DE602007013396D1 - Transektion filamentöser pilze mit hohem durchsatz - Google Patents
Transektion filamentöser pilze mit hohem durchsatzInfo
- Publication number
- DE602007013396D1 DE602007013396D1 DE602007013396T DE602007013396T DE602007013396D1 DE 602007013396 D1 DE602007013396 D1 DE 602007013396D1 DE 602007013396 T DE602007013396 T DE 602007013396T DE 602007013396 T DE602007013396 T DE 602007013396T DE 602007013396 D1 DE602007013396 D1 DE 602007013396D1
- Authority
- DE
- Germany
- Prior art keywords
- transfection
- cells
- transeking
- high throughput
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Spinning Or Twisting Of Yarns (AREA)
- Mushroom Cultivation (AREA)
- Preliminary Treatment Of Fibers (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP06116095 | 2006-06-26 | ||
PCT/EP2007/056311 WO2008000715A1 (en) | 2006-06-26 | 2007-06-25 | High throughput transfection of filamentous fungi |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602007013396D1 true DE602007013396D1 (de) | 2011-05-05 |
Family
ID=38353831
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602007013396T Active DE602007013396D1 (de) | 2006-06-26 | 2007-06-25 | Transektion filamentöser pilze mit hohem durchsatz |
Country Status (6)
Country | Link |
---|---|
US (2) | US8697364B2 (de) |
EP (1) | EP2032703B1 (de) |
AT (1) | ATE503018T1 (de) |
DE (1) | DE602007013396D1 (de) |
DK (1) | DK2032703T3 (de) |
WO (1) | WO2008000715A1 (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140106398A1 (en) | 2011-03-11 | 2014-04-17 | Dsm Ip Assets B.V. | Vector-host system |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
KR20180084756A (ko) | 2015-12-07 | 2018-07-25 | 지머젠 인코포레이티드 | 코리네박테리움 글루타미컴으로부터의 프로모터 |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
JP2019519242A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | 細菌ヘモグロビンライブラリーを生成するための方法およびその使用 |
JP2019519241A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | グルコース透過酵素ライブラリーを生成するための方法およびその使用 |
JP2020503037A (ja) * | 2016-12-30 | 2020-01-30 | ザイマージェン インコーポレイテッド | 遺伝子操作および株精製のための自動化ステップを使用する真菌産生株を構築する方法 |
CN110719956A (zh) | 2017-06-06 | 2020-01-21 | 齐默尔根公司 | 用于改良真菌菌株的高通量基因组工程改造平台 |
US10913955B2 (en) | 2017-10-10 | 2021-02-09 | Dow Agrosciences Llc | Aldehyde dehydrogenase (ALDH1) nucleic acid molecules that control pathogens |
WO2019236848A1 (en) | 2018-06-06 | 2019-12-12 | Zymergen Inc. | Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production |
WO2021089452A1 (en) | 2019-11-04 | 2021-05-14 | Dsm Ip Assets B.V. | Low volume transfection |
US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU721471B2 (en) | 1995-08-03 | 2000-07-06 | Gist-Brocades B.V. | The use of homologous amdS genes as selectable markers |
AU4424597A (en) * | 1996-09-13 | 1998-04-02 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
AU2003258505A1 (en) * | 2002-07-05 | 2004-01-23 | Basf Aktiengesellschaft | Plasmid vectors for transformation of filamentous fungi |
JP2007534294A (ja) * | 2003-03-21 | 2007-11-29 | ジェネンコー・インターナショナル・インク | Cbh1相同体及び変異体cbh1セルラーゼ |
DE602004028724D1 (de) * | 2003-05-02 | 2010-09-30 | Novozymes Inc | Verfahren zur herstellung sezernierter polypeptide |
EP1673380A2 (de) | 2003-10-14 | 2006-06-28 | DSM IP Assets B.V. | Verfahren zur herstellung von modifizierten wirtszellen |
JP2005269920A (ja) * | 2004-03-23 | 2005-10-06 | Yamaguchi Technology Licensing Organization Ltd | 酵母の形質転換法 |
EP1766040B1 (de) * | 2004-05-27 | 2017-08-09 | Novozymes, Inc. | Verfahren für transformation und expressionsscreening von fadenpilzzellen mit einer dna-bibliothek |
WO2005118800A1 (en) * | 2004-05-27 | 2005-12-15 | Genencor International, Inc. | Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis |
US7943367B2 (en) * | 2006-09-20 | 2011-05-17 | Bio-Rad Laboratories, Inc. | High-throughput electroporation chamber with functional lid for risk reduction |
-
2007
- 2007-06-25 US US12/306,314 patent/US8697364B2/en active Active
- 2007-06-25 AT AT07786832T patent/ATE503018T1/de active
- 2007-06-25 DK DK07786832.1T patent/DK2032703T3/da active
- 2007-06-25 EP EP07786832A patent/EP2032703B1/de not_active Revoked
- 2007-06-25 WO PCT/EP2007/056311 patent/WO2008000715A1/en active Application Filing
- 2007-06-25 DE DE602007013396T patent/DE602007013396D1/de active Active
-
2014
- 2014-02-27 US US14/192,236 patent/US9334504B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP2032703B1 (de) | 2011-03-23 |
ATE503018T1 (de) | 2011-04-15 |
WO2008000715A1 (en) | 2008-01-03 |
US8697364B2 (en) | 2014-04-15 |
US20140178932A1 (en) | 2014-06-26 |
EP2032703A1 (de) | 2009-03-11 |
DK2032703T3 (da) | 2011-06-14 |
US20090280529A1 (en) | 2009-11-12 |
US9334504B2 (en) | 2016-05-10 |
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