DE4013593A1 - Verfahren und sensor fuer amperometrische messprinzipien mit dickschicht-biosensoren - Google Patents
Verfahren und sensor fuer amperometrische messprinzipien mit dickschicht-biosensorenInfo
- Publication number
- DE4013593A1 DE4013593A1 DE19904013593 DE4013593A DE4013593A1 DE 4013593 A1 DE4013593 A1 DE 4013593A1 DE 19904013593 DE19904013593 DE 19904013593 DE 4013593 A DE4013593 A DE 4013593A DE 4013593 A1 DE4013593 A1 DE 4013593A1
- Authority
- DE
- Germany
- Prior art keywords
- thick
- electrodes
- amperometric
- film
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005259 measurement Methods 0.000 title claims abstract description 11
- 239000004020 conductor Substances 0.000 title abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 35
- 239000000758 substrate Substances 0.000 claims abstract description 25
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 17
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 9
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 9
- 108020005199 Dehydrogenases Proteins 0.000 claims abstract description 6
- 229910000510 noble metal Inorganic materials 0.000 claims abstract 2
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 229940088598 enzyme Drugs 0.000 claims description 17
- 239000000919 ceramic Substances 0.000 claims description 9
- 238000005516 engineering process Methods 0.000 claims description 9
- 239000010970 precious metal Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 108010015776 Glucose oxidase Proteins 0.000 claims description 4
- 239000004366 Glucose oxidase Substances 0.000 claims description 3
- 150000001721 carbon Chemical class 0.000 claims description 3
- 229940116332 glucose oxidase Drugs 0.000 claims description 3
- 235000019420 glucose oxidase Nutrition 0.000 claims description 3
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 claims description 2
- 101710088194 Dehydrogenase Proteins 0.000 claims description 2
- 239000005662 Paraffin oil Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 2
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- PCCVSPMFGIFTHU-UHFFFAOYSA-N tetracyanoquinodimethane Chemical group N#CC(C#N)=C1C=CC(=C(C#N)C#N)C=C1 PCCVSPMFGIFTHU-UHFFFAOYSA-N 0.000 claims description 2
- KBZALDXXIMXRBJ-UHFFFAOYSA-N 5-methylphenazin-5-ium Chemical compound C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 KBZALDXXIMXRBJ-UHFFFAOYSA-N 0.000 claims 1
- 229910010293 ceramic material Inorganic materials 0.000 claims 1
- 239000007772 electrode material Substances 0.000 abstract description 4
- 239000000306 component Substances 0.000 description 10
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 239000010931 gold Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- FHCPAXDKURNIOZ-UHFFFAOYSA-N tetrathiafulvalene Chemical compound S1C=CSC1=C1SC=CS1 FHCPAXDKURNIOZ-UHFFFAOYSA-N 0.000 description 4
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 3
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 229910021607 Silver chloride Inorganic materials 0.000 description 3
- 239000003245 coal Substances 0.000 description 3
- 238000003411 electrode reaction Methods 0.000 description 3
- KYMNSBSWJPFUJH-UHFFFAOYSA-N iron;5-methylcyclopenta-1,3-diene;methylcyclopentane Chemical compound [Fe].C[C-]1C=CC=C1.C[C-]1[CH-][CH-][CH-][CH-]1 KYMNSBSWJPFUJH-UHFFFAOYSA-N 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
- 238000004082 amperometric method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- MNIQECRMTVGZBM-UHFFFAOYSA-N 3-(1-methylpyrrolidin-2-yl)pyridine;7h-purin-6-amine Chemical compound NC1=NC=NC2=C1NC=N2.CN1CCCC1C1=CC=CN=C1 MNIQECRMTVGZBM-UHFFFAOYSA-N 0.000 description 1
- 239000002262 Schiff base Substances 0.000 description 1
- 150000004753 Schiff bases Chemical class 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000010292 electrical insulation Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- CATMPQFFVNKDEY-AAEUAGOBSA-N gamma-Glu-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)CC[C@H](N)C(O)=O)C(O)=O)=CNC2=C1 CATMPQFFVNKDEY-AAEUAGOBSA-N 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 238000004313 potentiometry Methods 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19904013593 DE4013593A1 (de) | 1990-04-27 | 1990-04-27 | Verfahren und sensor fuer amperometrische messprinzipien mit dickschicht-biosensoren |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19904013593 DE4013593A1 (de) | 1990-04-27 | 1990-04-27 | Verfahren und sensor fuer amperometrische messprinzipien mit dickschicht-biosensoren |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE4013593A1 true DE4013593A1 (de) | 1991-10-31 |
| DE4013593C2 DE4013593C2 (enExample) | 1993-06-24 |
Family
ID=6405291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19904013593 Granted DE4013593A1 (de) | 1990-04-27 | 1990-04-27 | Verfahren und sensor fuer amperometrische messprinzipien mit dickschicht-biosensoren |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE4013593A1 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5770439A (en) * | 1991-09-28 | 1998-06-23 | Gesellschaft Fur Biotechnologische Forschung Mbb (Gbf) | Thick-film conductivity enzyme electrodes in a vertical stack as biosensor |
| DE19930104C1 (de) * | 1999-07-01 | 2001-02-15 | Fraunhofer Ges Forschung | Verfahren zur Herstellung einer Elektrodenanordnung |
| WO2004029605A1 (en) * | 2002-09-30 | 2004-04-08 | Infopia Co., Ltd. | Apparatus and method for measuring reaction result of samples on biosensor |
| EP1199751A3 (de) * | 1998-06-30 | 2005-12-07 | Micronas GmbH | Chip-Anordnung |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19621227C2 (de) * | 1996-05-25 | 2003-02-20 | Micronas Gmbh | Sensorelement zum Nachweis von flüssigen, gasförmigen oder fluiden Substanzen |
| DE20017268U1 (de) | 2000-10-07 | 2001-03-29 | DECHEMA Gesellschaft für Chemische Technik und Biotechnologie eV, 60486 Frankfurt | Elektrochemische Messzelle zur Bestimmung der Zellzahl und Aktivität von biologischen Systemen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4655880A (en) * | 1983-08-01 | 1987-04-07 | Case Western Reserve University | Apparatus and method for sensing species, substances and substrates using oxidase |
| DE3725597C2 (enExample) * | 1987-08-01 | 1989-05-03 | Siegert Gmbh, 8501 Cadolzburg, De |
-
1990
- 1990-04-27 DE DE19904013593 patent/DE4013593A1/de active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4655880A (en) * | 1983-08-01 | 1987-04-07 | Case Western Reserve University | Apparatus and method for sensing species, substances and substrates using oxidase |
| DE3725597C2 (enExample) * | 1987-08-01 | 1989-05-03 | Siegert Gmbh, 8501 Cadolzburg, De |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5770439A (en) * | 1991-09-28 | 1998-06-23 | Gesellschaft Fur Biotechnologische Forschung Mbb (Gbf) | Thick-film conductivity enzyme electrodes in a vertical stack as biosensor |
| EP1199751A3 (de) * | 1998-06-30 | 2005-12-07 | Micronas GmbH | Chip-Anordnung |
| DE19930104C1 (de) * | 1999-07-01 | 2001-02-15 | Fraunhofer Ges Forschung | Verfahren zur Herstellung einer Elektrodenanordnung |
| WO2004029605A1 (en) * | 2002-09-30 | 2004-04-08 | Infopia Co., Ltd. | Apparatus and method for measuring reaction result of samples on biosensor |
| CN1302281C (zh) * | 2002-09-30 | 2007-02-28 | 因福皮亚有限公司 | 用于测定生物传感器上样品的反应结果的设备和方法 |
| US7678261B2 (en) | 2002-09-30 | 2010-03-16 | Infopia Co., Ltd. | Apparatus and method for measuring reaction result of samples on biosensor |
Also Published As
| Publication number | Publication date |
|---|---|
| DE4013593C2 (enExample) | 1993-06-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |