DE2735480C2 - Verfahren zur selektiven Inaktivierung der proteolytischen Enzymaktivität in einem bakteriellen α-Amylaseenzympräparat, daraus hergestelltes bakterielles α-Amylasepräparat und seine Verwendung zur Herstellung eines Stärkehydrolysats - Google Patents
Verfahren zur selektiven Inaktivierung der proteolytischen Enzymaktivität in einem bakteriellen α-Amylaseenzympräparat, daraus hergestelltes bakterielles α-Amylasepräparat und seine Verwendung zur Herstellung eines StärkehydrolysatsInfo
- Publication number
- DE2735480C2 DE2735480C2 DE2735480A DE2735480A DE2735480C2 DE 2735480 C2 DE2735480 C2 DE 2735480C2 DE 2735480 A DE2735480 A DE 2735480A DE 2735480 A DE2735480 A DE 2735480A DE 2735480 C2 DE2735480 C2 DE 2735480C2
- Authority
- DE
- Germany
- Prior art keywords
- amylase
- starch
- protease
- activity
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108091005804 Peptidases Proteins 0.000 title claims description 79
- 230000000694 effects Effects 0.000 title claims description 65
- 238000000034 method Methods 0.000 title claims description 41
- 238000002360 preparation method Methods 0.000 title claims description 40
- 102000035195 Peptidases Human genes 0.000 title claims description 14
- 230000001580 bacterial effect Effects 0.000 title claims description 7
- 229920002472 Starch Polymers 0.000 title description 90
- 235000019698 starch Nutrition 0.000 title description 89
- 239000008107 starch Substances 0.000 title description 80
- 229940024171 alpha-amylase Drugs 0.000 title description 36
- 102000004139 alpha-Amylases Human genes 0.000 title description 35
- 108090000637 alpha-Amylases Proteins 0.000 title description 35
- 238000004519 manufacturing process Methods 0.000 title description 8
- 230000000415 inactivating effect Effects 0.000 title 1
- 240000008042 Zea mays Species 0.000 claims description 45
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 45
- 239000004382 Amylase Substances 0.000 claims description 43
- 102000004190 Enzymes Human genes 0.000 claims description 40
- 108090000790 Enzymes Proteins 0.000 claims description 40
- 230000002779 inactivation Effects 0.000 claims description 37
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 36
- 235000005822 corn Nutrition 0.000 claims description 36
- 239000006188 syrup Substances 0.000 claims description 27
- 235000020357 syrup Nutrition 0.000 claims description 27
- 239000000203 mixture Substances 0.000 claims description 12
- 102000013142 Amylases Human genes 0.000 claims description 10
- 108010065511 Amylases Proteins 0.000 claims description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 230000003625 amylolytic effect Effects 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 65
- 239000004365 Protease Substances 0.000 description 63
- 235000019419 proteases Nutrition 0.000 description 61
- 229940088598 enzyme Drugs 0.000 description 40
- 239000011575 calcium Substances 0.000 description 36
- 235000018102 proteins Nutrition 0.000 description 36
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- 229910052791 calcium Inorganic materials 0.000 description 21
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 20
- 229930006000 Sucrose Natural products 0.000 description 18
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 18
- 230000008569 process Effects 0.000 description 18
- 239000002002 slurry Substances 0.000 description 18
- 239000005720 sucrose Substances 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 239000000243 solution Substances 0.000 description 15
- 238000001914 filtration Methods 0.000 description 14
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- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 11
- 229910001424 calcium ion Inorganic materials 0.000 description 11
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 11
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 9
- 235000019418 amylase Nutrition 0.000 description 9
- 235000009973 maize Nutrition 0.000 description 9
- 239000000047 product Substances 0.000 description 9
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- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 8
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- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 7
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 101000870232 Tenebrio molitor Diuretic hormone 1 Proteins 0.000 description 4
- 108090001015 cancer procoagulant Proteins 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 239000005018 casein Substances 0.000 description 4
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 4
- 235000021240 caseins Nutrition 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
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- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 229920002261 Corn starch Polymers 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 3
- 230000008033 biological extinction Effects 0.000 description 3
- 239000012496 blank sample Substances 0.000 description 3
- 159000000007 calcium salts Chemical class 0.000 description 3
- 239000008120 corn starch Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
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- 239000000835 fiber Substances 0.000 description 3
- 239000010200 folin Substances 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 230000002797 proteolythic effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical group OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
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- 235000013339 cereals Nutrition 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
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- 239000006228 supernatant Substances 0.000 description 2
- 229940100445 wheat starch Drugs 0.000 description 2
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- 101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 229920002245 Dextrose equivalent Polymers 0.000 description 1
- 101100346656 Drosophila melanogaster strat gene Proteins 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 108700040099 Xylose isomerases Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010019077 beta-Amylase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000012745 brilliant blue FCF Nutrition 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
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- 238000005119 centrifugation Methods 0.000 description 1
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- 235000003599 food sweetener Nutrition 0.000 description 1
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- 230000002538 fungal effect Effects 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 235000011868 grain product Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Emergency Medicine (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Jellies, Jams, And Syrups (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71210276A | 1976-08-05 | 1976-08-05 | |
| US05/712,103 US4235970A (en) | 1976-08-05 | 1976-08-05 | Protease inactivated α-amylase preparations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2735480A1 DE2735480A1 (de) | 1978-02-09 |
| DE2735480C2 true DE2735480C2 (de) | 1986-11-13 |
Family
ID=27108759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2735480A Expired DE2735480C2 (de) | 1976-08-05 | 1977-08-05 | Verfahren zur selektiven Inaktivierung der proteolytischen Enzymaktivität in einem bakteriellen α-Amylaseenzympräparat, daraus hergestelltes bakterielles α-Amylasepräparat und seine Verwendung zur Herstellung eines Stärkehydrolysats |
Country Status (13)
| Country | Link |
|---|---|
| JP (1) | JPS5344688A (Direct) |
| AR (1) | AR219710A1 (Direct) |
| AU (1) | AU515262B2 (Direct) |
| BR (1) | BR7705171A (Direct) |
| DE (1) | DE2735480C2 (Direct) |
| DK (1) | DK350177A (Direct) |
| ES (1) | ES461346A1 (Direct) |
| FR (1) | FR2368497A1 (Direct) |
| GB (1) | GB1546556A (Direct) |
| IT (1) | IT1085406B (Direct) |
| NL (1) | NL7708669A (Direct) |
| SE (1) | SE433090B (Direct) |
| TR (1) | TR19513A (Direct) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4299848A (en) * | 1979-06-08 | 1981-11-10 | International Telephone And Telegraph Corporation | Modified enzyme system to inhibit bread firming method for preparing same and use of same in bread and other bakery products |
| US4329429A (en) * | 1980-03-24 | 1982-05-11 | Pfizer Inc. | Lactase preparation |
| GB9703641D0 (en) * | 1997-02-21 | 1997-04-09 | Cerestar Holding Bv | Purified acid stable alpha-amylase from fungal origin |
| JP6119025B1 (ja) * | 2016-06-03 | 2017-04-26 | 啓雄 水井 | みりん類の製造方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1634310A (en) * | 1922-07-25 | 1927-07-05 | Jr Jokichi Takamine | Process of making alpha yeast stimulant |
| GB315877A (en) * | 1928-07-21 | 1930-12-18 | Albert Rene Boidin | Method of purification of solutions of enzymes for use in various industries and the like |
| US2683682A (en) * | 1950-02-06 | 1954-07-13 | Research Corp | Differential inactivation of enzymes |
| US3249512A (en) * | 1963-05-28 | 1966-05-03 | Harold E Bode | In situ dextrose production in crude amylaceous materials |
| US3524798A (en) * | 1967-05-23 | 1970-08-18 | Standard Brands Inc | Stabilization of alpha-amylase preparations |
| CA940070A (en) * | 1968-12-23 | 1974-01-15 | Jim S. Berry | Stabilized aqueous enzyme composition |
-
1977
- 1977-08-03 AU AU27578/77A patent/AU515262B2/en not_active Expired
- 1977-08-04 FR FR7724110A patent/FR2368497A1/fr active Granted
- 1977-08-04 NL NL7708669A patent/NL7708669A/xx not_active Application Discontinuation
- 1977-08-04 DK DK350177A patent/DK350177A/da not_active Application Discontinuation
- 1977-08-04 AR AR268685A patent/AR219710A1/es active
- 1977-08-04 ES ES461346A patent/ES461346A1/es not_active Expired
- 1977-08-04 BR BR7705171A patent/BR7705171A/pt unknown
- 1977-08-05 SE SE7708917A patent/SE433090B/xx not_active IP Right Cessation
- 1977-08-05 JP JP9343777A patent/JPS5344688A/ja active Granted
- 1977-08-05 IT IT26556/77A patent/IT1085406B/it active
- 1977-08-05 DE DE2735480A patent/DE2735480C2/de not_active Expired
- 1977-08-05 TR TR19513A patent/TR19513A/xx unknown
- 1977-08-05 GB GB32997/77A patent/GB1546556A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| TR19513A (tr) | 1979-03-29 |
| DK350177A (da) | 1978-02-06 |
| BR7705171A (pt) | 1978-04-25 |
| DE2735480A1 (de) | 1978-02-09 |
| JPS5344688A (en) | 1978-04-21 |
| AR219710A1 (es) | 1980-09-15 |
| IT1085406B (it) | 1985-05-28 |
| FR2368497B1 (Direct) | 1982-12-17 |
| FR2368497A1 (fr) | 1978-05-19 |
| JPS5734798B2 (Direct) | 1982-07-24 |
| SE7708917L (Direct) | 1978-02-06 |
| AU2757877A (en) | 1979-02-08 |
| GB1546556A (en) | 1979-05-23 |
| SE433090B (sv) | 1984-05-07 |
| ES461346A1 (es) | 1978-12-01 |
| AU515262B2 (en) | 1981-03-26 |
| NL7708669A (nl) | 1978-02-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8363 | Opposition against the patent | ||
| 8339 | Ceased/non-payment of the annual fee |