DE60207995D1 - Verfahren zum entschützen von oligonukleotide - Google Patents

Verfahren zum entschützen von oligonukleotide

Info

Publication number
DE60207995D1
DE60207995D1 DE60207995T DE60207995T DE60207995D1 DE 60207995 D1 DE60207995 D1 DE 60207995D1 DE 60207995 T DE60207995 T DE 60207995T DE 60207995 T DE60207995 T DE 60207995T DE 60207995 D1 DE60207995 D1 DE 60207995D1
Authority
DE
Germany
Prior art keywords
reagents
deprotection
electron
withdrawing group
active methylene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60207995T
Other languages
English (en)
Other versions
DE60207995T2 (de
Inventor
Jeffrey Nelson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Publication of DE60207995D1 publication Critical patent/DE60207995D1/de
Application granted granted Critical
Publication of DE60207995T2 publication Critical patent/DE60207995T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60207995T 2001-03-08 2002-03-04 Verfahren zum entschützen von oligonukleotiden Expired - Fee Related DE60207995T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27430901P 2001-03-08 2001-03-08
US274309P 2001-03-08
PCT/US2002/006739 WO2002072864A2 (en) 2001-03-08 2002-03-04 Reagents for oligonucleotide cleavage and deprotection

Publications (2)

Publication Number Publication Date
DE60207995D1 true DE60207995D1 (de) 2006-01-19
DE60207995T2 DE60207995T2 (de) 2006-08-03

Family

ID=23047660

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60207995T Expired - Fee Related DE60207995T2 (de) 2001-03-08 2002-03-04 Verfahren zum entschützen von oligonukleotiden

Country Status (7)

Country Link
US (2) US6664388B2 (de)
EP (1) EP1366057B1 (de)
JP (1) JP2004521628A (de)
AT (1) ATE312841T1 (de)
CA (1) CA2468425A1 (de)
DE (1) DE60207995T2 (de)
WO (1) WO2002072864A2 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
US6593464B1 (en) * 1999-05-24 2003-07-15 Invitrogen Corporation Method for deblocking of labeled oligonucleotides
US7345163B2 (en) * 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
US20040180368A1 (en) * 2002-12-23 2004-09-16 Affymetrix, Inc. Method for producing a high density nucleic acid array using activators
CA2561741C (en) * 2004-04-05 2016-09-27 Alnylam Pharmaceuticals, Inc. Processes and reagents for oligonucleotide synthesis and purification
ATE400579T1 (de) * 2004-04-19 2008-07-15 Avecia Biotechnology Inc Verfahren zum abspalten exocyclischer basenschutzgruppen
US20080213902A1 (en) * 2006-08-04 2008-09-04 Ikonisys, Inc. Slide Holder for Staining Procedures
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US20080078535A1 (en) * 2006-10-03 2008-04-03 General Electric Company Heat exchanger tube with enhanced heat transfer co-efficient and related method
EP2276775B1 (de) * 2008-04-24 2015-09-02 Nitto Denko Avecia Inc. Verfahren zur herstellung von oligonukleotiden
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
ES2574625T3 (es) * 2009-11-25 2016-06-21 Elitechgroup B.V. Antagonistas de miARN de oligonucleótido conjugado con aglutinante del surco menor (MGB)
EP3085791A1 (de) 2009-11-25 2016-10-26 Gen9, Inc. Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US9295965B2 (en) 2010-11-12 2016-03-29 Gen9, Inc. Methods and devices for nucleic acid synthesis
EP2637780B1 (de) 2010-11-12 2022-02-09 Gen9, Inc. Proteinanordnungen und verfahren zu ihrer herstellung und verwendung
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US20130059296A1 (en) 2011-08-26 2013-03-07 Gen9, Inc. Compositions and Methods For High Fidelity Assembly of Nucleic Acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20150191719A1 (en) 2012-06-25 2015-07-09 Gen9, Inc. Methods for Nucleic Acid Assembly and High Throughput Sequencing
TWI646230B (zh) 2013-08-05 2019-01-01 扭轉生物科技有限公司 重新合成之基因庫
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
JP6982362B2 (ja) 2015-09-18 2021-12-17 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
CN113604546A (zh) 2015-09-22 2021-11-05 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN110248724B (zh) 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
US11634534B2 (en) * 2016-12-15 2023-04-25 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Degradable silyl-containing thermosetting networks
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
US10894242B2 (en) * 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物
CN112639130B (zh) 2018-05-18 2024-08-09 特韦斯特生物科学公司 用于核酸杂交的多核苷酸、试剂和方法
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
SG11202109322TA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for glp1 receptor
KR20220066151A (ko) 2019-09-23 2022-05-23 트위스트 바이오사이언스 코포레이션 Crth2에 대한 변이체 핵산 라이브러리
GB2626789A (en) * 2023-02-03 2024-08-07 Exactmer Ltd Deprotection processes and cation scavengers for use in the same

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965349A (en) 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5656741A (en) 1992-03-30 1997-08-12 Barrskogen, Inc. Process and reagents for processing synthetic oligonucleotides
US5348868A (en) * 1992-04-24 1994-09-20 Beckman Instruments, Inc. Methods and reagents for cleaving and deprotecting oligonucleotides
US5476925A (en) * 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
WO1996003417A1 (en) 1994-07-25 1996-02-08 Hybridon, Inc. Improved methods of detritylation for oligonucleotide synthesis
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
DE19627898A1 (de) 1996-07-11 1998-01-15 Hoechst Ag Festphasensynthese von Oligonucleotiden
EP0839829A3 (de) 1996-09-06 1998-10-07 Beckman Coulter, Inc. Universeller Festträger oligonukleotiden Reagenzion
US6465628B1 (en) 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
EP1149109B1 (de) 1999-02-05 2009-09-16 Amersham Biosciences Corp. Verfahren zum entschützen von oligonukleotiden

Also Published As

Publication number Publication date
EP1366057A2 (de) 2003-12-03
US20030181712A1 (en) 2003-09-25
EP1366057A4 (de) 2004-04-14
JP2004521628A (ja) 2004-07-22
EP1366057B1 (de) 2005-12-14
US20040191808A1 (en) 2004-09-30
WO2002072864A2 (en) 2002-09-19
CA2468425A1 (en) 2003-09-19
DE60207995T2 (de) 2006-08-03
ATE312841T1 (de) 2005-12-15
WO2002072864A3 (en) 2002-12-27
US6664388B2 (en) 2003-12-16

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee