DE69434873D1 - Subtilisin bpn'-varianten mit verminderter adsorption und erhöhter hydrolyse - Google Patents

Subtilisin bpn'-varianten mit verminderter adsorption und erhöhter hydrolyse

Info

Publication number
DE69434873D1
DE69434873D1 DE69434873T DE69434873T DE69434873D1 DE 69434873 D1 DE69434873 D1 DE 69434873D1 DE 69434873 T DE69434873 T DE 69434873T DE 69434873 T DE69434873 T DE 69434873T DE 69434873 D1 DE69434873 D1 DE 69434873D1
Authority
DE
Germany
Prior art keywords
subtilisin bpn
variants
increased hydrolysis
reduced adsorption
bpn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434873T
Other languages
English (en)
Other versions
DE69434873T2 (de
Inventor
Frederick Brode
Lee Barnett
Nelton Rubingh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Procter and Gamble Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter and Gamble Co filed Critical Procter and Gamble Co
Application granted granted Critical
Publication of DE69434873D1 publication Critical patent/DE69434873D1/de
Publication of DE69434873T2 publication Critical patent/DE69434873T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
DE69434873T 1993-09-15 1994-08-31 Subtilisin bpn'-varianten mit verminderter adsorption und erhöhter hydrolyse Expired - Lifetime DE69434873T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US121437 1980-02-14
US12143793A 1993-09-15 1993-09-15
US28746194A 1994-08-11 1994-08-11
US287461 1994-08-11
PCT/US1994/010020 WO1995007991A2 (en) 1993-09-15 1994-08-31 Subtilisin bpn' variants with decreased adsorption and increased hydrolysis

Publications (2)

Publication Number Publication Date
DE69434873D1 true DE69434873D1 (de) 2006-12-07
DE69434873T2 DE69434873T2 (de) 2007-09-06

Family

ID=26819467

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434873T Expired - Lifetime DE69434873T2 (de) 1993-09-15 1994-08-31 Subtilisin bpn'-varianten mit verminderter adsorption und erhöhter hydrolyse

Country Status (14)

Country Link
EP (1) EP0719339B1 (de)
JP (1) JPH09502610A (de)
CN (1) CN1057123C (de)
AT (1) ATE343636T1 (de)
AU (1) AU7870394A (de)
BR (1) BR9407502A (de)
CA (1) CA2170491C (de)
CZ (1) CZ79196A3 (de)
DE (1) DE69434873T2 (de)
ES (1) ES2275265T3 (de)
HU (1) HUT74222A (de)
MA (1) MA23330A1 (de)
NO (1) NO961067L (de)
WO (1) WO1995007991A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
ZA952220B (en) * 1994-05-02 1995-12-14 Procter & Gamble Bpn' variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117352A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Thermitase variants having decreased adsorption and increased hydrolysis
US5718895A (en) * 1995-11-16 1998-02-17 Alcon Laboratories, Inc. Enzymes with low isoelectric points for use in contact lens cleaning
DE19617229A1 (de) * 1996-04-30 1997-11-06 Basf Ag Verfahren zur Herstellung von niedermolekularen Ethylenpolymerisaten im Hochdruck
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
EP1082442A1 (de) * 1998-03-26 2001-03-14 The Procter & Gamble Company Serinproteasevarianten mit substituierten aminosäuren
BR0012693A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante, de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
CN1399677A (zh) 1999-07-22 2003-02-26 宝洁公司 在确定表位区有氨基酸取代的枯草杆菌蛋白酶变体
CA2379729A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Protease conjugates having sterically protected clip sites
AU5911599A (en) * 1999-09-09 2001-04-10 Procter & Gamble Company, The A detergent composition containing a protease
US20050101012A1 (en) 2001-03-12 2005-05-12 Gerold Schuler CD4+CD25+ regulatory T cells from human blood
DE10153792A1 (de) * 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
EP1321513A1 (de) * 2001-12-21 2003-06-25 Direvo Biotech AG Subtilisin variante mit verbesserten Eigenschaften
US8222033B2 (en) 2002-08-12 2012-07-17 Argos Therapeutics, Inc. CD4+CD25− T cells and Tr1-like regulatory T cells
CN101454452B (zh) * 2003-08-06 2014-01-01 马里兰大学生物技术研究所 用于亲和纯化的工程蛋白酶及融合蛋白的加工
US20100233780A1 (en) 2007-06-06 2010-09-16 Wolfgang Aehle Methods for Improving Protein Properties
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
EP2578680B1 (de) * 2008-06-06 2016-04-27 Danisco US Inc. Zusammensetzungen und Verfahren mit mikrobiellen Proteasevarianten
EP2166076A1 (de) 2008-09-23 2010-03-24 The Procter & Gamble Company Reinigungszusammensetzung
WO2010056653A2 (en) 2008-11-11 2010-05-20 Danisco Us Inc. Proteases comprising one or more combinable mutations
EP2814956B1 (de) * 2012-02-17 2017-05-10 Novozymes A/S Subtilisinvarianten und polynukleotide zu deren codierung
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
JPH04500385A (ja) * 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
EP0563169B2 (de) * 1990-12-21 2006-04-12 Novozymes A/S Enzym-mutanten mit einer geringen variation der molekularladung über einen ph-bereich

Also Published As

Publication number Publication date
HUT74222A (en) 1996-11-28
JPH09502610A (ja) 1997-03-18
AU7870394A (en) 1995-04-03
CN1057123C (zh) 2000-10-04
WO1995007991A2 (en) 1995-03-23
CA2170491C (en) 2001-10-30
DE69434873T2 (de) 2007-09-06
CZ79196A3 (en) 1996-06-12
EP0719339A1 (de) 1996-07-03
CA2170491A1 (en) 1995-03-23
CN1134728A (zh) 1996-10-30
ES2275265T3 (es) 2007-06-01
NO961067L (no) 1996-05-15
BR9407502A (pt) 1997-01-07
MA23330A1 (fr) 1995-04-01
ATE343636T1 (de) 2006-11-15
WO1995007991A3 (en) 1995-04-13
HU9600667D0 (en) 1996-05-28
NO961067D0 (no) 1996-03-15
EP0719339B1 (de) 2006-10-25

Similar Documents

Publication Publication Date Title
DE69434873D1 (de) Subtilisin bpn'-varianten mit verminderter adsorption und erhöhter hydrolyse
MX9605359A (es) Composiciones para la limpieza de telas que contienen variantes de subtilicina bpn.
MX9605357A (es) Variantes de subtilisina bpn' que tiene absorcion reducida e hidrolisis incrementada.
MX9605358A (es) Variantes de subtilicina 309 con absorcion disminuida e hidrolisis aumentada.
MX9706851A (es) Variantes de subtilisina carlseberg que tienen adsorcion reducida e hidrolisis incrementada.
AU6424394A (en) Protease variants
AU8079794A (en) Protease-containing cleaning compositions
DK0723590T3 (da) Subtilisinvarianter
CA2160131A1 (en) New Bacillus Thuringiensis Strains and Their Insecticidal Proteins
MY114140A (en) Novel enzymes and prodrugs for use in antibody-directed enzyme prodrug therapy.
MX9706847A (es) Variantes de subtilisina dy que tienen adsorcion reducida e hidrolisis incrementada.
DE69623328D1 (de) Verwendung von mannanases als schleimkontrollmittel
HU9600297D0 (en) Rye-flour
TW288046B (en) Nucleotide sequence of cDNA of a thrombin-like enzyme (ancrod) from Calloselasma rhodostoma and amino acid sequence of ancrod therefrom
CO4290380A1 (es) Composiciones limpiadoras de tela que contienen proteasa de serina

Legal Events

Date Code Title Description
8364 No opposition during term of opposition