DE4216696C2 - Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays - Google Patents

Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays

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Publication number
DE4216696C2
DE4216696C2 DE4216696A DE4216696A DE4216696C2 DE 4216696 C2 DE4216696 C2 DE 4216696C2 DE 4216696 A DE4216696 A DE 4216696A DE 4216696 A DE4216696 A DE 4216696A DE 4216696 C2 DE4216696 C2 DE 4216696C2
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DE
Germany
Prior art keywords
molecules
analyte
zone
molecule
measurement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE4216696A
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German (de)
English (en)
Other versions
DE4216696A1 (de
Inventor
Karl Prof Dr Cammann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CAMMANN, KARL, PROF. DR., 48155 MUENSTER, DE
Original Assignee
Deutsche Aerospace AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsche Aerospace AG filed Critical Deutsche Aerospace AG
Priority to DE4216696A priority Critical patent/DE4216696C2/de
Publication of DE4216696A1 publication Critical patent/DE4216696A1/de
Application granted granted Critical
Publication of DE4216696C2 publication Critical patent/DE4216696C2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE4216696A 1992-04-10 1992-05-20 Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays Expired - Fee Related DE4216696C2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE4216696A DE4216696C2 (de) 1992-04-10 1992-05-20 Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4212148 1992-04-10
DE4216696A DE4216696C2 (de) 1992-04-10 1992-05-20 Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays

Publications (2)

Publication Number Publication Date
DE4216696A1 DE4216696A1 (de) 1993-10-28
DE4216696C2 true DE4216696C2 (de) 1995-01-26

Family

ID=6456593

Family Applications (1)

Application Number Title Priority Date Filing Date
DE4216696A Expired - Fee Related DE4216696C2 (de) 1992-04-10 1992-05-20 Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays

Country Status (2)

Country Link
DE (1) DE4216696C2 (fr)
WO (1) WO1993021530A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19621165C1 (de) * 1996-05-24 1997-10-02 Karlsruhe Forschzent Verfahren zur Herstellung einer Probe aus immobilisierten Makromolekülen
DE19622458A1 (de) * 1996-05-24 1997-11-27 Senslab Ges Zur Entwicklung Un Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay
DE19741716A1 (de) * 1997-09-22 1999-03-25 Hoechst Ag Adressierbares modulares Erkennungssystem, seine Herstellung und Verwendung
DE19742227A1 (de) * 1997-09-25 1999-04-01 Juergen Prof Dipl Phys Wolfrum Verfahren zum Sequenzieren eines einzelnen DNA-Moleküls
DE10106654A1 (de) * 2001-02-12 2002-09-05 November Ag Molekulare Medizin Verfahren zum Nachweis und/oder zur Quantifizierung eines Analyten

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19620636A1 (de) * 1995-06-01 1996-12-19 Fraunhofer Ges Forschung Vorrichtung zur Durchführung chemischer oder biochemischer Affinitätsverfahren und Verfahren zum Betreiben der Vorrichtung
GB9606850D0 (en) * 1996-04-01 1996-06-05 Univ Liverpool An assay system and novel labelled compounds for use therwith
CA2309599C (fr) * 1997-11-21 2009-03-17 Unilever Plc Ameliorations apportees aux analyses de deplacement
DE19964220C2 (de) 1998-11-23 2003-07-03 Friz Biochem Gmbh Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche
CA2371938A1 (fr) * 1999-01-18 2000-07-20 Gerhard Hartwich Procede de detection electrochimique d'hybridation d'acide nucleique-oligomere
DE10141691A1 (de) * 2001-08-25 2003-03-13 Friz Biochem Gmbh Verdrängungsassay zur Detektion von Ligat-Ligand-Assoziationsereignissen
WO2003018834A2 (fr) 2001-08-25 2003-03-06 Friz Biochem Gmbh Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique
US7341834B2 (en) 2003-12-15 2008-03-11 Geneohn Sciences, Inc. Multiplexed electrochemical detection system and method
CN1296700C (zh) * 2003-12-31 2007-01-24 中国地质大学（武汉） 矿物材料红外荧光分析法
WO2006065598A2 (fr) * 2004-12-13 2006-06-22 Geneohm Sciences, Inc. Cartouches fluidiques utilisees dans la detection electrochimique de l'adn

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4277560A (en) * 1978-10-24 1981-07-07 Technicon Instruments Corporation Enzyme immunoassays using immobilized reagents in a flowing stream
US4366241A (en) * 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
US4582809A (en) * 1982-06-14 1986-04-15 Myron J. Block Apparatus including optical fiber for fluorescence immunoassay
DE3577748D1 (de) * 1984-06-13 1990-06-21 Unilever Nv Vorrichtungen zur verwendung in chemischen analyseverfahren.
US4859583A (en) * 1985-02-25 1989-08-22 Amoco Corporation Chemiluminescent immunochemical technique for low molecular weight antigens
US4997526A (en) * 1985-03-19 1991-03-05 Eic Laboratories, Inc. Assaying for a biologically active component
US4746631A (en) * 1985-05-09 1988-05-24 Ultra Diagnostics Corporation Immunoassay method, device, and test kit
DE3916432A1 (de) * 1989-05-20 1990-11-22 Cammann Karl Verfahren und vorrichtung zur konzentrationsbestimmung eines antikoerper-antigenpaares
US5183740A (en) * 1990-02-23 1993-02-02 The United States Of America As Represented By The Secretary Of The Navy Flow immunosensor method and apparatus

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19621165C1 (de) * 1996-05-24 1997-10-02 Karlsruhe Forschzent Verfahren zur Herstellung einer Probe aus immobilisierten Makromolekülen
DE19622458A1 (de) * 1996-05-24 1997-11-27 Senslab Ges Zur Entwicklung Un Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay
DE19622458C2 (de) * 1996-05-24 1998-03-26 Senslab Ges Zur Entwicklung Un Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay
DE19741716A1 (de) * 1997-09-22 1999-03-25 Hoechst Ag Adressierbares modulares Erkennungssystem, seine Herstellung und Verwendung
DE19742227A1 (de) * 1997-09-25 1999-04-01 Juergen Prof Dipl Phys Wolfrum Verfahren zum Sequenzieren eines einzelnen DNA-Moleküls
DE10106654A1 (de) * 2001-02-12 2002-09-05 November Ag Molekulare Medizin Verfahren zum Nachweis und/oder zur Quantifizierung eines Analyten

Also Published As

Publication number Publication date
DE4216696A1 (de) 1993-10-28
WO1993021530A1 (fr) 1993-10-28

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8327 Change in the person/name/address of the patent owner

Owner name: DAIMLER-BENZ AEROSPACE AKTIENGESELLSCHAFT, 80804 M

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: DAIMLERCHRYSLER AEROSPACE AKTIENGESELLSCHAFT, 8099

8327 Change in the person/name/address of the patent owner

Owner name: CAMMANN, KARL, PROF. DR., 48155 MUENSTER, DE

8339 Ceased/non-payment of the annual fee