DE69126248D1 - Immunchromatographischer assay und verfahren zur nutzung desselben - Google Patents
Immunchromatographischer assay und verfahren zur nutzung desselbenInfo
- Publication number
- DE69126248D1 DE69126248D1 DE69126248T DE69126248T DE69126248D1 DE 69126248 D1 DE69126248 D1 DE 69126248D1 DE 69126248 T DE69126248 T DE 69126248T DE 69126248 T DE69126248 T DE 69126248T DE 69126248 D1 DE69126248 D1 DE 69126248D1
- Authority
- DE
- Germany
- Prior art keywords
- assays
- immunchromatographic
- assay
- preparing
- lat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/814—Pregnancy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US476788 | 1990-02-08 | ||
US07/476,788 US5096837A (en) | 1990-02-08 | 1990-02-08 | Immunochromatographic assay and method of using same |
PCT/US1991/000896 WO1991012336A1 (en) | 1990-02-08 | 1991-02-08 | Immunochromatographic assay and method of using same |
Publications (3)
Publication Number | Publication Date |
---|---|
DE69126248D1 true DE69126248D1 (de) | 1997-07-03 |
DE69126248T2 DE69126248T2 (de) | 1997-09-25 |
DE69126248T3 DE69126248T3 (de) | 2008-01-24 |
Family
ID=23893251
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69126248T Expired - Fee Related DE69126248T3 (de) | 1990-02-08 | 1991-02-08 | Immunchromatographischer assay und verfahren zur nutzung desselben |
Country Status (10)
Country | Link |
---|---|
US (1) | US5096837A (de) |
EP (1) | EP0466914B2 (de) |
JP (1) | JP3317699B2 (de) |
KR (1) | KR920701471A (de) |
AT (1) | ATE153701T1 (de) |
AU (1) | AU649022B2 (de) |
CA (1) | CA2049919C (de) |
DE (1) | DE69126248T3 (de) |
ES (1) | ES2103803T5 (de) |
WO (1) | WO1991012336A1 (de) |
Families Citing this family (76)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6168956B1 (en) | 1991-05-29 | 2001-01-02 | Beckman Coulter, Inc. | Multiple component chromatographic assay device |
US5998220A (en) | 1991-05-29 | 1999-12-07 | Beckman Coulter, Inc. | Opposable-element assay devices, kits, and methods employing them |
US5877028A (en) | 1991-05-29 | 1999-03-02 | Smithkline Diagnostics, Inc. | Immunochromatographic assay device |
AU670108B2 (en) | 1992-09-11 | 1996-07-04 | Becton Dickinson & Company | Improved antibodies to plasmodium falciparum |
US5837546A (en) * | 1993-08-24 | 1998-11-17 | Metrika, Inc. | Electronic assay device and method |
DE4434093A1 (de) * | 1994-09-23 | 1996-03-28 | Boehringer Mannheim Gmbh | Verfahren zum qualitativen oder/und quantitativen Nachweis einer zu bestimmenden Substanz |
US5753517A (en) * | 1996-03-29 | 1998-05-19 | University Of British Columbia | Quantitative immunochromatographic assays |
US5968839A (en) * | 1996-05-13 | 1999-10-19 | Metrika, Inc. | Method and device producing a predetermined distribution of detectable change in assays |
US6165798A (en) * | 1996-10-10 | 2000-12-26 | University Of British Columbia | Optical quantification of analytes in membranes |
CA2269097C (en) | 1996-11-08 | 2007-01-09 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Synthesis and purification of hepatitis c virus-like particles |
US5879951A (en) | 1997-01-29 | 1999-03-09 | Smithkline Diagnostics, Inc. | Opposable-element assay device employing unidirectional flow |
US20050101032A1 (en) * | 1997-02-10 | 2005-05-12 | Metrika, Inc. | Assay device, composition, and method of optimizing assay sensitivity |
US5939252A (en) | 1997-05-09 | 1999-08-17 | Lennon; Donald J. | Detachable-element assay device |
US6194222B1 (en) | 1998-01-05 | 2001-02-27 | Biosite Diagnostics, Inc. | Methods for monitoring the status of assays and immunoassays |
US20060019404A1 (en) * | 1998-05-06 | 2006-01-26 | Blatt Joel M | Quantitative assay with extended dynamic range |
US6136610A (en) | 1998-11-23 | 2000-10-24 | Praxsys Biosystems, Inc. | Method and apparatus for performing a lateral flow assay |
US6790661B1 (en) * | 1999-07-16 | 2004-09-14 | Verax Biomedical, Inc. | System for detecting bacteria in blood, blood products, and fluids of tissues |
US6316205B1 (en) | 2000-01-28 | 2001-11-13 | Genelabs Diagnostics Pte Ltd. | Assay devices and methods of analyte detection |
US6699722B2 (en) | 2000-04-14 | 2004-03-02 | A-Fem Medical Corporation | Positive detection lateral-flow apparatus and method for small and large analytes |
US6767710B2 (en) * | 2001-03-30 | 2004-07-27 | Praxsys Biosystems, Llc | Prewetting stop flow test strip |
EP1417491A4 (de) * | 2001-07-18 | 2004-08-11 | Siliang Zhou | Teststreifen für einen lateralfluss-assay für eine ganze zellen enthaltende probe |
US7456025B2 (en) * | 2001-08-28 | 2008-11-25 | Porex Corporation | Sintered polymer membrane for analyte detection device |
US8481334B1 (en) | 2001-11-06 | 2013-07-09 | Charm Sciences, Inc. | Method of attaching a ligand to a solid support |
US20030175991A1 (en) * | 2002-03-18 | 2003-09-18 | Ho Winston Z. | Optically-assisted high precision pregnancy progress monitoring |
US7560272B2 (en) | 2003-01-04 | 2009-07-14 | Inverness Medical Switzerland Gmbh | Specimen collection and assay container |
US7517495B2 (en) * | 2003-08-25 | 2009-04-14 | Inverness Medical Switzerland Gmbh | Biological specimen collection and analysis system |
JP4933258B2 (ja) | 2003-09-22 | 2012-05-16 | クイデル コーポレーション | 試料中の複数分析物の検出装置 |
US7150995B2 (en) * | 2004-01-16 | 2006-12-19 | Metrika, Inc. | Methods and systems for point of care bodily fluid analysis |
US20050227370A1 (en) * | 2004-03-08 | 2005-10-13 | Ramel Urs A | Body fluid analyte meter & cartridge system for performing combined general chemical and specific binding assays |
US7632687B2 (en) * | 2004-03-23 | 2009-12-15 | Quidel Corporation | Hybrid phase lateral flow assay |
ATE479096T1 (de) * | 2004-07-29 | 2010-09-15 | Relia Diagnostic Systems Llc | Querstromsystem und assay |
US8475735B2 (en) * | 2004-11-01 | 2013-07-02 | Uma Mahesh Babu | Disposable immunodiagnostic test system |
EP1872136B9 (de) | 2005-04-04 | 2023-02-08 | Biogen MA Inc. | Verfahren und stoffe zur bewertung einer immunantwort auf ein therapeutisches protein |
WO2008002462A2 (en) | 2006-06-23 | 2008-01-03 | Micronics, Inc. | Methods and devices for microfluidic point-of-care immunoassays |
US9056291B2 (en) | 2005-11-30 | 2015-06-16 | Micronics, Inc. | Microfluidic reactor system |
US7763453B2 (en) | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
US7871568B2 (en) * | 2006-01-23 | 2011-01-18 | Quidel Corporation | Rapid test apparatus |
US7794656B2 (en) * | 2006-01-23 | 2010-09-14 | Quidel Corporation | Device for handling and analysis of a biological sample |
LT2676967T (lt) | 2006-02-28 | 2019-09-10 | Biogen Ma Inc. | Uždegiminių ir autoimuninių ligų gydymo būdai su natalizumabu |
BRPI0708392A2 (pt) | 2006-03-03 | 2011-05-24 | Elan Pharm Inc | métodos de tratamento de um paciente com uma doença inflamatória ou autoimune com natalizumab e de uso de natalizumab para tratar um paciente com uma doença inflamatória ou autoimune |
US7527765B2 (en) * | 2006-04-11 | 2009-05-05 | Harrogate Holdings | Consumer food testing device |
US20110184747A1 (en) * | 2006-08-09 | 2011-07-28 | Carmen Bozic | Method for distribution of a drug |
WO2008061130A2 (en) * | 2006-11-15 | 2008-05-22 | Ucp Biosciences, Inc. | An improved collecting and testing device and method of use |
US9086408B2 (en) | 2007-04-30 | 2015-07-21 | Nexus Dx, Inc. | Multianalyte assay |
ES2609818T3 (es) | 2007-06-15 | 2017-04-24 | Xiamen University | Anticuerpos monoclonales que se unen a hemaglutinina del virus de la gripe aviar del subtipo H5 y usos de los mismos |
US8692873B2 (en) | 2009-01-15 | 2014-04-08 | Alverix, Inc. | Video-frame data receiver with low frame capture rate |
US8422740B2 (en) | 2009-01-15 | 2013-04-16 | Scott Dylewski | Methods for determining a liquid front position on a test strip |
US20120015350A1 (en) * | 2009-03-10 | 2012-01-19 | Northwestern University | Lateral flow strip and uses thereof |
US8377643B2 (en) | 2009-03-16 | 2013-02-19 | Abaxis, Inc. | Split flow device for analyses of specific-binding partners |
NZ596333A (en) * | 2009-04-15 | 2014-05-30 | Relia Diagnostic Systems Inc | Expanding the dynamic range of a test strip |
ES2461992T3 (es) | 2009-10-09 | 2014-05-22 | Invisible Sentinel, Inc. | Dispositivo para la detección de antígenos y usos de los mismos |
ES2666372T3 (es) | 2009-10-11 | 2018-05-04 | Biogen Ma Inc. | Ensayos relacionados con anti-VLA-4 |
KR20120107497A (ko) | 2009-12-17 | 2012-10-02 | 아박시스, 인크. | 샘플 내 분석물을 검출하기 위한 신규 분석 및 그것과 관련된 키트 및 조성물 |
US11287423B2 (en) | 2010-01-11 | 2022-03-29 | Biogen Ma Inc. | Assay for JC virus antibodies |
PL2524060T3 (pl) | 2010-01-11 | 2018-05-30 | Biogen Ma Inc. | Test wykrywający przeciwciała przeciwko wirusowi jc |
KR101851117B1 (ko) | 2010-01-29 | 2018-04-23 | 마이크로닉스 인코포레이티드. | 샘플-투-앤서 마이크로유체 카트리지 |
US10295472B2 (en) | 2010-05-05 | 2019-05-21 | Alverix, Inc. | Assay reader operable to scan a test strip |
KR20130032895A (ko) | 2010-06-17 | 2013-04-02 | 아박시스, 인크. | 면역분석용 로터 |
EP2668501B1 (de) | 2011-01-27 | 2019-06-12 | Invisible Sentinel, Inc. | Analytnachweisvorrichtungen, multiplex- und tischplattenvorrichtungen für den nachweis von analyten und verwendungen davon |
KR102292563B1 (ko) | 2011-05-31 | 2021-08-24 | 바이오젠 엠에이 인코포레이티드 | Pml의 위험을 판단하는 방법 |
US8211715B1 (en) | 2011-11-15 | 2012-07-03 | Harrogate Holdings, Ltd. Co. | Consumer food testing device providing remote monitoring |
US9140693B2 (en) | 2011-12-23 | 2015-09-22 | Abbott Point Of Care Inc. | Integrated test device for optical detection of microarrays |
US9377475B2 (en) | 2011-12-23 | 2016-06-28 | Abbott Point Of Care Inc. | Optical assay device with pneumatic sample actuation |
WO2013096801A1 (en) | 2011-12-23 | 2013-06-27 | Abbott Point Of Care Inc | Reader devices for optical and electrochemical test devices |
WO2013134503A2 (en) | 2012-03-09 | 2013-09-12 | Invisible Sentinel, Inc. | Methods And Compositions For Detecting Multiple Analytes With A Single Signal |
CN104919035B (zh) | 2012-12-21 | 2017-08-11 | 精密公司 | 便携式荧光检测系统和微测定盒 |
EP2935908B1 (de) | 2012-12-21 | 2019-08-14 | PerkinElmer Health Sciences, Inc. | Fluidkreisläufe und zugehörige herstellungsverfahren |
JP6935167B2 (ja) | 2012-12-21 | 2021-09-15 | ペルキネルマー ヘルス サイエンシーズ, インコーポレイテッド | マイクロ流体使用のための低弾性フィルム |
JP2015072249A (ja) * | 2013-03-29 | 2015-04-16 | 富士フイルム株式会社 | 被検物質の測定方法、被検物質測定キット及び被検物質測定試薬 |
US10190153B2 (en) | 2013-05-07 | 2019-01-29 | Micronics, Inc. | Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions |
EP2994543B1 (de) | 2013-05-07 | 2018-08-15 | Micronics, Inc. | Vorrichtung zur herstellung und analyse von nukleinsäuren |
WO2014182844A1 (en) | 2013-05-07 | 2014-11-13 | Micronics, Inc. | Microfluidic devices and methods for performing serum separation and blood cross-matching |
EP3004334A4 (de) | 2013-05-28 | 2016-12-21 | Biogen Ma Inc | Verfahren zur beurteilung eines pml-risikos |
JP6118761B2 (ja) * | 2014-06-05 | 2017-04-19 | 富士フイルム株式会社 | 被検物質測定キット及び被検物質の測定方法 |
MX2018001353A (es) | 2015-08-06 | 2018-05-22 | Lia Diagnostics Inc | Ensayos dispersables en agua. |
EP3980182A1 (de) | 2019-06-04 | 2022-04-13 | Abbott Toxicology Limited | Analyse von flüssigkeitsproben |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4169138A (en) * | 1974-05-29 | 1979-09-25 | Pharmacia Diagnostics Ab | Method for the detection of antibodies |
CA1101330A (en) * | 1977-09-19 | 1981-05-19 | Ernst A. Fischer | Immunological material bonded to carboxylated latex polymer and process for making it |
GB2051360B (en) * | 1979-01-18 | 1983-05-18 | Unilever Ltd | Processes and materials for detecting and determining proteinaceous specific binding agents and materials bindable thereto |
NL8000173A (nl) * | 1980-01-11 | 1981-08-03 | Akzo Nv | Toepassing van in water dispergeerbare, hydrofobe kleurstoffen als label in immunochemische testen. |
US4376110A (en) * | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
JPS5748658A (en) * | 1980-09-08 | 1982-03-20 | Teikoku Hormone Mfg Co Ltd | Immunologic measuring method and reagent for measurement |
US4351824A (en) * | 1981-02-05 | 1982-09-28 | Icl Scientific | Polystyrene latex reagents, methods of preparation, and use in immunological procedures |
US4419453A (en) * | 1981-09-28 | 1983-12-06 | The Dow Chemical Company | Immunological agglutination assays with dyed or colored latex and kits |
US4419435A (en) * | 1982-05-21 | 1983-12-06 | Eastman Kodak Company | Photographic products and processes employing 6-heterocyclylazo-3-pyridinol nondiffusible cyan dye-releasing compounds and precursors thereof |
US4591570A (en) * | 1983-02-02 | 1986-05-27 | Centocor, Inc. | Matrix of antibody-coated spots for determination of antigens |
US4496654A (en) * | 1983-04-08 | 1985-01-29 | Quidel | Detection of HCG with solid phase support having avidin coating |
US4552839A (en) * | 1983-08-01 | 1985-11-12 | Syntex (U.S.A.) Inc. | Determination of analytes in particle-containing medium |
US4703017C1 (en) * | 1984-02-14 | 2001-12-04 | Becton Dickinson Co | Solid phase assay with visual readout |
US4632901A (en) * | 1984-05-11 | 1986-12-30 | Hybritech Incorporated | Method and apparatus for immunoassays |
JPS60256057A (ja) * | 1984-06-01 | 1985-12-17 | Dai Ichi Pure Chem Co Ltd | 免疫学的測定法 |
ATE195022T1 (de) * | 1987-04-27 | 2000-08-15 | Unilever Nv | Spezifische bindungstestverfahren |
US4855240A (en) * | 1987-05-13 | 1989-08-08 | Becton Dickinson And Company | Solid phase assay employing capillary flow |
US4912034A (en) * | 1987-09-21 | 1990-03-27 | Biogenex Laboratories | Immunoassay test device and method |
CA1312544C (en) * | 1987-12-21 | 1993-01-12 | James L. Wilcox | Chromatographic binding assay devices and methods |
-
1990
- 1990-02-08 US US07/476,788 patent/US5096837A/en not_active Expired - Lifetime
-
1991
- 1991-02-08 AT AT91904867T patent/ATE153701T1/de not_active IP Right Cessation
- 1991-02-08 EP EP91904867A patent/EP0466914B2/de not_active Expired - Lifetime
- 1991-02-08 DE DE69126248T patent/DE69126248T3/de not_active Expired - Fee Related
- 1991-02-08 JP JP50470691A patent/JP3317699B2/ja not_active Expired - Fee Related
- 1991-02-08 AU AU73162/91A patent/AU649022B2/en not_active Ceased
- 1991-02-08 ES ES91904867T patent/ES2103803T5/es not_active Expired - Lifetime
- 1991-02-08 WO PCT/US1991/000896 patent/WO1991012336A1/en active IP Right Grant
- 1991-02-08 KR KR1019910701267A patent/KR920701471A/ko not_active Application Discontinuation
- 1991-02-08 CA CA002049919A patent/CA2049919C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ATE153701T1 (de) | 1997-06-15 |
WO1991012336A1 (en) | 1991-08-22 |
DE69126248T2 (de) | 1997-09-25 |
ES2103803T5 (es) | 2007-12-16 |
EP0466914B1 (de) | 1997-05-28 |
CA2049919A1 (en) | 1991-08-09 |
JPH04504764A (ja) | 1992-08-20 |
JP3317699B2 (ja) | 2002-08-26 |
CA2049919C (en) | 1996-11-19 |
EP0466914A1 (de) | 1992-01-22 |
AU7316291A (en) | 1991-09-03 |
AU649022B2 (en) | 1994-05-12 |
EP0466914A4 (en) | 1992-06-24 |
ES2103803T3 (es) | 1997-10-01 |
KR920701471A (ko) | 1992-08-11 |
EP0466914B2 (de) | 2007-06-06 |
DE69126248T3 (de) | 2008-01-24 |
US5096837A (en) | 1992-03-17 |
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