DE3048884A1 - Verfahren zur enzymimmunobestimmung in heterogener phase - Google Patents
Verfahren zur enzymimmunobestimmung in heterogener phaseInfo
- Publication number
- DE3048884A1 DE3048884A1 DE19803048884 DE3048884A DE3048884A1 DE 3048884 A1 DE3048884 A1 DE 3048884A1 DE 19803048884 DE19803048884 DE 19803048884 DE 3048884 A DE3048884 A DE 3048884A DE 3048884 A1 DE3048884 A1 DE 3048884A1
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- component
- liquid phase
- binding
- insoluble
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 38
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000007790 solid phase Substances 0.000 claims abstract description 15
- 230000027455 binding Effects 0.000 claims abstract description 14
- 239000007791 liquid phase Substances 0.000 claims abstract description 14
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims abstract description 9
- 239000012488 sample solution Substances 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 7
- 230000009471 action Effects 0.000 claims abstract description 6
- 238000011534 incubation Methods 0.000 claims abstract description 6
- 108091008324 binding proteins Proteins 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 5
- 238000005259 measurement Methods 0.000 claims abstract description 4
- 102000014914 Carrier Proteins Human genes 0.000 claims abstract 3
- 239000004816 latex Substances 0.000 claims description 9
- 229920000126 latex Polymers 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 9
- 230000005484 gravity Effects 0.000 claims description 8
- 239000000427 antigen Substances 0.000 claims description 5
- 102000036639 antigens Human genes 0.000 claims description 5
- 108091007433 antigens Proteins 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000002372 labelling Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 101100072419 Caenorhabditis elegans ins-6 gene Proteins 0.000 claims 1
- 239000003550 marker Substances 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 230000009870 specific binding Effects 0.000 abstract description 5
- 229940088598 enzyme Drugs 0.000 description 28
- 239000000243 solution Substances 0.000 description 16
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 6
- 238000002965 ELISA Methods 0.000 description 5
- 229920002684 Sepharose Polymers 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 108010015776 Glucose oxidase Proteins 0.000 description 3
- 239000004366 Glucose oxidase Substances 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 238000004132 cross linking Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 229940116332 glucose oxidase Drugs 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- PCDWFBFHIIKIPM-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole-2-sulfonic acid Chemical compound C1=CC=C2N(CC)C(S(O)(=O)=O)SC2=C1 PCDWFBFHIIKIPM-UHFFFAOYSA-N 0.000 description 2
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 2
- 102000023732 binding proteins Human genes 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- 238000007720 emulsion polymerization reaction Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000941 radioactive substance Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- KZNICNPSHKQLFF-UHFFFAOYSA-N succinimide Chemical group O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 229940034208 thyroxine Drugs 0.000 description 2
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- NNJPGOLRFBJNIW-HNNXBMFYSA-N (-)-demecolcine Chemical compound C1=C(OC)C(=O)C=C2[C@@H](NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=C1 NNJPGOLRFBJNIW-HNNXBMFYSA-N 0.000 description 1
- PROQIPRRNZUXQM-UHFFFAOYSA-N (16alpha,17betaOH)-Estra-1,3,5(10)-triene-3,16,17-triol Natural products OC1=CC=C2C3CCC(C)(C(C(O)C4)O)C4C3CCC2=C1 PROQIPRRNZUXQM-UHFFFAOYSA-N 0.000 description 1
- 102000012440 Acetylcholinesterase Human genes 0.000 description 1
- 108010022752 Acetylcholinesterase Proteins 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 1
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 1
- 102000008857 Ferritin Human genes 0.000 description 1
- 108050000784 Ferritin Proteins 0.000 description 1
- 238000008416 Ferritin Methods 0.000 description 1
- 102000004641 Fetal Proteins Human genes 0.000 description 1
- 108010003471 Fetal Proteins Proteins 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 241000473945 Theria <moth genus> Species 0.000 description 1
- 102000011923 Thyrotropin Human genes 0.000 description 1
- 108010061174 Thyrotropin Proteins 0.000 description 1
- 229940022698 acetylcholinesterase Drugs 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- PROQIPRRNZUXQM-ZXXIGWHRSA-N estriol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H]([C@H](O)C4)O)[C@@H]4[C@@H]3CCC2=C1 PROQIPRRNZUXQM-ZXXIGWHRSA-N 0.000 description 1
- 229960001348 estriol Drugs 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229920001480 hydrophilic copolymer Polymers 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- -1 p-nitrophenyl- Chemical group 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 229960000874 thyrotropin Drugs 0.000 description 1
- 230000001748 thyrotropin Effects 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/971—Capture of complex after antigen-antibody reaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Compounds Of Unknown Constitution (AREA)
- Saccharide Compounds (AREA)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803048884 DE3048884A1 (de) | 1980-12-23 | 1980-12-23 | Verfahren zur enzymimmunobestimmung in heterogener phase |
| DE8181108754T DE3170532D1 (en) | 1980-12-23 | 1981-10-22 | Process for enzyme immuno-determination in the heterogeneous phase |
| EP81108754A EP0054675B1 (de) | 1980-12-23 | 1981-10-22 | Verfahren zur Enzymimmunobestimmung in heterogener Phase |
| AT81108754T ATE13356T1 (de) | 1980-12-23 | 1981-10-22 | Verfahren zur enzymimmunobestimmung in heterogener phase. |
| CA000391389A CA1170180A (en) | 1980-12-23 | 1981-12-02 | Process for the enzyme immuno determination in the heterogenous phase |
| ES507817A ES8302312A1 (es) | 1980-12-23 | 1981-12-09 | Procedimiento inmunoenzimatico en fase hetereogenea para la determinacion de componentes. |
| DD81235744A DD201943A5 (de) | 1980-12-23 | 1981-12-14 | Verfahren zur enzymimmonolbestimmung in heterogener phase |
| US06/331,339 US4743536A (en) | 1980-12-23 | 1981-12-16 | Method for enzyme immuno-determinations in heterogeneous phase |
| AU78668/81A AU524904B2 (en) | 1980-12-23 | 1981-12-18 | Enzyme immunodetermination in heterogenous phase |
| JP56206303A JPS57132060A (en) | 1980-12-23 | 1981-12-22 | Peculiarly bonding protein and method of measuring one component in reaction of material able to be bonded thereto |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803048884 DE3048884A1 (de) | 1980-12-23 | 1980-12-23 | Verfahren zur enzymimmunobestimmung in heterogener phase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3048884A1 true DE3048884A1 (de) | 1982-07-15 |
Family
ID=6120186
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19803048884 Withdrawn DE3048884A1 (de) | 1980-12-23 | 1980-12-23 | Verfahren zur enzymimmunobestimmung in heterogener phase |
| DE8181108754T Expired DE3170532D1 (en) | 1980-12-23 | 1981-10-22 | Process for enzyme immuno-determination in the heterogeneous phase |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8181108754T Expired DE3170532D1 (en) | 1980-12-23 | 1981-10-22 | Process for enzyme immuno-determination in the heterogeneous phase |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US4743536A (en, 2012) |
| EP (1) | EP0054675B1 (en, 2012) |
| JP (1) | JPS57132060A (en, 2012) |
| AT (1) | ATE13356T1 (en, 2012) |
| AU (1) | AU524904B2 (en, 2012) |
| CA (1) | CA1170180A (en, 2012) |
| DD (1) | DD201943A5 (en, 2012) |
| DE (2) | DE3048884A1 (en, 2012) |
| ES (1) | ES8302312A1 (en, 2012) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60152954A (ja) * | 1984-01-20 | 1985-08-12 | Hitachi Ltd | 生体試料の測定方法 |
| JPS60152953A (ja) * | 1984-01-20 | 1985-08-12 | Hitachi Ltd | 免疫測定用分析装置 |
| JPH0799369B2 (ja) * | 1985-11-25 | 1995-10-25 | 日本ケミカルリサーチ株式会社 | 上皮細胞増殖因子の酵素免疫測定法 |
| JPH06105252B2 (ja) * | 1986-02-05 | 1994-12-21 | 三共株式会社 | 遊離型サイロキシンの酵素免疫測定法 |
| JPH0750112B2 (ja) * | 1988-04-26 | 1995-05-31 | 日本電信電話株式会社 | レーザ磁気免疫測定方法を実施するための検体調整方法 |
| DE3941150A1 (de) * | 1989-12-13 | 1991-06-20 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung eines analyten |
| US5364591A (en) * | 1992-06-01 | 1994-11-15 | Eastman Kodak Company | Device for moving a target-bearing solid through a liquid for detection while being contained |
| US5795784A (en) | 1996-09-19 | 1998-08-18 | Abbott Laboratories | Method of performing a process for determining an item of interest in a sample |
| US5856194A (en) | 1996-09-19 | 1999-01-05 | Abbott Laboratories | Method for determination of item of interest in a sample |
| US5804141A (en) * | 1996-10-15 | 1998-09-08 | Chianese; David | Reagent strip slide treating apparatus |
| US7118878B1 (en) | 2000-06-09 | 2006-10-10 | Promega Corporation | Method for increasing luminescence assay sensitivity |
| CA2515217A1 (en) * | 2003-02-12 | 2004-08-26 | Promega Corporation | Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions |
| WO2008118445A1 (en) * | 2007-03-26 | 2008-10-02 | Promega Corporation | Methods to quench light from optical reactions |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3654090A (en) * | 1968-09-24 | 1972-04-04 | Organon | Method for the determination of antigens and antibodies |
| NL154599B (nl) * | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| AT357272B (de) * | 1976-09-18 | 1980-06-25 | Boehringer Mannheim Gmbh | Verfahren und vorrichtung zum nachweis und zur bestimmung eines spezifischen bindeproteins oder einer damit bindefaehigen substanz |
| FR2418463A1 (fr) * | 1978-02-28 | 1979-09-21 | Nal Transfusion Sanguine Centr | Procede pour le depistage ou l'identification, dans un milieu biologique, d'antigenes viraux, d'antigenes erythrocytaires ou cellulaires ou d'anticorps |
| US4222744A (en) * | 1978-09-27 | 1980-09-16 | Becton Dickinson & Company | Assay for ligands |
| US4299916A (en) * | 1979-12-26 | 1981-11-10 | Syva Company | Preferential signal production on a surface in immunoassays |
-
1980
- 1980-12-23 DE DE19803048884 patent/DE3048884A1/de not_active Withdrawn
-
1981
- 1981-10-22 DE DE8181108754T patent/DE3170532D1/de not_active Expired
- 1981-10-22 AT AT81108754T patent/ATE13356T1/de not_active IP Right Cessation
- 1981-10-22 EP EP81108754A patent/EP0054675B1/de not_active Expired
- 1981-12-02 CA CA000391389A patent/CA1170180A/en not_active Expired
- 1981-12-09 ES ES507817A patent/ES8302312A1/es not_active Expired
- 1981-12-14 DD DD81235744A patent/DD201943A5/xx not_active IP Right Cessation
- 1981-12-16 US US06/331,339 patent/US4743536A/en not_active Expired - Fee Related
- 1981-12-18 AU AU78668/81A patent/AU524904B2/en not_active Ceased
- 1981-12-22 JP JP56206303A patent/JPS57132060A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| US4743536A (en) | 1988-05-10 |
| EP0054675A1 (de) | 1982-06-30 |
| EP0054675B1 (de) | 1985-05-15 |
| AU7866881A (en) | 1982-07-22 |
| JPS57132060A (en) | 1982-08-16 |
| JPS6232422B2 (en, 2012) | 1987-07-14 |
| ES507817A0 (es) | 1983-01-16 |
| DE3170532D1 (en) | 1985-06-20 |
| CA1170180A (en) | 1984-07-03 |
| DD201943A5 (de) | 1983-08-17 |
| ATE13356T1 (de) | 1985-06-15 |
| ES8302312A1 (es) | 1983-01-16 |
| AU524904B2 (en) | 1982-10-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69516007T2 (de) | Verfahren zum nachweiss von antikörpern | |
| EP0363942B1 (de) | Verfahren zur Bestimmung einer spezifisch bindefähigen Substanz | |
| EP0397113B1 (de) | Verfahren zum Nachweis spezifisch bindefähiger Substanzen in Körperflüssigkeiten | |
| CH627281A5 (en, 2012) | ||
| DE3872500T2 (de) | Verwendung eines mit biotin verknuepften immobilisierten rezeptors in einer testvorrichtung, einem testsatz und einer methode zur bestimmung eines liganden. | |
| DE68918166T2 (de) | Spezifische Bindungszusammensetzung mit einem Protein oder einem Kohlenhydrat mit Nieder-pI und ein diagnostischer Testsatz und Verfahren zur Anwendung. | |
| EP0407904B1 (de) | Verfahren zur Bestimmung eines Analyten | |
| DE69114394T2 (de) | Analytisches Element mit biologisch aktivem Reagens und Verfahren zur Verwendung des Reagens. | |
| DE3303793C2 (de) | Immunoassay für klassenspezifische Immunoglobulinantikörper | |
| DE69025940T2 (de) | Durch Silber intensiviertes, auf Goldmarkierung beruhendes Immuntestverfahren | |
| DE2164768A1 (de) | Verfahren zum Nachweis und zur Bestimmung von spezifischen Bindeproteinen | |
| DE3905505A1 (de) | Polymere igg-derivate zur kompensation von stoerfaktoren in immunoassays | |
| EP0054675B1 (de) | Verfahren zur Enzymimmunobestimmung in heterogener Phase | |
| EP0356964B1 (de) | Verfahren zur Bestimmung einer spezifisch bindefähigen Substanz | |
| DE69106002T2 (de) | Testverfahren und reagenziensatz dafür. | |
| DE69012552T2 (de) | Avidin-biotin assistiertes immunassayverfahren. | |
| DE68917613T2 (de) | Natriumdecylsulfat enthaltende Waschlösung, Testsatz und Verfahren zur Bestimmung eines immunologischen Liganden. | |
| DE3433652A1 (de) | Immunchemisches messverfahren fuer haptene und proteine | |
| DE68916362T2 (de) | Immunologische Reagenz-Zusammensetzung und deren Verwendung zur Bestimmung von chlamydialen oder gonokokkalen Antigenen. | |
| DE68912315T2 (de) | Testverfahren für chlamydia unter verwendung einer basisbehandlung. | |
| DE69108439T2 (de) | Verfahren zur Bestimmung des Glykationsprozentsatzes eines bestimmten Proteins. | |
| DE69211805T2 (de) | Testsatz und Verfahren zum Nachweiss von Mikroorganismen assoziiert mit periodontalen Krankheiten unter Verwendung Surfactantmischung als Extraktionskomposition | |
| DE3688072T2 (de) | Immuntestverfahren und satz. | |
| EP0366092B1 (de) | Verfahren zur Bestimmung von für ein Antigen klassenspezifischen Antikörpern und dazu geeignetes Reagenz | |
| DE69013730T2 (de) | Für ganze Zellen geeigneter Satz und Verfahren zu enzymatischen Bestimmungen. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |