DE69133525T8 - Verfahren zur Herstellung von recombinantem Hepatitis B Oberflächenantigen mit erhöhter Immunogen-Kapazität und Verwendung in einer Impfstoffzubereitung - Google Patents

Verfahren zur Herstellung von recombinantem Hepatitis B Oberflächenantigen mit erhöhter Immunogen-Kapazität und Verwendung in einer Impfstoffzubereitung Download PDF

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Publication number
DE69133525T8
DE69133525T8 DE69133525T DE69133525T DE69133525T8 DE 69133525 T8 DE69133525 T8 DE 69133525T8 DE 69133525 T DE69133525 T DE 69133525T DE 69133525 T DE69133525 T DE 69133525T DE 69133525 T8 DE69133525 T8 DE 69133525T8
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DE
Germany
Prior art keywords
surface antigen
immunogen
capacity
increased
vaccine preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69133525T
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English (en)
Other versions
DE69133525T2 (de
DE69133525D1 (de
Inventor
González Verena L. Plaza de la Revolucion Muzio
Eduardo Penton Arias
Giuvel Eden Fontirrochi Escobar
Marcelo Puentes Grandes Nazábal Gálvez
Marta de Jesus Lawton 10 de Octubre González Griego
Alejandro Marianao Beldarrain Iznaga
Guillermo Julio Cubanacan Playa Padron Gonzalez
Victoria Ramirez Albajes
Arnaldo Garcia Pena
Carlos Enrique Ruiz Cruz
Gladys Mabel Vibora 10 de Oct. Izquierdo Lopez
Luis Saturnino Herrera Martinez
Carlos Antonio Center Habana Duarte Cano
Lilia Luisa Playa Perez Suarez
Jose Garcia Suárez
Gustavo Alberto Vibora de la Riva de la Riva
Ramon Alexis Lisa Santiago Avila
Filiberto Rosendo Playa Ayon Zorrilla
Rolando Paez Meireles
Alberto Playa Agraz Fierro
Raul Enrique Playa Diaz Betancourt
Yair Playa Quinones Maya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centro de Ingenieria Genetica y Biotecnologia CIGB
Original Assignee
Centro de Ingenieria Genetica y Biotecnologia CIGB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro de Ingenieria Genetica y Biotecnologia CIGB filed Critical Centro de Ingenieria Genetica y Biotecnologia CIGB
Application granted granted Critical
Publication of DE69133525D1 publication Critical patent/DE69133525D1/de
Publication of DE69133525T2 publication Critical patent/DE69133525T2/de
Publication of DE69133525T8 publication Critical patent/DE69133525T8/de
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C07K16/082—Hepadnaviridae, e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Communicable Diseases (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE69133525T 1990-10-08 1991-10-07 Verfahren zur Herstellung von recombinantem Hepatitis B Oberflächenantigen mit erhöhter Immunogen-Kapazität und Verwendung in einer Impfstoffzubereitung Expired - Fee Related DE69133525T8 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU90155A CU22290A1 (es) 1990-10-08 1990-10-08 Procedimiento para la obtencion de antigeno de superficie del virus de la hepatitis b de superior capacidad inmunoganica y su uso en un preparado vacunal
CU15590 1990-10-08

Publications (3)

Publication Number Publication Date
DE69133525D1 DE69133525D1 (de) 2006-06-01
DE69133525T2 DE69133525T2 (de) 2006-11-23
DE69133525T8 true DE69133525T8 (de) 2007-03-08

Family

ID=5459466

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69133525T Expired - Fee Related DE69133525T8 (de) 1990-10-08 1991-10-07 Verfahren zur Herstellung von recombinantem Hepatitis B Oberflächenantigen mit erhöhter Immunogen-Kapazität und Verwendung in einer Impfstoffzubereitung
DE69130724T Expired - Fee Related DE69130724T2 (de) 1990-10-08 1991-10-07 Verfahren zur Gewinnung von rekombinantem Hepatitis B Virus (HEP B) Oberflächeantigen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69130724T Expired - Fee Related DE69130724T2 (de) 1990-10-08 1991-10-07 Verfahren zur Gewinnung von rekombinantem Hepatitis B Virus (HEP B) Oberflächeantigen

Country Status (9)

Country Link
EP (2) EP0480525B1 (de)
JP (2) JP3189212B2 (de)
AT (2) ATE175445T1 (de)
AU (1) AU649482B2 (de)
CU (1) CU22290A1 (de)
DE (2) DE69133525T8 (de)
DK (2) DK0480525T3 (de)
ES (2) ES2264183T3 (de)
GR (1) GR3029582T3 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU95109861A (ru) * 1994-06-09 1997-06-10 Сентро Де Инженьериа Генетика И Биотекнологиа (Cu) Рекомбинантный фрагмент антитела, применение scfv, применение фрагмента антитела или пептида, способ получения scfv
KR960023066A (ko) * 1994-12-10 1996-07-18 성재갑 전에스 (s) 2 펩티드 함유 비 (b) 형 간염 표면항원의 정제 방법
CU22642A1 (es) * 1996-12-12 2000-12-22 Ct Ingenieria Genetica Biotech Secuencia de adnc derivadas del genoma del virus de la hepatitis c y su uso
US6436402B1 (en) 1999-10-15 2002-08-20 Merck & Co., Inc. Process for making human papillomavirus virus-like particles with improved properties
EP1403274A1 (de) * 2002-09-30 2004-03-31 Meristem Therapeutics Verfahren zur Reinigung von rekombinanten Proteinen aus komplexen Medien sowie die auf diese Weise erhaltenen gereinigten Proteine
CU23405A1 (es) * 2003-10-20 2009-08-04 Ct Ingenieria Genetica Biotech Composiciones farmacéuticas para uso terapéutico
JP4936272B2 (ja) * 2006-02-13 2012-05-23 独立行政法人科学技術振興機構 バイオナノカプセルの効率的な精製法
CU23740A1 (es) 2009-09-29 2011-12-28 Ct Ingenieria Genetica Biotech Método de obtención de una formulación de antígenos del virus de la hepatitis b
JP6981992B2 (ja) 2016-03-31 2021-12-17 セントロ デ インジエニエリア ジエネテイカ イ バイオテクノロジア Ｂ型肝炎ウイルスの表面及び核内抗原を含む医薬組成物
KR102630293B1 (ko) * 2021-06-21 2024-01-29 선지호 박스 포장 테이프 절단용 칼날유닛과 이를 이용한 박스 포장 테이프 절단장치.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59101426A (ja) * 1982-11-29 1984-06-12 Green Cross Corp:The Ｂ型肝炎感染予防用ワクチンの製造方法
US4624918A (en) * 1984-07-09 1986-11-25 Genentech, Inc. Purification process for hepatitis surface antigen and product thereof
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
DE3882154T2 (de) * 1987-02-27 1993-12-02 Merck & Co Inc Verfahren zur Herstellung des pres 1/S2/S-Hepatitis-B-Antigens aus Hefe.
US5004688A (en) * 1988-04-15 1991-04-02 Phillips Petroleum Company Purification of hepatitis proteins
NZ228774A (en) * 1988-04-25 1991-05-28 Phillips Petroleum Co Hbv particles containing pres 2 proteins and recombinant processes for their production in yeast cells
KR900008591B1 (ko) * 1988-05-13 1990-11-26 동아제약 주식회사 항이디오형 항체를 용출제로 사용하는 면역친화성 크로마토그라프법에 의한 생리활성물질의 정제방법

Also Published As

Publication number Publication date
JPH0576370A (ja) 1993-03-30
GR3029582T3 (en) 1999-06-30
EP0864649B1 (de) 2006-04-26
DE69133525T2 (de) 2006-11-23
EP0864649A2 (de) 1998-09-16
CU22290A1 (es) 1995-01-31
ATE175445T1 (de) 1999-01-15
DK0864649T3 (da) 2006-08-28
DE69133525D1 (de) 2006-06-01
AU8557491A (en) 1992-04-30
DE69130724D1 (de) 1999-02-18
EP0480525A2 (de) 1992-04-15
JP3242640B2 (ja) 2001-12-25
ATE324451T1 (de) 2006-05-15
DE69130724T2 (de) 1999-06-17
EP0480525A3 (en) 1992-09-30
ES2129025T3 (es) 1999-06-01
DK0480525T3 (da) 1999-08-30
EP0480525B1 (de) 1999-01-07
ES2264183T3 (es) 2006-12-16
EP0864649A3 (de) 1998-11-11
JP3189212B2 (ja) 2001-07-16
AU649482B2 (en) 1994-05-26
JP2001231582A (ja) 2001-08-28

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Legal Events

Date Code Title Description
8381 Inventor (new situation)

Inventor name: MUZIO, GONZALEZ, VERENA L., PLAZA DE LA REVOLUCION

Inventor name: PENTON ARIAS, EDUARDO, CIUDAD DE LA HABANA, CU

Inventor name: FONTIRROCHI ESCOBAR, GIUVEL, EDEN, CAMAGüEY, CU

Inventor name: NAZABAL GALVEZ, MARCELO, PUENTES GRANDES, PLAYA. C

Inventor name: GONZALEZ GRIEGO, MARTA DE JESUS, LAWTON, 10 DE OCT

Inventor name: BELDARRAIN IZNAGA, ALEJANDRO, MARIANAO, CIUDAD DE

Inventor name: SANTIAGO AVILA, RAMON ALEXIS, LISA, CIUDAD DE LA H

Inventor name: DE LA RIVA DE LA RIVA, GUSTAVO ALBERTO, VIBORA, CI

Inventor name: GARCIA SUAREZ, JOSE, PLAZA C.DE LA HABANA, CU

Inventor name: PEREZ SUAREZ, LILIA LUISA, PLAYA, CIUDAD DE LA HAB

Inventor name: DUARTE CANO, CARLOS ANTONIO, CENTER HABANA, CIUDAD

Inventor name: HERRERA MARTINEZ, LUIS SATURNINO, CIUDAD DE LA HAB

Inventor name: IZQUIERDO LOPEZ, GLADYS MABEL, VIBORA, 10 DE OCT.,

Inventor name: RUIZ CRUZ, CARLOS ENRIQUE, CIUDAD DE LA HABANA, CU

Inventor name: GARCIA PENA, ARNALDO, CIUDAD DE LA HABANA, CU

Inventor name: RAMIREZ ALBAJES, VICTORIA, PLAYA CIUDAD DE LA HABA

Inventor name: PADRON GONZALEZ, GUILLERMO JULIO, CUBANACAN PLAYA,

Inventor name: QUINONES MAYA, YAIR, PLAYA, CIUDAD DE LA HABANA, C

Inventor name: DIAZ BETANCOURT, RAUL ENRIQUE, PLAYA, CIUDAD DE LA

Inventor name: AGRAZ FIERRO, ALBERTO, PLAYA, CIUDAD DE LA HABANA,

Inventor name: PAEZ MEIRELES, ROLANDO, LA HABANA, CU

Inventor name: AYON ZORRILLA, FILIBERTO ROSENDO, PLAYA, CIUDAD DE

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee