DE69029740D1 - Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe - Google Patents

Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe

Info

Publication number
DE69029740D1
DE69029740D1 DE69029740T DE69029740T DE69029740D1 DE 69029740 D1 DE69029740 D1 DE 69029740D1 DE 69029740 T DE69029740 T DE 69029740T DE 69029740 T DE69029740 T DE 69029740T DE 69029740 D1 DE69029740 D1 DE 69029740D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid sequence
specific nucleic
detecting
new method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69029740T
Other languages
English (en)
Other versions
DE69029740T2 (de
Inventor
Asa Frostell
Michael Nunn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia Biosystems AB
Cytiva Sweden AB
Original Assignee
Pharmacia Biosystems AB
Pharmacia Biotech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biosystems AB, Pharmacia Biotech AB filed Critical Pharmacia Biosystems AB
Publication of DE69029740D1 publication Critical patent/DE69029740D1/de
Application granted granted Critical
Publication of DE69029740T2 publication Critical patent/DE69029740T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69029740T 1989-11-30 1990-11-29 Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe Expired - Fee Related DE69029740T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US44391089A 1989-11-30 1989-11-30
US50583390A 1990-04-06 1990-04-06
US54802790A 1990-07-05 1990-07-05
PCT/US1990/006953 WO1991008308A1 (en) 1989-11-30 1990-11-29 A new method for detecting a specific nucleic acid sequence in a sample of cells

Publications (2)

Publication Number Publication Date
DE69029740D1 true DE69029740D1 (de) 1997-02-27
DE69029740T2 DE69029740T2 (de) 1997-07-10

Family

ID=27412193

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69029740T Expired - Fee Related DE69029740T2 (de) 1989-11-30 1990-11-29 Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe

Country Status (5)

Country Link
EP (1) EP0504278B1 (de)
JP (1) JPH05504475A (de)
AT (1) ATE147792T1 (de)
DE (1) DE69029740T2 (de)
WO (1) WO1991008308A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
NL9002696A (nl) * 1990-11-15 1992-06-01 U Gene Research Bv Werkwijze en kit voor het aantonen van microoerganismen.
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process
SE9201929D0 (sv) * 1992-06-23 1992-06-23 Pharmacia Lkb Biotech Method and system for molecular-biological diagnostics
AU686616B2 (en) * 1993-03-26 1998-02-12 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
AU4066997A (en) * 1996-08-16 1998-03-06 Pharmacia Biotech Inc. Device and methods for remotely induced thermal transduction in chemical and biochemical reactions
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
US5962665A (en) 1997-06-16 1999-10-05 Abbott Laboratories Nucleic acid primers and probes for detecting HIV-1 and HIV-2
US6001558A (en) * 1997-06-25 1999-12-14 Ortho Clinical Diagnostics, Inc. Amplification and detection of HIV-1 and/or HIV 2
EP1002138B1 (de) 1997-08-08 2004-11-17 Organon Teknika B.V. Nukleinsäuresequenzen als primer und sonden zur amplifizierung und erkennung von allen hiv-1 subtypen
ATE456677T1 (de) 1999-07-09 2010-02-15 Gen Probe Inc Hiv-1 detektion mittels nukleinsäureamplifizierung
GB0013658D0 (en) * 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
WO2005061738A1 (en) 2003-12-19 2005-07-07 Gen-Probe Incorporated Compositions, methods and kits for detecting the nucleic acids of hiv-1 and hiv-2
CN1910289B (zh) 2004-01-07 2012-05-23 日立化成研究中心公司 用于检测hiv的引物和探针
DK2730660T3 (en) * 2012-11-09 2015-08-24 Inst Rech Pour Le Développement Ird Tools and method for the detection and quantification of genetically different HIV-1, SIV and SIVgor viruses
CN103865921B (zh) * 2014-02-27 2016-01-20 中国水产科学研究院珠江水产研究所 一种利用趾甲提取dna扩增三线闭壳龟线粒体全长的方法
EP3149203B1 (de) * 2014-05-27 2019-12-04 Université Paris Descartes In-vitro-verfahren zur erkennung und quantifizierung von hiv-2
BR102014024905A2 (pt) * 2014-10-06 2016-04-12 Fundação Hemoct De Ribeirão Preto oligonucleotídeos, conjunto de oligonucleotídeos, kit para diagnóstico e discriminação de infecção por htlv-1/2, polinucleotídeo adequado para uso como alvo de referência para o desenho de iniciadores e sondas para detecção e diferenciação de htlv-1 e htlv-2, amplicon, e, método para detecção de pelo menos um alvo de htlv
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8311905D0 (en) * 1983-04-29 1983-06-02 Cox R A Isolation and detection of nucleotide sequence
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4952499A (en) * 1988-02-11 1990-08-28 Dana-Farber Cancer Institute Genes and their encoded proteins which regulate gene expression of the interleukin-2 receptor and of human lymphotropic retroviruses
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction

Also Published As

Publication number Publication date
ATE147792T1 (de) 1997-02-15
WO1991008308A1 (en) 1991-06-13
EP0504278A1 (de) 1992-09-23
JPH05504475A (ja) 1993-07-15
EP0504278B1 (de) 1997-01-15
DE69029740T2 (de) 1997-07-10
EP0504278A4 (en) 1993-06-09

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Legal Events

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8339 Ceased/non-payment of the annual fee