ATE256190T1 - Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase - Google Patents

Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase

Info

Publication number
ATE256190T1
ATE256190T1 AT93916837T AT93916837T ATE256190T1 AT E256190 T1 ATE256190 T1 AT E256190T1 AT 93916837 T AT93916837 T AT 93916837T AT 93916837 T AT93916837 T AT 93916837T AT E256190 T1 ATE256190 T1 AT E256190T1
Authority
AT
Austria
Prior art keywords
cytochrome
eukaryotic
reductase
fusion proteins
coupled
Prior art date
Application number
AT93916837T
Other languages
English (en)
Inventor
Charles W Fisher
Henry J Barnes
Ronald W Estabrook
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Application granted granted Critical
Publication of ATE256190T1 publication Critical patent/ATE256190T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0085—Steroid 17 alpha-monooxygenase (1.14.99.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99009—Steroid 17-alpha-monooxygenase (1.14.99.9), i.e. cytochrome-P450-steroid-17-alpha-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT93916837T 1992-07-02 1993-06-29 Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase ATE256190T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/908,317 US5420027A (en) 1991-01-10 1992-07-02 Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria
PCT/US1993/006171 WO1994001568A1 (en) 1992-07-02 1993-06-29 Fusion proteins comprising eukaryotic cytochrome p450 fused to a reductase

Publications (1)

Publication Number Publication Date
ATE256190T1 true ATE256190T1 (de) 2003-12-15

Family

ID=25425584

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93916837T ATE256190T1 (de) 1992-07-02 1993-06-29 Fusionsprotein aus eukaryotischem cytochrom p450 gekoppelt an eine reduktase

Country Status (5)

Country Link
US (1) US5420027A (de)
EP (1) EP0649472B1 (de)
AT (1) ATE256190T1 (de)
DE (1) DE69333342D1 (de)
WO (1) WO1994001568A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3315827B2 (ja) * 1994-10-07 2002-08-19 オリエンタル酵母工業株式会社 活性型ヒトａｌｔの製造方法
US9255256B2 (en) * 1996-07-17 2016-02-09 Btg International Limited Expression of functional cytochorome P450 monooxygenase system in enterobacteria
GB9615032D0 (en) 1996-07-17 1996-09-04 Univ Dundee Enzyme system
US6492316B1 (en) * 1997-07-09 2002-12-10 The Procter & Gamble Company Cleaning compositions comprising a cytochrome
US6432639B1 (en) * 1997-09-10 2002-08-13 Dna Sciences Laboratories, Inc. Isolated CYP3A4 nucleic acid molecules and detection methods
DE69929700T2 (de) * 1998-07-30 2006-08-31 Metabolix, Inc., Cambridge Bifunktionelle enzyme zur biopolymerherstellung
US6929912B2 (en) * 1998-08-31 2005-08-16 Genaissance Pharmaceuticals, Inc. Methods for evaluating the ability to metabolize pharmaceuticals
JP2002233378A (ja) * 2001-02-07 2002-08-20 Nihon Nosan Kogyo Kk ヒト由来のチトクロムｐ４５０分子種を認識する抗体
GB0119366D0 (en) * 2001-08-08 2001-10-03 Imperial College Enzymes and enzymic processes
EP2305312B1 (de) 2001-10-10 2015-03-04 ratiopharm GmbH Neumodulierung und Glykokonjugation von Follitropin (FSH)
ES2561985T3 (es) 2001-10-10 2016-03-01 Ratiopharm Gmbh Remodelación y glicoconjugación de anticuerpos
EP2338333B1 (de) 2003-04-09 2017-09-06 ratiopharm GmbH Glycopegylierungsverfahren und durch die Verfahren hergestellte Proteine/Peptide
KR100665316B1 (ko) 2005-06-03 2007-01-09 한국생명공학연구원 메타게놈 유래의 신규한 이중 활성 수산화효소, 이를코드하는 유전자 및 그 스크리닝 방법
TWI407264B (zh) * 2009-03-10 2013-09-01 Nat Applied Res Laboratoires Microcomputer and its application
CN112725296B (zh) * 2021-01-28 2023-06-13 江南大学 一种黄体酮-17α-羟化酶及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766068A (en) * 1984-06-16 1988-08-23 Agency Of Industrial Science And Technology Cytochrome P-450MC gene, expression plasmid carrying the said gene, yeasts transformed with the said plasmid and a process for producing cytochrome P-450MC by culturing the said transformant yeasts
US4792523A (en) * 1984-08-31 1988-12-20 Cetus Corporation 3'Expression enhancing fragments and method
US5045471A (en) * 1986-12-02 1991-09-03 The Regents Of The University Of California Cloned DNA for P450scc and expression thereof
PT90484B (pt) * 1988-05-06 1994-08-31 Roussel Uclaf Processo para a oxidacao bioquimica de esteroides e para a preparacao por engenharia genetica de celulas para esse fim
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
US5073627A (en) * 1989-08-22 1991-12-17 Immunex Corporation Fusion proteins comprising GM-CSF and IL-3
US5212296A (en) * 1989-09-11 1993-05-18 E. I. Du Pont De Nemours And Company Expression of herbicide metabolizing cytochromes
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria

Also Published As

Publication number Publication date
EP0649472A1 (de) 1995-04-26
DE69333342D1 (de) 2004-01-22
EP0649472A4 (de) 1997-03-19
WO1994001568A1 (en) 1994-01-20
EP0649472B1 (de) 2003-12-10
US5420027A (en) 1995-05-30

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