DE60017875D1 - Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen - Google Patents

Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen

Info

Publication number
DE60017875D1
DE60017875D1 DE60017875T DE60017875T DE60017875D1 DE 60017875 D1 DE60017875 D1 DE 60017875D1 DE 60017875 T DE60017875 T DE 60017875T DE 60017875 T DE60017875 T DE 60017875T DE 60017875 D1 DE60017875 D1 DE 60017875D1
Authority
DE
Germany
Prior art keywords
neural stem
culture
cell culture
proliferation
stem cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60017875T
Other languages
English (en)
Other versions
DE60017875T2 (de
Inventor
Nobuko Uchida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
StemCells California Inc
Original Assignee
StemCells Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by StemCells Inc filed Critical StemCells Inc
Publication of DE60017875D1 publication Critical patent/DE60017875D1/de
Application granted granted Critical
Publication of DE60017875T2 publication Critical patent/DE60017875T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Neurosurgery (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60017875T 1999-02-26 2000-02-25 Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen Expired - Lifetime DE60017875T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US258529 1999-02-26
US09/258,529 US6238922B1 (en) 1999-02-26 1999-02-26 Use of collagenase in the preparation of neural stem cell cultures
PCT/US2000/005402 WO2000050572A1 (en) 1999-02-26 2000-02-25 Use of collagenase in the preparation of neural stem cell cultures

Publications (2)

Publication Number Publication Date
DE60017875D1 true DE60017875D1 (de) 2005-03-10
DE60017875T2 DE60017875T2 (de) 2006-04-06

Family

ID=22980962

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60017875T Expired - Lifetime DE60017875T2 (de) 1999-02-26 2000-02-25 Verwendung von collagenase in der zubereitung von neuralen stammzellkulturen

Country Status (10)

Country Link
US (3) US6238922B1 (de)
EP (2) EP1533367A1 (de)
JP (2) JP4585124B2 (de)
AT (1) ATE288481T1 (de)
AU (1) AU764499B2 (de)
CA (1) CA2363851C (de)
DE (1) DE60017875T2 (de)
DK (1) DK1155116T3 (de)
ES (1) ES2235847T3 (de)
WO (1) WO2000050572A1 (de)

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US6238922B1 (en) * 1999-02-26 2001-05-29 Stemcells, Inc. Use of collagenase in the preparation of neural stem cell cultures
US20050042749A1 (en) * 2001-05-16 2005-02-24 Carpenter Melissa K. Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease
GB2379447B (en) * 2000-05-17 2004-12-29 Geron Corp Neural progenitor cell populations
US7250294B2 (en) * 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
US7838292B1 (en) * 2001-03-29 2010-11-23 University Of Louisville Research Foundation, Inc. Methods for obtaining adult human olfactory progenitor cells
US20050101014A1 (en) * 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
JP2006506089A (ja) * 2002-11-18 2006-02-23 エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ 細胞および／または核酸分子単離のための方法およびシステム
CA2527847C (en) * 2003-06-12 2015-09-29 Yeda Research & Development Co. Ltd Enhancement of oligodendrocyte differentiation
ATE493504T1 (de) * 2003-09-12 2011-01-15 Stemcell Technologies Inc Test für neurale koloniebildung
US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
ES2579804T3 (es) * 2003-12-02 2016-08-16 Celavie Biosciences, Llc Composiciones y métodos para la propagación de células progenitoras neurales
WO2005079250A2 (en) * 2004-02-13 2005-09-01 Cornell Research Foundation, Inc. Purines are self-renewal signals for neural stem cells, and purine receptor antagonists promote neuronal and glial differentiation therefrom
KR100563297B1 (ko) * 2004-06-16 2006-03-27 시 명 김 골프공 이송장치
US7456019B2 (en) 2004-06-30 2008-11-25 Regenetech, Inc. Three-dimensional cell to tissue development process
US20060228795A1 (en) * 2004-06-30 2006-10-12 Parker Clayton R Apparatus for enhancing proliferation of cells in a small-scale cell culturing container
US20080044890A1 (en) * 2004-06-30 2008-02-21 Parker Clayton R Interchangable sleeve for enhancing proliferation of cells in a rotating bioreactor
NZ565683A (en) * 2005-07-08 2011-04-29 Braincells Inc Methods for identifying agents and conditions that modulate neurogenesis in human neural cells
US20070059778A1 (en) * 2005-09-12 2007-03-15 Wognum Albertus W Modified colony assay
WO2007130060A2 (en) 2006-05-03 2007-11-15 Schepens Eye Research Isolation and therapeutic application of adult retinal stem cells collected from extra-retinal tissues
EP2484308A1 (de) * 2006-07-24 2012-08-08 International Stem Cell Corporation Synthetische Hornhaut aus Netzhautstammzellen
EP1900810A1 (de) * 2006-09-15 2008-03-19 Celogos Verfahren zur Zellextraktion und Zellselektion
US20100196328A1 (en) * 2007-04-25 2010-08-05 Tonya Bliss ISCHEMIA-INDUCED NEOVASCULARIZATION IS ENHANCED BY hCNS-SC TRANSPLANTATION
CN101939362A (zh) 2008-01-30 2011-01-05 杰龙公司 用于培养干细胞衍生的少突胶质细胞前体细胞的合成表面
US8093053B2 (en) * 2008-03-17 2012-01-10 Uti Limited Partnership Methods and compositions for culturing of neural precursor cells
WO2010018996A2 (ko) * 2008-08-12 2010-02-18 연세대학교 산학협력단 인간 신경줄기세포 및 이를 이용한 중추 또는 말초 신경계 질환 및 손상 치료용 약학적 조성물
US8309343B2 (en) 2008-12-01 2012-11-13 Baxter International Inc. Apparatus and method for processing biological material
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
EP2741756A4 (de) * 2011-08-09 2015-04-22 Lpath Inc Stammzellentherapie unter verwendung von lysophosphatidsäure
NZ741539A (en) 2015-11-06 2024-03-22 Ventana Med Syst Inc Representative diagnostics
CN109022360A (zh) * 2018-07-03 2018-12-18 湖南未名三胞转化医学科技有限公司 自体脂肪干细胞共培养促进外周血造血干细胞增殖方法

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US5411883A (en) 1989-12-26 1995-05-02 Somatix Therapy Corporation Proliferated neuron progenitor cell product and process
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
US5750376A (en) * 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
ATE240389T1 (de) * 1991-07-08 2003-05-15 Neurospheres Holdings Ltd Auf wachstumsfaktoren reagierende neurale vorläuferzellen, die in vitro vermehrt werden können.
WO1994002593A1 (en) * 1992-07-27 1994-02-03 California Institute Of Technology Mammalian multipotent neural stem cells
EP0664832B1 (de) * 1992-10-16 2002-07-24 Neurospheres Holdings Ltd. Remyelination unter verwendung von neuronalen stammzellen
AU3338595A (en) 1994-07-29 1996-03-04 Dalhousie University Propagation and inducible differentiation of human fetal central nervous system progenitor cells
KR970707272A (ko) 1994-11-14 1997-12-01 스코트 디. 코르맥 신경 간세포 증식 조절 방법(regulation of neural stem cell proliferation)
CN1217746A (zh) * 1996-04-29 1999-05-26 勒芬研究与发展公司 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用
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US6238922B1 (en) * 1999-02-26 2001-05-29 Stemcells, Inc. Use of collagenase in the preparation of neural stem cell cultures

Also Published As

Publication number Publication date
EP1155116A1 (de) 2001-11-21
CA2363851A1 (en) 2000-08-31
JP4585124B2 (ja) 2010-11-24
EP1155116B1 (de) 2005-02-02
WO2000050572A1 (en) 2000-08-31
ES2235847T3 (es) 2005-07-16
US20010055808A1 (en) 2001-12-27
DK1155116T3 (da) 2005-05-30
US7049141B1 (en) 2006-05-23
ATE288481T1 (de) 2005-02-15
JP2002536991A (ja) 2002-11-05
DE60017875T2 (de) 2006-04-06
WO2000050572A9 (en) 2001-08-30
EP1533367A1 (de) 2005-05-25
US6238922B1 (en) 2001-05-29
JP2010227127A (ja) 2010-10-14
AU764499B2 (en) 2003-08-21
AU3716300A (en) 2000-09-14
CA2363851C (en) 2011-01-25

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Legal Events

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Owner name: STEMCELLS CALIFORNIA, INC., PALO ALTO, CALIF., US