DE60229805D1 - Nukleinsäurereinigung - Google Patents

Nukleinsäurereinigung

Info

Publication number
DE60229805D1
DE60229805D1 DE60229805T DE60229805T DE60229805D1 DE 60229805 D1 DE60229805 D1 DE 60229805D1 DE 60229805 T DE60229805 T DE 60229805T DE 60229805 T DE60229805 T DE 60229805T DE 60229805 D1 DE60229805 D1 DE 60229805D1
Authority
DE
Germany
Prior art keywords
nucleic acid
plasmid dna
concentration
retentate
acid solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60229805T
Other languages
English (en)
Inventor
Rimantas Kvederas
Asta Siksniute
Algimantas Markauskas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Fisher Scientific Baltics UAB
Original Assignee
Fermentas UAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fermentas UAB filed Critical Fermentas UAB
Application granted granted Critical
Publication of DE60229805D1 publication Critical patent/DE60229805D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE60229805T 2001-03-27 2002-03-27 Nukleinsäurereinigung Expired - Lifetime DE60229805D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0107634.8A GB0107634D0 (en) 2001-03-27 2001-03-27 Nucleic acid purification

Publications (1)

Publication Number Publication Date
DE60229805D1 true DE60229805D1 (de) 2008-12-24

Family

ID=9911655

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60229805T Expired - Lifetime DE60229805D1 (de) 2001-03-27 2002-03-27 Nukleinsäurereinigung

Country Status (9)

Country Link
US (1) US7811807B2 (de)
EP (1) EP1245674B9 (de)
AT (1) ATE414147T1 (de)
DE (1) DE60229805D1 (de)
DK (1) DK1245674T3 (de)
EE (1) EE05203B1 (de)
ES (1) ES2315340T3 (de)
GB (1) GB0107634D0 (de)
PT (1) PT1245674E (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113186185A (zh) * 2020-01-14 2021-07-30 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60325773D1 (de) * 2002-12-23 2009-02-26 Vical Inc Reinigungsverfahren für plasmid-dna
CA2668818C (en) 2006-10-10 2018-06-26 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
EP3399036B1 (de) 2010-04-08 2023-01-25 QIAGEN GmbH Chromatografische vorrichtung und verfahren zur isolierung und reinigung von nukleinsäuren
JP2013523143A (ja) * 2010-04-08 2013-06-17 キアゲン ゲーエムベーハー 核酸を単離および精製するための方法
US20130023655A1 (en) * 2010-04-08 2013-01-24 Qiagen Gmbh Method for precipitating anionic surfactant ions in the presence of nucleic acids
EP2395082A1 (de) 2010-06-14 2011-12-14 QIAGEN GmbH Extraktion von Nukleinsäuren aus in Wachs eingebetteten Proben
DK3294885T3 (da) 2015-05-08 2020-08-10 Curevac Real Estate Gmbh Fremgangsmåde til at fremstille rna
CN107873055B (zh) 2015-05-29 2021-09-17 库瑞瓦格房地产有限公司 包括至少一个切向流过滤步骤的产生和纯化rna的方法
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN114907430B (zh) * 2022-06-21 2023-12-01 中国科学院过程工程研究所 一种mRNA的分离纯化方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
US4861718A (en) * 1984-09-29 1989-08-29 Wakamoto Pharmaceutical Co., Ltd. Gene coding for thermostable beta-galactosidase, bacillus subtilis having the gene, enzyme coded by the gene and a process for the production thereof
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
US5256294A (en) * 1990-09-17 1993-10-26 Genentech, Inc. Tangential flow filtration process and apparatus
JPH04360686A (ja) * 1991-06-04 1992-12-14 Tosoh Corp Ｄｎａの精製方法
AR003122A1 (es) * 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
AU7701398A (en) 1997-05-30 1998-12-30 Bion, Inc. Large-scale isolation and purification of hif
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
US6441160B2 (en) * 1998-05-11 2002-08-27 Tosoh Corporation Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography
NZ515645A (en) * 1999-05-28 2004-07-30 Cambrex Bio Science Baltimore Methods of purifying plasmid DNA mixture containing host cell impurities
JP2003505077A (ja) * 1999-07-23 2003-02-12 ジェネンテック・インコーポレーテッド 平行流ろ過法を用いるｒｎａｓｅ−及び有機溶媒−非含有プラスミドｄｎａの精製方法
US6350382B1 (en) * 2000-06-23 2002-02-26 Scilog, Inc. Enhancing filtration yields in tangential flow filtration
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113186185A (zh) * 2020-01-14 2021-07-30 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法
CN113186185B (zh) * 2020-01-14 2023-05-26 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法

Also Published As

Publication number Publication date
PT1245674E (pt) 2008-12-23
US20030109696A1 (en) 2003-06-12
EE05203B1 (et) 2009-08-17
DK1245674T3 (da) 2009-01-19
EP1245674A3 (de) 2003-12-17
EP1245674B1 (de) 2008-11-12
EE200200164A (et) 2002-12-16
GB0107634D0 (en) 2001-05-16
EP1245674B9 (de) 2009-10-21
ATE414147T1 (de) 2008-11-15
US7811807B2 (en) 2010-10-12
ES2315340T3 (es) 2009-04-01
EP1245674A2 (de) 2002-10-02

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