DE2967232D1 - Apparatus and process for continuous flow isoelectric focusing - Google Patents

Apparatus and process for continuous flow isoelectric focusing

Info

Publication number
DE2967232D1
DE2967232D1 DE7979900434T DE2967232T DE2967232D1 DE 2967232 D1 DE2967232 D1 DE 2967232D1 DE 7979900434 T DE7979900434 T DE 7979900434T DE 2967232 T DE2967232 T DE 2967232T DE 2967232 D1 DE2967232 D1 DE 2967232D1
Authority
DE
Germany
Prior art keywords
continuous flow
isoelectric focusing
flow isoelectric
focusing
continuous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE7979900434T
Other languages
English (en)
Inventor
Milan Bier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University Patents Inc
Original Assignee
University Patents Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Patents Inc filed Critical University Patents Inc
Application granted granted Critical
Publication of DE2967232D1 publication Critical patent/DE2967232D1/de
Expired legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44795Isoelectric focusing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D57/00Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
DE7979900434T 1978-04-18 1979-04-16 Apparatus and process for continuous flow isoelectric focusing Expired DE2967232D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US05/897,261 US4204929A (en) 1978-04-18 1978-04-18 Isoelectric focusing method
PCT/US1979/000238 WO1979000942A1 (en) 1978-04-18 1979-04-16 Apparatus and process for continuous flow isoelectric focusing

Publications (1)

Publication Number Publication Date
DE2967232D1 true DE2967232D1 (en) 1984-10-31

Family

ID=25407638

Family Applications (1)

Application Number Title Priority Date Filing Date
DE7979900434T Expired DE2967232D1 (en) 1978-04-18 1979-04-16 Apparatus and process for continuous flow isoelectric focusing

Country Status (5)

Country Link
US (1) US4204929A (de)
EP (1) EP0018375B1 (de)
JP (1) JPS55500261A (de)
DE (1) DE2967232D1 (de)
WO (1) WO1979000942A1 (de)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2902247C3 (de) * 1979-01-20 1981-12-24 Gkss - Forschungszentrum Geesthacht Gmbh, 2000 Hamburg Dichtungsrahmen für gestapelt angeordnete Austauschermembranen für die Elektrodialyse
US4322275A (en) * 1980-01-10 1982-03-30 Ionics Incorporated Fractionation of protein mixtures
US4351710A (en) * 1980-01-10 1982-09-28 Ionics, Incorporated Fractionation of protein mixtures
US4401538A (en) * 1981-06-02 1983-08-30 Hausfeld David A Isoelectric focusing techniques and devices
US4396477A (en) * 1981-06-29 1983-08-02 Ionics, Incorporated Separation of proteins using electrodialysis-isoelectric focusing combination
US4441978A (en) * 1981-06-29 1984-04-10 Ionics Incorporated Separation of proteins using electrodialysis - isoelectric focusing combination
US4533447A (en) * 1983-06-13 1985-08-06 Meldon Jerry H Apparatus for and method of isoelectric focussing
DE3337669C2 (de) * 1983-10-17 1989-09-21 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Gerät zur Elektroelution elektrisch geladener Makromoleküle
DE3337668C2 (de) * 1983-10-17 1985-12-05 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Verfahren zur Elektroelution elektrisch geladener Makromoleküle
US4588492A (en) * 1984-05-07 1986-05-13 University Patents, Inc. Rotating apparatus for isoelectric focusing
US4735697A (en) * 1985-04-17 1988-04-05 Phoresis Transfer Systems, Inc. Method and apparatus for separating complex mixtures of bio-organic materials
US4604174A (en) * 1985-04-30 1986-08-05 Dorr-Oliver Incorporated High flow electrofiltration
US5071536A (en) * 1985-11-25 1991-12-10 Ivory Cornelius F High resolution continuous flow electrophoresis
US4673483A (en) * 1986-03-20 1987-06-16 Ionics Incorporated Isoelectric focusing apparatus
US4897169A (en) * 1986-08-18 1990-01-30 Milan Bier Process and apparatus for recycling isoelectric focusing and isotachophoresis
US4997537A (en) * 1986-10-21 1991-03-05 Northeastern University High performance microcapillary gel electrophoresis
US5665216A (en) * 1986-10-21 1997-09-09 Northeastern University Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same
US4834862A (en) * 1988-09-12 1989-05-30 Duke University Ampholyte separation method and apparatus
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5160594A (en) * 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5080770A (en) * 1989-09-11 1992-01-14 Culkin Joseph B Apparatus and method for separating particles
US5336387A (en) * 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5173164A (en) * 1990-09-11 1992-12-22 Bioseparations, Inc. Multi-modality electrical separator apparatus and method
WO1992010588A1 (en) * 1990-12-06 1992-06-25 Affymax Technologies N.V. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
DE4116179A1 (de) * 1991-05-17 1992-11-19 Serva Feinbiochem Gmbh & Co Elektrophoresekammer
US5332484A (en) * 1993-08-02 1994-07-26 Hi-Tech Bio-Tech, Inc. Apparatus and process for the large scale separation and retrieval of proteins from cellular extracts
US6741344B1 (en) * 1994-02-10 2004-05-25 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US20030017081A1 (en) * 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5631734A (en) * 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US6090555A (en) * 1997-12-11 2000-07-18 Affymetrix, Inc. Scanned image alignment systems and methods
DE69527585T2 (de) * 1994-06-08 2003-04-03 Affymetrix Inc Verfahren und Vorrichtung zum Verpacken von Chips
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
US5662813A (en) * 1994-10-21 1997-09-02 Bioseparations, Inc. Method for separation of nucleated fetal erythrocytes from maternal blood samples
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US6720149B1 (en) * 1995-06-07 2004-04-13 Affymetrix, Inc. Methods for concurrently processing multiple biological chip assays
US5681392A (en) * 1995-12-21 1997-10-28 Xerox Corporation Fluid reservoir containing panels for reducing rate of fluid flow
WO1997043611A1 (en) * 1996-05-16 1997-11-20 Affymetrix, Inc. Systems and methods for detection of labeled materials
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
AUPP790698A0 (en) * 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US7612020B2 (en) * 1998-12-28 2009-11-03 Illumina, Inc. Composite arrays utilizing microspheres with a hybridization chamber
JP2003500645A (ja) * 1999-05-19 2003-01-07 ビラテック アクチェンゲゼルシャフト 荷電した分子を単離するための装置および方法
DE19931901A1 (de) * 1999-07-08 2001-01-11 Basf Ag Verfahren und Vorrichtung zum Parallel-Analysieren von kolloidalen Teilchen mit Feldflußfraktionierung
US7077942B1 (en) 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
ATE449200T1 (de) 2000-04-18 2009-12-15 Gradipore Ltd Trennung und behandlung von proben durch elektrophorese
AUPQ697300A0 (en) 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
DE10047088C2 (de) * 2000-09-21 2002-10-17 Gerhard Weber Medium für analytische und präparative Elektrophorese
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
JP2004510170A (ja) * 2000-10-06 2004-04-02 グラディポア リミテッド マルチポート型分離装置および方法
DE10063096C1 (de) * 2000-12-18 2002-09-12 Gerhard Weber Elektrophoresevorrichtung, Elektrophoreseverfahren unter Verwendung einer Elektrophoresevorrichtung und Verwendung der Elektrophoresevorrichtung
DE10063097B4 (de) * 2000-12-18 2007-04-19 Becton, Dickinson And Co. Elektrophoresevorrichtung
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
JP2003028835A (ja) * 2001-07-11 2003-01-29 Olympus Optical Co Ltd フリーフロー電気泳動素子、及びフリーフロー電気泳動法
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US7850835B2 (en) * 2003-05-09 2010-12-14 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US20040241659A1 (en) * 2003-05-30 2004-12-02 Applera Corporation Apparatus and method for hybridization and SPR detection
DE602004012894T2 (de) 2003-12-30 2009-05-14 Bioactive Polymers Ab Oberflächenschutz von frei liegenden biologischen geweben
SE0303588D0 (sv) 2003-12-30 2003-12-30 Bioactive Polymers Ab C O Lund Surface protection of exposed biological tissues
DE102004007848A1 (de) * 2004-02-17 2005-09-01 Bayer Technology Services Gmbh Vorrichtung und Verfahren für die Membranelektrophorese und Elektrofiltration
US20060246576A1 (en) 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
DE102005020134A1 (de) * 2005-04-29 2006-11-02 Becton, Dickinson And Co. Verfahren und Vorrichtung zur Durchführung eines parallelen und simultanen Mehrfachprozesses der trägerfreien isoelektrischen Fokussierung
DE102005044994A1 (de) * 2005-09-21 2007-04-05 Gesellschaft zur Förderung der Analytischen Wissenschaften e.V. Vorrichtung zur Trennung eines Analytgemisches und zur Detektion der Analytsubstanzen mittels elektrophoretischer Trennung
US9445025B2 (en) 2006-01-27 2016-09-13 Affymetrix, Inc. System, method, and product for imaging probe arrays with small feature sizes
US8055098B2 (en) * 2006-01-27 2011-11-08 Affymetrix, Inc. System, method, and product for imaging probe arrays with small feature sizes
US20080003667A1 (en) * 2006-05-19 2008-01-03 Affymetrix, Inc. Consumable elements for use with fluid processing and detection systems
EP2059799B1 (de) * 2006-08-29 2017-07-12 Becton Dickinson and Company Verfahren und vorrichtung zur trägerfreien ablenkungselektrophorese
US7572990B2 (en) * 2007-03-30 2009-08-11 Intermec Ip Corp. Keypad overlay membrane
EP2788746B1 (de) * 2011-11-22 2018-10-24 Stephen G. Haralampu Mikrofluidische vorrichtung mit gestopptem fluss sowie verfahren zur ladungsbasierten trennung komplexer analytmischungen

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2878178A (en) * 1955-04-15 1959-03-17 Bier Milan Continuous free-boundary flow electrophoresis
US3079318A (en) * 1959-01-15 1963-02-26 Bier Milan Electrical filtering process and device
US3677923A (en) * 1968-04-15 1972-07-18 Milan Bier Electroosmotic and electrodialysis concentration,purification,and desalting
NL7012366A (en) * 1970-08-20 1972-02-22 Electrical extraction technique - particularly for biological and pharmaceutical products
US3751356A (en) * 1970-12-22 1973-08-07 Daiichi Seiyaku Co Method and apparatus for electroextraction
US3870617A (en) * 1971-03-30 1975-03-11 Rhone Poulenc Sa Apparatus for forced flow electrophoresis
US3989613A (en) * 1973-05-16 1976-11-02 The Dow Chemical Company Continuous balanced flow fixed boundary electrophoresis
US3972791A (en) * 1974-05-06 1976-08-03 Harold Stern Fractionation of proteins by electrical means
SE394326B (sv) * 1975-10-21 1977-06-20 Aminkemi Ab Anordning for kylning vid elektrofores
US4040940A (en) * 1976-12-21 1977-08-09 The United States Of America As Represented By The Secretary Of The Department Of Health, Education And Welfare Electrophoretic fractional elution apparatus employing a rotational seal fraction collector

Also Published As

Publication number Publication date
EP0018375B1 (de) 1984-09-26
EP0018375A4 (de) 1982-07-12
EP0018375A1 (de) 1980-11-12
JPS55500261A (de) 1980-05-01
US4204929A (en) 1980-05-27
WO1979000942A1 (en) 1979-11-15

Similar Documents

Publication Publication Date Title
DE2967232D1 (en) Apparatus and process for continuous flow isoelectric focusing
GB2064114B (en) Continuous flow analysis method and apparatus
JPS54114546A (en) Coating method and apparatus therefor
JPS536282A (en) Spattering process and apparatus
JPS53148073A (en) Filtering method and apparatus therefor
DE3275206D1 (en) Process and apparatus for continuous steel-making
GB2033088B (en) Method and apparatus for making screw-threaded joints
GB2022168B (en) Pipeburying underwater-trenching apparatus and method
ZA795842B (en) Filtering method and apparatus therefor
GB2038220B (en) Soldering apparatus and method
DE3267580D1 (en) Process and apparatus for cooling bulky material
GB2019338B (en) Feed apparatus and method
IL57498A0 (en) Method and apparatus for jumigating
GB2023117B (en) Method and apparatus for the continuous conditioning of sludge
GB2037789B (en) Process and apparatus for demonomerizing polycapronamide
JPS54128986A (en) Liquid continuous ultraafiltration method and apparatus
JPS54110133A (en) Method and apparatus for continuous casting
PT75316A (en) Irrigation apparatus and method
HU185366B (en) Method and apparatus for membrane filtering
GB2030511B (en) Pupe making apparatus and method
GB2022483B (en) Method and apparatus for continuous extrusion
GB2033835B (en) Process and apparatus for welding plastics sections
JPS5494469A (en) Method and apparatus for making bellows
JPS54126248A (en) Method and apparatus for electrophoresis coating
JPS54119542A (en) Method and apparatus for coating

Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee