DE69434953D1 - Verfahren zur Herstellung einer cDNA Bank - Google Patents

Verfahren zur Herstellung einer cDNA Bank

Info

Publication number
DE69434953D1
DE69434953D1 DE69434953T DE69434953T DE69434953D1 DE 69434953 D1 DE69434953 D1 DE 69434953D1 DE 69434953 T DE69434953 T DE 69434953T DE 69434953 T DE69434953 T DE 69434953T DE 69434953 D1 DE69434953 D1 DE 69434953D1
Authority
DE
Germany
Prior art keywords
cdna library
preparation
polypeptide
useful
signal peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434953T
Other languages
English (en)
Inventor
Tasuku Honjo
Kei Tashiro
Hideaki Tada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ono Pharmaceutical Co Ltd
Original Assignee
Ono Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=12073412&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69434953(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ono Pharmaceutical Co Ltd filed Critical Ono Pharmaceutical Co Ltd
Application granted granted Critical
Publication of DE69434953D1 publication Critical patent/DE69434953D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
DE69434953T 1993-01-14 1994-01-14 Verfahren zur Herstellung einer cDNA Bank Expired - Lifetime DE69434953D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2209893 1993-01-14

Publications (1)

Publication Number Publication Date
DE69434953D1 true DE69434953D1 (de) 2007-05-24

Family

ID=12073412

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69431736T Revoked DE69431736T2 (de) 1993-01-14 1994-01-14 Neues Chemokine von hematopoietischen Zellen produziert und die dafür kodierende DNA
DE69434953T Expired - Lifetime DE69434953D1 (de) 1993-01-14 1994-01-14 Verfahren zur Herstellung einer cDNA Bank

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69431736T Revoked DE69431736T2 (de) 1993-01-14 1994-01-14 Neues Chemokine von hematopoietischen Zellen produziert und die dafür kodierende DNA

Country Status (10)

Country Link
US (1) US5525486A (de)
EP (2) EP1243650B1 (de)
JP (2) JP2879303B2 (de)
KR (1) KR100271725B1 (de)
AT (2) ATE359366T1 (de)
CA (2) CA2381458A1 (de)
DE (2) DE69431736T2 (de)
DK (1) DK0607054T3 (de)
ES (1) ES2187516T3 (de)
PT (1) PT607054E (de)

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US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
CA2117953C (en) * 1993-10-14 2001-12-11 Tasuku Honjo Human stromal derived factor 1 alpha and 1 beta and dnas encoding the same
WO1997023640A1 (en) * 1995-12-26 1997-07-03 Human Genome Sciences, Inc. Human chemotactic cytokine i
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO1996001843A1 (fr) * 1994-07-08 1996-01-25 Ono Pharmaceutical Co., Ltd. Polypeptide produit par des cellules du stroma
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
US5665590A (en) * 1994-07-29 1997-09-09 Systemix, Inc. Method for isolating and directly cloning genes which encode cell-surface and secreted proteins
EP0832209A1 (de) * 1995-06-07 1998-04-01 ZymoGenetics, Inc. Methode zum einfangen und klonieren der zur förderung der sekretion notwendigen leitsequenzen
US5753462A (en) * 1995-06-07 1998-05-19 Zymogenetics, Inc. Secretion leader trap cloning method
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US7393943B1 (en) 1995-12-08 2008-07-01 Human Genome Sciences, Inc. Polynucleotides encoding a human chemotactic cytokine I
WO1997025443A1 (en) * 1996-01-10 1997-07-17 The University Of Liverpool Metastasis inducing dna's
WO1997029211A1 (en) * 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
US5952197A (en) * 1996-03-05 1999-09-14 Human Genome Sciences, Inc. DNA encoding the chemotactic cytokine III
AU5523496A (en) * 1996-03-05 1997-09-22 Human Genome Sciences, Inc. Chemotactic cytokine iii
AU5420796A (en) * 1996-03-11 1997-10-01 Human Genome Sciences, Inc. Chemotactic cytokine ii
AU5425496A (en) * 1996-03-18 1997-10-10 Human Genome Sciences, Inc. Chemokine alpha 3
US6410268B1 (en) * 1996-03-18 2002-06-25 Human Genome Sciences, Inc. Polynucleotides encoding chemokine alpha-3
NZ332318A (en) * 1996-03-19 2000-10-27 Human Genome Sciences Inc Chemokine alpha 2 and it's therapeutic and diagnostic use in treating illnesses
WO1997040146A1 (en) * 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
EP1170366A1 (de) * 1996-04-24 2002-01-09 Genetics Institute, Inc. Secreted proteins obtained by using Yeast invertase gene as reporter system
JP2002238557A (ja) 1996-07-23 2002-08-27 Fujisawa Pharmaceut Co Ltd トランスメンブレントラップ法
US5891637A (en) * 1996-10-15 1999-04-06 Genentech, Inc. Construction of full length cDNA libraries
US5942422A (en) * 1996-11-14 1999-08-24 The Trustees Of Columbia University In The City Of New York Method for generating a directed, recombinant fusion nucleic acid
US5952171A (en) 1996-11-19 1999-09-14 Millennium Biotherapeutics, Inc. Method for identifying genes encoding secreted or membrane-associated proteins
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
DE69842225D1 (de) * 1997-04-16 2011-05-26 Millennium Pharm Inc Zusammensetzung, umfassend einen Antikörper, der selektiv an ein cysteinreiches sekretiertes Protein (CRSP) bindet
US7057017B2 (en) * 1997-04-16 2006-06-06 Millennium Pharmaceuticals, Inc. Human dickkopf-related protein and nucleic acid molecules and uses therefor
US20030083461A1 (en) 1997-06-16 2003-05-01 Genentech, Inc. Secreted and transmembrane polypeptides and nucleic acids encoding the same
DE19734161A1 (de) * 1997-08-07 1999-04-01 Jerini Biotools Gmbh SDF-1 - Antagonisten
WO1999018205A1 (fr) * 1997-10-07 1999-04-15 Ono Pharmaceutical Co., Ltd. POLYPEPTIDE, ADNc LE CODANT ET LEUR UTILISATION
US20020086364A1 (en) * 1997-10-07 2002-07-04 Ono Pharmaceutical Co., Ltd. Polypeptide, cDNA encoding the same, and use of them
US6046000A (en) * 1997-11-07 2000-04-04 Millennium Biotherapeutics, Inc. Method for identifying genes encoding signal sequences
US6866998B1 (en) 1997-11-26 2005-03-15 Chugai Seiyaku Kabushiki Kaisha Cellular assays for identifying signaling peptides
JPH11187882A (ja) 1997-12-26 1999-07-13 Ono Pharmaceut Co Ltd 新規なポリペプチド、その製造方法、そのポリペプチドをコードするｃＤＮＡ、そのｃＤＮＡからなるベクター、そのベクターで形質転換された宿主細胞、そのポリペプチドの抗体、およびそのペプチドまたは抗体を含有する薬学的組成物
US7446181B2 (en) * 1998-01-15 2008-11-04 Millennium Pharmaceuticals, Inc. Antibodies that bind human Dickkopf-1 proteins
CA2320625A1 (en) 1998-02-09 1999-08-12 Human Genome Sciences, Inc. 45 human secreted proteins
US6103472A (en) * 1998-02-20 2000-08-15 Amgen Inc. Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
US6228590B1 (en) 1998-03-23 2001-05-08 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
AU753310B2 (en) * 1998-05-20 2002-10-17 Chugai Seiyaku Kabushiki Kaisha Novel method for gene cloning
CA2328895A1 (en) * 1998-06-02 1999-12-09 Genentech, Inc. Secreted and transmembrane polypeptides and nucleic acids encoding the same
EP1090118A2 (de) * 1998-06-26 2001-04-11 Incyte Pharmaceuticals, Inc. Menschliche proteine mit signalpeptid
US7264801B2 (en) 1998-08-11 2007-09-04 Genentech, Inc. EG-VEGF nucleic acids and polypeptides and method of use
US20020172678A1 (en) 2000-06-23 2002-11-21 Napoleone Ferrara EG-VEGF nucleic acids and polypeptides and methods of use
US20030003507A1 (en) 1999-06-02 2003-01-02 Genentech, Inc. Compositions and methods for the diagnosis and treatment of tumor
US6344541B1 (en) * 1998-09-25 2002-02-05 Amgen Inc. DKR polypeptides
US6911307B1 (en) * 1998-12-08 2005-06-28 Proteus S.A. Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro
US8410248B2 (en) 1999-03-12 2013-04-02 Human Genome Sciences Inc. HWBAO62 polypeptides
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
WO2001009310A1 (en) * 1999-07-29 2001-02-08 Berlex Laboratories, Inc. 5'ENRICHED cDNA LIBRARIES AND A YEAST SIGNAL TRAP
AT410217B (de) * 2000-06-15 2003-03-25 Cistem Biotechnologies Gmbh Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen
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US20040115726A1 (en) * 2001-09-14 2004-06-17 Renpei Nagashima Method, system, apparatus and device for discovering and preparing chemical compounds for medical and other uses.
EP1402449A2 (de) * 2000-09-14 2004-03-31 Reverse Proteomics Research Institute Co., Ltd Verfahren, system, vorrichtung und gerät zur entdeckung und herstellung chemischer verbindungen
WO2002088325A2 (en) * 2001-05-02 2002-11-07 University Of South Florida Vector system for selection of genes encoding secreted proteins and membrane-bound proteins
MX2007001221A (es) * 2004-08-04 2007-03-23 Amgen Inc Anticuerpos para proteina dickkopf-1 (dkk-1).
US20130198876A1 (en) 2010-05-25 2013-08-01 National Cancer Center Induced malignant stem cells or pre-induction cancer stem cells capable of selfreplication outside of an organism, production method for same, and practical application for same
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Also Published As

Publication number Publication date
EP0607054A2 (de) 1994-07-20
PT607054E (pt) 2003-04-30
EP1243650A2 (de) 2002-09-25
JP2879303B2 (ja) 1999-04-05
ATE359366T1 (de) 2007-05-15
DE69431736D1 (de) 2003-01-02
EP0607054A3 (de) 1995-02-08
CA2113363A1 (en) 1994-07-15
EP1243650A3 (de) 2002-10-02
JPH06315380A (ja) 1994-11-15
ES2187516T3 (es) 2003-06-16
EP1243650B1 (de) 2007-04-11
US5525486A (en) 1996-06-11
ATE228163T1 (de) 2002-12-15
KR100271725B1 (ko) 2000-12-01
JPH11308993A (ja) 1999-11-09
EP0607054B1 (de) 2002-11-20
JP3229590B2 (ja) 2001-11-19
CA2381458A1 (en) 1994-07-15
DE69431736T2 (de) 2003-07-03
KR940018464A (ko) 1994-08-18
DK0607054T3 (da) 2003-03-17
CA2113363C (en) 2003-01-07

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8332 No legal effect for de