DE69229174D1 - Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen - Google Patents

Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen

Info

Publication number
DE69229174D1
DE69229174D1 DE69229174T DE69229174T DE69229174D1 DE 69229174 D1 DE69229174 D1 DE 69229174D1 DE 69229174 T DE69229174 T DE 69229174T DE 69229174 T DE69229174 T DE 69229174T DE 69229174 D1 DE69229174 D1 DE 69229174D1
Authority
DE
Germany
Prior art keywords
cells
reporter
block
substrate
haloalkyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69229174T
Other languages
English (en)
Other versions
DE69229174T2 (de
Inventor
Richard Haugland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Probes Inc
Original Assignee
Molecular Probes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Probes Inc filed Critical Molecular Probes Inc
Application granted granted Critical
Publication of DE69229174D1 publication Critical patent/DE69229174D1/de
Publication of DE69229174T2 publication Critical patent/DE69229174T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/40—Triphenylmethane dye chromogens, e.g. fluorescein derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/20—Coumarin derivatives
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/802—Chromogenic or luminescent peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69229174T 1991-08-23 1992-08-21 Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen Expired - Lifetime DE69229174T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74925691A 1991-08-23 1991-08-23
PCT/US1992/007068 WO1993004192A1 (en) 1991-08-23 1992-08-21 Use of haloalkyl derivatives of reporter molecules to analyze metabolic activity in cells

Publications (2)

Publication Number Publication Date
DE69229174D1 true DE69229174D1 (de) 1999-06-17
DE69229174T2 DE69229174T2 (de) 1999-09-16

Family

ID=25012967

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69229174T Expired - Lifetime DE69229174T2 (de) 1991-08-23 1992-08-21 Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen

Country Status (5)

Country Link
US (1) US5362628A (de)
EP (1) EP0603266B1 (de)
AT (1) ATE180018T1 (de)
DE (1) DE69229174T2 (de)
WO (1) WO1993004192A1 (de)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5576424A (en) * 1991-08-23 1996-11-19 Molecular Probes, Inc. Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells
USRE38723E1 (en) * 1991-08-23 2005-04-12 Molecular Probes, Inc. Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells
US5352803A (en) * 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives
CA2131727A1 (en) * 1992-03-30 1993-10-14 Diana E. Clarisse Reagents and methods for the detection and quantification of thyroxine in fluid samples
US5534416A (en) * 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US6187951B1 (en) 1993-06-29 2001-02-13 Cargill, Incorporated Lactic acid production, separation and/or recovery process
US5510526A (en) * 1993-06-29 1996-04-23 Cargill, Incorporated Lactic acid production, separation and/or recovery process
US5459268A (en) * 1993-10-25 1995-10-17 Molecular Probes, Inc. Xanthylium dyes that are well retained in mitochondria
US5798276A (en) * 1995-06-07 1998-08-25 Molecular Probes, Inc. Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US5830912A (en) * 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US5773236A (en) * 1997-04-25 1998-06-30 Molecule Probes, Inc. Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules
US6342611B1 (en) 1997-10-10 2002-01-29 Cytovia, Inc. Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for capsases and other enzymes and the use thereof
FR2781055B1 (fr) * 1998-07-09 2000-10-13 Immunotech Sa Reactif et methode pour la permeabilisation et l'identification des erythrocytes
EP1100520B1 (de) * 1998-07-21 2004-09-22 Cytovia, Inc. Neue fluoreszensfarbstoffe und ihre anwendung in fluoreszensnachweisverfahren ganzer zellen für kaspasen, peptidasen, proteasen und andere enzyme sowie deren verwendung
US6770468B1 (en) 1999-09-14 2004-08-03 Genzyme Glycobiology Research Institute, Inc. Phosphodiester-α-GlcNAcase of the lysosomal targeting pathway
US6534300B1 (en) 1999-09-14 2003-03-18 Genzyme Glycobiology Research Institute, Inc. Methods for producing highly phosphorylated lysosomal hydrolases
AU2001278135A1 (en) 2000-08-03 2002-02-18 Cytovia, Inc. Method of identifying immunosuppressive agents
AU2002243541A1 (en) * 2001-06-01 2002-12-16 Cytovia, Inc. Methods of identifying anti-cancer agents that are inducers of apoptosis
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US20050069962A1 (en) * 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
US6800472B2 (en) 2001-12-21 2004-10-05 Genzyme Glycobiology Research Institute, Inc. Expression of lysosomal hydrolase in cells expressing pro-N-acetylglucosamine-1-phosphodiester α-N-acetyl glucosimanidase
US6905856B2 (en) 2001-12-21 2005-06-14 Genzyme Glycobiology Research Institute, Inc. Soluble GlcNAc phosphotransferase
US20040171034A1 (en) 2002-05-03 2004-09-02 Brian Agnew Compositions and methods for detection and isolation of phosphorylated molecules
US7445894B2 (en) * 2002-05-03 2008-11-04 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
CN1665790A (zh) * 2002-05-03 2005-09-07 莫莱丘莱尔探针公司 用于检测和分离磷酸化分子的组合物及方法
WO2004025259A2 (en) 2002-09-12 2004-03-25 Molecular Probes, Inc. Site-specific labeling of affinity tags in fusion proteins
US20040106163A1 (en) * 2002-11-12 2004-06-03 Workman Jerome James Non-invasive measurement of analytes
WO2004044557A2 (en) * 2002-11-12 2004-05-27 Argose, Inc. Non-invasive measurement of analytes
EP2341134B1 (de) 2003-01-31 2014-08-27 Promega Corporation Kovalentes Anbinden von Funktionsgruppen an Proteine
US20050250214A1 (en) * 2004-05-05 2005-11-10 Gee Kyle R Zinc binding compounds and their method of use
US8445702B2 (en) 2003-05-05 2013-05-21 Life Technologies Corporation Zinc binding compounds and their method of use
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US20050074796A1 (en) * 2003-07-31 2005-04-07 Stephen Yue Unsymmetrical cyanine dimer compounds and their application
US7271265B2 (en) * 2003-08-11 2007-09-18 Invitrogen Corporation Cyanine compounds and their application as quenching compounds
WO2005042504A1 (en) 2003-10-31 2005-05-12 Molecular Probes, Inc. Fluorinated resorufin compounds and their application in detecting hydrogen peroxide
US20050214807A1 (en) * 2003-11-19 2005-09-29 Iain Johnson Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection
US7446202B2 (en) 2003-12-05 2008-11-04 Molecular Probes, Inc. Cyanine dye compounds
WO2005056687A2 (en) 2003-12-05 2005-06-23 Molecular Probes, Inc. Methine-substituted cyanine dye compounds
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US8039642B2 (en) 2003-12-09 2011-10-18 Life Technologies Corporation Pyrenyloxysulfonic acid fluorescent agents
WO2005064336A1 (en) * 2003-12-09 2005-07-14 Molecular Probes, Inc. Pyrenyloxysulfonic acid fluorescent agents
WO2005065241A2 (en) * 2003-12-24 2005-07-21 Argose, Inc. Smmr (small molecule metabolite reporters) for use as in vivo glucose biosensors
EP1711504A2 (de) 2004-01-21 2006-10-18 Molecular Probes Inc. Derivate von cephalosporin und clavulansäure zum nachweis von beta-lactamase in einer probe
WO2006023231A2 (en) 2004-07-27 2006-03-02 Molecular Probes, Inc. Fluorescent metal ion indicators with large stokes shift
WO2006124816A1 (en) 2005-05-11 2006-11-23 Molecular Probes, Inc. Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use
US20070134685A1 (en) * 2005-09-06 2007-06-14 Invitrogen Corporation Control of chemical modification
US8158801B2 (en) * 2005-09-26 2012-04-17 Life Technologies Corporation Violet laser excitable dyes and their method of use
US8093012B2 (en) * 2005-10-13 2012-01-10 Aureon Laboratories, Inc. Multiplex in situ immunohistochemical analysis
US7842823B2 (en) * 2005-10-27 2010-11-30 The Regents Of The University Of California Fluorogenic probes for reactive oxygen species
US7521577B2 (en) * 2006-01-12 2009-04-21 Molecular Probes, Inc. Heavy metal binding compounds and their method of use
US8551408B2 (en) 2006-01-24 2013-10-08 Life Technologies Corporation Device and methods for quantifying analytes
WO2007090126A2 (en) * 2006-01-30 2007-08-09 Invitrogen Corporation Compositions and methods for detecting and quantifying toxic substances in disease states
US8114636B2 (en) 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
EP4092418A1 (de) 2006-02-10 2022-11-23 Life Technologies Corporation Modifizierung von oligosacchariden und etikettierung von proteinen
WO2008076524A2 (en) 2006-10-27 2008-06-26 Life Technologies Corporation Fluorogenic ph sensitive dyes and their method of use
US8586743B2 (en) 2007-01-30 2013-11-19 Life Technologies Corporation Labeling reagents and methods of their use
WO2009018112A2 (en) * 2007-07-27 2009-02-05 Invitrogen Corporation The use of novel coumarins as glutathione and thiol labels
JP2010536347A (ja) 2007-08-17 2010-12-02 ユニヴァーシティ オブ ワシントン α−イズロニダーゼ酵素活性の検定のための方法
US20090247984A1 (en) * 2007-10-24 2009-10-01 Masimo Laboratories, Inc. Use of microneedles for small molecule metabolite reporter delivery
US8772487B2 (en) * 2008-01-24 2014-07-08 Life Technologies Corporation Fluorogenic hydrazine-substituted compounds
US8791258B2 (en) * 2008-06-10 2014-07-29 The Regents Of The University Of California Pro-fluorescent probes
WO2012022734A2 (en) 2010-08-16 2012-02-23 Medimmune Limited Anti-icam-1 antibodies and methods of use
EP3517956B1 (de) 2011-05-18 2021-09-08 Takeda Pharmaceutical Company Limited Modifikationsabhängige aktivitätstests
US9409928B2 (en) 2011-10-11 2016-08-09 The Hong Kong University Of Science And Technology Aggregation induced emission active cytophilic fluorescent bioprobes for long-term cell tracking
US9618453B2 (en) 2011-10-11 2017-04-11 The Hong Kong University Of Science And Technology Aggregation induced emission of fluorescent bioprobes and methods of using the same
US9512463B2 (en) 2011-11-08 2016-12-06 University Of Washington Methods and compositions for assaying the activity of one or more lysosomal enzymes
CN104470991A (zh) 2012-05-30 2015-03-25 生命科技公司 荧光pH敏感性染料和其使用方法
WO2015050959A1 (en) 2013-10-01 2015-04-09 Yale University Anti-kit antibodies and methods of use thereof
CN105848722B (zh) 2013-10-02 2021-09-03 免疫医疗有限责任公司 中和抗甲型流感抗体及其用途
WO2015175873A1 (en) 2014-05-16 2015-11-19 Life Technologies Corporation Cell tracking reagents and their methods of use
EP3143022B1 (de) 2014-05-16 2021-12-22 Life Technologies Corporation Als diagnostische hilfsmittel geeignete carbopyrononverbindungen
US10246410B2 (en) 2014-12-03 2019-04-02 Life Technologies Corporation Hydrazinyl and aminooxy compounds and their methods of use
US20180196033A1 (en) 2015-06-26 2018-07-12 Life Technologies Corporation Cell tracking reagents and their methods of use
EP3436012B1 (de) * 2016-03-30 2023-09-20 ConvaTec Technologies Inc. Modifizierte wundauflagen
EP3443126B1 (de) 2016-04-15 2023-11-15 Icahn School of Medicine at Mount Sinai Gewebeprofilierung unter verwendung von multiplexierten immunhistochemischen aufeinanderfolgenden färbungen
US10941435B2 (en) 2016-06-23 2021-03-09 Life Technologies Corporation Methods and compositions for detecting or measuring caspases or apoptosis
EP3494226B1 (de) 2016-08-03 2021-09-22 Lonza Walkersville, Inc. Verfahren zur detektion von endotoxin unter verwendung von limulus-amoebocyt-lysat, das im wesentlichen frei von coagulogen ist
WO2018132562A1 (en) 2017-01-11 2018-07-19 Lonza Walkersville, Inc. Coagulogen-free clarified limulus amebocyte lysate
AU2019319901B2 (en) 2018-08-10 2022-07-07 Life Technologies Corporation Silicon-substituted rhodamine dyes and dye conjugates
CN117836425A (zh) 2021-08-20 2024-04-05 武田药品工业株式会社 人因子viii的选择性测量

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE788200A (fr) * 1971-09-03 1973-02-28 Lonza Ag Procede de fabrication de 4-halogenomethylcoumarines et nouveaux composes ainsi obtenus
US4220722A (en) * 1978-02-10 1980-09-02 Syva Company Method for conjugating to polyamino compounds employing haloacyl groups and compositions prepared thereby
US4279992A (en) * 1978-03-13 1981-07-21 Miles Laboratories, Inc. Specific binding assay employing an enzyme-cleavable substrate as label
US4709016A (en) * 1982-02-01 1987-11-24 Northeastern University Molecular analytical release tags and their use in chemical analysis
US4803162A (en) * 1984-05-15 1989-02-07 Fluorodiagnostic Limited Partners Composition, article and process for detecting a microorganism
US4812409A (en) * 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
DE3614647A1 (de) * 1986-04-30 1987-11-05 Euratom 7-phenylessigsaeure-4-alkyl-coumarinylamide, verfahren zu ihrer herstellung sowie ihre verwendung in verfahren zur fluorometrischen bestimmung der aktivitaet von hydrolasen, insbesondere von penicillin-g-acylase
US5112739A (en) * 1988-08-02 1992-05-12 Polaroid Corporation Enzyme controlled release system

Also Published As

Publication number Publication date
EP0603266A1 (de) 1994-06-29
WO1993004192A1 (en) 1993-03-04
EP0603266A4 (en) 1997-01-15
DE69229174T2 (de) 1999-09-16
ATE180018T1 (de) 1999-05-15
EP0603266B1 (de) 1999-05-12
US5362628A (en) 1994-11-08

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