DE663587C - Process for the enrichment and purification of an activator that increases the male sex hormone effect - Google Patents

Process for the enrichment and purification of an activator that increases the male sex hormone effect

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Publication number
DE663587C
DE663587C DEG91979D DEG0091979D DE663587C DE 663587 C DE663587 C DE 663587C DE G91979 D DEG91979 D DE G91979D DE G0091979 D DEG0091979 D DE G0091979D DE 663587 C DE663587 C DE 663587C
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DE
Germany
Prior art keywords
activator
enrichment
sex hormone
male sex
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEG91979D
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemische Ind Ges
GESELLSCHAFT fur CHEMISCHE INDUSTRIE
BASF Schweiz AG
Original Assignee
Chemische Ind Ges
GESELLSCHAFT fur CHEMISCHE INDUSTRIE
Gesellschaft fuer Chemische Industrie in Basel CIBA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemische Ind Ges, GESELLSCHAFT fur CHEMISCHE INDUSTRIE, Gesellschaft fuer Chemische Industrie in Basel CIBA filed Critical Chemische Ind Ges
Application granted granted Critical
Publication of DE663587C publication Critical patent/DE663587C/en
Expired legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Steroid Compounds (AREA)

Description

Verfahren zur Anreicherung und Reinigung eines die männliche Sexualhormonwirkung steigernden Aktivators Durch Arbeiten von L a q u e u r und Mitarbeiter (s. z. B. Zeitschi. für physiolog. Chemie, Bd. 233 [19351, S. 281; Acta brevia Neerlandica, Bd.5 [t935], S.84) wurde in Extrakten aus tierischen Organen, Körperflüssigkeiten oder auch aus pflanzlichem Material eine Verbindung nachgewiesen, die, obschon an und für sich unwirksam, die Wirkung gewisser männlicher Hormone, wie Testosteron, Dihydroandr osteron, z. B. auf die Samenblase sehr zu steigern vermag.. Es wurde nun gefunden, daß sich dieser Aktivator für männliche Hormone anreichern und reinigen läßt, wenn man aktivatorhaltige Präparate, insbesondere auch rolle oder vorgereinigte Extrakte aus tierischen Organen, menschlichen oder tierischen Körperflüssigkeiten oder pflanzlichem Material, einer Vakuumdestillation unterwirft. überraschenderweise gelingt es auf diesem Wege, den Aktivator ohne Einbuße seiner Wirksamkeit als farbloses Destillat aufzufangen und eine Reihe von Verunreinigungen, namentlich auch Pigmente, als Destillationsrückstand abzutrennen. Vorteilhafterweise wird die Vakuumdestillation fraktioniert durchgeführt; sie kann unmittelbar oder nach weiteren Reinigungsstufen wiederholt werden.Process for the enrichment and purification of one of the male sex hormone effects increasing activator Through the work of L a q u e u r and employees (see e.g. Zeitschi. for physiolog. Chemie, Vol. 233 [19351, p. 281; Acta brevia Neerlandica, Vol.5 [t935], p.84) was found in extracts from animal organs, body fluids or from plant material a compound has been demonstrated which, although at and ineffective in itself, the effects of certain male hormones, such as testosterone, Dihydroandr osteron, e.g. B. able to increase the seminal vesicle very much. It was now found that this activator for male hormones accumulate and cleanse leaves, if one activator-containing preparations, especially also roll or pre-cleaned Extracts from animal organs, human or animal body fluids or vegetable matter, subjected to vacuum distillation. surprisingly in this way it is possible to use the activator as a colorless one without losing its effectiveness To collect distillate and a number of impurities, including pigments, to be separated off as distillation residue. Vacuum distillation is advantageous carried out fractionally; it can be used immediately or after further cleaning stages be repeated.

Die Destillation sexualhormonhaltiger, insbesondere follikelhormonhaltiger Zubereitungen ist bereits bekannt (vgl. z. B. B u t e -n a n d t , Untersuchungen über das weibliche Sexualhormon, 1931, S.45 ff.). Daraus war aber das vorliegende Verfahren zur Reinigung und Anreicherung des an und für sich unwirksamen Aktivators in keiner Weise abzuleiten. überdies ist es überraschend, daß sich dieser Aktivator unzersetzt destillieren läßt. Beispiel Eine saure Fraktion aus Hoden, wie sie durch Ausziehen eines Hodehextraktes mit verdünntem Alkali, Ansäuern, Extraktion der sauren Bestandteile z. B. mit Äther und Vertreiben des Lösungsmittels erhalten werden kann, wird im Hochvakuum bei 0,0005 mm Quecksilber destilliert. Man fängt die bei 125 bis 14o° übergehende farblose Fraktion auf, die sich zu einer stearinartigen Masse verfestigt. Sie bewirkt, im Gegensatz zum dunkelrotbraunen, verharzten Destillationsrückstand, eine starke Steigerung der Wirksamkeit von gleichzeitig' injiziertem Testosteron auf die Samenblasen kastrierter Ratten.The distillation of preparations containing sex hormones, in particular preparations containing follicle hormones, is already known (cf., for example, Bute-nandt, Studies on the Female Sex Hormone, 1931, pp. 45 ff.). However, the present process for purifying and enriching the activator, which is in and of itself ineffective, could in no way be derived from this. In addition, it is surprising that this activator can be distilled without decomposition. Example An acidic fraction from testes, as obtained by extracting a testicle extract with dilute alkali, acidification, extraction of the acidic constituents e.g. B. can be obtained with ether and expelling the solvent, is distilled in a high vacuum at 0.0005 mm of mercury. The colorless fraction which passes over at 125 ° to 140 ° is collected and solidifies to form a stearic mass. In contrast to the dark red-brown, gummy distillation residue, it greatly increases the effectiveness of simultaneously injected testosterone on the seminal vesicles of castrated rats.

Claims (3)

PATRNTANSPRÜcIIR: i. Verfahren zur Anreicherung und 1 nigung eines die männliche Sexualhormo*nwirkung steigernden A-ktivators, dadurch g e 'keni' izeichnet, daß man aktivatorhalt; 401' e Präparate, insbesondere auch rohe oder vorgereinigte Extrakte aus tierischen 0rganen, menschlichen. oder tierischen Kürperflüssig'keiten oder pflanzlichem Material, einer Vakuumdestillation unterwirft. PATENT CLAIM: i. Procedure for the enrichment and cleaning of a activator increasing the male sex hormone effect, thereby g e 'keni' izeign, that one stops activator; 401'e preparations, in particular also raw or pre-cleaned Extracts from animal organs, human. or animal body fluids or vegetable matter, subjected to vacuum distillation. 2. Ausführungsform des Verfahrens nach Anspruch i, dadurch gekennzeichnet, daß man die Vakuumdestillation frakr tioniert ausführt. 2nd embodiment of the method according to claim i, characterized in that the vacuum distillation executes fractionally. 3. Ausführungsform des Verfahrens nach den Ansprüchen i und 2, dadurch gekennzeichnet, daß man die Vakuumdestillation unmittelbar oder nach weiteren Reinigungsstufen wiederholt.3. embodiment of the method according to claims i and 2, characterized in that the vacuum distillation immediately or after repeated further cleaning stages.
DEG91979D 1936-01-13 1936-01-21 Process for the enrichment and purification of an activator that increases the male sex hormone effect Expired DE663587C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH663587X 1936-01-13

Publications (1)

Publication Number Publication Date
DE663587C true DE663587C (en) 1938-08-09

Family

ID=4527104

Family Applications (1)

Application Number Title Priority Date Filing Date
DEG91979D Expired DE663587C (en) 1936-01-13 1936-01-21 Process for the enrichment and purification of an activator that increases the male sex hormone effect

Country Status (1)

Country Link
DE (1) DE663587C (en)

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