FR2497635A1 - PROCESS FOR THE PREPARATION OF A FOOD FLAVOR WITH VEGETABLE TASTE AND A FOOD FLAVOR THUS OBTAINED - Google Patents

PROCESS FOR THE PREPARATION OF A FOOD FLAVOR WITH VEGETABLE TASTE AND A FOOD FLAVOR THUS OBTAINED Download PDF

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Publication number
FR2497635A1
FR2497635A1 FR8100921A FR8100921A FR2497635A1 FR 2497635 A1 FR2497635 A1 FR 2497635A1 FR 8100921 A FR8100921 A FR 8100921A FR 8100921 A FR8100921 A FR 8100921A FR 2497635 A1 FR2497635 A1 FR 2497635A1
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Prior art keywords
yeasts
whey
lysing
process according
veal
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FR8100921A
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FR2497635B1 (en
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Gilbert Ritzenthaler
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BIOPROX PRODUITS BIOCHIMIQUES
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BIOPROX PRODUITS BIOCHIMIQUES
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Priority to FR8100921A priority Critical patent/FR2497635A1/en
Priority to PCT/FR1982/000006 priority patent/WO1982002323A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/26Meat flavours
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14Yeasts or derivatives thereof
    • A23L33/145Extracts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/063Lysis of microorganisms of yeast

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Seeds, Soups, And Other Foods (AREA)
  • Seasonings (AREA)

Abstract

This method consists in lysing yeasts of the Kluyveromyces fragil is type grown on a medium comprised of lactoserum, protein-free lactoserum, lactoserum permeate or by-products of the same type. Product or flavoured culinary speciality with the product obtained by implementation of this method.

Description

La présente invention concerne un procédé de préparation d'un arôme alimentaire à goût de viande de veau et l'arôme alimentaire ainsi obtenu. The present invention relates to a process for preparing a food flavoring flavored with veal and the food flavoring thus obtained.

On trouve dans la littérature les descriptions de nombreux produits donnant des goûts de viande de boeuf, de porc, de volaille mais très peu de produits ont été décrits comme donnant un goût de viande dc veau. We find in the literature descriptions of many products giving tastes of beef, pork, poultry but very few products have been described as giving a taste of veal.

La présente invention s'est donnée pour but de préparer un arôme capable de communiquer un goût de viande de veau à une préparation alimentaire. The object of the present invention is to prepare a flavor capable of imparting a taste of veal to a food preparation.

La demanderesse a découvert, au cours de ses recherches, qu'il était possible d'obtenir par autolyse de levures de type Kluseromyces fragilis cultivées sur un milieu constitué de lactosérum, de lactosérum déprotéiné , de permet de lactosérum ou de sous-produits de fromagerie de même type, un autolysat présentant un excellent goût de viande de veau grillée. The Applicant has discovered, during its research, that it was possible to obtain by autolysis yeasts of the Kluseromyces fragilis type cultivated on a medium consisting of whey, deproteinized whey, whey allows or cheese by-products. of the same type, an autolysate with an excellent taste of grilled veal.

Ce produit présente donc un intcrêt pour renforcer le goût de préparations à base de viande de veau, pour l'aromatisation de sauces,et/aide préparations à base de protéines texturées. This product is therefore of interest to reinforce the taste of preparations based on veal, for flavoring sauces, and / help preparations based on textured proteins.

Le procédé d'obtention de cet arôme consiste à ajouter à de la crème de levures fraiches séparées de leur milieu de culture et lavées, un sel alimentaire etXou un agent plasmolysant organique tel que l'acétate d'éthyle, d'ajuster le pH de la suspension entre 3,0 et 8,0, et de préférence entre 4 et 6,5, puis de chauffer celle-ci entre 30 et 650 et de préférence aux environs de 500C, puis de maintenir cette température pendant 12 a 72 heures.La plasmolyse peut aussi être réaliséc par addition d'enzymes à action lysante sur les membranes de la levure telles que des enzymes lysant issues de Bacillus Cirulans, Aspergillus luteus, Lysozyme ou tout autre enzyme ayant pour fonction d'altérer et d'hydrolyser la membrane cellulaire des levures pour en libérer le contenu cellulaire. The process for obtaining this flavor consists in adding to a cream of fresh yeasts separated from their culture medium and washed, a food salt and or an organic plasmolysing agent such as ethyl acetate, to adjust the pH of the suspension between 3.0 and 8.0, and preferably between 4 and 6.5, then heat the latter between 30 and 650 and preferably around 500C, then maintain this temperature for 12 to 72 hours. Plasmolysis can also be carried out by adding enzymes with a lysing action on the yeast membranes such as lysing enzymes from Bacillus Cirulans, Aspergillus luteus, Lysozyme or any other enzyme which has the function of altering and hydrolyzing the membrane. yeast cells to release the cell contents.

L'autolyse peut être rendue plus complète par addition d'une protéase d'origine animale, végétale, fongique ou bactérienne.  Autolysis can be made more complete by adding a protease of animal, vegetable, fungal or bacterial origin.

La suspension obtenue peut ensuite être filtrée ou centrifugé pour éliminer les résidus cellulaires inso lubies, la partie solubilisée étant concentrée à l'état pâteux ou séchée. The suspension obtained can then be filtered or centrifuged to remove insoluble cellular residues, the solubilized part being concentrated in the pasty state or dried.

Si le produit est destiné a être employé dans des préparations non limpides ou dans des produit pour lesquels les résidus cellulaires n'apportent pas de gêne, la suspension peut etre séchée telle que sans séparation des insolubles, car ces derniers ne présentent pas de goût désagréable comme c'est le cas pour les levures de boulangerie par exemple. If the product is intended to be used in non-clear preparations or in products for which the cellular residues do not cause discomfort, the suspension can be dried as without separation of the insolubles, since the latter do not present an unpleasant taste as is the case for baker's yeast for example.

Exemple i
i kg de crème de levures lavées, du type Kluyvero myces fragilis, à 10 % de matières sèches, est additionné de 20 g de chlorure de sodium et de 50 g d'acé-tate d'é- thyle.
Example i
20 g of sodium yeast cream, of the Kluyvero myces fragilis type, containing 10% dry matter, is added with 20 g of sodium chloride and 50 g of ethyl acetate.

Le pH de la suspension est ajuste a 5,8 avec de la soude, puis le produit est porté a -;O0C et maintenu à cette température pendant 48 heures. Après autolyse, le produit est filtré et concentré a 80 % de matières sèches. Ce produit possède un goût de viande de veau grille très agréable. The pH of the suspension is adjusted to 5.8 with sodium hydroxide, then the product is brought to -; 0OC and maintained at this temperature for 48 hours. After autolysis, the product is filtered and concentrated to 80% dry matter. This product has a very pleasant grilled veal flavor.

Exemple 2
i kg de crème de levares lavées, du type Kluyvero- myces fragilis, a 10 % de matières sèches, est additionné de 20 g de chlorure de sodium et de 10 g d'acétate d'éthyle.
Example 2
20 kg of sodium chloride and 10 g of ethyl acetate are added to 1 kg of washed yeast cream, of the Kluyveromies fragilis type, at 10% dry matter.

Le pH de la suspension est ajusté a 5,5 puis on ajoute 70 mg d'une protéase commerciale. The pH of the suspension is adjusted to 5.5 and then 70 mg of a commercial protease are added.

La supension est chauffée â 480C et maintenue à cette température pendant 24 heures. Le pH est ensuite ajusté à 7 - 7,5 avec une solution concentrée de soude et la température est ajustée à 50 C. Cette température est encore maintenue pendant 24 heures. The suspension is heated to 480C and kept at this temperature for 24 hours. The pH is then adjusted to 7 - 7.5 with a concentrated sodium hydroxide solution and the temperature is adjusted to 50 C. This temperature is maintained for 24 hours.

La suspension est ensuite concentrée puis séchée sous vide. On obtient ainsi une poudre avec un goût de viande de veau grillee. The suspension is then concentrated and then dried under vacuum. A powder is thus obtained with a taste of grilled veal.

Exemple 3
1 kg de crème le levures lavées Kluyveromyces fragilis à 10 % de matières sèches est additionné de 100 mg d'une préparation enzymatique extraite de A-luteus et de 100 mg de Lyzozyme purifié. Le pH du mélange est ajusté à 7,5 et la température est maintenue à 370C pendant 2 heures.
Example 3
1 kg of cream the washed yeast Kluyveromyces fragilis with 10% of dry matter is added with 100 mg of an enzymatic preparation extracted from A-luteus and with 100 mg of purified Lyzozyme. The pH of the mixture is adjusted to 7.5 and the temperature is maintained at 370C for 2 hours.

La lyse des cellules étant alors réalisée, le pH de la suspension est ajusté a 5,5 et la température est portée à 480C. Cette température est maintenue pendant 48 heures pour réaliser l'autolyse. The lysis of the cells being then carried out, the pH of the suspension is adjusted to 5.5 and the temperature is brought to 480C. This temperature is maintained for 48 hours to perform autolysis.

Après autolyse, le produit est filtré et concentré à 80 X de matières sèches. After autolysis, the product is filtered and concentrated to 80% dry matter.

Le produit obtenu possède un très bon goût de viande de veau, comme les produits des exemples 1 et 2. The product obtained has a very good taste of veal, like the products of Examples 1 and 2.

Exemple 4
On prépare un fond de sauce deshydratée pour blanquette de veau selon la formule suivante
Arôme de veau deshydraté préparé selon
l'exemple 2 : 15
glutamate monosodique 5
gras de boeuf 8,5
lait en poudre 23,7
sel 5
sucre 0,8
farine de blé 20
amidon de mais 20
oignons frais en poudre 0,3
gomme xanthane 1,5
divers épices et condiments 0,2
100,0
On fait parallèlement un fond de sauce en rempla çant l'arôme de veau par un extrait de levure en poudre, les autres éléments restant les mêmes.
Example 4
We prepare a base of dehydrated sauce for veal stew according to the following formula
Dehydrated veal flavor prepared according to
example 2: 15
monosodium glutamate 5
beef fat 8.5
milk powder 23.7
salt 5
0.8 sugar
wheat flour 20
corn starch 20
fresh onion powder 0.3
xanthan gum 1.5
various spices and condiments 0.2
100.0
A sauce base is made at the same time by replacing the veal flavor with a powdered yeast extract, the other elements remaining the same.

On fait une sauce en dispersant une partie de sauce déshydratée dans 10 parties d'eau, en portant à ltébullition et en maintenant à l'ébullition légère pendant 5 minutes. A sauce is made by dispersing one part of the dehydrated sauce in 10 parts of water, bringing to the boil and maintaining the light boiling for 5 minutes.

Une dégustation comptée des deux sauces montre que celle qui est préparée avec l'arôme de veau selon l'exemple 2 semble réellement contenir de la glace de veau alors que l'autre sauce est une sauce blanche sans caractère particulier. A counted tasting of the two sauces shows that the one prepared with the veal flavor according to example 2 really seems to contain veal ice cream while the other sauce is a white sauce with no particular character.

La sauce obtenue avec l'arôme de veau permet donc d'accompagner une blanquette ou une garniture de bouchées à la reine contenant du ris de veau et des protéines végétales texturées ou toute autre préparation culinaire à base de veau ou à laquelle on souhaite communiquer un goût de viande de veau.  The sauce obtained with the aroma of veal therefore makes it possible to accompany a blanquette or a garnish of queen-size bites containing sweetbread and textured vegetable proteins or any other culinary preparation based on veal or to which one wishes to communicate a taste of veal.

Claims (6)

- REVENDICATIONS  - CLAIMS 1 - Procédé de préparation d'un arôme a goût de viande de veau, caractérisé en ce qu'il consiste à lyser des levures du type Kluyveromyces fragilis cultivées sur un milieu constitué de lactosérum, de lactosérum déprotéiné, de permeat de lactosérum ou de sous-produits du même type. 1 - Process for the preparation of a flavor of veal meat, characterized in that it consists in lysing yeasts of the Kluyveromyces fragilis type cultivated on a medium consisting of whey, deproteinized whey, whey permeat or sub -products of the same type. 2 - Procédé selon la revendication 1, caractérisé en ce que la lyse des levures est effectuée par plasmolyse suivie d'autolyse à l'aide de sels alimentaires et/ou d'agents plasmolysants organiques par chauffage à une tempe' rature située entre 30 et 650C et de préférence aux environs de 500C, à un pH compris entre 3 et 8 et se situant de préférence entre 4 et 6,5 pendant une durée suffisante à l'obtention de l'effet recherché et de préférence entre 12 et 72 heures. 2 - Process according to claim 1, characterized in that the lysis of the yeasts is carried out by plasmolysis followed by autolysis using food salts and / or organic plasmolysing agents by heating to a temperature between 30 and 650C and preferably around 500C, at a pH between 3 and 8 and preferably between 4 and 6.5 for a time sufficient to obtain the desired effect and preferably between 12 and 72 hours. 3 - Procédé selon la revendication 2, caractérisé en ce qu'on procède en début d'opération à l'addition de protéases d'origine animale, végétale, fongique ou microbienne pour réaliser ou achever l'hydrolyse. 3 - Method according to claim 2, characterized in that one proceeds at the start of the operation to the addition of proteases of animal, vegetable, fungal or microbial origin to achieve or complete the hydrolysis. 4 - Procédé selon la revendication 1, caractérisé en ce que la plasmolyse et l'autolyse sont réalisées par addition d'enzymes à action lysante sur les membranes de la levure telles que des enzymes lysantes issues due Bacillus 4 - Process according to claim 1, characterized in that the plasmolysis and autolysis are carried out by adding enzymes with a lysing action on the membranes of the yeast such as lysing enzymes derived from Bacillus Cirulans, Aspergillus luteus, Lysozyme ou tout autre enzyme susceptible d'altérer et d'hydrolyser la membrane cellulaire des levures pour en libérer le contenu cellulaire.Cirulans, Aspergillus luteus, Lysozyme or any other enzyme capable of altering and hydrolyzing the cell membrane of yeasts to release the cell contents. 5 - Procédé selon l'une quelconque des revendications 1 à 4, caractérisé en ce que l'extrait soluble est séparé par filtration ou centrifugation puis concentré et/ou séché. 5 - Process according to any one of claims 1 to 4, characterized in that the soluble extract is separated by filtration or centrifugation then concentrated and / or dried. 6 - Produit ou spécialité culinaire aromatisé avec le produit obtenu par mise en oeuvre du procédé selon l'une quelconque des revendications 1 à 5.  6 - Product or culinary specialty flavored with the product obtained by implementing the method according to any one of claims 1 to 5.
FR8100921A 1981-01-14 1981-01-14 PROCESS FOR THE PREPARATION OF A FOOD FLAVOR WITH VEGETABLE TASTE AND A FOOD FLAVOR THUS OBTAINED Granted FR2497635A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR8100921A FR2497635A1 (en) 1981-01-14 1981-01-14 PROCESS FOR THE PREPARATION OF A FOOD FLAVOR WITH VEGETABLE TASTE AND A FOOD FLAVOR THUS OBTAINED
PCT/FR1982/000006 WO1982002323A1 (en) 1981-01-14 1982-01-13 Preparation of a meat flavouring

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Application Number Priority Date Filing Date Title
FR8100921A FR2497635A1 (en) 1981-01-14 1981-01-14 PROCESS FOR THE PREPARATION OF A FOOD FLAVOR WITH VEGETABLE TASTE AND A FOOD FLAVOR THUS OBTAINED

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FR2497635A1 true FR2497635A1 (en) 1982-07-16
FR2497635B1 FR2497635B1 (en) 1983-03-25

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007003773A2 (en) * 2005-07-01 2007-01-11 Societe D'extraction Des Principes Actifs Sa (Vincience) Use of a yeast extract in the form of an active agent for inducing a synthesis of sirt proteins in skin cells
WO2009007424A1 (en) * 2007-07-10 2009-01-15 Dsm Ip Assets B.V. Yeast autolysates

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3661676D1 (en) * 1985-01-31 1989-02-16 Unilever Nv Food flavours
US5486368A (en) * 1992-05-28 1996-01-23 Dmv Usa, Inc. Production of a cultured yeast product from whey permeate, yeast cream and yeast centrate
WO2012011402A1 (en) * 2010-07-22 2012-01-26 キッコーマン株式会社 Yeast extract containing lactic acid

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE112099C (en) *
US3394013A (en) * 1964-11-02 1968-07-23 Procter & Gamble Flavored fatty material and process for preparing the same
FR2033934A5 (en) * 1969-02-08 1970-12-04 Kyowa Hakko Kogyo Kk
FR2197524A1 (en) * 1972-09-06 1974-03-29 Mueller Hans
US3809780A (en) * 1969-02-08 1974-05-07 Kyowa Hakko Kogyo Kk Preparation of a seasoning agent

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE112099C (en) *
US3394013A (en) * 1964-11-02 1968-07-23 Procter & Gamble Flavored fatty material and process for preparing the same
FR2033934A5 (en) * 1969-02-08 1970-12-04 Kyowa Hakko Kogyo Kk
US3809780A (en) * 1969-02-08 1974-05-07 Kyowa Hakko Kogyo Kk Preparation of a seasoning agent
FR2197524A1 (en) * 1972-09-06 1974-03-29 Mueller Hans

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EXBK/77 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007003773A2 (en) * 2005-07-01 2007-01-11 Societe D'extraction Des Principes Actifs Sa (Vincience) Use of a yeast extract in the form of an active agent for inducing a synthesis of sirt proteins in skin cells
WO2007003773A3 (en) * 2005-07-01 2007-04-05 Soc Extraction Principes Actif Use of a yeast extract in the form of an active agent for inducing a synthesis of sirt proteins in skin cells
WO2009007424A1 (en) * 2007-07-10 2009-01-15 Dsm Ip Assets B.V. Yeast autolysates
JP2010532981A (en) * 2007-07-10 2010-10-21 ディーエスエム アイピー アセッツ ビー.ブイ. Yeast autolysate
AU2008274259B2 (en) * 2007-07-10 2013-09-12 Dsm Ip Assets B.V. Yeast autolysates
EA018646B1 (en) * 2007-07-10 2013-09-30 ДСМ АйПи АССЕТС Б.В. Yeast autolysate, process for preparing and use thereof, flavor aromatic improver in food applications based thereon
CN101686720B (en) * 2007-07-10 2016-04-20 帝斯曼知识产权资产管理有限公司 Yeast autolyzate

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WO1982002323A1 (en) 1982-07-22
FR2497635B1 (en) 1983-03-25

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