ATE49778T1 - Mikroinjektionstechnik fuer pflanzenzellen. - Google Patents
Mikroinjektionstechnik fuer pflanzenzellen.Info
- Publication number
- ATE49778T1 ATE49778T1 AT85111126T AT85111126T ATE49778T1 AT E49778 T1 ATE49778 T1 AT E49778T1 AT 85111126 T AT85111126 T AT 85111126T AT 85111126 T AT85111126 T AT 85111126T AT E49778 T1 ATE49778 T1 AT E49778T1
- Authority
- AT
- Austria
- Prior art keywords
- protoplasts
- injection
- plant cells
- cell wall
- pipette
- Prior art date
Links
- 238000000520 microinjection Methods 0.000 title abstract 2
- 210000001938 protoplast Anatomy 0.000 abstract 5
- 238000002347 injection Methods 0.000 abstract 3
- 239000007924 injection Substances 0.000 abstract 3
- 210000002421 cell wall Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000008929 regeneration Effects 0.000 abstract 1
- 238000011069 regeneration method Methods 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US65460684A | 1984-09-25 | 1984-09-25 | |
| EP85111126A EP0175966B1 (de) | 1984-09-25 | 1985-09-04 | Mikroinjektionstechnik für Pflanzenzellen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE49778T1 true ATE49778T1 (de) | 1990-02-15 |
Family
ID=24625541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT85111126T ATE49778T1 (de) | 1984-09-25 | 1985-09-04 | Mikroinjektionstechnik fuer pflanzenzellen. |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0175966B1 (de) |
| JP (1) | JPS6178388A (de) |
| AT (1) | ATE49778T1 (de) |
| CA (1) | CA1280081C (de) |
| DE (1) | DE3575576D1 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2200367B (en) * | 1987-01-29 | 1990-08-08 | Inst Botan Im N G Kholodnogo A | Method of preparing genetically transformed cellular plant matter by micro- injection. |
| BE1000348A5 (fr) * | 1987-02-27 | 1988-11-08 | Inst Botan Im N G Kholodnogo A | Procede d'obtention d'objets vegetaux genetiquement transformes. |
| ZA901241B (en) * | 1989-02-17 | 1991-10-30 | United Agriseeds Inc | Method for increasing the ability of maize to undergo androgenesis,and products produced therefrom |
| JPH0463597A (ja) * | 1990-06-29 | 1992-02-28 | W R Grace & Co | 細胞への遺伝子導入法およびそれに用いる細胞固定板 |
| EP0948594A1 (de) * | 1996-12-20 | 1999-10-13 | Gene-Cell | Verfahren und vorrichtung zur mikroinjektion von nicht-adhaerenten zellen |
| GB0002814D0 (en) | 2000-02-09 | 2000-03-29 | Univ York | Nucleic acids and their uses |
| ATE522130T1 (de) | 2002-07-19 | 2011-09-15 | Univ South Carolina | Zusammensetzungen und verfahren zur modulation der genexpression in pflanzen |
| AU2003250115B2 (en) | 2002-07-26 | 2009-03-12 | Basf Plant Science Gmbh | Inversion of the negative-selective effect of negative marker proteins using selection methods |
| EP1502954A1 (de) | 2003-07-31 | 2005-02-02 | greenovation Biotech GmbH | Gebrauch von Konstrukten mit rekombinanten Sequenzmotifen zur Erhöhung der Genexpression in Moosen |
| DK1682667T3 (da) | 2003-11-10 | 2010-08-23 | Icon Genetics Gmbh | RNA-virus afledt plantekspressionssystem |
| EP1616959A1 (de) | 2004-07-07 | 2006-01-18 | Icon Genetics AG | Biologisch sichere transiente Proteinexpression in Pflanzen |
| US8153863B2 (en) | 2007-03-23 | 2012-04-10 | New York University | Transgenic plants expressing GLK1 and CCA1 having increased nitrogen assimilation capacity |
| GB0709835D0 (en) | 2007-05-22 | 2007-07-04 | Plant Bioscience Ltd | Composition and method for modulating plant root hair development |
| GB2465748B (en) | 2008-11-25 | 2012-04-25 | Algentech Sas | Plant cell transformation method |
| GB2465749B (en) | 2008-11-25 | 2013-05-08 | Algentech Sas | Plant cell transformation method |
| GB2467167B (en) | 2009-01-26 | 2013-09-11 | Algentech Sas | Gene targeting in plants |
| US20120174263A1 (en) | 2009-06-15 | 2012-07-05 | Plant Bioscience Limited | Production of viral capsids |
| CA2769651A1 (en) | 2009-08-05 | 2011-02-10 | Chromocell Corporation | Improved plants, microbes, and organisms |
| EP2357239A1 (de) | 2009-10-29 | 2011-08-17 | Universität zu Köln | Verfahren und Mittel für ein auswählbares Markierungssystem bei Pflanzen |
| GB201008720D0 (en) | 2010-05-25 | 2010-07-07 | Univ Aberdeen | RxLR-leader peptides and protein translocation |
| CA2815887C (en) | 2010-11-04 | 2013-12-10 | Medicago Inc. | Plant expression system |
| US10190128B2 (en) | 2013-03-22 | 2019-01-29 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Kits comprising plus-sense single stranded RNA viral vectors and methods for producing polypeptides using the kits |
| EP4667564A2 (de) | 2013-08-14 | 2025-12-24 | Institute Of Genetics And Developmental Biology, Chinese Academy of Sciences | Verfahren zur modulation der samen- und organgrösse bei pflanzen |
| GB201319876D0 (en) | 2013-11-11 | 2013-12-25 | Plant Bioscience Ltd | Methods of modulating seed and organ size in plants |
| AU2015234595B2 (en) | 2014-03-27 | 2021-03-25 | Medicago Inc. | Modified CPMV enhancer elements |
| RU2731295C2 (ru) | 2014-07-11 | 2020-09-01 | Медикаго Инк. | Модификация продуцирования белков в растениях |
| MX2017016366A (es) | 2015-07-02 | 2018-05-22 | Medicago Inc | Activador del ciclo de acido jasmonico. |
| EP3260542A1 (de) | 2016-06-20 | 2017-12-27 | Algentech | Proteinproduktion in pflanzenzellen |
| WO2019185129A1 (en) | 2018-03-27 | 2019-10-03 | The University Court Of The University Of Glasgow | Bacterial pathogen derived resistance in plants |
| USD1003864S1 (en) | 2019-04-01 | 2023-11-07 | Alpine Electronics, Inc. | Speaker surround |
| CN117479831A (zh) | 2021-06-09 | 2024-01-30 | 南特知识产权控股有限责任公司 | 用于在植物中生产感兴趣的蛋白质的方法和系统 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0090033A1 (de) * | 1981-10-01 | 1983-10-05 | International Plant Research Institute | Verfahren für die genetische modifizierung von getreiden mit tranformatorvektoren |
-
1985
- 1985-08-22 CA CA000489269A patent/CA1280081C/en not_active Expired - Lifetime
- 1985-09-04 DE DE8585111126T patent/DE3575576D1/de not_active Expired - Lifetime
- 1985-09-04 EP EP85111126A patent/EP0175966B1/de not_active Expired - Lifetime
- 1985-09-04 AT AT85111126T patent/ATE49778T1/de not_active IP Right Cessation
- 1985-09-13 JP JP60201958A patent/JPS6178388A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA1280081C (en) | 1991-02-12 |
| EP0175966A1 (de) | 1986-04-02 |
| DE3575576D1 (de) | 1990-03-01 |
| EP0175966B1 (de) | 1990-01-24 |
| JPS6178388A (ja) | 1986-04-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |