DE3682166D1 - Herstellung von insektizidprotein von bacillus thuringiensis subsp. aizawai ipl durch die expression von insektizidprotein-gen in wirtszellen. - Google Patents

Herstellung von insektizidprotein von bacillus thuringiensis subsp. aizawai ipl durch die expression von insektizidprotein-gen in wirtszellen.

Info

Publication number
DE3682166D1
DE3682166D1 DE8686308180T DE3682166T DE3682166D1 DE 3682166 D1 DE3682166 D1 DE 3682166D1 DE 8686308180 T DE8686308180 T DE 8686308180T DE 3682166 T DE3682166 T DE 3682166T DE 3682166 D1 DE3682166 D1 DE 3682166D1
Authority
DE
Germany
Prior art keywords
insecticide protein
expressing
production
host cells
bacillus thuringiensis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686308180T
Other languages
English (en)
Inventor
Kenji Oeda
Kazuyuki Oshie
Masatoshi Shimizu
Keiko Nakamura
Hideo Ohkawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Chemical Co Ltd
Original Assignee
Sumitomo Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP60242528A external-priority patent/JPS62100292A/ja
Priority claimed from JP61024563A external-priority patent/JPS62181784A/ja
Priority claimed from JP61040925A external-priority patent/JP2779440B2/ja
Application filed by Sumitomo Chemical Co Ltd filed Critical Sumitomo Chemical Co Ltd
Application granted granted Critical
Publication of DE3682166D1 publication Critical patent/DE3682166D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
DE8686308180T 1985-10-28 1986-10-21 Herstellung von insektizidprotein von bacillus thuringiensis subsp. aizawai ipl durch die expression von insektizidprotein-gen in wirtszellen. Expired - Fee Related DE3682166D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP60242528A JPS62100292A (ja) 1985-10-28 1985-10-28 バチラス・チユリンゲンシス・アイザワイｉｐｌ株の殺虫性蛋白遺伝子
JP61024563A JPS62181784A (ja) 1986-02-06 1986-02-06 殺虫性蛋白遺伝子の大腸菌内発現による殺虫
JP61040925A JP2779440B2 (ja) 1986-02-25 1986-02-25 バチラス・チュリンゲンシス・アイザワイｉｐｌ株の殺虫性蛋白遺伝子

Publications (1)

Publication Number Publication Date
DE3682166D1 true DE3682166D1 (de) 1991-11-28

Family

ID=27284706

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686308180T Expired - Fee Related DE3682166D1 (de) 1985-10-28 1986-10-21 Herstellung von insektizidprotein von bacillus thuringiensis subsp. aizawai ipl durch die expression von insektizidprotein-gen in wirtszellen.

Country Status (3)

Country Link
EP (1) EP0224331B1 (de)
KR (1) KR870004140A (de)
DE (1) DE3682166D1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0817705B2 (ja) * 1986-08-19 1996-02-28 住友化学工業株式会社 Ｂｔ殺虫性蛋白遺伝子
JP3177237B2 (ja) * 1987-06-10 2001-06-18 アンステイテユ・パストウール 鱗翅類に対して幼虫殺虫活性を有するポリペプチドをコードするヌクレオチド配列
FR2616444B1 (fr) * 1987-06-10 1990-03-02 Pasteur Institut Sequences nucleotidiques codant pour des polypeptides dotes d'une activite larvicide vis-a-vis de lepidopteres
DE68922050T2 (de) * 1988-09-06 1995-08-31 Plant Genetic Systems Nv Pflanzen, die mit einer lepidopteralen DNS-Sequenz aus Bazillus thuringiensis transformiert werden.
NZ230375A (en) * 1988-09-09 1991-07-26 Lubrizol Genetics Inc Synthetic gene encoding b. thuringiensis insecticidal protein
TW261517B (de) * 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
FR2740472B1 (fr) * 1995-10-27 1998-01-16 Pasteur Institut Nouvelles souches de bacillus thuringiensis et composition pesticide les contenant

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0795953B2 (ja) * 1981-04-27 1995-10-18 ワシントン・リサ−チ・ファンデ−ション 大腸菌中のバシルス・ツリンギエンシス細晶性蛋白質
FR2525630B1 (fr) * 1982-04-26 1985-07-05 Pasteur Institut Adn contenant une sequence codant pour une proteine du cristal ou un polypeptide doue de proprietes insecticides, micro-organismes transformes par de tels adn, compositions contenant lesdites proteines du cristal, polypeptide ou micro-organismes
US4652628A (en) * 1984-02-22 1987-03-24 Syntro Corporation Methods and compositions for expression of BTI endotoxin
CA1301094C (en) * 1984-08-31 1992-05-19 Helen Riaboff Whiteley Bacillus thuringiensis crystal protein gene toxin segment
JP2540484B2 (ja) * 1984-12-12 1996-10-02 サントリー株式会社 殺虫性蛋白質をコ−ドする遺伝子

Also Published As

Publication number Publication date
EP0224331B1 (de) 1991-10-23
EP0224331A1 (de) 1987-06-03
KR870004140A (ko) 1987-05-07

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee