DE3666707D1 - Immobilization of nucleic acids on derivatized nylon supports - Google Patents

Immobilization of nucleic acids on derivatized nylon supports

Info

Publication number
DE3666707D1
DE3666707D1 DE8686112900T DE3666707T DE3666707D1 DE 3666707 D1 DE3666707 D1 DE 3666707D1 DE 8686112900 T DE8686112900 T DE 8686112900T DE 3666707 T DE3666707 T DE 3666707T DE 3666707 D1 DE3666707 D1 DE 3666707D1
Authority
DE
Germany
Prior art keywords
immobilization
nucleic acids
derivatized nylon
nylon supports
supports
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8686112900T
Other languages
German (de)
English (en)
Inventor
Robert J Carrico
William L Patterson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Inc filed Critical Miles Inc
Application granted granted Critical
Publication of DE3666707D1 publication Critical patent/DE3666707D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE8686112900T 1985-09-30 1986-09-18 Immobilization of nucleic acids on derivatized nylon supports Expired DE3666707D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/781,332 US4806546A (en) 1985-09-30 1985-09-30 Immobilization of nucleic acids on derivatized nylon supports

Publications (1)

Publication Number Publication Date
DE3666707D1 true DE3666707D1 (en) 1989-12-07

Family

ID=25122380

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686112900T Expired DE3666707D1 (en) 1985-09-30 1986-09-18 Immobilization of nucleic acids on derivatized nylon supports

Country Status (5)

Country Link
US (1) US4806546A (https=)
EP (1) EP0221308B1 (https=)
JP (1) JPS6281565A (https=)
CA (1) CA1288709C (https=)
DE (1) DE3666707D1 (https=)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060237A (en) 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
GB2197720A (en) * 1986-11-20 1988-05-25 Nat Res Dev Immobilisation of polynucleotides
DE3850273T2 (de) * 1987-03-02 1994-09-29 Gen Probe Inc Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure.
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
JPH084519B2 (ja) * 1987-12-25 1996-01-24 エフ・ホフマン―ラ ロシュ アーゲー ハイブリッド形成用担体およびその調整方法
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
US4952519A (en) * 1988-05-02 1990-08-28 E. I. Du Pont De Nemours And Company Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group
JP2667447B2 (ja) * 1988-06-28 1997-10-27 オリンパス光学工業株式会社 固体表面への細胞の固定化方法
US5047523A (en) * 1988-08-02 1991-09-10 Ortho Diagnostic Systems Inc. Nucleic acid probe for detection of neisseria gonorrhoea
JP2739068B2 (ja) * 1988-09-24 1998-04-08 春馬 川口 Ｄｎａ固定ミクロスフェア及びそれを用いるｄｎａ転写制御因子精製法
US5972386A (en) * 1995-12-19 1999-10-26 Flinders Technologies Pty, Ltd. Dry solid medium for storage and analysis of genetic material
US6627226B2 (en) 1988-10-05 2003-09-30 Whatman, Inc. Dry solid medium for storage and analysis of genetic material
US6447804B1 (en) 1988-10-05 2002-09-10 Whatman, Plc Dry solid medium for storage and analysis of genetic material
US5807527A (en) * 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US5756126A (en) * 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
WO1990003959A1 (en) * 1988-10-05 1990-04-19 The Flinders University Of South Australia Solid medium and method for dna storage
US5985327A (en) * 1988-10-05 1999-11-16 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US5496562A (en) * 1988-10-05 1996-03-05 Flinders Technologies Pty Ltd Solid medium and method for DNA storage
US5683916A (en) * 1988-10-31 1997-11-04 Hemasure Inc. Membrane affinity apparatus and purification methods related thereto
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
US5030561A (en) * 1988-12-27 1991-07-09 Becton, Dickinson And Company Chlamydia assay using amidine modified supports or particles
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
EP0456765A1 (en) * 1989-01-31 1991-11-21 E.I. Du Pont De Nemours And Company Porous substrates with a high concentration of amine groups
US5352578A (en) * 1989-02-15 1994-10-04 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
US5559221A (en) * 1989-02-15 1996-09-24 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
EP0405913B1 (en) * 1989-06-30 1998-12-23 Chiron Corporation Hydrophobic nucleic acid probe
JPH03257369A (ja) * 1990-02-27 1991-11-15 Yon Rin Mo 簡単なイムノドットニトロセルロースまたはナイロンペーパー
ES2116977T3 (es) * 1990-05-11 1998-08-01 Microprobe Corp Soportes solidos para ensayos de hibridacion de acidos nucleicos y metodos para inmovilizar oligonucleotidos de modo covalente.
US5667976A (en) * 1990-05-11 1997-09-16 Becton Dickinson And Company Solid supports for nucleic acid hybridization assays
US5474895A (en) * 1990-11-14 1995-12-12 Siska Diagnostics Inc. Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
DE69132905T2 (de) * 1990-12-06 2002-08-01 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Detektion von Nukleinsäuresequenzen
FR2679255B1 (fr) * 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
GB9208548D0 (en) * 1992-04-21 1992-06-03 Kodak Ltd An immunoassay and test kit
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6893816B1 (en) * 1993-10-28 2005-05-17 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US20060063193A1 (en) * 1995-04-11 2006-03-23 Dong-Jing Fu Solid phase sequencing of double-stranded nucleic acids
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
WO1996041185A1 (en) * 1995-06-07 1996-12-19 Perseptive Biosystems, Inc. Methods and compositions for binding endotoxins
JPH11514872A (ja) * 1995-10-18 1999-12-21 ダーウィン モレキュラー コーポレイション ハイブリダイゼーション用の固体支持体を調製する方法および非特異的バックグラウンドを低減する方法
CA2248084A1 (en) * 1996-03-04 1997-09-12 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
ES2110916B1 (es) * 1996-05-24 1998-10-01 Ivia Procedimiento de preparacion de dianas para la reaccion en cadena de la polimerasa (pcr).
US5965363A (en) 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
ES2215241T3 (es) 1996-11-06 2004-10-01 Sequenom, Inc. Procedimiento de espectrometria de masa.
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
DE69738206T2 (de) * 1996-11-06 2008-07-17 Sequenom, Inc., San Diego DNA-Diagnostik mittels Massenspektrometrie
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
DE69735445T2 (de) 1996-12-10 2006-08-10 Sequenom, Inc., San Diego Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
JP2001511362A (ja) 1997-07-22 2001-08-14 ラピジーン，インコーポレイテッド Ｍｓにより配列決定データを相関させるコンピュータ方法およびシステム
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6355419B1 (en) 1998-04-27 2002-03-12 Hyseq, Inc. Preparation of pools of nucleic acids based on representation in a sample
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
DE19840531C2 (de) * 1998-08-28 2003-05-15 Roboscreen Ges Fuer Molekulare Mit Nukleinsäuren beschichtete Reaktionsräume, Verfahren zu ihrer Herstellung und ihre Verwendung
CA2348054C (en) * 1998-10-23 2012-06-19 Qiagen Gesellschaft Mit Beschrankter Haftung Processes and means for the isolation and purification of nucleic acids at surfaces
DE69938400T2 (de) * 1998-12-08 2009-04-23 Boston Probes, Inc., Bedford Verfahren, Kits und Zusammensetzungen zur Identifizierung von elektrostatisch an Matrizen gebundenen Nukleinsäuren
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
US6413722B1 (en) 2000-03-22 2002-07-02 Incyte Genomics, Inc. Polymer coated surfaces for microarray applications
AU2001268468A1 (en) 2000-06-13 2001-12-24 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
EP1410001A4 (en) * 2000-10-25 2006-05-31 Michael M Miller MULTI-LAYERED ANALYTICAL ELEMENT AND METHOD OF DETERMINING ANALYTES IN FLUIDS CONTAINING INTERFERING SUBSTANCES
JP3523188B2 (ja) 2000-12-13 2004-04-26 富士写真フイルム株式会社 プローブ分子が固定された検出具の水性処理液
US6818762B2 (en) * 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
US6927029B2 (en) 2001-12-03 2005-08-09 Agilent Technologies, Inc. Surface with tethered polymeric species for binding biomolecules
US20050221283A1 (en) * 2001-12-11 2005-10-06 Mahant Vijay K Biochip
JP2007510928A (ja) * 2003-11-06 2007-04-26 エス アール ユー バイオシステムズ，インコーポレイテッド 高密度アミン機能化表面
CN1980738A (zh) 2004-04-09 2007-06-13 科研智囊团有限公司 用于收集、储存和运输生物样本的设备和方法
WO2007072373A2 (en) * 2005-12-21 2007-06-28 Koninklijke Philips Electronics N.V. A sensor for biomolecules and a method of analysis using said sensor
JP2010508520A (ja) * 2006-10-31 2010-03-18 エス アール ユー バイオシステムズ，インコーポレイテッド 官能基が付与された表面上の非特異的タンパク質結合をブロッキングする方法
US20090137024A1 (en) * 2007-11-28 2009-05-28 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
WO2024092388A1 (zh) * 2022-10-31 2024-05-10 深圳华大智造科技股份有限公司 一种核酸加载试剂及用其检测测序芯片吸附能力的方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1550819A (en) * 1975-06-04 1979-08-22 Nat Res Dev Polymeric support for biogically active materials
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4455370A (en) * 1982-05-17 1984-06-19 E. I. Du Pont De Nemours And Company Transferring separated components in gel electrophoresis via nylon membrane
US4588682A (en) * 1982-12-13 1986-05-13 Integrated Genetics, Inc. Binding nucleic acid to a support
US4512896A (en) * 1983-02-07 1985-04-23 Yale University Transfer of macromolecules from a chromatographic substrate to an immobilizing matrix
IL71715A0 (en) * 1983-08-05 1984-09-30 Miles Lab Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization
US4652517A (en) * 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
FR2565996B1 (fr) * 1984-06-15 1988-01-22 Inst Nat Sante Rech Med Acides nucleiques marques chimiquement, leur utilisation et necessaire pour sa mise en oeuvre
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes

Also Published As

Publication number Publication date
JPH0516838B2 (https=) 1993-03-05
EP0221308A1 (en) 1987-05-13
CA1288709C (en) 1991-09-10
EP0221308B1 (en) 1989-11-02
US4806546A (en) 1989-02-21
JPS6281565A (ja) 1987-04-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee