DE60201441D1 - Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation - Google Patents

Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation

Info

Publication number
DE60201441D1
DE60201441D1 DE60201441T DE60201441T DE60201441D1 DE 60201441 D1 DE60201441 D1 DE 60201441D1 DE 60201441 T DE60201441 T DE 60201441T DE 60201441 T DE60201441 T DE 60201441T DE 60201441 D1 DE60201441 D1 DE 60201441D1
Authority
DE
Germany
Prior art keywords
oligonucleotide
genomic dna
pair
polarisation
fluorescent label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60201441T
Other languages
English (en)
Other versions
DE60201441T2 (de
Inventor
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to DE60201441T priority Critical patent/DE60201441T2/de
Application granted granted Critical
Publication of DE60201441D1 publication Critical patent/DE60201441D1/de
Publication of DE60201441T2 publication Critical patent/DE60201441T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60201441T 2001-01-29 2002-01-29 Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation Expired - Lifetime DE60201441T2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE60201441T DE60201441T2 (de) 2001-01-29 2002-01-29 Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10104937A DE10104937B4 (de) 2001-01-29 2001-01-29 Fluoreszenzpolarisation 2
DE10104937 2001-01-29
DE60201441T DE60201441T2 (de) 2001-01-29 2002-01-29 Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation
PCT/EP2002/000922 WO2002061123A2 (en) 2001-01-29 2002-01-29 Method of analysing dna methylation using fluorescence polarisation

Publications (2)

Publication Number Publication Date
DE60201441D1 true DE60201441D1 (de) 2004-11-04
DE60201441T2 DE60201441T2 (de) 2005-10-27

Family

ID=7672767

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10104937A Expired - Fee Related DE10104937B4 (de) 2001-01-29 2001-01-29 Fluoreszenzpolarisation 2
DE60201441T Expired - Lifetime DE60201441T2 (de) 2001-01-29 2002-01-29 Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10104937A Expired - Fee Related DE10104937B4 (de) 2001-01-29 2001-01-29 Fluoreszenzpolarisation 2

Country Status (6)

Country Link
US (1) US20040121359A1 (de)
EP (1) EP1390529B1 (de)
AT (1) ATE278036T1 (de)
AU (1) AU2002238524A1 (de)
DE (2) DE10104937B4 (de)
WO (1) WO2002061123A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
DE10245779A1 (de) * 2002-10-01 2004-04-29 Epigenomics Ag Verfahren und Nukleinsäuren für die verbesserte Behandlung von proliferativen Erkrankungen von Brustzellen
US7371526B2 (en) * 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7368239B2 (en) * 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
JP2008515784A (ja) * 2004-09-21 2008-05-15 アプレラ コーポレイション メチル化ｄｎａ分析のための硫酸水素ナトリウムの改良代替物として硫黄求核剤を使用する方法
US7658288B2 (en) * 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
CN102618645B (zh) * 2012-03-30 2014-07-09 中国人民解放军第四军医大学 基于荧光偏振的egfr基因启动子甲基化状态均相检测法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8902689D0 (en) * 1989-02-07 1989-03-30 Ici Plc Assay method
US6059724A (en) * 1997-02-14 2000-05-09 Biosignal, Inc. System for predicting future health
JP3948503B2 (ja) * 1997-08-01 2007-07-25 誠 鶴岡 蛍光偏光法による核酸の測定方法およびＶｅｒｏ毒素生産菌の検出方法
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6180408B1 (en) * 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis

Also Published As

Publication number Publication date
EP1390529B1 (de) 2004-09-29
DE10104937B4 (de) 2005-03-17
EP1390529A2 (de) 2004-02-25
WO2002061123A3 (en) 2003-12-04
ATE278036T1 (de) 2004-10-15
AU2002238524A1 (en) 2002-08-12
DE60201441T2 (de) 2005-10-27
WO2002061123A2 (en) 2002-08-08
US20040121359A1 (en) 2004-06-24
DE10104937A1 (de) 2002-08-14

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