DE69008172D1 - Kartoffel-alpha-amylase-gene. - Google Patents

Kartoffel-alpha-amylase-gene.

Info

Publication number
DE69008172D1
DE69008172D1 DE69008172T DE69008172T DE69008172D1 DE 69008172 D1 DE69008172 D1 DE 69008172D1 DE 69008172 T DE69008172 T DE 69008172T DE 69008172 T DE69008172 T DE 69008172T DE 69008172 D1 DE69008172 D1 DE 69008172D1
Authority
DE
Germany
Prior art keywords
alpha
amylase
potato
alpha amylase
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69008172T
Other languages
English (en)
Other versions
DE69008172T2 (de
Inventor
Kirsten Gausing
Jette D Kreiberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DuPont Nutrition Biosciences ApS
Original Assignee
Danske Spritfabrikker AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danske Spritfabrikker AS filed Critical Danske Spritfabrikker AS
Application granted granted Critical
Publication of DE69008172D1 publication Critical patent/DE69008172D1/de
Publication of DE69008172T2 publication Critical patent/DE69008172T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2422Alpha-amylase (3.2.1.1.) from plant source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69008172T 1989-04-24 1990-04-24 Kartoffel-alpha-amylase-gene. Expired - Fee Related DE69008172T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK198089A DK198089D0 (da) 1989-04-24 1989-04-24 Dna-materialer og anvendelse deraf
PCT/DK1990/000108 WO1990012876A1 (en) 1989-04-24 1990-04-24 POTATO α-AMYLASE GENES

Publications (2)

Publication Number Publication Date
DE69008172D1 true DE69008172D1 (de) 1994-05-19
DE69008172T2 DE69008172T2 (de) 1994-10-20

Family

ID=8109247

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69008172T Expired - Fee Related DE69008172T2 (de) 1989-04-24 1990-04-24 Kartoffel-alpha-amylase-gene.

Country Status (12)

Country Link
US (1) US6147277A (de)
EP (1) EP0470145B1 (de)
AT (1) ATE104351T1 (de)
AU (1) AU638248B2 (de)
CA (1) CA2053230C (de)
DD (1) DD298949A5 (de)
DE (1) DE69008172T2 (de)
DK (2) DK198089D0 (de)
ES (1) ES2053189T3 (de)
IE (1) IE63067B1 (de)
LV (1) LV10727B (de)
WO (1) WO1990012876A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU644619B2 (en) * 1989-12-21 1993-12-16 Advanced Technologies (Cambridge) Limited Modification of plant metabolism
US7033627B2 (en) * 1990-03-23 2006-04-25 Syngenta Mogen B.V. Production of enzymes in seeds and their use
DE4013144A1 (de) * 1990-04-20 1991-10-24 Inst Genbiologische Forschung Neue plasmide, enthaltend dna-sequenzen, die veraenderungen der karbohydrat- und proteinkonzentration und der karbohydrat- und proteinzusammensetzung in kartoffelknollen hervorrufen, sowie zellen einer kartoffelpflanze, enthaltend diese plasmide
DK162790D0 (da) * 1990-07-06 1990-07-06 Novo Nordisk As Plantecelle
IE913215A1 (en) * 1990-09-13 1992-02-25 Gist Brocades Nv Transgenic plants having a modified carbohydrate content
US5705375A (en) * 1990-09-13 1998-01-06 Mogen International, N.V. Transgenic plants having a modified carbohydrate content
DE4035756A1 (de) * 1990-11-08 1992-05-14 Inst Genbiologische Forschung Neue plasmide zur herstellung von im habitus und ertrag veraenderten transgenen pflanzen
DE4104782B4 (de) * 1991-02-13 2006-05-11 Bayer Cropscience Gmbh Neue Plasmide, enthaltend DNA-Sequenzen, die Veränderungen der Karbohydratkonzentration und Karbohydratzusammensetzung in Pflanzen hervorrufen, sowie Pflanzen und Pflanzenzellen enthaltend dieses Plasmide
US5750876A (en) * 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
GB9421287D0 (en) * 1994-10-21 1994-12-07 Danisco A method of reducing the level of sugar in an organism
GB9421286D0 (en) 1994-10-21 1994-12-07 Danisco Promoter
GB9421292D0 (en) * 1994-10-21 1994-12-07 Danisco Promoter
WO1996031612A2 (en) * 1995-04-06 1996-10-10 Seminis Vegetables Process for selection of transgenic plant cells
GB9522589D0 (en) * 1995-11-03 1996-01-03 Innes John Centre Modified plants and plant products
DE69634875T2 (de) * 1995-12-27 2006-04-27 Japan Tobacco Inc. Kälteindusierbare promotorsequenzen
GB9526613D0 (en) * 1995-12-28 1996-02-28 Scottish Crop Research Inst Sequence
CN1246894A (zh) * 1997-02-10 2000-03-08 加拿大农业及农业食品部 具有降低的α葡聚糖L-或H-型块茎磷酸化酶活性水平的低冷甜化转基因马铃薯
US5998701A (en) * 1997-06-04 1999-12-07 Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture Potatoes having improved quality characteristics and methods for their production
CA2280230A1 (en) 1997-02-21 1998-08-27 Danisco A/S Antisense intron inhibition of starch branching enzyme expression
GB9710990D0 (en) * 1997-05-28 1997-07-23 Danisco Modification process
TW508860B (en) * 2000-08-30 2002-11-01 Mitsui & Amp Co Ltd Paste-like thin electrode for battery, its manufacturing method, and battery
US7612251B2 (en) * 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
DE60234523D1 (de) 2001-05-15 2010-01-07 Novozymes As Alpha-amylasevariante mit veränderten eigenschaften
DE60238339D1 (de) 2001-08-27 2010-12-30 Syngenta Participations Ag Selbstprozessierende pflanzen und pflanzenteile
NZ514547A (en) * 2001-09-28 2004-10-29 Duncan Stanley Plastid alph-amylase protein and nucleic acids encoding same and methods for altering the starch content of a plant
EP1840211A1 (de) 2001-10-31 2007-10-03 Danisco A/S Pyranoson Dehydratase aus phanerochaete Chrysosporium
EP1730284A4 (de) * 2004-03-08 2008-04-30 Syngenta Participations Ag Selbstprozessierende pflanzen und pflanzenteile
PT1868426T (pt) * 2005-03-16 2018-05-08 Syngenta Participations Ag Evento de milho 3272 e métodos para a sua deteção
US7915020B2 (en) * 2007-06-01 2011-03-29 Syngenta Participations Ag Process for starch liquefaction and fermentation
US7727726B2 (en) * 2007-06-01 2010-06-01 Syngenta Participations Ag Process for starch liquefaction and fermentation
WO2008150880A1 (en) * 2007-06-01 2008-12-11 Syngenta Participations Ag Process for starch liquefaction and fermentation
JP5695422B2 (ja) * 2007-11-27 2015-04-08 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション デンプン代謝改変植物
KR20140056237A (ko) 2011-06-30 2014-05-09 노보자임스 에이/에스 알파-아밀라제 변이체

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4794175A (en) * 1983-12-20 1988-12-27 Cetus Corporation Glucoamylase CDNA
US4771002A (en) * 1984-02-24 1988-09-13 Lubrizol Genetics, Inc. Transcription in plants and bacteria
US4736866A (en) * 1984-06-22 1988-04-12 President And Fellows Of Harvard College Transgenic non-human mammals
US4769327A (en) * 1985-03-29 1988-09-06 Biotechnica International, Inc. Secretion vector
US5498832A (en) * 1989-04-24 1996-03-12 A/S De Danske Spritfabrikker Potato α-amylase genes

Also Published As

Publication number Publication date
DE69008172T2 (de) 1994-10-20
CA2053230A1 (en) 1990-10-25
ES2053189T3 (es) 1994-07-16
IE901450L (en) 1990-10-24
AU638248B2 (en) 1993-06-24
DK0470145T3 (da) 1994-08-08
LV10727B (en) 1996-02-20
WO1990012876A1 (en) 1990-11-01
US6147277A (en) 2000-11-14
CA2053230C (en) 2003-11-25
LV10727A (lv) 1995-06-20
ATE104351T1 (de) 1994-04-15
EP0470145A1 (de) 1992-02-12
EP0470145B1 (de) 1994-04-13
DK198089D0 (da) 1989-04-24
DD298949A5 (de) 1992-03-19
IE63067B1 (en) 1995-03-22
AU5531890A (en) 1990-11-16

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8339 Ceased/non-payment of the annual fee