DE3588008T2 - Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung. - Google Patents

Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung.

Info

Publication number
DE3588008T2
DE3588008T2 DE3588008T DE3588008T DE3588008T2 DE 3588008 T2 DE3588008 T2 DE 3588008T2 DE 3588008 T DE3588008 T DE 3588008T DE 3588008 T DE3588008 T DE 3588008T DE 3588008 T2 DE3588008 T2 DE 3588008T2
Authority
DE
Germany
Prior art keywords
yeast
production
cellulolytic enzymes
yeast strains
cellulolytic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3588008T
Other languages
English (en)
Other versions
DE3588008D1 (de
Inventor
Jonathan Knowles
Merja Penttilae
Tuula Teeri
Helena Nevalainen
Irma Salovuori
Paeivi Lehtovaara-Helenius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AB Enzymes Oy
Original Assignee
Alko Oy AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8518918&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3588008(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Alko Oy AB filed Critical Alko Oy AB
Publication of DE3588008D1 publication Critical patent/DE3588008D1/de
Application granted granted Critical
Publication of DE3588008T2 publication Critical patent/DE3588008T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/945—Trichoderma

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • External Artificial Organs (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE3588008T 1984-04-13 1985-04-12 Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung. Expired - Fee Related DE3588008T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI841500A FI841500A0 (fi) 1984-04-13 1984-04-13 Foerfarande foer uppbygnande av cellulolytiska jaeststammar.
PCT/FI1985/000039 WO1985004672A1 (en) 1984-04-13 1985-04-12 Yeast strains producing cellulolytic enzymes and methods and means for constructing them

Publications (2)

Publication Number Publication Date
DE3588008D1 DE3588008D1 (de) 1995-05-18
DE3588008T2 true DE3588008T2 (de) 1995-10-12

Family

ID=8518918

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3588008T Expired - Fee Related DE3588008T2 (de) 1984-04-13 1985-04-12 Hefestämme zur erzeugung cellulolytischer enzyme und verfahren und mittel zu deren herstellung.

Country Status (8)

Country Link
US (3) US4894338A (de)
EP (2) EP0312121A3 (de)
AT (1) ATE121132T1 (de)
CA (1) CA1305931C (de)
DE (1) DE3588008T2 (de)
DK (1) DK580385A (de)
FI (2) FI841500A0 (de)
WO (1) WO1985004672A1 (de)

Families Citing this family (63)

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US5298405A (en) * 1986-04-30 1994-03-29 Alko Limited Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
US5610034A (en) * 1987-04-29 1997-03-11 Alko Group Ltd. Immunoglobulin production by trichoderma
WO1989009259A1 (en) * 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
US5648263A (en) * 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
US5688290A (en) * 1989-10-19 1997-11-18 Genencor International, Inc. Degradation resistant detergent compositions based on cellulase enzymes
US5866382A (en) 1990-04-06 1999-02-02 Xyrofin Oy Xylose utilization by recombinant yeasts
US5168064A (en) * 1990-04-20 1992-12-01 The Regents Of The University Of California Endo-1,4-β-glucanase gene and its use in plants
NL9001388A (nl) * 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
US5246853A (en) * 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5475101A (en) * 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5650322A (en) * 1990-10-05 1997-07-22 Genencor International, Inc. Methods for stonewashing fabrics using endoglucanases
ES2170748T3 (es) * 1990-10-05 2002-08-16 Genencor Int Procedimientos para el tratamiento de tejidos que contienen algodon con celulasa.
WO1992006183A1 (en) * 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
US5654193A (en) * 1990-10-05 1997-08-05 Genencor International, Inc. Methods for treating cotton containing fabrics with cellulase
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5525507A (en) * 1990-10-05 1996-06-11 Genencor International, Inc. Methods for treating cotton-containing fabric with cellulase composition containing endoglucanase component and which is free of all CBH I component
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
WO1992006221A1 (en) * 1990-10-05 1992-04-16 Genencor International, Inc. Methods for treating cotton-containing fabrics with cellulase
US5443750A (en) * 1991-01-16 1995-08-22 The Procter & Gamble Company Detergent compositions with high activity cellulase and softening clays
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
CA2107208A1 (en) * 1991-03-29 1992-09-30 Kathleen A. Clarkson Methods for treating cotton-containing fabrics with cellulase
US6512166B1 (en) 1991-06-17 2003-01-28 Cornell Research Foundation, Inc. Combinations of fungal cell wall degrading enzyme and fungal cell membrane affecting compound
JPH07506404A (ja) * 1992-05-01 1995-07-13 ジェネンコア　インターナショナル　インコーポレーテッド 綿含有織物をｃｂｈ　ｉが多いセルラーゼで処理する方法
FI92500C (fi) * 1993-03-03 1994-11-25 Valtion Teknillinen Menetelmä mekaanisen massan valmistamiseksi
US7005128B1 (en) 1993-12-17 2006-02-28 Genencor International, Inc. Enzyme feed additive and animal feed including it
US6268196B1 (en) 1993-12-17 2001-07-31 Genencor International, Inc. Method and compositions for treating cellulose containing fabrics using truncated cellulase enzyme compositions
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
ES2251717T3 (es) * 1994-03-08 2006-05-01 Novozymes A/S Nuevas celulasas alcalinas.
US5912157A (en) * 1994-03-08 1999-06-15 Novo Nordisk A/S Alkaline cellulases
US7816129B2 (en) 1994-07-29 2010-10-19 Ab Enzymes Gmbh Production and secretion of proteins of bacterial origin in filamentous fungi
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
US6723549B2 (en) * 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
BR9611648A (pt) * 1995-11-27 1999-02-23 Unilever Nv Composição detergente enzimática
ATE334195T1 (de) * 1996-03-14 2006-08-15 Japan Represented By Director Proteine mit zellulase-aktivitäten und prozess für ihre herstellung
US5789228A (en) * 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
US7465571B1 (en) 1996-05-22 2008-12-16 Verenium Corporation Endoglucanases
ES2242234T3 (es) * 1996-09-12 2005-11-01 Syngenta Participations Ag Plantas transgenicas que expresan enzimas celuloliticas.
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
FI964692A0 (fi) * 1996-11-25 1996-11-25 Primalco Ltd Foerbaettrad cellulassammansaettning foer bioefterbehandling av textilmaterial som innehaoller cellulosa
FI964691A0 (fi) * 1996-11-25 1996-11-25 Primalco Ltd Foerbaettrad cellulassammasaettning foer behandling av textilmaterial som innehaoller cellulosa
US6001595A (en) * 1996-11-29 1999-12-14 Rohm Enzyme GmbH Promoters and uses thereof
US6818803B1 (en) 1997-06-26 2004-11-16 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
US6558937B1 (en) 1997-07-31 2003-05-06 Dsm N.V. Cellulose degrading enzymes of aspergillus
DE69827712T3 (de) * 1997-07-31 2009-07-09 Dsm Ip Assets B.V. Zusammensetzung für Brotverbesserung
US6222028B1 (en) 1999-10-15 2001-04-24 Academia Sinica Polynucleotides encoding cellulase enzymes from Piromyces rhizinflata
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EP1320588B2 (de) * 2000-09-25 2017-06-28 Iogen Energy Corporation Verfahren zur herstellung von glucose mit ein ermodifizierten zellulase
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EP1485495B1 (de) * 2002-03-15 2010-09-15 Iogen Energy Corporation Verfahren zur herstellung von glukose durch verwendung von endoglucanase-core-protein zur verbesserten rückgewinnung und wiederverwendung des enzyms
ATE438711T1 (de) 2002-12-20 2009-08-15 Novozymes As Polypeptide mit cellobiohydrolase ii-aktivität und dafür kodierende polynucleotide
EP1730284A4 (de) * 2004-03-08 2008-04-30 Syngenta Participations Ag Selbstprozessierende pflanzen und pflanzenteile
ATE447013T1 (de) * 2005-04-27 2009-11-15 Novozymes Inc Polypeptide mit endoglucanase-aktivtät und dafür codierende polynukleotide
NZ610301A (en) * 2007-01-30 2015-03-27 Bp Corp North America Inc Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
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EP3235903B1 (de) * 2011-01-26 2021-07-07 Novozymes A/S Polypeptide mit cellobiohydrolaseaktivität and dafür kodierende polynukleotide
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
US8778639B1 (en) * 2013-02-12 2014-07-15 Novozymes Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
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FR3022558B1 (fr) * 2014-06-20 2019-01-25 Proteus Variants d'exoglucanases a activite amelioree et leurs utilisations
CN113322190B (zh) * 2021-06-24 2023-07-18 西北农林科技大学 里氏木霉与酿酒酵母混合发酵白酒糟生产单细胞蛋白的方法

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US4275163A (en) * 1978-11-20 1981-06-23 The United States Of America As Represented By The Secretary Of The Army Cellulase-producing microorganism
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Also Published As

Publication number Publication date
EP0214971B1 (de) 1995-04-12
DE3588008D1 (de) 1995-05-18
FI864110A (fi) 1986-10-10
FI864110A0 (fi) 1986-10-10
FI86745C (fi) 1992-10-12
CA1305931C (en) 1992-08-04
EP0312121A3 (de) 1989-12-13
US5393670A (en) 1995-02-28
DK580385D0 (da) 1985-12-13
FI841500A0 (fi) 1984-04-13
FI86745B (fi) 1992-06-30
US4894338A (en) 1990-01-16
EP0214971A1 (de) 1987-03-25
ATE121132T1 (de) 1995-04-15
WO1985004672A1 (en) 1985-10-24
EP0312121A2 (de) 1989-04-19
DK580385A (da) 1985-12-13
US5529919A (en) 1996-06-25

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Owner name: ROEHM ENZYME FINLAND OY, HELSINKI, FI

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