DE69121975T2 - Spezifischer Nachweis von Mycobacterium Tuberculosis - Google Patents
Spezifischer Nachweis von Mycobacterium TuberculosisInfo
- Publication number
- DE69121975T2 DE69121975T2 DE69121975T DE69121975T DE69121975T2 DE 69121975 T2 DE69121975 T2 DE 69121975T2 DE 69121975 T DE69121975 T DE 69121975T DE 69121975 T DE69121975 T DE 69121975T DE 69121975 T2 DE69121975 T2 DE 69121975T2
- Authority
- DE
- Germany
- Prior art keywords
- sequence
- sequences
- dna
- bases
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000187479 Mycobacterium tuberculosis Species 0.000 title claims abstract description 21
- 238000011895 specific detection Methods 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 12
- 230000000295 complement effect Effects 0.000 claims abstract description 7
- 108020004414 DNA Proteins 0.000 claims description 36
- 239000012634 fragment Substances 0.000 claims description 35
- 230000003321 amplification Effects 0.000 claims description 33
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 33
- 239000000523 sample Substances 0.000 claims description 29
- 239000012472 biological sample Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 20
- 238000009396 hybridization Methods 0.000 claims description 15
- 238000001514 detection method Methods 0.000 claims description 11
- 239000013612 plasmid Substances 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 238000012795 verification Methods 0.000 claims description 8
- 238000004458 analytical method Methods 0.000 claims description 6
- 241000186359 Mycobacterium Species 0.000 claims description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 238000001502 gel electrophoresis Methods 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 239000013615 primer Substances 0.000 description 87
- 201000008827 tuberculosis Diseases 0.000 description 23
- 238000003752 polymerase chain reaction Methods 0.000 description 8
- 239000011543 agarose gel Substances 0.000 description 7
- 241000894007 species Species 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000186366 Mycobacterium bovis Species 0.000 description 5
- 241000187919 Mycobacterium microti Species 0.000 description 4
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
- 208000030507 AIDS Diseases 0.000 description 3
- 241000186367 Mycobacterium avium Species 0.000 description 3
- 241001467552 Mycobacterium bovis BCG Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 229960005542 ethidium bromide Drugs 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 208000027531 mycobacterial infectious disease Diseases 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 206010062207 Mycobacterial infection Diseases 0.000 description 2
- 241000187482 Mycobacterium avium subsp. paratuberculosis Species 0.000 description 2
- 241000187484 Mycobacterium gordonae Species 0.000 description 2
- 241000187490 Mycobacterium scrofulaceum Species 0.000 description 2
- 241001302239 Mycobacterium tuberculosis complex Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 231100000676 disease causative agent Toxicity 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 210000003296 saliva Anatomy 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 241001379910 Ephemera danica Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940072185 drug for treatment of tuberculosis Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9007192A FR2663033B1 (fr) | 1990-06-08 | 1990-06-08 | Detection specifique du mycobacterium tuberculosis. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69121975D1 DE69121975D1 (de) | 1996-10-17 |
| DE69121975T2 true DE69121975T2 (de) | 1997-03-27 |
Family
ID=9397443
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69121975T Expired - Lifetime DE69121975T2 (de) | 1990-06-08 | 1991-06-07 | Spezifischer Nachweis von Mycobacterium Tuberculosis |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0461045B1 (enExample) |
| JP (1) | JPH05507617A (enExample) |
| AT (1) | ATE142702T1 (enExample) |
| CA (1) | CA2084894A1 (enExample) |
| DE (1) | DE69121975T2 (enExample) |
| DK (1) | DK0461045T3 (enExample) |
| ES (1) | ES2093688T3 (enExample) |
| FR (1) | FR2663033B1 (enExample) |
| GR (1) | GR3021781T3 (enExample) |
| HK (1) | HK1000072A1 (enExample) |
| WO (1) | WO1991019004A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
| US7009041B1 (en) | 1989-07-11 | 2006-03-07 | Gen-Probe Incorporated | Oligonucleotides for nucleic acid amplification and for the detection of Mycobacterium tuberculosis |
| US5776693A (en) * | 1990-06-08 | 1998-07-07 | Institut Pasteur | Specific detection of the mycobacterium tuberculosis |
| AU666200B2 (en) * | 1992-04-28 | 1996-02-01 | Gen-Probe Incorporated | Nucleic acid process probes to (mycobacterium tuberculosis) |
| CA2135073C (en) * | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| GB9301776D0 (en) * | 1993-01-29 | 1993-03-17 | Univ Alberta | Method for detecting genomic variation |
| CA2163393C (en) * | 1994-11-30 | 2003-04-22 | Colleen Marie Nycz | Amplification and detection of mycobacteria nucleic acids |
| US5925518A (en) * | 1995-05-19 | 1999-07-20 | Akzo Nobel N.V. | Nucleic acid primers for amplification of a mycobacteria RNA template |
| US5731150A (en) * | 1995-11-01 | 1998-03-24 | Chiron Diagnostic Corporation | IS6110 based molecular detection of mycobacterium tuberculosis |
| FR2767337B1 (fr) | 1997-08-14 | 2002-07-05 | Pasteur Institut | Sequences nucleiques de polypeptides exportes de mycobacteri es, vecteurs les comprenant et applications au diagnostic et a la prevention de la tuberculose |
| FR2791067B1 (fr) * | 1999-03-16 | 2003-01-31 | Pasteur Institut | Sequence deletees chez m.bovis bcg/m.ou m.tuberculosis, procede de detection des mycobacteries utilisant ces sequences et vaccins |
| JP4092201B2 (ja) * | 2000-12-26 | 2008-05-28 | タカラバイオ株式会社 | 病原微生物の検出方法 |
| ES2560433T3 (es) | 2004-04-26 | 2016-02-19 | Wako Pure Chemical Industries, Ltd. | Cebadores para la detección del bacilo de la tuberculosis y procedimiento de detección del bacilo de la tuberculosis humana con los mismos |
| CA2621622A1 (en) | 2005-09-05 | 2007-03-15 | Bio-Rad Pasteur | Use of both rd9 and is6110 as nucleic acid targets for the diagnosis of tuberculosis, and provision of multiplex-compliant is6110 and rd9 targets |
| US20120100545A1 (en) * | 2009-07-03 | 2012-04-26 | Tan Tock Seng Hospital | Method and/or primers for the detection of mycobacterium tuberculosis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0460041B2 (en) * | 1989-02-22 | 2004-08-25 | Cogent Limited | Probes, kits and methods for the detection and differentiation of mycobacteria |
| FR2651505B1 (fr) * | 1989-09-06 | 1994-07-22 | Pasteur Institut | Fragments d'acides nucleiques derives d'un genome de mycobacterie appropriee, leurs applications dans le diagnostic des infections a mycobacteries et plasmides contenant lesdits fragments. |
-
1990
- 1990-06-08 FR FR9007192A patent/FR2663033B1/fr not_active Expired - Lifetime
-
1991
- 1991-06-07 JP JP91510731A patent/JPH05507617A/ja active Pending
- 1991-06-07 WO PCT/FR1991/000457 patent/WO1991019004A1/fr not_active Ceased
- 1991-06-07 EP EP91401501A patent/EP0461045B1/fr not_active Expired - Lifetime
- 1991-06-07 ES ES91401501T patent/ES2093688T3/es not_active Expired - Lifetime
- 1991-06-07 DE DE69121975T patent/DE69121975T2/de not_active Expired - Lifetime
- 1991-06-07 AT AT91401501T patent/ATE142702T1/de not_active IP Right Cessation
- 1991-06-07 DK DK91401501.1T patent/DK0461045T3/da active
- 1991-06-07 CA CA002084894A patent/CA2084894A1/fr not_active Abandoned
-
1996
- 1996-11-27 GR GR960403179T patent/GR3021781T3/el unknown
-
1997
- 1997-07-26 HK HK97101606A patent/HK1000072A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE142702T1 (de) | 1996-09-15 |
| HK1000072A1 (en) | 1997-11-14 |
| DE69121975D1 (de) | 1996-10-17 |
| DK0461045T3 (enExample) | 1997-02-17 |
| EP0461045A1 (fr) | 1991-12-11 |
| GR3021781T3 (en) | 1997-02-28 |
| FR2663033A1 (fr) | 1991-12-13 |
| EP0461045B1 (fr) | 1996-09-11 |
| WO1991019004A1 (fr) | 1991-12-12 |
| FR2663033B1 (fr) | 1992-09-04 |
| CA2084894A1 (fr) | 1991-12-09 |
| ES2093688T3 (es) | 1997-01-01 |
| JPH05507617A (ja) | 1993-11-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |