DE3575576D1 - Mikroinjektionstechnik fuer pflanzenzellen. - Google Patents

Mikroinjektionstechnik fuer pflanzenzellen.

Info

Publication number
DE3575576D1
DE3575576D1 DE8585111126T DE3575576T DE3575576D1 DE 3575576 D1 DE3575576 D1 DE 3575576D1 DE 8585111126 T DE8585111126 T DE 8585111126T DE 3575576 T DE3575576 T DE 3575576T DE 3575576 D1 DE3575576 D1 DE 3575576D1
Authority
DE
Germany
Prior art keywords
protoplasts
injection
plant cells
cell wall
pipette
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8585111126T
Other languages
English (en)
Inventor
Anne Crossway
Daniel Facciotti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Calgene LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene LLC filed Critical Calgene LLC
Application granted granted Critical
Publication of DE3575576D1 publication Critical patent/DE3575576D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DE8585111126T 1984-09-25 1985-09-04 Mikroinjektionstechnik fuer pflanzenzellen. Expired - Lifetime DE3575576D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US65460684A 1984-09-25 1984-09-25

Publications (1)

Publication Number Publication Date
DE3575576D1 true DE3575576D1 (de) 1990-03-01

Family

ID=24625541

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585111126T Expired - Lifetime DE3575576D1 (de) 1984-09-25 1985-09-04 Mikroinjektionstechnik fuer pflanzenzellen.

Country Status (5)

Country Link
EP (1) EP0175966B1 (de)
JP (1) JPS6178388A (de)
AT (1) ATE49778T1 (de)
CA (1) CA1280081C (de)
DE (1) DE3575576D1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH670655A5 (de) * 1987-01-29 1989-06-30 Inst Botan Im N G Kholodnogo A
BE1000348A5 (fr) * 1987-02-27 1988-11-08 Inst Botan Im N G Kholodnogo A Procede d'obtention d'objets vegetaux genetiquement transformes.
ZA901241B (en) * 1989-02-17 1991-10-30 United Agriseeds Inc Method for increasing the ability of maize to undergo androgenesis,and products produced therefrom
JPH0463597A (ja) * 1990-06-29 1992-02-28 W R Grace & Co 細胞への遺伝子導入法およびそれに用いる細胞固定板
CA2275474A1 (en) * 1996-12-20 1998-07-02 Genesystems, Inc. Method and device for microinjection of macromolecules into non-adherent cells
GB0002814D0 (en) 2000-02-09 2000-03-29 Univ York Nucleic acids and their uses
EP1551967B1 (de) 2002-07-19 2011-08-31 University Of South Carolina Zusammensetzungen und verfahren zur modulation der genexpression in pflanzen
ATE405658T1 (de) 2002-07-26 2008-09-15 Basf Plant Science Gmbh Neue selektionsverfahren
EP1502954A1 (de) 2003-07-31 2005-02-02 greenovation Biotech GmbH Gebrauch von Konstrukten mit rekombinanten Sequenzmotifen zur Erhöhung der Genexpression in Moosen
CA2541294C (en) 2003-11-10 2013-06-25 Icon Genetics Ag Rna virus-derived plant expression system
EP1616959A1 (de) 2004-07-07 2006-01-18 Icon Genetics AG Biologisch sichere transiente Proteinexpression in Pflanzen
CA2681661C (en) 2007-03-23 2015-11-24 New York University Methods of increasing nitrogen-assimilation capacity in transgenic plants expressing cca1 and glk1
GB0709835D0 (en) 2007-05-22 2007-07-04 Plant Bioscience Ltd Composition and method for modulating plant root hair development
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
GB2465748B (en) 2008-11-25 2012-04-25 Algentech Sas Plant cell transformation method
GB2467167B (en) 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
US20120174263A1 (en) 2009-06-15 2012-07-05 Plant Bioscience Limited Production of viral capsids
EP2462222B1 (de) 2009-08-05 2017-05-24 Chromocell Corporation Verbesserte pflanzen, mikroben und organismen
EP2357239A1 (de) 2009-10-29 2011-08-17 Universität zu Köln Verfahren und Mittel für ein auswählbares Markierungssystem bei Pflanzen
GB201008720D0 (en) 2010-05-25 2010-07-07 Univ Aberdeen RxLR-leader peptides and protein translocation
JP6025214B2 (ja) 2010-11-04 2016-11-16 メディカゴ インコーポレイテッド 植物発現系
AU2014234251A1 (en) 2013-03-22 2015-06-18 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Kits comprising plus-sense single stranded RNA viral vectors and methods for producing polypeptides using the kits
EP3470420A1 (de) 2013-08-14 2019-04-17 Institute Of Genetics And Developmental Biology Verfahren zur modulation der samen- und organgrösse bei pflanzen
GB201319876D0 (en) 2013-11-11 2013-12-25 Plant Bioscience Ltd Methods of modulating seed and organ size in plants
WO2015143567A1 (en) 2014-03-27 2015-10-01 Medicago Inc. Modified cpmv enhancer elements
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
AU2016287799A1 (en) 2015-07-02 2018-01-04 Medicago Inc. Jasmonic acid pathway activator
EP3260542A1 (de) 2016-06-20 2017-12-27 Algentech Proteinproduktion in pflanzenzellen
WO2019185129A1 (en) 2018-03-27 2019-10-03 The University Court Of The University Of Glasgow Bacterial pathogen derived resistance in plants
USD1003864S1 (en) 2019-04-01 2023-11-07 Alpine Electronics, Inc. Speaker surround
WO2022260849A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods and systems for producing a protein of interest in a plant

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001176A1 (en) * 1981-10-01 1983-04-14 Int Plant Research Inst Process for the genetic modification of cereals with transformation vectors

Also Published As

Publication number Publication date
EP0175966A1 (de) 1986-04-02
CA1280081C (en) 1991-02-12
ATE49778T1 (de) 1990-02-15
EP0175966B1 (de) 1990-01-24
JPS6178388A (ja) 1986-04-21

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee