (de) 2000-04-27

Similar Documents

Publication Publication Date Title
DE69926677D1 (de) Verfahren zum Vernetzen von Proteinen mittels Enzyme
DE3481775D1 (de) Verfahren zum raschen nachweis von mikroorganismen in klinischen proben.
NZ335107A (en) Trichoderma cellulase isolated from a culture of Trichoderma longibrachiatum with an estimated molecular weight of 95-105 kD and an estimated pH optimum of 5.0 at 338 Kelvin and ph 4.0 at 318 and 328 Kelvin
EA199900140A1 (ru) Гетероциклические эфиры и амиды
DE69736981D1 (de) In vitro verfahren zum einbringen von nukleinsäuren in eine zelle
EA199900182A1 (ru) Гетероциклические тиоэфиры и кетоны
DE68901285D1 (de) Verfahren zum nachweis von nukleotid-sequenzen.
DE69523179T2 (de) Verfahren zum nachweis eines genetischen defektes, assoziert mit thrombose und/oder mangelhafter anticoagulanz-reaktion auf aktiviertes protein c
DE50015331D1 (de) Verfahren zum anbinden von nukleinsäuren an eine festphase
DE69900462D1 (de) Konjugate von dns und pns und verfahren zu deren herstellung
DE602004022575D1 (de) Verfahren zum selektiven testen von adiponectinmultimeren
ATE43640T1 (de) Zusammensetzung und testvorrichtung zum nachweis von leukozyten, esterase und protease in einer testprobe.
DE69513579D1 (de) Gel auf Basis eines Derivat von Glykosaminoglykan; Copolymers aus Acrylamid und diesem Derivat; Verfahren zum Nachweis von Enzymen
ATE325893T1 (de) Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung
HUP0100668A2 (hu) Virulenciával kapcsolatos nukleinsavszekvenciák és alkalmazásuk
ATE220112T1 (de) Verfahren zum nachweis von störungen des protein c/protein s-systems
EP1177420A4 (de) MIT FTA BESCHICHTETER TRäGER ZUR VERWENDUNG ALS MOLEKULARES DIAGNOSEMITTEL
ATE184879T1 (de) Verfahren und material für reinigung von nukleinsäuren
EA199900511A1 (ru) N-связанные мочевины и карбаматы сложных гетероциклических тиоэфиров
DE69218586D1 (de) Verbessertes zweistoffgel aus galactomannan-agarose für die elektrophorese von nukleinsäure
ATE450796T1 (de) Teststäbchen zur verwendung beim nachweis von mikroorganismen
DE68925457D1 (de) Diagnoseverfahren für infektionskrankheiten sowie verfahren zum nachweis und identifizierung von mikroorganismen
DE68904578T2 (de) Enzym zur alpha-amidierung von c-termini und verfahren zu dessen herstellung.
ES8802556A1 (es) Procedimiento para controlar el desprendimiento de microorganismos adheridos a superficies
DE69514549D1 (de) Verfahren zum erhalt von biomasse aus mikroorganismen mit geringem nukleinsäuregehalt

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee