DE60132208D1 - Regulation der genexpression unter verwendung von einkettigen, monomeren, liganden-abhängigen polypeptidschaltern - Google Patents

Regulation der genexpression unter verwendung von einkettigen, monomeren, liganden-abhängigen polypeptidschaltern

Info

Publication number
DE60132208D1
DE60132208D1 DE60132208T DE60132208T DE60132208D1 DE 60132208 D1 DE60132208 D1 DE 60132208D1 DE 60132208 T DE60132208 T DE 60132208T DE 60132208 T DE60132208 T DE 60132208T DE 60132208 D1 DE60132208 D1 DE 60132208D1
Authority
DE
Germany
Prior art keywords
chain
ligand
monomer
regulation
switches
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60132208T
Other languages
English (en)
Other versions
DE60132208T2 (de
Inventor
Roger Beerli
Ulrich Schopfer
Carlos F Barbas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Scripps Research Institute
Original Assignee
Novartis AG
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG, Scripps Research Institute filed Critical Novartis AG
Publication of DE60132208D1 publication Critical patent/DE60132208D1/de
Application granted granted Critical
Publication of DE60132208T2 publication Critical patent/DE60132208T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702Regulators; Modulating activity
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C07K2319/715Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16 containing a domain for ligand dependent transcriptional activation, e.g. containing a steroid receptor domain
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60132208T 2000-07-18 2001-07-16 Regulation der genexpression unter verwendung von einkettigen, monomeren, liganden-abhängigen polypeptidschaltern Expired - Lifetime DE60132208T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US619063 1996-03-20
US61906300A 2000-07-18 2000-07-18
PCT/EP2001/008190 WO2002006463A2 (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches

Publications (2)

Publication Number Publication Date
DE60132208D1 true DE60132208D1 (de) 2008-02-14
DE60132208T2 DE60132208T2 (de) 2008-12-24

Family

ID=24480304

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60132208T Expired - Lifetime DE60132208T2 (de) 2000-07-18 2001-07-16 Regulation der genexpression unter verwendung von einkettigen, monomeren, liganden-abhängigen polypeptidschaltern

Country Status (10)

Country Link
EP (2) EP1908835A3 (de)
JP (1) JP2004504029A (de)
AT (1) ATE382691T1 (de)
AU (2) AU2001272544B2 (de)
CA (1) CA2415726A1 (de)
DE (1) DE60132208T2 (de)
ES (1) ES2299494T3 (de)
IL (1) IL153833A0 (de)
NZ (1) NZ523649A (de)
WO (1) WO2002006463A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329728B1 (en) 1999-10-25 2008-02-12 The Scripps Research Institute Ligand activated transcriptional regulator proteins
US7011972B2 (en) 2000-07-18 2006-03-14 The Scripps Research Institute Fusion polypeptide comprising two ligand binding domains
NZ527937A (en) 2001-03-13 2005-03-24 Novartis Ag Stable cell lines to express high levels of lentiviral Gag/Pol proteins for generation of HIV- or BIV- based lentiviral vectors
ATE403676T1 (de) 2001-05-31 2008-08-15 Novartis Ag Neue östrogenrezeptorligandenbindungsdomänen varianten und neue liganden und pharmazeutische zusammensetzungen
US7927840B2 (en) 2006-09-11 2011-04-19 Gen Probe Incorporated Method for detecting West Nile Virus nucleic acids in the 3′ non-coding region
EP2366803B8 (de) 2002-10-16 2015-08-19 Gen-Probe Incorporated Zusammensetzungen und Verfahren zum Nachweis des West Nil Viruses

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981784A (en) * 1987-12-02 1991-01-01 The Salk Institute For Biological Studies Retinoic acid receptor method
EP0935657B1 (de) * 1996-10-29 2007-08-29 Baylor College Of Medicine Modifizierte steroid-hormon rezeptoren
JP2003524368A (ja) * 1997-08-26 2003-08-19 アリアド ジーン セラピューティクス インコーポレイテッド ニ量化ドメイン、三量化ドメインまたは四量化ドメインおよび補足的非相同転写活性化ドメイン、転写抑制ドメイン、dna結合ドメインまたはリガンド結合ドメインを含む融合蛋白
CA2323064C (en) * 1998-03-17 2011-05-31 Gendaq Limited Nucleic acid binding proteins
US6140081A (en) * 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US7057015B1 (en) * 1999-10-20 2006-06-06 The Salk Institute For Biological Studies Hormone receptor functional dimers and methods of their use
US7329728B1 (en) * 1999-10-25 2008-02-12 The Scripps Research Institute Ligand activated transcriptional regulator proteins

Also Published As

Publication number Publication date
EP1908835A2 (de) 2008-04-09
EP1303606B1 (de) 2008-01-02
IL153833A0 (en) 2003-07-31
ES2299494T3 (es) 2008-06-01
AU7254401A (en) 2002-01-30
AU2001272544B2 (en) 2005-12-01
NZ523649A (en) 2005-02-25
EP1908835A3 (de) 2008-06-18
DE60132208T2 (de) 2008-12-24
JP2004504029A (ja) 2004-02-12
WO2002006463A3 (en) 2002-07-25
EP1303606A2 (de) 2003-04-23
ATE382691T1 (de) 2008-01-15
WO2002006463A2 (en) 2002-01-24
CA2415726A1 (en) 2002-01-24

Similar Documents

Publication Publication Date Title
DE60025037D1 (de) "functional genomics" unter verwendung von zink finger proteinen
ATE320500T1 (de) Nukleotid-sequenzen zur kontrolle der exprimierung von dns-sequenzen in einem zellulärem wirt
ATE529509T1 (de) Selektion von proteinen unter verwendung von rna- protein-fusionen
DE69534629D1 (de) Derivate von zinkfingerproteinen und methoden
ATE309347T1 (de) Protocadherin-proteine und verwendungen davon
ATE309536T1 (de) Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
ATE516354T1 (de) Apo-2-rezeptor
DE69638183D1 (de) Verwendung von nukleinsäureliganden in der durchflusszytometrie
PT920498E (pt) Adn regulador transcricional de ef-1x de hamster
DE50012863D1 (de) Strukturprotein von adeno-assoziiertem virus mit veränderten chromatographischen eigenschaften
DK0723588T3 (da) Activinreceptorlignende kinase (ALK) hörende til TGF-receptorfamilien og/eller BMP-receptorfamilien
DE69839444D1 (de) Smad 6 und dessen anwendungen
DK0960192T3 (da) Syntetiske polynukleotider
DE60132208D1 (de) Regulation der genexpression unter verwendung von einkettigen, monomeren, liganden-abhängigen polypeptidschaltern
DE60128830D1 (de) Regulierung der expression von eer-7, ein mitglied der lysyl oxidase genfamilie, durch oestrogenrezeptoren
DE69928395D1 (de) Cyclin-e2 proteine und dafür kodierende gene
DK1009817T3 (da) RTD-receptor
WO2000008181A3 (en) Chp polypeptide, a ligand of pak65
ATE274351T1 (de) Verwendung von isolierten domänen des type-iv- kollagens zur änderung der zell- und gewebewechselwirkung
TR200003695T2 (tr) Anjiyostatin bağlayıcı protein.
DE69809930D1 (de) Isolierung und verwendung von nukleinsäuremolekülen, welche für mitglieder der ssx-familie kodieren
DE69806107T2 (de) Methoden und reagenzien zur regulierung von apoptosis
ATE324446T1 (de) Mutante formen von etxb und ctxb und ihre verwendung als träger
WO2003044166A3 (en) METHODS AND COMPOSITIONS FOR REGULATION OF GENE EXPRESSION THROUGH MODULATION OF mRNA TURNOVER
DK1007649T3 (da) Fremgangsmåder til screening for interaktioner mellem transkriptionsfaktor og coaktivator

Legal Events

Date Code Title Description
8364 No opposition during term of opposition