DE69333342D1 - Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase - Google Patents

Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase

Info

Publication number
DE69333342D1
DE69333342D1 DE69333342T DE69333342T DE69333342D1 DE 69333342 D1 DE69333342 D1 DE 69333342D1 DE 69333342 T DE69333342 T DE 69333342T DE 69333342 T DE69333342 T DE 69333342T DE 69333342 D1 DE69333342 D1 DE 69333342D1
Authority
DE
Germany
Prior art keywords
cytochrome
eukaryotic
reductase
fusion proteins
fusion protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69333342T
Other languages
English (en)
Inventor
W Fisher
J Barnes
W Estabrook
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System filed Critical University of Texas System
Application granted granted Critical
Publication of DE69333342D1 publication Critical patent/DE69333342D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0085—Steroid 17 alpha-monooxygenase (1.14.99.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99009—Steroid 17-alpha-monooxygenase (1.14.99.9), i.e. cytochrome-P450-steroid-17-alpha-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
DE69333342T 1992-07-02 1993-06-29 Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase Expired - Lifetime DE69333342D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/908,317 US5420027A (en) 1991-01-10 1992-07-02 Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria
PCT/US1993/006171 WO1994001568A1 (en) 1992-07-02 1993-06-29 Fusion proteins comprising eukaryotic cytochrome p450 fused to a reductase

Publications (1)

Publication Number Publication Date
DE69333342D1 true DE69333342D1 (de) 2004-01-22

Family

ID=25425584

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69333342T Expired - Lifetime DE69333342D1 (de) 1992-07-02 1993-06-29 Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase

Country Status (5)

Country Link
US (1) US5420027A (de)
EP (1) EP0649472B1 (de)
AT (1) ATE256190T1 (de)
DE (1) DE69333342D1 (de)
WO (1) WO1994001568A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3315827B2 (ja) * 1994-10-07 2002-08-19 オリエンタル酵母工業株式会社 活性型ヒトａｌｔの製造方法
GB9615032D0 (en) 1996-07-17 1996-09-04 Univ Dundee Enzyme system
US9255256B2 (en) * 1996-07-17 2016-02-09 Btg International Limited Expression of functional cytochorome P450 monooxygenase system in enterobacteria
US6492316B1 (en) * 1997-07-09 2002-12-10 The Procter & Gamble Company Cleaning compositions comprising a cytochrome
US6432639B1 (en) * 1997-09-10 2002-08-13 Dna Sciences Laboratories, Inc. Isolated CYP3A4 nucleic acid molecules and detection methods
WO2000006747A2 (en) * 1998-07-30 2000-02-10 Metabolix, Inc. Enzymes for biopolymer production
US6929912B2 (en) * 1998-08-31 2005-08-16 Genaissance Pharmaceuticals, Inc. Methods for evaluating the ability to metabolize pharmaceuticals
JP2002233378A (ja) * 2001-02-07 2002-08-20 Nihon Nosan Kogyo Kk ヒト由来のチトクロムｐ４５０分子種を認識する抗体
GB0119366D0 (en) * 2001-08-08 2001-10-03 Imperial College Enzymes and enzymic processes
EP2298354B1 (de) 2001-10-10 2014-03-19 ratiopharm GmbH Neumodulierung und Glykokonjugation von Interferon-beta
EP2292271A3 (de) 2001-10-10 2011-09-14 BioGeneriX AG Neumodulierung und Glykokonjugation von Antikörpern
PL1615945T3 (pl) 2003-04-09 2012-03-30 Ratiopharm Gmbh Sposoby glikopegylacji i białka/peptydy wytwarzane tymi sposobami
KR100665316B1 (ko) 2005-06-03 2007-01-09 한국생명공학연구원 메타게놈 유래의 신규한 이중 활성 수산화효소, 이를코드하는 유전자 및 그 스크리닝 방법
TWI407264B (zh) * 2009-03-10 2013-09-01 Nat Applied Res Laboratoires Microcomputer and its application
CN112725296B (zh) * 2021-01-28 2023-06-13 江南大学 一种黄体酮-17α-羟化酶及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766068A (en) * 1984-06-16 1988-08-23 Agency Of Industrial Science And Technology Cytochrome P-450MC gene, expression plasmid carrying the said gene, yeasts transformed with the said plasmid and a process for producing cytochrome P-450MC by culturing the said transformant yeasts
US4792523A (en) * 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
US5045471A (en) * 1986-12-02 1991-09-03 The Regents Of The University Of California Cloned DNA for P450scc and expression thereof
PT90484B (pt) * 1988-05-06 1994-08-31 Roussel Uclaf Processo para a oxidacao bioquimica de esteroides e para a preparacao por engenharia genetica de celulas para esse fim
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
US5073627A (en) * 1989-08-22 1991-12-17 Immunex Corporation Fusion proteins comprising GM-CSF and IL-3
US5212296A (en) * 1989-09-11 1993-05-18 E. I. Du Pont De Nemours And Company Expression of herbicide metabolizing cytochromes
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria

Also Published As

Publication number Publication date
WO1994001568A1 (en) 1994-01-20
ATE256190T1 (de) 2003-12-15
EP0649472A1 (de) 1995-04-26
US5420027A (en) 1995-05-30
EP0649472A4 (de) 1997-03-19
EP0649472B1 (de) 2003-12-10

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