DE3511327A1 - Stabilisierung der aktivitaet von peroxidase in loesung - Google Patents

Stabilisierung der aktivitaet von peroxidase in loesung

Info

Publication number
DE3511327A1
DE3511327A1 DE19853511327 DE3511327A DE3511327A1 DE 3511327 A1 DE3511327 A1 DE 3511327A1 DE 19853511327 DE19853511327 DE 19853511327 DE 3511327 A DE3511327 A DE 3511327A DE 3511327 A1 DE3511327 A1 DE 3511327A1
Authority
DE
Germany
Prior art keywords
solution
activity
peroxidase
stabilizer
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19853511327
Other languages
German (de)
English (en)
Inventor
Rolf Dr.rer.nat. 8139 Bernried Deeg
Gerd Dr.Rer.Nat. 8132 Tutzing Kleinhammer
Dagmar Dr.rer.nat. Klenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE19853511327 priority Critical patent/DE3511327A1/de
Priority to US06/835,896 priority patent/US4757016A/en
Priority to DE8686104215T priority patent/DE3665320D1/de
Priority to EP86104215A priority patent/EP0197447B1/de
Priority to AT86104215T priority patent/ATE45978T1/de
Priority to JP61068899A priority patent/JPS61224987A/ja
Publication of DE3511327A1 publication Critical patent/DE3511327A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Enzymes And Modification Thereof (AREA)
DE19853511327 1985-03-28 1985-03-28 Stabilisierung der aktivitaet von peroxidase in loesung Withdrawn DE3511327A1 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE19853511327 DE3511327A1 (de) 1985-03-28 1985-03-28 Stabilisierung der aktivitaet von peroxidase in loesung
US06/835,896 US4757016A (en) 1985-03-28 1986-03-04 Process for stabilizing the activity of peroxidase in solution
DE8686104215T DE3665320D1 (en) 1985-03-28 1986-03-26 Stabilization of peroxidase activity in solution
EP86104215A EP0197447B1 (de) 1985-03-28 1986-03-26 Stabilisierung der Aktivität von Peroxidase in Lösung
AT86104215T ATE45978T1 (de) 1985-03-28 1986-03-26 Stabilisierung der aktivitaet von peroxidase in loesung.
JP61068899A JPS61224987A (ja) 1985-03-28 1986-03-28 溶液中のペルオキシダーゼの活性を安定化する方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853511327 DE3511327A1 (de) 1985-03-28 1985-03-28 Stabilisierung der aktivitaet von peroxidase in loesung

Publications (1)

Publication Number Publication Date
DE3511327A1 true DE3511327A1 (de) 1986-10-02

Family

ID=6266621

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19853511327 Withdrawn DE3511327A1 (de) 1985-03-28 1985-03-28 Stabilisierung der aktivitaet von peroxidase in loesung
DE8686104215T Expired DE3665320D1 (en) 1985-03-28 1986-03-26 Stabilization of peroxidase activity in solution

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8686104215T Expired DE3665320D1 (en) 1985-03-28 1986-03-26 Stabilization of peroxidase activity in solution

Country Status (5)

Country Link
US (1) US4757016A (https=)
EP (1) EP0197447B1 (https=)
JP (1) JPS61224987A (https=)
AT (1) ATE45978T1 (https=)
DE (2) DE3511327A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874692A (en) * 1987-07-20 1989-10-17 Eastman Kodak Company Binder composition and analytical element having stabilized peroxidase in layer containing the composition
FR2630457B1 (fr) * 1988-04-20 1990-08-31 Anda Biolog Utilisation de l'acide para-amino-salicylique pour la stabilisation de l'activite enzymatique de la peroxydase en solution, agent stabilisant et solution stabilisee en comportant application
US5116729A (en) * 1989-03-10 1992-05-26 Miles Inc. Stabilization of oxidase enzyme-based test strips
FR2653782B1 (fr) * 1989-10-30 1992-02-07 Cis Bio Int Composition de peroxydase stabilisee, utilisable pour des essais immuno-enzymatiques.
US5468622A (en) * 1990-04-03 1995-11-21 Immunomatrix, Inc. Salt stabilization of antibody-enzyme conjugates heat-dried into paper
DE69228817T2 (de) * 1991-07-26 1999-09-23 Dade Chemistry Systems Inc., Deerfield Signalerkennungspruefung in der anwesenheit von einem suspendierten festen träger
US5460944A (en) * 1991-10-28 1995-10-24 Boehringer Mannheim Gmbh Storable protein solution
WO2006076034A2 (en) * 2004-05-21 2006-07-20 Bhanu, Kalra Stabilized two component system for chemilumiescent assay in immunodiagnostics
CN106399322A (zh) * 2016-08-27 2017-02-15 华南农业大学 一种鸡β干扰素优化基因及重组鸡β干扰素的生产工艺

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL7610608A (nl) * 1976-09-24 1978-03-29 Akzo Nv Werkwijze voor het stabiliseren van peroxidase- -bevattende composities.
ATE5539T1 (de) * 1979-08-23 1983-12-15 Ivan Endre Modrovich Verfahren zur stabilisierung einer enzymatischen loesung zur verwendung bei der bestimmung des gesamt-cholesterols, stabilisierte loesung und reagenzsatz dafuer.
US4378429A (en) * 1979-08-23 1983-03-29 Modrovich Ivan Endre Enzymatic method and stabilized solutions for determining total cholesterol in human serum
US4252896A (en) * 1980-01-07 1981-02-24 Abbott Laboratories Method of stabilizing peroxidase in a serum protein based medium
IT1130252B (it) * 1980-02-04 1986-06-11 Elvi Spa Metodo per l'eliminazione dell'interferenza da biliribuna nel dosaggio di perossido di idrigeno mediante una reazione di trinder modificata
DE3261832D1 (en) * 1981-07-30 1985-02-21 Hoffmann La Roche Method for the stabilisation of peroxidase in a liquid medium, and the stabilised medium so obtained
US4504579A (en) * 1982-11-08 1985-03-12 Abbott Laboratories Stabilized peroxidase compositions

Also Published As

Publication number Publication date
EP0197447B1 (de) 1989-08-30
JPS61224987A (ja) 1986-10-06
DE3665320D1 (en) 1989-10-05
US4757016A (en) 1988-07-12
JPH0129556B2 (https=) 1989-06-12
EP0197447A1 (de) 1986-10-15
ATE45978T1 (de) 1989-09-15

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Legal Events

Date Code Title Description
8139 Disposal/non-payment of the annual fee