DE69435234D1 - Verfahren zur in vitro Aktivierung der Zellkerne - Google Patents

Verfahren zur in vitro Aktivierung der Zellkerne

Info

Publication number
DE69435234D1
DE69435234D1 DE69435234T DE69435234T DE69435234D1 DE 69435234 D1 DE69435234 D1 DE 69435234D1 DE 69435234 T DE69435234 T DE 69435234T DE 69435234 T DE69435234 T DE 69435234T DE 69435234 D1 DE69435234 D1 DE 69435234D1
Authority
DE
Germany
Prior art keywords
cell nuclei
extracts
vitro activation
analyzing
activating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69435234T
Other languages
English (en)
Inventor
Lawrence J Wangh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Application granted granted Critical
Publication of DE69435234D1 publication Critical patent/DE69435234D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69435234T 1993-02-03 1994-02-01 Verfahren zur in vitro Aktivierung der Zellkerne Expired - Fee Related DE69435234D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/013,039 US5480772A (en) 1993-02-03 1993-02-03 In vitro activation of a nucleus

Publications (1)

Publication Number Publication Date
DE69435234D1 true DE69435234D1 (de) 2009-10-08

Family

ID=21757999

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69435234T Expired - Fee Related DE69435234D1 (de) 1993-02-03 1994-02-01 Verfahren zur in vitro Aktivierung der Zellkerne
DE69434722T Expired - Fee Related DE69434722T2 (de) 1993-02-03 1994-02-01 In vitro Verfahren zur Induzierung der nukleären Schwellung

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69434722T Expired - Fee Related DE69434722T2 (de) 1993-02-03 1994-02-01 In vitro Verfahren zur Induzierung der nukleären Schwellung

Country Status (9)

Country Link
US (8) US5480772A (de)
EP (2) EP1554931B1 (de)
AT (2) ATE440494T1 (de)
CA (1) CA2155309A1 (de)
DE (2) DE69435234D1 (de)
DK (1) DK0686202T3 (de)
ES (1) ES2267095T3 (de)
PT (1) PT686202E (de)
WO (1) WO1994018344A1 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480772A (en) * 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
JP3834080B2 (ja) * 1994-08-12 2006-10-18 英介 後藤 蛋白質脱リン酸化酵素阻害剤を主成分とする未成熟凝縮染色体生成剤と生成方法
US6033849A (en) * 1995-05-26 2000-03-07 The Salk Institute For Biological Studies Method for isolation of extrachromosomal amplified genes
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US7332648B2 (en) 1995-08-31 2008-02-19 Roslin Institute Unactivated oocytes in nuclear transfer to produce cultured inner cell mass cells and ungulates
US7361804B1 (en) 1997-02-19 2008-04-22 Roslin Institute (Edinburgh) Unactivated oocytes in nuclear transfer to produce ungulates
JP4371383B2 (ja) * 1997-11-17 2009-11-25 カーネギー インスチチューション オブ ワシントン ランプブラシ染色体の生産
US6331659B1 (en) 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
US6281005B1 (en) 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
US7064245B1 (en) * 1999-06-04 2006-06-20 Istituto Zootecnico E Caseario Per La Sardegna Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat treated donor nucleus and preparaing a non-human mammal
US20040199935A1 (en) * 1999-06-30 2004-10-07 Chapman Karen B. Cytoplasmic transfer to de-differentiate recipient cells
JP2003503071A (ja) 1999-06-30 2003-01-28 アドバンスド セル テクノロジー、インコーポレイテッド 脱分化レシピエント細胞への細胞質移入
US20110171185A1 (en) * 1999-06-30 2011-07-14 Klimanskaya Irina V Genetically intact induced pluripotent cells or transdifferentiated cells and methods for the production thereof
FR2799099B1 (fr) * 1999-10-01 2001-12-21 Agronomique Inst Nat Rech Methode de reconstitution d'un embryon de mammifere non humain avec des cellules differenciees foetales et adultes
AU1581001A (en) * 1999-11-02 2001-05-14 University Of Massachusetts Use of haploid genomes for genetic diagnosis, modification and multiplication
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
WO2001035735A1 (en) * 1999-11-19 2001-05-25 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
ATE517990T1 (de) * 2000-03-15 2011-08-15 Univ Georgia Effektive kernumprogrammierung in säugetieren
AU2001284703B2 (en) 2000-08-03 2007-03-22 Therapeutic Human Polyclonals Inc. Production of humanized antibodies in transgenic animals
GB0022981D0 (en) * 2000-09-19 2000-11-01 St Georges Entpr Ltd Manipulation of spermatozoa
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
EP1356035B1 (de) 2000-12-22 2011-03-16 Kyowa Hakko Kirin Co., Ltd. Verfahren zur klonierung von nichtmenschliche säugern unter verwendung von reprogrammiertem donorchromatin oder donorzellen
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
CA2450652A1 (en) * 2001-06-14 2002-12-27 Infigen, Inc. Methods for cloning mammals using remodeling factors
US20030211603A1 (en) * 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
AU2002313817A1 (en) * 2001-08-27 2003-03-10 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
US20030044976A1 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
US7138227B2 (en) * 2001-09-27 2006-11-21 Hirokazu Kusakabe Frozen spermatozoa compositions and uses thereof
US20040210955A1 (en) * 2001-10-26 2004-10-21 Hidenori Akutsu Preparation of spermatozoa for ICSI-mediated transgenesis and methods of using the same
EP1391503A1 (de) * 2002-08-12 2004-02-25 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verfahren zur Umprogrammierung einer Zelle durch zytoplasmatichen Transfer
AU2003290689A1 (en) * 2002-11-08 2004-06-03 Kyowa Hakko Kirin Co., Ltd. Transgenic ungulates having reduced prion protein activity and uses thereof
WO2005049788A2 (en) * 2003-01-17 2005-06-02 University Of Massachusetts Reprogramming of somatic cell nuclei
US7904365B2 (en) 2003-03-03 2011-03-08 Itg Software Solutions, Inc. Minimizing security holdings risk during portfolio trading
WO2005024385A2 (en) * 2003-09-09 2005-03-17 Biogenex Laboratories Sample processing system
GB0329608D0 (en) * 2003-12-22 2004-01-28 Univ Nottingham A novel method for embryo and animal production
CA2852122A1 (en) 2004-01-02 2005-07-28 Advanced Cell Technology, Inc. Novel culture systems for ex vivo development
CN100519741C (zh) * 2004-04-03 2009-07-29 成都军区昆明总医院 动物卵细胞提取物
US7420099B2 (en) * 2004-04-22 2008-09-02 Kirin Holdings Kabushiki Kaisha Transgenic animals and uses thereof
US20060252087A1 (en) * 2005-01-18 2006-11-09 Biocept, Inc. Recovery of rare cells using a microchannel apparatus with patterned posts
US20090136982A1 (en) 2005-01-18 2009-05-28 Biocept, Inc. Cell separation using microchannel having patterned posts
CN103382434B (zh) 2005-01-18 2016-05-25 生物概念股份有限公司 利用含排列成图案的立柱的微通道分离细胞
FR2885368A1 (fr) * 2005-05-04 2006-11-10 Fred Zacouto Systemes et procedes de regeneration cellulaire et utilisations de telles cellules regenerees
US20100167404A1 (en) * 2005-08-03 2010-07-01 Advanced Cell Technology, Inc. Methods of Reprogramming Animal Somatic Cells
CA2624144C (en) * 2005-09-30 2016-07-19 Centre National De La Recherche Scientifique Use of a cellular extract for a mitotic remodeling of chromosomes
US20070102389A1 (en) * 2005-11-08 2007-05-10 Seaquist Closures Foreign, Inc. Tamper-evident closure
US7695956B2 (en) * 2006-01-12 2010-04-13 Biocept, Inc. Device for cell separation and analysis and method of using
US9551635B2 (en) 2006-03-09 2017-01-24 Biogenex Laboratories Inc. Sample processing system
EP2101724B1 (de) 2006-05-11 2020-12-02 Regenics AS Verabreichung von zellextrakten zur verjüngung
JP5087625B2 (ja) 2006-09-01 2012-12-05 セラピューティック・ヒューマン・ポリクローナルズ・インコーポレーテッド 非ヒトトランスジェニック動物におけるヒトまたはヒト化免疫グロブリンの発現強化
WO2008030607A2 (en) * 2006-09-08 2008-03-13 Statspin, Inc. Centrifugal device and method for ova detection
US20090053722A1 (en) * 2007-08-21 2009-02-26 David Brown Sdad assay and uses thereof
US20100323441A1 (en) * 2007-12-13 2010-12-23 Biocell Center S.P.A. Method of collection and preservation of fluids and/or materials, in particular of organic fluids and/or materials containing stem cells, and device employable in such method
EP2296676B1 (de) 2008-05-09 2016-11-02 Regenics AS Zellextrakte
US7943187B2 (en) * 2008-05-23 2011-05-17 Bakr Rabie Paeoniflorin preparations and uses thereof for fat reduction
US8819686B2 (en) * 2009-07-23 2014-08-26 Empire Technology Development Llc Scheduling threads on different processor cores based on memory temperature
AU2011249478B2 (en) 2010-05-06 2014-12-04 Regenics As Use of cellular extracts for skin rejuvenation
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
CA2894448C (en) 2012-12-10 2019-09-17 Regenics As Use of cellular extracts for skin rejuvenation
US20170045427A1 (en) * 2014-02-20 2017-02-16 The Arizona Board Of Regents On Behalf Of The University Of Arizona Method for guiding cell spreading in automated cytogenetic assays
CA2994304A1 (en) * 2015-08-20 2017-02-23 Genomatica, Inc. Compositions and multiplexed systems for coupled cell-free transcription-translation and protein synthesis and methods for using them
US20200347449A1 (en) * 2019-05-01 2020-11-05 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) * 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4722598A (en) * 1986-12-04 1988-02-02 Max M. Ford Diagnostic microscope slide having multiple sample wells and cover
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US4865812A (en) * 1987-02-03 1989-09-12 Davstar Industries, Inc. Integral specimen collection tube and microscope slide device
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5213979A (en) 1987-12-30 1993-05-25 W. R. Grace & Co.-Conn. In vitro culture of bovine embryos
US5258847A (en) * 1989-03-22 1993-11-02 Canon Kabushiki Kaisha Object tracing device
US5358847A (en) * 1991-10-24 1994-10-25 Brown David B Method of screening for human sperm abnormalities as part of a regimen for assessing fertilizing capacity based upon reduced rates of chromatin decondensation and DNA synthesis
JPH06125772A (ja) 1992-03-04 1994-05-10 Wr Grace & Co Connecticut 牛の核移入の改良工程
CA2092258A1 (en) 1992-12-30 1994-07-01 Steven L. Stice Synchronized donor cells for bovine nuclear transfer
US5480772A (en) * 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
AU1439295A (en) 1993-12-17 1995-07-03 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
EP1149898A2 (de) 1993-12-23 2001-10-31 Infigen, Inc. Embryonale Stammzellen von Huftieren als Kerndonatoren und Kerntransfertechniken zur Herstellung chimärer und transgener Tiere
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5905042A (en) 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
NZ334016A (en) 1996-08-19 2000-08-25 Univ Massachusetts Embryonic stem cell lines produced by cross species nuclear transplantation

Also Published As

Publication number Publication date
US20060021076A9 (en) 2006-01-26
DK0686202T3 (da) 2006-09-04
US20040177397A1 (en) 2004-09-09
EP1554931A1 (de) 2005-07-20
US5773217A (en) 1998-06-30
US20020178462A1 (en) 2002-11-28
ATE325204T1 (de) 2006-06-15
WO1994018344A1 (en) 1994-08-18
US20050055734A1 (en) 2005-03-10
ATE440494T1 (de) 2009-09-15
EP0686202B1 (de) 2006-05-03
ES2267095T3 (es) 2007-03-01
US6753457B2 (en) 2004-06-22
EP0686202A1 (de) 1995-12-13
US6245567B1 (en) 2001-06-12
DE69434722T2 (de) 2007-03-08
US20020132344A1 (en) 2002-09-19
CA2155309A1 (en) 1994-08-18
DE69434722D1 (de) 2006-06-08
US6878546B2 (en) 2005-04-12
US5651992A (en) 1997-07-29
PT686202E (pt) 2006-10-31
EP0686202A4 (de) 1997-05-07
US5480772A (en) 1996-01-02
EP1554931B1 (de) 2009-08-26

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