DE69430665D1 - Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits - Google Patents

Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits

Info

Publication number
DE69430665D1
DE69430665D1 DE69430665T DE69430665T DE69430665D1 DE 69430665 D1 DE69430665 D1 DE 69430665D1 DE 69430665 T DE69430665 T DE 69430665T DE 69430665 T DE69430665 T DE 69430665T DE 69430665 D1 DE69430665 D1 DE 69430665D1
Authority
DE
Germany
Prior art keywords
kits
nucleic acid
hybridization assay
sandwich hybridization
sensitive nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69430665T
Other languages
English (en)
Other versions
DE69430665T2 (de
Inventor
Sanjay Tyagi
Ulf D Landegran
Paul M Lizardi
Fred R Kramer
Herman J Blok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medicine And Dentistry Of Ne Us, University of
Original Assignee
Public Health Research Institute of City of New York Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Public Health Research Institute of City of New York Inc filed Critical Public Health Research Institute of City of New York Inc
Publication of DE69430665D1 publication Critical patent/DE69430665D1/de
Application granted granted Critical
Publication of DE69430665T2 publication Critical patent/DE69430665T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69430665T 1993-01-15 1994-01-14 Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits Expired - Lifetime DE69430665T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US607393A 1993-01-15 1993-01-15
US15736793A 1993-11-23 1993-11-23
PCT/US1994/000661 WO1994016108A1 (en) 1993-01-15 1994-01-14 Sensitive nucleic acid sandwich hybridization assays and kits

Publications (2)

Publication Number Publication Date
DE69430665D1 true DE69430665D1 (de) 2002-06-27
DE69430665T2 DE69430665T2 (de) 2002-11-21

Family

ID=26675135

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69430665T Expired - Lifetime DE69430665T2 (de) 1993-01-15 1994-01-14 Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits

Country Status (6)

Country Link
US (1) US5759773A (de)
EP (1) EP0688366B1 (de)
JP (1) JP3778925B2 (de)
AU (1) AU6031094A (de)
DE (1) DE69430665T2 (de)
WO (1) WO1994016108A1 (de)

Families Citing this family (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1994016105A1 (en) * 1993-01-15 1994-07-21 The Public Health Research Institute Of The City Of New York, Inc. Rna assays using rna binary probes and ribozyme ligase
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6207373B1 (en) 1998-02-25 2001-03-27 Nanogen, Inc. Methods for determining nature of repeat units in DNA
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
GB9415129D0 (en) * 1994-07-27 1994-09-14 Lynxvale Ltd Oligonucleotides and their use
SE504798C2 (sv) * 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6630947B1 (en) * 1996-04-10 2003-10-07 The United States Of America As Represented By The Secretary Of The Navy Method for examining subsurface environments
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
BE1011052A3 (fr) * 1997-03-20 1999-04-06 Jose Remacle Procede et trousse de diagnostic et/ou de quantification par hybridation de type sandwich de sequences d'acides nucleiques sur support solide.
US5837466A (en) * 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
AU5927598A (en) * 1997-01-23 1998-08-18 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries
US6037137A (en) * 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6194149B1 (en) * 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
CA2289925A1 (en) 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
US6037126A (en) * 1997-06-12 2000-03-14 Invitro Diagnostics, Inc. Compositions, methods, kits and apparatus for determining the presence or absence of protein component of telomerase enzyme
AU756301B2 (en) * 1997-08-20 2003-01-09 Somagenics, Inc. Antisense and antigene therapeutics with improved binding properties and methods for their use
JP2002505846A (ja) 1997-11-06 2002-02-26 モザイク テクノロジーズ シグナル増幅およびプロセッシングのための多重逐次的ポリヌクレオチド置換反応
BR9814272A (pt) * 1997-12-12 2000-10-03 Digene Corp Meio de coleta universal
CA2331767A1 (en) 1998-06-18 1999-12-23 Mosaic Technologies Denaturing gradient affinity electrophoresis and methods of use thereof
EP1092046A4 (de) * 1998-07-02 2003-02-19 Invitro Diagnostics Inc Verfahren, zusammensetzngen und apparat zur herstellung von nukleinsäuremolekülen mit selektiver affinität für ein zielmolekül
WO2000004193A1 (en) * 1998-07-20 2000-01-27 Yale University Method for detecting nucleic acids using target-mediated ligation of bipartite primers
FR2783253B1 (fr) * 1998-09-16 2002-03-01 Commissariat Energie Atomique Procede et trousse pour la detection d'un desequilibre intrachromosomique dans des noyaux interphasiques et leurs applications
US6238927B1 (en) 1998-10-05 2001-05-29 Mosaic Technologies, Incorporated Reverse displacement assay for detection of nucleic acid sequences
WO2000026412A1 (en) * 1998-11-02 2000-05-11 Kenneth Loren Beattie Nucleic acid analysis using sequence-targeted tandem hybridization
EP1422298B1 (de) * 1999-07-09 2010-01-27 Gen-Probe Incorporated HIV-1 Detektion mittels Nukleinsäureamplifizierung
US20010031467A1 (en) * 1999-12-10 2001-10-18 Johannes Dapprich Method for selectively isolating a nucleic acid
ES2253279T3 (es) * 1999-12-15 2006-06-01 Gen-Probe Incorporated Metodos y composiciones para la deteccion de especies del complejo de mycobacterium avium.
US6808876B1 (en) * 2000-05-02 2004-10-26 Immusol, Inc. Cellular regulators of infectious agents and methods of use
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US6562575B1 (en) * 2000-06-26 2003-05-13 Epicentre Technologies Corporation Analyte-specific assays based on formation of a replicase substrate
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
AU2003303395A1 (en) * 2001-05-22 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
US8152991B2 (en) 2005-10-27 2012-04-10 Nanomix, Inc. Ammonia nanosensors, and environmental control system
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
SE0200531D0 (sv) * 2002-02-22 2002-02-22 Avaris Ab Novel biomolecular complexes, method for their production and use
AU2003230619A1 (en) * 2002-03-08 2003-09-22 Naxcor Compositions and methods for detecting nucleic acid methylation
US7714398B2 (en) * 2002-09-05 2010-05-11 Nanomix, Inc. Nanoelectronic measurement system for physiologic gases and improved nanosensor for carbon dioxide
US20050129573A1 (en) * 2003-09-12 2005-06-16 Nanomix, Inc. Carbon dioxide nanoelectronic sensor
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US20050026161A1 (en) * 2002-11-01 2005-02-03 Edward Jablonski Displacement sandwich immuno-PCR
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
DE102004023439B4 (de) * 2004-05-12 2006-03-16 Eppendorf Ag Nachweis von RNA über Micro-Arrays
EP1756307A1 (de) * 2004-05-20 2007-02-28 Trillion Genomics Limited Verwendung massenmarkierter sonden zum nachweis von zielnukleinsäuren mittels massenspektrometrie
WO2006020617A1 (en) * 2004-08-09 2006-02-23 Generation Biotech, Llc Method for nucleic acid isolation and amplification
DE602005023529D1 (de) * 2004-11-03 2010-10-21 Iris Molecular Diagnostics Inc Homogener nachweis von analyten
WO2006137933A2 (en) * 2004-11-03 2006-12-28 Leucadia Technologies, Inc. Microbubbles for affinity separation
US8669052B2 (en) 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
WO2007114931A2 (en) * 2006-04-04 2007-10-11 Nanomix, Inc. Nanoelectronic detection of biomolecules employing analyte amplification and reporters
US8975215B2 (en) * 2007-09-17 2015-03-10 Postech Foundation Methods for producing surface bound oligonucleotide on solid substrate and uses thereof
WO2009092035A2 (en) * 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
EP2252893B1 (de) * 2008-02-21 2013-10-23 Iris International, Inc. Verfahren zur frühen bestimmung des wiederauftretens nach einer therapie gegen prostatakrebs
JP2011518333A (ja) * 2008-04-17 2011-06-23 キアジェン ゲイサーズバーグ インコーポレイテッド 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
EP2313527A4 (de) * 2008-07-15 2012-11-21 Rapid Pathogen Screening Inc Lateralfluss-nukleinsäuredetektor
US8288520B2 (en) 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
JP6108661B2 (ja) * 2009-01-28 2017-04-05 キアジェン ゲイサーズバーグ インコーポレイテッド 配列特異的な大量試料調製方法およびアッセイ法
JP5419138B2 (ja) * 2009-03-27 2014-02-19 Ｎｅｃソフト株式会社 検出対象物の検出方法およびそれに用いる検出用キット
CA2760542A1 (en) * 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
US8993346B2 (en) 2009-08-07 2015-03-31 Nanomix, Inc. Magnetic carbon nanotube based biodetection
EP2478087B1 (de) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Zusammensetzungen und verfahren zur rückgewinnung von nukleinsäuren oder proteinen aus in zytologischen medien fixierten gewebeproben
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
WO2011094514A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
DK2576577T3 (en) 2010-05-28 2015-04-20 Life Technologies Corp Synthesis of 2 ', 3'-dideoxynucleosides for automated DNA synthesis and PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION
WO2012018964A1 (en) 2010-08-03 2012-02-09 Life Technologies Corporation Detection of salmonella enterica subspecies enterica serovar enteritidis in food and environmental samples, methods and compositions thereof
EP2616555B1 (de) 2010-09-16 2017-11-08 Gen-Probe Incorporated Über l-nukleotidenden immobilisierbare fängersonden
US20120208189A1 (en) 2011-01-14 2012-08-16 Life Technologies Corporation Methods for isolation, identification, and quantification of mirnas
DK3216878T3 (da) 2011-01-17 2019-06-11 Life Technologies Corp Arbejdsprocedure til påvisning af ligander ved anvendelse af nukleinsyrer
CA2828224A1 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acids
WO2012159025A2 (en) 2011-05-18 2012-11-22 Life Technologies Corporation Chromosome conformation analysis
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP2718260B1 (de) 2011-06-08 2018-07-25 Life Technologies Corporation Design und entwicklung neuer reinigungsmittel zur verwendung in pcr-systemen
US9212386B2 (en) 2011-07-22 2015-12-15 Rapid Pathogen Screening, Inc. Enzymatic cleavage based lateral flow assays
EP2861757B1 (de) 2012-06-14 2019-11-06 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits für polymerasekettenreaktionen (pcr)
EP2914618B1 (de) 2012-11-02 2017-07-26 Novartis Tiergesundheit AG Mit 'theiler's disease' assoziiertes flavivirus
US9920360B2 (en) 2012-11-02 2018-03-20 Life Technologies Corporation Small RNA capture, detection and quantification
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
CA2904181A1 (en) 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
US20160024565A1 (en) * 2013-03-13 2016-01-28 Tufts University Fragment complementation of based assays
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
EP3063129B1 (de) 2013-10-25 2019-04-17 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
US10752940B2 (en) * 2013-11-08 2020-08-25 Ionis Pharmaceuticals, Inc. Compounds and methods for detecting oligonucleotides
WO2015073080A1 (en) 2013-11-12 2015-05-21 Life Technologies Corporation Reagents and methods for sequencing
EP3143401A4 (de) 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Verbesserte testverfahren
US20170253921A1 (en) 2014-10-13 2017-09-07 Life Technologies Corporation Methods, kits & compositions for determining gene copy numbers
EP3967768A1 (de) 2015-03-13 2022-03-16 Life Technologies Corporation Zusammensetzungen für erfassung, nachweis und quantifizierung von kleinen rna-molekülen
CN107532219B (zh) 2015-03-25 2023-05-02 安戈欧洲有限公司 使用链置换聚合酶的固相靶核酸捕获和复制
WO2017177025A1 (en) 2016-04-06 2017-10-12 Life Technologies Corporation Compositions, methods, and kits for synthesis and detection of nucleic acids
WO2017192737A1 (en) * 2016-05-03 2017-11-09 The Regents Of The University Of California Integrated electrochemical detection and purification of nucleic acid biomarkers
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3472349A1 (de) 2016-06-16 2019-04-24 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits zum mikroorganismusnachweis
CN109689886A (zh) 2016-08-26 2019-04-26 生命技术公司 核酸提取和扩增对照及其使用方法
US11572591B2 (en) 2017-04-26 2023-02-07 The Translational Genomics Research Institute Methods and assays for subtyping Staphylococcus aureus clonal complex 8 strains
JP2021502825A (ja) 2017-11-13 2021-02-04 ライフ テクノロジーズ コーポレイション 尿路微生物検出のための組成物、方法、およびキット
CA3186950A1 (en) 2020-07-23 2022-01-27 Scott Benson Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same
KR20230056683A (ko) 2020-07-23 2023-04-27 라이프 테크놀로지스 코포레이션 생물학적 분석에 사용하기 위한 에너지 전달 염료 접합체

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0365627B1 (de) * 1988-03-24 1993-12-22 University Of Iowa Research Foundation Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird
WO1990001069A1 (en) * 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
EP0439182B1 (de) * 1990-01-26 1996-04-24 Abbott Laboratories Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
WO1992018521A1 (en) * 1991-04-10 1992-10-29 Life Technologies, Inc. Method for amplifying and altering an rna sequence
DE69434688T2 (de) * 1993-01-15 2007-01-11 The Public Health Research Institute Of The City Of New York, Inc. Diagnostischer nachweis von rna und reagentiensätze unter verwendung binärer rna proben und einer rna-abhängigen rna ligase

Also Published As

Publication number Publication date
JPH08505770A (ja) 1996-06-25
JP3778925B2 (ja) 2006-05-24
WO1994016108A1 (en) 1994-07-21
EP0688366B1 (de) 2002-05-22
AU6031094A (en) 1994-08-15
DE69430665T2 (de) 2002-11-21
US5759773A (en) 1998-06-02
EP0688366A1 (de) 1995-12-27
EP0688366A4 (de) 1999-04-21

Similar Documents

Publication Publication Date Title
DE69430665T2 (de) Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits
AU581577B2 (en) Nucleic acid hybridization assay and detectable molecules useful in such assay
DE69840331D1 (de) Polyvalente und polyspezifische reagentienmit hoher avidität
FI960469A0 (fi) DNA:ta sekvenssoivia entsyymejä
DE69635744D1 (de) Modifizierte Nukleinsäuresonden
DE69431719T2 (de) Hybridisierung und sequenzierung von nukleinsäuren
DE69328699T2 (de) Hybridisierungsassay unter Verwendung von verzweigten Nukleinsäuresonden
DE69636843D1 (de) Nachweis von Nukleinsäure-Änderungen
DE69738687D1 (de) Sonden, kits und assays
IL85551A0 (en) Rapid hybridization assay and reagent system used therein
DK0587331T3 (da) Nukleinsyreprober til Chlamydia pneumoniae.
DK0572120T3 (da) Nucleinsyre-probertil mycobacterium tuberculosis
DE69331844T2 (de) Assay für die chagas-krankheit und reagenzien zu seiner verwendung
DE69801266D1 (de) Diazoniumionen enthaltende Reagentien und ihre Verwendung
DE69421398T2 (de) Markierte kohlenhydrate und ihre verwendung in assays
BR9404246A (pt) Genes de aglutinação de levedura e levedura contendo os mesmos
GB9417793D0 (en) Bioluminescence and assay reagents
HU9800782D0 (en) Novel antibody, renin-active substance containing the same and prorenin assay reagent using the same
PT977868E (pt) Estirpes de levedura contendo o gene atf2 interrompido e suas aplicacoes
AU7541894A (en) Cell reagent and assay
DE69307932D1 (de) Dideoxyfructonukleotide und deoxyfructonukleotide als dna polymerase-substrate
GB9417798D0 (en) Bioluminescence and assay reagents
GB9317744D0 (en) Bioluminescence and assay reagents
GB9317812D0 (en) Bioluminescence and assay reagents
GB9218013D0 (en) Bioluminescence and assay reagents

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: THE UNIVERSITY OF MEDICINE AND DENTISTRY OF NE, US