DE60318150D1 - Verfahren zur optischen Bestimmung von mehrsträngigen Nucleinsäuren mit Hilfe von Cyaninfarbstoffen - Google Patents
Verfahren zur optischen Bestimmung von mehrsträngigen Nucleinsäuren mit Hilfe von CyaninfarbstoffenInfo
- Publication number
- DE60318150D1 DE60318150D1 DE60318150T DE60318150T DE60318150D1 DE 60318150 D1 DE60318150 D1 DE 60318150D1 DE 60318150 T DE60318150 T DE 60318150T DE 60318150 T DE60318150 T DE 60318150T DE 60318150 D1 DE60318150 D1 DE 60318150D1
- Authority
- DE
- Germany
- Prior art keywords
- stranded nucleic
- nucleic acid
- compound
- aid
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 102000039446 nucleic acids Human genes 0.000 title abstract 7
- 108020004707 nucleic acids Proteins 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 2
- 230000003287 optical effect Effects 0.000 title abstract 2
- 239000000975 dye Substances 0.000 title 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 5
- 230000003993 interaction Effects 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/06—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups three >CH- groups, e.g. carbocyanines
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
- C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B69/00—Dyes not provided for by a single group of this subclass
- C09B69/001—Dyes containing an onium group attached to the dye skeleton via a bridge
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002036474A JP3899373B2 (ja) | 2002-02-14 | 2002-02-14 | 核酸染色用色素 |
JP2002036474 | 2002-02-14 | ||
JP2002036473 | 2002-02-14 | ||
JP2002036473A JP3899372B2 (ja) | 2002-02-14 | 2002-02-14 | 多重鎖核酸の光学的測定方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60318150D1 true DE60318150D1 (de) | 2008-01-31 |
DE60318150T2 DE60318150T2 (de) | 2008-12-04 |
Family
ID=29272299
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60318150T Expired - Lifetime DE60318150T2 (de) | 2002-02-14 | 2003-02-14 | Verfahren zur optischen Bestimmung von mehrsträngigen Nucleinsäuren mit Hilfe von Cyaninfarbstoffen |
Country Status (4)
Country | Link |
---|---|
US (2) | US20050112770A1 (de) |
EP (2) | EP1889880A3 (de) |
AT (1) | ATE381595T1 (de) |
DE (1) | DE60318150T2 (de) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE508116T1 (de) * | 2004-02-13 | 2011-05-15 | Dsm Ip Assets Bv | Ionische uv-a-sonnenschutzmittel und zusammensetzungen, die diese enthalten |
US7354720B2 (en) * | 2004-12-30 | 2008-04-08 | Affymetrix, Inc. | Label free analysis of nucleic acids |
EP2620508B1 (de) * | 2010-09-22 | 2017-09-06 | Kaneka Corporation | Verfahren und vorrichtung zum nachweis von nukleinsäure und kit dafür |
US9273174B2 (en) | 2011-03-04 | 2016-03-01 | Kaneka Corporation | Nucleic acid detection method, and device and kit for use in same |
US10260089B2 (en) | 2012-10-29 | 2019-04-16 | The Research Foundation Of The State University Of New York | Compositions and methods for recognition of RNA using triple helical peptide nucleic acids |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19818176A1 (de) * | 1998-04-23 | 1999-10-28 | Basf Ag | Verfahren zur Markierung von Flüssigkeiten mit mindestens zwei Markierstoffen und Verfahren zu deren Detektion |
DE518291C (de) | 1927-01-15 | 1931-02-18 | Schering Kahlbaum Ag | Verfahren zur Darstellung von 4-Alkylchinolinen und deren Derivaten |
GB870753A (en) * | 1957-07-04 | 1961-06-21 | Ilford Ltd | Improvements in or relating to methine dyes |
US4313951A (en) | 1979-11-26 | 1982-02-02 | Sterling Drug Inc. | 3-Substituted-6-(lower-alkyl)-5-(pyridinyl)-2(1H)-pyridinones, their cardiotonic use and intermediates therefor |
NZ196573A (en) | 1980-03-28 | 1984-03-16 | Sterling Drug Inc | 6-pyrid-(3 or 4)-yl-(1h or 3h)-imidazo (4,5-b)pyridines |
JP2573443B2 (ja) | 1990-09-28 | 1997-01-22 | 株式会社東芝 | 遺伝子検出法 |
US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
US5410030A (en) * | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
US5656449A (en) * | 1995-03-06 | 1997-08-12 | Molecular Probes, Inc. | Neutral unsymmetrical cyanine dyes |
US6664047B1 (en) * | 1999-04-30 | 2003-12-16 | Molecular Probes, Inc. | Aza-benzazolium containing cyanine dyes |
JP3342695B2 (ja) | 1999-12-06 | 2002-11-11 | 富士写真フイルム株式会社 | 反応性固相担体及びdna断片検出用具 |
JP2001163895A (ja) | 1999-12-07 | 2001-06-19 | Fuji Photo Film Co Ltd | 蛍光ヌクレオチド |
JP2001270864A (ja) | 2000-03-27 | 2001-10-02 | Fuji Photo Film Co Ltd | インドレニン誘導体の製造方法 |
JP2001270957A (ja) | 2000-03-27 | 2001-10-02 | Jsr Corp | スポンジ用ゴム組成物およびスポンジ |
-
2003
- 2003-02-14 EP EP07023423A patent/EP1889880A3/de not_active Withdrawn
- 2003-02-14 EP EP03003488A patent/EP1362894B1/de not_active Expired - Lifetime
- 2003-02-14 DE DE60318150T patent/DE60318150T2/de not_active Expired - Lifetime
- 2003-02-14 US US10/366,449 patent/US20050112770A1/en not_active Abandoned
- 2003-02-14 AT AT03003488T patent/ATE381595T1/de not_active IP Right Cessation
-
2007
- 2007-10-30 US US11/928,238 patent/US20080124732A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1889880A3 (de) | 2008-06-18 |
US20050112770A1 (en) | 2005-05-26 |
DE60318150T2 (de) | 2008-12-04 |
EP1362894B1 (de) | 2007-12-19 |
EP1362894A2 (de) | 2003-11-19 |
ATE381595T1 (de) | 2008-01-15 |
EP1362894A3 (de) | 2004-03-10 |
US20080124732A1 (en) | 2008-05-29 |
EP1889880A2 (de) | 2008-02-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |