DE69218586T2 - Verbessertes zweistoffgel aus galactomannan-agarose für die elektrophorese von nukleinsäure - Google Patents

Verbessertes zweistoffgel aus galactomannan-agarose für die elektrophorese von nukleinsäure

Info

Publication number
DE69218586T2
DE69218586T2 DE69218586T DE69218586T DE69218586T2 DE 69218586 T2 DE69218586 T2 DE 69218586T2 DE 69218586 T DE69218586 T DE 69218586T DE 69218586 T DE69218586 T DE 69218586T DE 69218586 T2 DE69218586 T2 DE 69218586T2
Authority
DE
Germany
Prior art keywords
agarose
galactomannan
gel
electrophoresis
improved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69218586T
Other languages
English (en)
Other versions
DE69218586D1 (de
Inventor
Daniel Perlman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Publication of DE69218586D1 publication Critical patent/DE69218586D1/de
Application granted granted Critical
Publication of DE69218586T2 publication Critical patent/DE69218586T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/12—Agar or agar-agar, i.e. mixture of agarose and agaropectin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/14—Hemicellulose; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S522/00—Synthetic resins or natural rubbers -- part of the class 520 series
    • Y10S522/911—Specified treatment involving megarad or less
DE69218586T 1991-01-24 1992-01-24 Verbessertes zweistoffgel aus galactomannan-agarose für die elektrophorese von nukleinsäure Expired - Fee Related DE69218586T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/645,203 US5230832A (en) 1991-01-24 1991-01-24 Galactomannan-agarose binary gel for nucleic acid electrophoresis
PCT/US1992/000598 WO1992012789A1 (en) 1991-01-24 1992-01-24 Improved galactomannan-agarose binary gel for nucleic acid electrophoresis

Publications (2)

Publication Number Publication Date
DE69218586D1 DE69218586D1 (de) 1997-04-30
DE69218586T2 true DE69218586T2 (de) 1997-07-03

Family

ID=24588054

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69218586T Expired - Fee Related DE69218586T2 (de) 1991-01-24 1992-01-24 Verbessertes zweistoffgel aus galactomannan-agarose für die elektrophorese von nukleinsäure

Country Status (8)

Country Link
US (1) US5230832A (de)
EP (1) EP0569494B1 (de)
JP (1) JPH0786503B2 (de)
AT (1) ATE150664T1 (de)
DE (1) DE69218586T2 (de)
DK (1) DK0569494T3 (de)
ES (1) ES2099249T3 (de)
WO (1) WO1992012789A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5885432A (en) * 1992-11-05 1999-03-23 Soane Biosciences Un-crosslinked polymeric media for electrophoresis
DE19920688A1 (de) * 1999-05-05 2000-11-23 Manfred Kuhn Radiolytisch modifizierte Heteropolysaccharide
US6586590B1 (en) * 2000-07-03 2003-07-01 Marine Bioproducts International Clarified hydrocolloids of undiminished properties and method of producing same
US7408057B2 (en) * 2000-07-03 2008-08-05 Marine Bioproducts Intenational Clarified hydrocolloids of undiminished properties and method of producing same
GB0316742D0 (en) * 2003-07-17 2003-08-20 Fermentas Uab Electrophoretic gels and their manufacture
JP7072830B2 (ja) * 2017-07-26 2022-05-23 国立大学法人広島大学 高分離能を有する電気泳動用ゲルの製造方法およびその製造キット
JP6966071B2 (ja) * 2017-11-29 2021-11-10 国立大学法人広島大学 ゲル分離能向上剤の製造方法およびゲル分離能向上剤の利用
US11691122B2 (en) 2018-11-02 2023-07-04 Advanced Aesthetic Technologies, Inc. Irradiated agarose, compositions thereof, and related methods
CN112996551B (zh) * 2018-11-02 2023-08-29 先进美学科技公司 辐照琼脂糖、其组合物及相关方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3034974A (en) * 1958-01-28 1962-05-15 Gen Foods Corp Polysaccharide extraction by irradiation
FR2357188A2 (fr) * 1976-07-09 1978-02-03 Ceca Sa Perfectionnements aux compositions gelifiantes pour produits alimentaires
US4290911A (en) * 1979-02-07 1981-09-22 Fmc Corporation Agarose composition, aqueous gel and method of making same
US4321121A (en) * 1981-01-19 1982-03-23 Beckman Instruments, Inc. Electrophoretic technique for separating lactate dehydrogenase isoenzymes and improved electrophoretic gel for use therein
US4704198A (en) * 1984-04-27 1987-11-03 E. I. Du Pont De Nemours And Company Porosity gradient electrophoresis gel
IT1213384B (it) * 1986-11-24 1989-12-20 Lab Derivati Organici Mediolan Processo per la preparazione controllata di gilcosaminoglicani a basso peso molecolare.
JPH0786501B2 (ja) * 1989-01-13 1995-09-20 エフ エム シー コーポレーション 多糖類分割ゲル及び積層電気泳動用ゲルシステム
US4983268A (en) * 1989-08-03 1991-01-08 Fmc Corporation High gel strength low electroendosmosis agarose
EP0455841A1 (de) * 1990-05-05 1991-11-13 Studiengesellschaft Kohle mbH Verfahren zur Herstellung von mit Gel gefüllten Kapillaren für kapillare Gelelektrophorese

Also Published As

Publication number Publication date
DE69218586D1 (de) 1997-04-30
JPH06503174A (ja) 1994-04-07
JPH0786503B2 (ja) 1995-09-20
EP0569494A1 (de) 1993-11-18
EP0569494B1 (de) 1997-03-26
ATE150664T1 (de) 1997-04-15
US5230832A (en) 1993-07-27
ES2099249T3 (es) 1997-05-16
WO1992012789A1 (en) 1992-08-06
DK0569494T3 (da) 1997-04-14
EP0569494A4 (de) 1994-01-19

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee