DE69225332D1 - Präparation von reinkulturen von humanen post-mitotischen neuronen - Google Patents
Präparation von reinkulturen von humanen post-mitotischen neuronenInfo
- Publication number
- DE69225332D1 DE69225332D1 DE69225332T DE69225332T DE69225332D1 DE 69225332 D1 DE69225332 D1 DE 69225332D1 DE 69225332 T DE69225332 T DE 69225332T DE 69225332 T DE69225332 T DE 69225332T DE 69225332 D1 DE69225332 D1 DE 69225332D1
- Authority
- DE
- Germany
- Prior art keywords
- cells
- pure
- preparation
- mitotic
- cultures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
- B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
- B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
- B29C41/12—Spreading-out the material on a substrate, e.g. on the surface of a liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
- B29C41/34—Component parts, details or accessories; Auxiliary operations
- B29C41/36—Feeding the material on to the mould, core or other substrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
- B29L2023/00—Tubular articles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/30—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cancer cells, e.g. reversion of tumour cells
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mechanical Engineering (AREA)
- Neurology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Neurosurgery (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/780,715 US5175103A (en) | 1991-10-21 | 1991-10-21 | Preparation of pure cultures of post-mitotic human neurons |
PCT/US1992/006540 WO1993008266A1 (en) | 1991-10-21 | 1992-08-05 | Preparation of pure cultures of post-mitotic human neurons |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69225332D1 true DE69225332D1 (de) | 1998-06-04 |
DE69225332T2 DE69225332T2 (de) | 1998-08-27 |
Family
ID=25120453
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69225332T Expired - Fee Related DE69225332T2 (de) | 1991-10-21 | 1992-08-05 | Präparation von reinkulturen von humanen post-mitotischen neuronen |
Country Status (9)
Country | Link |
---|---|
US (2) | US5175103A (de) |
EP (1) | EP0620847B1 (de) |
JP (1) | JP3169611B2 (de) |
AT (1) | ATE165617T1 (de) |
CA (1) | CA2120730A1 (de) |
DE (1) | DE69225332T2 (de) |
DK (1) | DK0620847T3 (de) |
ES (1) | ES2117672T3 (de) |
WO (1) | WO1993008266A1 (de) |
Families Citing this family (58)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US7049102B1 (en) | 1989-09-22 | 2006-05-23 | Board Of Trustees Of Leland Stanford University | Multi-gene expression profile |
US5851832A (en) * | 1991-07-08 | 1998-12-22 | Neurospheres, Ltd. | In vitro growth and proliferation of multipotent neural stem cells and their progeny |
US7361505B1 (en) | 1991-07-08 | 2008-04-22 | Neurospheres Holdings Ltd. | Multipotent neural stem cell compositions |
US6294346B1 (en) | 1991-07-08 | 2001-09-25 | Neurospheres Holdings, Ltd. | Use of multipotent neural stem cells and their progeny for the screening of drugs and other biological agents |
US6497872B1 (en) * | 1991-07-08 | 2002-12-24 | Neurospheres Holdings Ltd. | Neural transplantation using proliferated multipotent neural stem cells and their progeny |
US6399369B1 (en) * | 1991-07-08 | 2002-06-04 | Neurospheres Holdings Ltd. | Multipotent neural stem cell cDNA libraries |
US6071889A (en) * | 1991-07-08 | 2000-06-06 | Neurospheres Holdings Ltd. | In vivo genetic modification of growth factor-responsive neural precursor cells |
US7166277B1 (en) | 1991-07-08 | 2007-01-23 | Neurospheres Holdings Ltd. | Remyelination of neurons using multipotent neural stem cell progeny |
US5750376A (en) * | 1991-07-08 | 1998-05-12 | Neurospheres Holdings Ltd. | In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny |
US5980885A (en) * | 1991-07-08 | 1999-11-09 | Neurospheres Holdings Ltd. | Growth factor-induced proliferation of neural precursor cells in vivo |
US5792900A (en) * | 1991-10-21 | 1998-08-11 | The Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants |
US6214334B1 (en) * | 1991-10-21 | 2001-04-10 | Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants to treat neurodegenerative disorders and brain and spinal cord injuries |
US5175103A (en) * | 1991-10-21 | 1992-12-29 | Trustees Of University Of Pennsylvania | Preparation of pure cultures of post-mitotic human neurons |
CA2176216A1 (en) * | 1993-11-09 | 1995-05-18 | Virginia M.-Y. Lee | Compositions and methods for producing and using homogeneous neuronal cell transplants |
US7011827B2 (en) * | 1993-11-09 | 2006-03-14 | Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants |
US5449609A (en) * | 1994-01-31 | 1995-09-12 | Children's Hospital Of Philadelphia | Methods for screening for neurotoxicity using a clonal human teratocarcinoma cell line |
US20060029575A1 (en) * | 1994-11-09 | 2006-02-09 | Lee Virginia M | Compositions and methods for producing and using homogenous neuronal cell transplants |
EP0792350A1 (de) * | 1994-11-14 | 1997-09-03 | Neurospheres Holdings Ltd. | Proliferation regelung von neuralen stammzellen |
US5753505A (en) | 1995-07-06 | 1998-05-19 | Emory University | Neuronal progenitor cells and uses thereof |
US7544511B2 (en) * | 1996-09-25 | 2009-06-09 | Neuralstem Biopharmaceuticals Ltd. | Stable neural stem cell line methods |
US6162428A (en) * | 1997-02-12 | 2000-12-19 | Layton Bioscience, Inc. | hNT-neuron human neuronal cells to replace ganglion cells |
WO1998049271A1 (en) * | 1997-04-25 | 1998-11-05 | American Home Products Corporation | Human neuronal cell line |
US6254865B1 (en) | 1997-06-17 | 2001-07-03 | University Technology Corporation | Method of treating huntington's disease using HNT neurons |
US6235527B1 (en) * | 1997-11-29 | 2001-05-22 | University Of Utah Research Foundation | Lineage restricted glial precursors from the central nervous system |
US6734015B1 (en) * | 1997-07-04 | 2004-05-11 | University Of Utah Research Foundation | Isolation of lineage-restricted neuronal precursors |
EP1011732A4 (de) * | 1997-07-04 | 2002-10-23 | Univ Utah Res Found | Auf eine zelllinie beschränkter neuronaler vorläufer |
US5968829A (en) * | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
WO1999013726A1 (en) * | 1997-09-19 | 1999-03-25 | University Of South Florida | Neuronal cells and an immunosuppressant and anti-inflammatory factor |
WO2000006700A1 (en) * | 1998-07-29 | 2000-02-10 | Layton Bioscience, Inc. | Production and use of dopaminergic cells to treat dopaminergic deficiencies |
EP1949904A3 (de) | 1999-04-27 | 2008-08-06 | Layton Bioscience, Inc. | Zelltherapie für chronische Apoplexie |
US20030211085A1 (en) * | 2000-03-16 | 2003-11-13 | Sanberg Paul R. | Cell therapy for chronic stroke |
DE19928210B4 (de) * | 1999-06-19 | 2005-08-18 | Neuroprogen Gmbh Leipzig | Neuronales Zellmaterial und Verfahren zu dessen Herstellung |
US6749850B1 (en) | 1999-08-18 | 2004-06-15 | The General Hospital Corporation | Methods, compositions and kits for promoting recovery from damage to the central nervous system |
WO2001053503A1 (en) * | 2000-01-18 | 2001-07-26 | Cornell Research Foundation, Inc. | Neural progenitor cells from hippocampal tissue and a method for isolating and purifying them |
DE60123937T2 (de) | 2000-04-13 | 2007-06-14 | The Board of Regents, The University of Texas System, Austin | Behandlung von neuro-degenerativen gastrointestinalkrankheiten durch impantation von neuronalen stammzellen und/oder deren nachkommen in gastrointestinalorgane |
US7037493B2 (en) | 2000-05-01 | 2006-05-02 | Cornell Research Foundation, Inc. | Method of inducing neuronal production in the brain and spinal cord |
US6897061B1 (en) | 2000-06-16 | 2005-05-24 | Spinal Cord Society | Transdifferentiation of glial cells |
JP4031901B2 (ja) * | 2000-07-19 | 2008-01-09 | 株式会社東芝 | 固体撮像装置 |
WO2002028850A1 (fr) * | 2000-10-05 | 2002-04-11 | Takeda Chemical Industries, Ltd. | Promoteurs de proliferation et de differenciation de cellules embryonnaires et/ou de precurseurs neuronaux |
US20020192194A1 (en) * | 2000-12-05 | 2002-12-19 | Mcgrogan Michael | Production and use of dopaminergic cells to treat dopaminergic deficiencies |
WO2002099087A2 (de) * | 2001-06-05 | 2002-12-12 | Thomas Rohrmeier | Zellpopulationen zur auffindung neuronaler targets und potentieller wirkstoffe |
WO2003070189A2 (en) * | 2002-02-15 | 2003-08-28 | Cornell Research Foundation, Inc. | Enhancing neurotrophin-induced neurogenesis by endogenous neural progenitor cells by concurrent overexpression of brain derived neurotrophic factor and an inhibitor of a pro-gliogenic bone morphogenetic protein |
GB0217981D0 (en) * | 2002-08-02 | 2002-09-11 | Univ Durham | Method for preparation, purification and differentiation of neurospheres from mammalian stem cells |
US8293488B2 (en) | 2002-12-09 | 2012-10-23 | Neuralstem, Inc. | Method for screening neurogenic agents |
WO2004053071A2 (en) * | 2002-12-09 | 2004-06-24 | Judith Kelleher-Andersson | Method for discovering neurogenic agents |
DE10259617A1 (de) * | 2002-12-18 | 2004-07-08 | Cardion Ag | Verfahren zur Inhibierung des Proliferationspotentials von Zellpopulationen und Geweben |
US7485457B2 (en) * | 2003-04-17 | 2009-02-03 | United States Of America Department Of Veterans Affairs | Isolated/cloned human NT2 cell lines expressing serotonin and GABA |
FR2859219B1 (fr) * | 2003-09-02 | 2005-10-14 | Alain Privat | Procede de production de neurones a partir de cellules d'une lignee cellulaire |
EP3000877B1 (de) * | 2004-06-09 | 2019-08-14 | The University Court of the University of Edinburgh | Nervenstammzellen |
US7663614B2 (en) * | 2004-10-05 | 2010-02-16 | Jeff Maynard | Method for storing and comparing computer generated lines |
EP1814979B1 (de) | 2004-11-17 | 2015-04-08 | Neuralstem, Inc. | Transplantation menschlicher nervenzellen zur behandlung neurodegenerativer erkrankungen |
US7736641B2 (en) * | 2007-02-21 | 2010-06-15 | Prince Henry's Institute Of Medical Research | Role for SRY in Parkinson's disease |
US20090136461A1 (en) * | 2007-11-28 | 2009-05-28 | Moon Suk Kim | Neuronal differentiation method of adult stem cells using small molecules |
WO2010075500A1 (en) | 2008-12-23 | 2010-07-01 | Stemcells California, Inc | Target populations of oligodendrocyte precursor cells and methods of making and using same |
US9101570B1 (en) | 2009-02-13 | 2015-08-11 | Endocellutions, Inc. | Adult and neonatal stem cell therapy to treat diabetes through the repair of the gastrointestinal tract |
MX341316B (es) | 2010-07-28 | 2016-08-15 | Neuralstem Inc * | Poblacion de celulas madre neurales para tratar y/o revertir enfermedades y/o trastornos neurodegenerativos. |
JP6983069B2 (ja) | 2014-10-20 | 2021-12-17 | ニューラルステム, インコーポレイテッド | 成長因子をコードする外因性ポリヌクレオチドを含む安定な神経幹細胞およびその使用の方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4829000A (en) * | 1985-08-30 | 1989-05-09 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Reconstituted basement membrane complex with biological activity |
US5032407A (en) * | 1987-01-16 | 1991-07-16 | Ohio University Edison Animal Biotechnology Center | Gene transfer using transformed, neodetermined, embryonic cells |
US5270191A (en) * | 1988-04-12 | 1993-12-14 | Massachusetts Institute Of Technology | Method for manipulation of the cell types of eukaryotes |
FR2645866B1 (fr) * | 1989-04-17 | 1991-07-05 | Centre Nat Rech Scient | Nouvelles lipopolyamines, leur preparation et leur emploi |
US5180820A (en) * | 1989-08-30 | 1993-01-19 | Barde Yves Alain | Brain-derived neurotrophic factor |
US5175103A (en) * | 1991-10-21 | 1992-12-29 | Trustees Of University Of Pennsylvania | Preparation of pure cultures of post-mitotic human neurons |
-
1991
- 1991-10-21 US US07/780,715 patent/US5175103A/en not_active Expired - Lifetime
-
1992
- 1992-08-05 DE DE69225332T patent/DE69225332T2/de not_active Expired - Fee Related
- 1992-08-05 CA CA002120730A patent/CA2120730A1/en not_active Abandoned
- 1992-08-05 ES ES92917219T patent/ES2117672T3/es not_active Expired - Lifetime
- 1992-08-05 DK DK92917219T patent/DK0620847T3/da active
- 1992-08-05 AT AT92917219T patent/ATE165617T1/de not_active IP Right Cessation
- 1992-08-05 JP JP50765893A patent/JP3169611B2/ja not_active Expired - Fee Related
- 1992-08-05 WO PCT/US1992/006540 patent/WO1993008266A1/en active IP Right Grant
- 1992-08-05 EP EP92917219A patent/EP0620847B1/de not_active Expired - Lifetime
-
1996
- 1996-06-10 US US08/660,723 patent/US5654189A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE69225332T2 (de) | 1998-08-27 |
EP0620847A1 (de) | 1994-10-26 |
JPH07502645A (ja) | 1995-03-23 |
DK0620847T3 (da) | 1998-10-07 |
EP0620847B1 (de) | 1998-04-29 |
CA2120730A1 (en) | 1993-04-29 |
ATE165617T1 (de) | 1998-05-15 |
EP0620847A4 (de) | 1995-03-29 |
US5175103A (en) | 1992-12-29 |
JP3169611B2 (ja) | 2001-05-28 |
ES2117672T3 (es) | 1998-08-16 |
US5654189A (en) | 1997-08-05 |
WO1993008266A1 (en) | 1993-04-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8328 | Change in the person/name/address of the agent |
Representative=s name: VONNEMANN, KLOIBER & KOLLEGEN, 80796 MUENCHEN |
|
8339 | Ceased/non-payment of the annual fee |