DE2344317C3 - Process for the production of high protein food and feed - Google Patents

Process for the production of high protein food and feed

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Publication number
DE2344317C3
DE2344317C3 DE2344317A DE2344317A DE2344317C3 DE 2344317 C3 DE2344317 C3 DE 2344317C3 DE 2344317 A DE2344317 A DE 2344317A DE 2344317 A DE2344317 A DE 2344317A DE 2344317 C3 DE2344317 C3 DE 2344317C3
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DE
Germany
Prior art keywords
protein
feed
production
thickened
high protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE2344317A
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German (de)
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DE2344317A1 (en
DE2344317B2 (en
Inventor
Hans Dr.-Ing. Maennedorf Mueller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemap AG
Original Assignee
Mueller Hans Dr Ing Maennedorf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CH1317772A external-priority patent/CH566108A5/en
Priority claimed from CH1505872A external-priority patent/CH572710A5/en
Priority claimed from CH538473A external-priority patent/CH572711A5/en
Application filed by Mueller Hans Dr Ing Maennedorf filed Critical Mueller Hans Dr Ing Maennedorf
Publication of DE2344317A1 publication Critical patent/DE2344317A1/en
Publication of DE2344317B2 publication Critical patent/DE2344317B2/en
Application granted granted Critical
Publication of DE2344317C3 publication Critical patent/DE2344317C3/en
Expired legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C21/00Whey; Whey preparations
    • A23C21/02Whey; Whey preparations containing, or treated with, microorganisms or enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/18Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/20Proteins from microorganisms or unicellular algae
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Sustainable Development (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)

Description

1515th

Die Erfindung betrifft ein Verfahren zur Herstellung hoch eiweißhaltiger Nahrungs- und Futtermittel durch Züchtung von Hefen in Molke, die außer Kohlehydraten auch gelöste Eiweißstoffe enthält, bei dem nach beendeter Züchtung das Substrat eingedickt und die eingedickte Masse getrocknet wird.The invention relates to a method for the production of foods and feeds with a high protein content Breeding of yeast in whey, which contains not only carbohydrates but also dissolved proteins, in which after When cultivation is complete, the substrate is thickened and the thickened mass is dried.

Es ist bekannt, Candida-Hefen, andere Hefen, z. B. Saccharomyces cerevisiae und andere Mikroorganismen in oxygenierten Kohlenwasserstoffen, welche sich von Erdölfraktionen mit 1 bis 30 Kohlenwasserstoffatomen im Molekül ableiten, zu züchten und dann aus dem gewonnenen Fermentationssubstrat die Proteinzellen von der überstehenden Flüssigkeit durch Zentrifugieren, Filtern (z. B. Membranfiltern [Mikroporenfiltration], Dialyse, umgekehrte Osmose), Ausflocken, Absetzen und Dekantieren abzutrennen und anschließend die so eingedickte Masse zu trocknen, z. B. im Sprüh-, Trori:mel-, Gefrier-, Vakuum-, Horden-, Ofen- od. dgl. Trockner (DE-OS 17 92 187). Je nach Ausgangssubstrat und Fermentationsführung lassen sich getrocknete Proteinzellen mit einem Eiweißgehalt bis zu über 80 Gew.-% (aus Äthanol) erzielen.It is known to use Candida yeasts, other yeasts e.g. B. Saccharomyces cerevisiae and other microorganisms in oxygenated hydrocarbons, which are derived from petroleum fractions with 1 to 30 hydrocarbon atoms in the molecule, and then from the fermentation substrate obtained, the protein cells from the supernatant liquid by centrifugation, filtering (e.g. membrane filters [micropore filtration ], Dialysis, reverse osmosis), flaking, settling and decanting and then drying the thickened mass, e.g. B. in the spray, T r ori: mel, freezer, vacuum, rack, oven od. Like. Dryer (DE-OS 17 92 187). Depending on the starting substrate and the fermentation process, dried protein cells with a protein content of up to 80% by weight (from ethanol) can be obtained.

Es ist auch schon bekannt. Hefen, z. B. Candida-Hefen. in Melasse zu züchten und anschließend durch Zentrifugieren von der Flüssigkeit zu trennen, worauf die eingedickte Masse getrocknet wird. Die Trockensubstanz wird dann als Futter- oder Nährhefe verwendet. Diese Hefe enthält je nach verwendetem Hefestamm 40 bis 50% Protein.It is already known. Yeasts e.g. B. Candida yeasts. to grow in molasses and then to separate from the liquid by centrifugation, whereupon the thickened mass is dried. The dry matter is then used as feed or nutritional yeast used. This yeast contains 40 to 50% protein, depending on the yeast strain used.

In ähnlicher Weise wird Molke aus Käsereien mit ca. 4 bis 5% Lactose als Nährsubstrat, z. B. Saccharomyces fragilis. auf Hefe verarbeitet, welche durch Zentrifugieren aus dem Nährsubstrat (Biomasse) nach zumindest weitgehendem Abbau der Milchsäure gewonnen wird (DEOS 20 01 743). Sowohl Melasse als auch Molke enthalten außer Kohlehydraten noch gelöste Eiweißstoffe wie Albumine und Globuline.In a similar way, whey from cheese dairies with approx. 4 to 5% lactose as a nutrient substrate, e.g. B. Saccharomyces fragilis. processed on yeast, which by centrifugation is obtained from the nutrient substrate (biomass) after at least extensive degradation of the lactic acid (DEOS 20 01 743). In addition to carbohydrates, both molasses and whey also contain dissolved proteins like albumins and globulins.

Hs ist bekannt, die Molke vor ihrer Verarbeitung einer Ultrafiltration zu unterwerfen. Diese Ultrafiliration hält -lic gelösten Eiweißstoffe zurück, die für sich getrennt aufgearbeitet werden. Das Filtrat enthält im wesentlichen nur noch die gelösten Kohlehydrate und Salze, und dieses Filtrat wird noch als Nährsubstrat für die Kultur der Hefen verwendet Die gewonnene »Biomasse«, d. h. das aus Hefen bestehende Eiweiß, wird abzentrifugiert, gewaschen und getrocknet Obwohl bei diesem Verfahren eine Aufarbeitung der gelösten Eiweißstoffe möglich ist und damit die beim üblichen Verfahren, der Abtrennung dor Biomasse durch Separatoren, lästige Verunreinigung des Abwassers mit gelösten Eiweißstoffen vermieden werden kann, ist es wünschenswert, die aufwendige Verarbeitung durch Separatoren zu vermeiden. Hs is known to take the whey before it is processed Subject to ultrafiltration. This ultrafiltration holds back -lic dissolved protein substances, which are separated for themselves be worked up. The filtrate essentially only contains the dissolved carbohydrates and salts, and this filtrate is still used as a nutrient substrate for the yeast culture. H. the yeast-based protein is centrifuged off, washed and dried, although with this one Process a work-up of the dissolved proteins is possible and thus the usual process, the Separation of the biomass by separators, annoying contamination of the waste water with dissolved proteins can be avoided, it is desirable to avoid the complex processing by separators.

Die Aufgabe der Erfindung wird darin gesehen, bei der Herstellung hoch eiweißhaltiger Nahrungs- und Futtermittel durch Züchtung von Hefen die Trennung mit Separatoren oder Zentrifugen zu vermeiden und gegenüber dem normalen Eiweißgehalt von 45 — 50% einen höheren Eiweißgehalt des Produktes zu erreichen.The object of the invention is seen in the production of high protein food and Feed by breeding yeasts to avoid separation with separators or centrifuges and To achieve a higher protein content of the product compared to the normal protein content of 45 - 50%.

Die Lösung dieser Aufgabe besteht für das eingangs genannte Verfahren darin, daß nach beende.er Züchtung aus dem Substrat die Hefen zusammen mit dem gelösten Eiweiß durch Ultrafiltration abgetrennt werden und die derart eingedickte Masse getrocknet wird. Bei dem erfindungsgemäßen Verfahren wird die Ultrafiltration nach der Züchtung eingesetzt, denn es hat sich überraschenderweise gezeigt daß die Hefe bei der Ultrafiltration nicht verstopfend, sondern reinigend auf die Membranen der Ultrafilter wirkt Das Verfahren erlaubt einen höheren Eiweißgehalt als bisher im Produkt zu erzielen, da es möglich ist, das gelöste Eiweiß zusammen mit der Biomasse zu gewinnen, was besonders vorteilhaft bei Verwendung von Saccharomyces fragilis als Hefe der Fall istThe solution to this problem is for the method mentioned at the outset that after breeding the yeast and the dissolved protein are separated from the substrate by ultrafiltration and the thus thickened mass is dried. In the method according to the invention, the Ultrafiltration used after the cultivation, because it has surprisingly been shown that the yeast at ultrafiltration does not clog, but cleans the membranes of the ultrafilter. The process allows a higher protein content to be achieved in the product than before, as it is possible to use the dissolved To gain protein together with the biomass, which is particularly advantageous when using Saccharomyces fragilis is the case as yeast

Vielfach ist es zweckmäßig, wenn die Ultrafiltration in zwei Stufen erfolgt, nämlich in einer ersten Eindickurig und in einer Auswaschung mit anschließender zweiter Eindickung, um Salzreste oder dergleichen kostensparend auswaschen zu können. Die Eiweißlösung kann vor oder nach der Ultrafiltration chemisch gebleicht werden.It is often useful if the ultrafiltration takes place in two stages, namely in a first thickening process and in a washout followed by a second thickening to remove salt residues or the like in a cost-saving manner being able to wash out. The protein solution can be chemically bleached before or after the ultrafiltration will.

Beispielexample

Molke aus Käsereien mit einem Lactosegehalt von ca. 4% und einem Gehalt an gelöstem Eiweiß von ca 0,7% wird mit Saccharomyces fragilis geimpft. Dieser Organismus vermehrt sich in diesem Nährsubstrat und wandelt den Kohlehydratgehalt in Eiweißstoffe um. Anschließend an die Züchtung wird die gesamte Masse ultrafiltriert und so auf ca. I : 20 eingedickt. Durch Zusatz von Wasser und nochmaliger Ultrafiltration können Salzreste ausgewaschen werden. Das Konzentrat wird nun getrocknet, wobei sich Trommeltrockner oder Zerstäubungstrockner eignen. Das Trockenprodukt enthält ca. 55 bis 60% Eiweiß. Da es außer der Zellsubstanz der Mikroorganismen auch die Eiweißkörper enthält, die im Nährsubstrat gelöst vorhanden waren, hat es einen bedeutend höheren Proteingehalt und ist demzufolge wertvoller. Außerdem hat das Verfahren den Vorteil, daß eine Separierungsstufe mit Zentrifugen überflüssig wird.Whey from cheese factories with a lactose content of approx. 4% and a dissolved protein content of approx. 0.7% is vaccinated with Saccharomyces fragilis. This organism multiplies in this nutrient substrate and converts the carbohydrate content into protein. Subsequent to breeding is the entire mass ultrafiltered and so thickened to about 1:20. By adding water and repeated ultrafiltration salt residues can be washed out. The concentrate is now dried, using a drum dryer or spray dryers. The dry product contains approx. 55 to 60% protein. Since it is except the Cell substance of the microorganisms also contains the protein bodies, which are present dissolved in the nutrient substrate it has a significantly higher protein content and is therefore more valuable. Besides, that has Method has the advantage that a separation stage with centrifuges is superfluous.

Claims (1)

Patentanspruch:Claim: Verfahren zur Herstellung hoch eiweißhaltiger Nahrungs- und Futtermittel durch Züchtung von Hefen in Molke, die außer Kohlehydraten auch gelöste Eiweißstoffe enthält, bei dem nach beendeter Züchtung das Substrat eingedickt und die eingedickte Masse getrocknet wird, dadurch gekennzeichnet, daß nach beendeter Züchtung aus dem Substrat die Hefen zusammen mit dem gelösten Eiweiß durch Ultrafiltration abgetrennt werden und die derartig eingedickte Masse getrocknet wird.Process for the production of high protein food and feed by breeding Yeast in whey, which in addition to carbohydrates also contains dissolved proteins, in which after Cultivation the substrate is thickened and the thickened mass is dried, characterized in that that after the end of cultivation from the substrate the yeasts together with the dissolved Egg whites are separated by ultrafiltration and the mass thickened in this way is dried.
DE2344317A 1972-09-06 1973-09-03 Process for the production of high protein food and feed Expired DE2344317C3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH1317772A CH566108A5 (en) 1972-09-06 1972-09-06 High protein food- and feedstuffs prodn - by culturing micro organisms in protein-contg medium and ultrafiltration
CH1505872A CH572710A5 (en) 1972-10-13 1972-10-13 High protein food- and feedstuffs prodn - by culturing micro organisms in protein-contg medium and ultrafiltration
CH538473A CH572711A5 (en) 1973-04-16 1973-04-16 High protein food- and feedstuffs prodn - by culturing micro organisms in protein-contg medium and ultrafiltration

Publications (3)

Publication Number Publication Date
DE2344317A1 DE2344317A1 (en) 1974-04-25
DE2344317B2 DE2344317B2 (en) 1978-08-03
DE2344317C3 true DE2344317C3 (en) 1983-03-03

Family

ID=27175184

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2344317A Expired DE2344317C3 (en) 1972-09-06 1973-09-03 Process for the production of high protein food and feed

Country Status (6)

Country Link
JP (1) JPS5522079B2 (en)
CA (1) CA992015A (en)
DE (1) DE2344317C3 (en)
FR (1) FR2197524B1 (en)
GB (1) GB1414529A (en)
IT (1) IT1048139B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51121589A (en) * 1975-04-14 1976-10-23 Sanraku Inc Process for separating and producing microbial cells
CH621925A5 (en) * 1975-07-30 1981-03-13 Mueller Hans Dr Ing Fa Process for isolating proteins from whey and protein- and starch-containing natural products
FR2497635A1 (en) * 1981-01-14 1982-07-16 Bioprox Produits Biochimiques PROCESS FOR THE PREPARATION OF A FOOD FLAVOR WITH VEGETABLE TASTE AND A FOOD FLAVOR THUS OBTAINED

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1556384A (en) * 1967-12-29 1969-02-07
FR2049425A5 (en) * 1969-06-10 1971-03-26 Fermentation Whey fermented with yeast to give high - protein feedstuff
CA990571A (en) * 1971-03-19 1976-06-08 Kansas State University Research Foundation Process for conversion of whey solids to an edible yeast cell mass

Also Published As

Publication number Publication date
IT1048139B (en) 1980-11-20
FR2197524B1 (en) 1977-09-16
CA992015A (en) 1976-06-29
GB1414529A (en) 1975-11-19
FR2197524A1 (en) 1974-03-29
DE2344317A1 (en) 1974-04-25
JPS5522079B2 (en) 1980-06-14
JPS4966879A (en) 1974-06-28
DE2344317B2 (en) 1978-08-03

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Date Code Title Description
BF Willingness to grant licences
C3 Grant after two publication steps (3rd publication)
8327 Change in the person/name/address of the patent owner

Owner name: CHEMAP AG, 8708 MAENNEDORF, ZUERICH, CH

8381 Inventor (new situation)

Free format text: MUELLER, HANS, DR.-ING., 8708 MAENNEDORF, ZUERICH, CH

8339 Ceased/non-payment of the annual fee