DE60228847D1 - - Google Patents

Info

Publication number
DE60228847D1
DE60228847D1 DE60228847T DE60228847T DE60228847D1 DE 60228847 D1 DE60228847 D1 DE 60228847D1 DE 60228847 T DE60228847 T DE 60228847T DE 60228847 T DE60228847 T DE 60228847T DE 60228847 D1 DE60228847 D1 DE 60228847D1
Authority
DE
Germany
Prior art keywords
rna
cellular
cell
protein synthesis
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60228847T
Other languages
German (de)
English (en)
Inventor
Vehary Sakanyan
Marina Snapyan
Anahit Ghochikyan
Francoise-Michele Lecocq
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nantes Université
Original Assignee
Universite de Nantes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Nantes filed Critical Universite de Nantes
Application granted granted Critical
Publication of DE60228847D1 publication Critical patent/DE60228847D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60228847T 2001-07-27 2002-07-26 Expired - Lifetime DE60228847D1 (https=)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01402049A EP1279736A1 (en) 2001-07-27 2001-07-27 Methods of RNA and protein synthesis
PCT/EP2002/009423 WO2003012114A2 (en) 2001-07-27 2002-07-26 Methods of rna and protein synthesis

Publications (1)

Publication Number Publication Date
DE60228847D1 true DE60228847D1 (https=) 2008-10-23

Family

ID=8182831

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60228847T Expired - Lifetime DE60228847D1 (https=) 2001-07-27 2002-07-26

Country Status (6)

Country Link
US (1) US7186525B2 (https=)
EP (2) EP1279736A1 (https=)
AT (1) ATE408017T1 (https=)
AU (1) AU2002329256A1 (https=)
DE (1) DE60228847D1 (https=)
WO (1) WO2003012114A2 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100447530B1 (ko) * 2001-08-14 2004-09-08 한국과학기술원 ＯｍｐＦ를 이용하여 목적 단백질을 대장균 세포외로분비생산하는 방법
US20060084136A1 (en) * 2004-07-14 2006-04-20 Invitrogen Corporation Production of fusion proteins by cell-free protein synthesis
US20080248565A1 (en) * 2007-03-01 2008-10-09 Invitrogen Corporation Isolated phospholipid-protein particles
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
WO2007037301A1 (ja) * 2005-09-29 2007-04-05 Kyowa Hakko Kogyo Co., Ltd. 有用物質の製造法
EP3121286B1 (en) 2006-12-21 2019-11-20 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2449132B1 (en) 2009-07-01 2015-05-13 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
CA2809284C (en) 2010-08-31 2021-02-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
DK2890814T3 (da) 2012-08-30 2019-12-16 Gen Probe Inc Flerfaset nukleinsyreamplificering
BR112016002494A2 (pt) 2013-08-05 2017-09-05 Greenlight Biosciences Inc Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes
WO2016005982A1 (en) * 2014-07-08 2016-01-14 Technion Research & Development Foundation Limited Methods and kits for cell-free transcription and translation
KR20180002636A (ko) 2015-03-30 2018-01-08 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
CN107849573B (zh) * 2015-04-30 2023-04-14 恩格内斯生物科技有限公司 生长和蛋白质生产的解偶联
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
US12227778B2 (en) 2016-12-30 2025-02-18 Nature's Toolbox, Inc. Cell-free expression system having novel inorganic polyphosphate-based energy regeneration
WO2018126287A1 (en) * 2016-12-30 2018-07-05 Ntxbio, Llc Cell-free expression system having novel inorganic polyphosphate-based energy regeneration
US20200181670A1 (en) * 2017-08-11 2020-06-11 Synvitrobio, Inc. Improved In Vitro Transcription/Translation (TXTL) System and Use Thereof
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
US11673921B2 (en) 2017-11-10 2023-06-13 Northwestern University Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens
US11814621B2 (en) 2018-06-01 2023-11-14 Northwestern University Expanding the chemical substrates for genetic code reprogramming
WO2020010356A1 (en) 2018-07-06 2020-01-09 Northwestern University Ribosome variants for sequence defined polymer synthesis
US12098433B2 (en) 2018-08-03 2024-09-24 Northwestern University On demand, portable, cell-free molecular sensing platform
CA3131243A1 (en) 2019-02-25 2020-09-03 Northwestern University Cell-free protein synthesis platforms derived from clostridia
KR20210151928A (ko) * 2019-04-12 2021-12-14 네이쳐스 툴박스 인코포레이티드 호열성 단백질을 이용한 재조합 시험관내 전사 및 해독을 위한 시스템, 방법 및 조성물
BR112022002365A2 (pt) 2019-08-09 2022-04-26 Nutcracker Therapeutics Inc Métodos e aparelhos para fabricar e para remover material de uma composição terapêutica
WO2024120767A1 (en) * 2022-12-05 2024-06-13 Novozymes A/S Modified rna polymerase activities

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7625298A (en) * 1996-12-03 1998-06-29 Scriptgen Pharmaceuticals, Inc. M. tuberculosis RNA polymerase alpha subunit
AU4241400A (en) * 1999-04-16 2000-11-02 Board Of Trustees Of The University Of Arkansas, The Agrobacterium tumefaciens rpoa gene

Also Published As

Publication number Publication date
US7186525B2 (en) 2007-03-06
AU2002329256A1 (en) 2003-02-17
US20050032086A1 (en) 2005-02-10
ATE408017T1 (de) 2008-09-15
WO2003012114A2 (en) 2003-02-13
WO2003012114A3 (en) 2003-08-28
EP1279736A1 (en) 2003-01-29
EP1412504A2 (en) 2004-04-28
EP1412504B1 (en) 2008-09-10

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Legal Events

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8364 No opposition during term of opposition