DE3381980D1 - Verfahren zur halbsynthese von menschlichem insulin und dazu zu verwendende alkalische protease. - Google Patents

Verfahren zur halbsynthese von menschlichem insulin und dazu zu verwendende alkalische protease.

Info

Publication number
DE3381980D1
DE3381980D1 DE8383104036T DE3381980T DE3381980D1 DE 3381980 D1 DE3381980 D1 DE 3381980D1 DE 8383104036 T DE8383104036 T DE 8383104036T DE 3381980 T DE3381980 T DE 3381980T DE 3381980 D1 DE3381980 D1 DE 3381980D1
Authority
DE
Germany
Prior art keywords
synthesis
semi
human insulin
alkaline protease
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8383104036T
Other languages
English (en)
Inventor
Masami Soejima
Takeharu Masaki
Hideya Suzuki
Jyoji Nagaoka
Yoshitsugu Sakata
Akinori Shintani
Nobuaki Minamii
Tetsuya Matsuo
Haruhiko Sugiyama
Nobuyuki Tokioka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP6823882A external-priority patent/JPS58187186A/ja
Priority claimed from JP12391782A external-priority patent/JPS5914798A/ja
Priority claimed from JP57162976A external-priority patent/JPS5951789A/ja
Priority claimed from JP57169428A external-priority patent/JPS5959189A/ja
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Application granted granted Critical
Publication of DE3381980D1 publication Critical patent/DE3381980D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DE8383104036T 1982-04-23 1983-04-25 Verfahren zur halbsynthese von menschlichem insulin und dazu zu verwendende alkalische protease. Expired - Fee Related DE3381980D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP6823882A JPS58187186A (ja) 1982-04-23 1982-04-23 固定化酵素
JP12391782A JPS5914798A (ja) 1982-07-16 1982-07-16 ヒトインシユリンの半合成法
JP57162976A JPS5951789A (ja) 1982-09-18 1982-09-18 安定化酵素
JP57169428A JPS5959189A (ja) 1982-09-28 1982-09-28 新規なアルカリプロテア−ゼ

Publications (1)

Publication Number Publication Date
DE3381980D1 true DE3381980D1 (de) 1990-12-13

Family

ID=27464963

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8383104036T Expired - Fee Related DE3381980D1 (de) 1982-04-23 1983-04-25 Verfahren zur halbsynthese von menschlichem insulin und dazu zu verwendende alkalische protease.

Country Status (3)

Country Link
EP (2) EP0092829B1 (de)
DE (1) DE3381980D1 (de)
DK (2) DK182483A (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801022A (en) * 1990-08-03 1998-09-01 Vertex Pharmaceuticals, Incorporated Method of producing a product with crosslinked crystals of thermolysin
ATE323158T1 (de) * 1990-08-03 2006-04-15 Vertex Pharma Verwendung von vernetzten kristallen als neue form von immobilisierung von enzymen
US5618710A (en) * 1990-08-03 1997-04-08 Vertex Pharmaceuticals, Inc. Crosslinked enzyme crystals
AU2091397A (en) * 1996-03-12 1997-10-01 Novo Nordisk A/S Novel achromobacter lyticus protease variants
US7396903B2 (en) * 2001-11-19 2008-07-08 Novo Nordisk A/S Process for preparing insulin compounds

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH453269A4 (de) * 1968-03-29 1973-01-31
US4251631A (en) * 1978-02-23 1981-02-17 Research Products Rehovot Ltd. Cross-linked enzyme membrane
JPS54119085A (en) * 1978-03-09 1979-09-14 Takeda Chem Ind Ltd Alkaliprotease
NO153061C (no) * 1979-04-13 1986-01-08 Shionogi & Co Fremgangsmaate for fremstilling av et b30-threonininsulin
DK147437A (en) * 1980-02-11 1900-01-01 Process for preparing human insulin or threonine B30 esters of human insulin, or a salt or complex thereof
DE3101382A1 (de) * 1981-01-17 1982-09-02 Hoechst Ag, 6000 Frankfurt "verfahren zur herstellung von humanisulin oder dessen derivaten aus schweineinsulin oder dessen derivaten"
WO1982004069A1 (en) * 1981-05-20 1982-11-25 Andresen Finn Hede A process for the preparation of insulin derivatives
EP0085083B1 (de) * 1981-08-10 1985-01-23 Nordisk Insulinlaboratorium Verfahren zur enzymatischen Herstellung von Humaninsulin
US4601979A (en) * 1981-09-15 1986-07-22 Nordisk Insulinlaboratorium Process for preparing human insulin or B-30 esters thereof

Also Published As

Publication number Publication date
DK182483A (da) 1983-10-24
DK152791A (da) 1991-08-29
EP0092829A3 (en) 1985-08-21
EP0092829A2 (de) 1983-11-02
EP0367302A3 (de) 1991-06-19
EP0367302A2 (de) 1990-05-09
DK152791D0 (da) 1991-08-29
DK182483D0 (da) 1983-04-25
EP0092829B1 (de) 1990-11-07

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee