DE3851259T2 - Mutierter t-PA mit Kringle-Ersatz. - Google Patents

Mutierter t-PA mit Kringle-Ersatz.

Info

Publication number
DE3851259T2
DE3851259T2 DE3851259T DE3851259T DE3851259T2 DE 3851259 T2 DE3851259 T2 DE 3851259T2 DE 3851259 T DE3851259 T DE 3851259T DE 3851259 T DE3851259 T DE 3851259T DE 3851259 T2 DE3851259 T2 DE 3851259T2
Authority
DE
Germany
Prior art keywords
analogs
kringle
fibrin
native
disclosed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3851259T
Other languages
English (en)
Other versions
DE3851259D1 (de
Inventor
Eileen R Mulvihill
Bjorn A Nexo
Shinji Yoshitake
Yasunori Ikeda
Suguru Suzuki
Akira Hashimoto
Teruaki Yuzuriha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Eisai R&D Management Co Ltd
Zymogenetics Inc
Original Assignee
Novo Industri AS
Eisai Co Ltd
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Industri AS, Eisai Co Ltd, Zymogenetics Inc filed Critical Novo Industri AS
Application granted granted Critical
Publication of DE3851259D1 publication Critical patent/DE3851259D1/de
Publication of DE3851259T2 publication Critical patent/DE3851259T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
DE3851259T 1987-06-04 1988-06-03 Mutierter t-PA mit Kringle-Ersatz. Expired - Lifetime DE3851259T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5821787A 1987-06-04 1987-06-04

Publications (2)

Publication Number Publication Date
DE3851259D1 DE3851259D1 (de) 1994-10-06
DE3851259T2 true DE3851259T2 (de) 1994-12-15

Family

ID=22015426

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3851259T Expired - Lifetime DE3851259T2 (de) 1987-06-04 1988-06-03 Mutierter t-PA mit Kringle-Ersatz.

Country Status (14)

Country Link
EP (1) EP0293934B1 (de)
JP (1) JPS6485078A (de)
KR (1) KR970005251B1 (de)
AT (1) ATE110772T1 (de)
AU (1) AU617323B2 (de)
CA (1) CA1341447C (de)
DE (1) DE3851259T2 (de)
DK (1) DK302288A (de)
ES (1) ES2058180T3 (de)
FI (1) FI882628A (de)
IE (1) IE64782B1 (de)
NO (1) NO179754C (de)
NZ (1) NZ224915A (de)
ZA (1) ZA883958B (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187098A (en) * 1984-03-02 1993-02-16 Board Of Regents Of The University Of Oklahoma DNA encoding hybrid streptokinases with plasminogen fibrin binding domains
US5714372A (en) * 1985-04-22 1998-02-03 Genentech, Inc. Tissue plasminogen activator variants
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
DE3836200A1 (de) * 1988-10-24 1990-04-26 Boehringer Mannheim Gmbh Domaenenduplikations-muteine des gewebsplasminogen-aktivators (t-pa)
GB8901422D0 (en) * 1989-01-23 1989-03-15 Fujisawa Pharmaceutical Co New tissue plasminogen activator
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
GB9019120D0 (en) * 1990-09-01 1990-10-17 Beecham Group Plc Novel compounds
US5272076A (en) * 1991-06-13 1993-12-21 Eli Lilly And Company Compounds and methods for treatment of thromboembolic disorders using an adduct of t-PA
JP3112480B2 (ja) * 1991-12-16 2000-11-27 ジェネンテク，インコーポレイテッド 新規な組織プラスミノーゲン活性化因子変異体
DE10199044I2 (de) * 1992-06-03 2006-02-09 Genentech Inc Varianten des Gewebeplasminogenaktivators mit verbesserter Therapeutischer wirkung
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0125254A1 (de) * 1982-10-28 1984-11-21 Beecham Group Plc Enzymderivate und deren verwendung bei der behandlung von trombosen
DK149786A (da) * 1985-04-04 1986-10-04 Beecham Group Plc Proteolytisk enzym
FR2581652B1 (fr) * 1985-04-22 1989-12-22 Genentech Inc Nouveaux mutants de l'activateur tissulaire du plasminogene humain
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
DE3643158A1 (de) * 1986-04-21 1987-11-19 Boehringer Mannheim Gmbh Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung
ES2069536T3 (es) * 1987-06-04 1995-05-16 Zymogenetics Inc Analogos del activador del plasminogeno tisular que tienen dominios modificados del factor de crecimiento.

Also Published As

Publication number Publication date
AU617323B2 (en) 1991-11-28
IE881681L (en) 1988-12-04
IE64782B1 (en) 1995-09-06
DK302288A (da) 1989-02-03
NO882453L (no) 1988-12-05
FI882628A (fi) 1988-12-05
ES2058180T3 (es) 1994-11-01
JPS6485078A (en) 1989-03-30
AU1741088A (en) 1988-12-08
JPH0448433B2 (de) 1992-08-06
KR970005251B1 (ko) 1997-04-14
ATE110772T1 (de) 1994-09-15
KR890000665A (ko) 1989-03-16
NO179754B (no) 1996-09-02
EP0293934A1 (de) 1988-12-07
NO179754C (no) 1996-12-11
DK302288D0 (da) 1988-06-03
FI882628A0 (fi) 1988-06-03
NZ224915A (en) 1989-12-21
DE3851259D1 (de) 1994-10-06
EP0293934B1 (de) 1994-08-31
CA1341447C (en) 2003-11-18
NO882453D0 (no) 1988-06-03
ZA883958B (en) 1989-07-26

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DE3686410D1 (de) Stabilisierte zusammensetzungen von menschlichen aktivatoren des gewebeplasminogens.
DE3853100T2 (de) Gewebs-Plasminogen-Aktivator-Analoge mit modifizierten Wachstumsfaktordomänen.
ES2088860T3 (es) Activador plasminogenico de tipo tisular humano modificado y su preparacion.
ATE103178T1 (de) T-pa pro stabilisierung.
ZA884012B (en) Tissue plasminogen activator analogs having modified growth factor domains
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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: EISAI R&D MANAGEMENT CO., LTD., TOKYO, JP

Owner name: NOVO INDUSTRI A/S, BAGSVAERD, DK

Owner name: ZYMOGENETICS, INC., SEATTLE, WASH., US