DE2339663A1 - Testloesung zum nachweis der aktivitaet von succinodehydrogenase - Google Patents
Testloesung zum nachweis der aktivitaet von succinodehydrogenaseInfo
- Publication number
- DE2339663A1 DE2339663A1 DE19732339663 DE2339663A DE2339663A1 DE 2339663 A1 DE2339663 A1 DE 2339663A1 DE 19732339663 DE19732339663 DE 19732339663 DE 2339663 A DE2339663 A DE 2339663A DE 2339663 A1 DE2339663 A1 DE 2339663A1
- Authority
- DE
- Germany
- Prior art keywords
- methylene blue
- powdery mixture
- preparation according
- sodium succinate
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000694 effects Effects 0.000 title description 3
- 239000012085 test solution Substances 0.000 title description 2
- 239000000203 mixture Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 10
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 229940074404 sodium succinate Drugs 0.000 claims description 8
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 claims description 8
- 239000008363 phosphate buffer Substances 0.000 claims description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 claims description 4
- 206010028980 Neoplasm Diseases 0.000 claims description 4
- 201000011510 cancer Diseases 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000001488 sodium phosphate Substances 0.000 claims description 4
- 229960005070 ascorbic acid Drugs 0.000 claims description 3
- 235000010323 ascorbic acid Nutrition 0.000 claims description 3
- 239000011668 ascorbic acid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 235000019800 disodium phosphate Nutrition 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 2
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims 4
- GAYQZIFREDQBAB-UHFFFAOYSA-N 1-phenyl-2h-tetrazol-1-ium;chloride Chemical compound [Cl-].C1=NN=N[NH+]1C1=CC=CC=C1 GAYQZIFREDQBAB-UHFFFAOYSA-N 0.000 claims 1
- 230000014155 detection of activity Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 9
- 238000011534 incubation Methods 0.000 description 5
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 5
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- ISOVWEWRUVBJME-UHFFFAOYSA-N 1,2,3-triphenyltetrazole Chemical class C1=NN(C=2C=CC=CC=2)N(C=2C=CC=CC=2)N1C1=CC=CC=C1 ISOVWEWRUVBJME-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000001143 conditioned effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 241000272201 Columbiformes Species 0.000 description 1
- 206010063045 Effusion Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010034960 Photophobia Diseases 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- -1 hydrogen ions Chemical class 0.000 description 1
- 208000013469 light sensitivity Diseases 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 210000004910 pleural fluid Anatomy 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7228444A FR2196082A5 (https=) | 1972-08-07 | 1972-08-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2339663A1 true DE2339663A1 (de) | 1974-02-21 |
Family
ID=9102971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19732339663 Pending DE2339663A1 (de) | 1972-08-07 | 1973-08-04 | Testloesung zum nachweis der aktivitaet von succinodehydrogenase |
Country Status (3)
| Country | Link |
|---|---|
| DE (1) | DE2339663A1 (https=) |
| FR (1) | FR2196082A5 (https=) |
| GB (1) | GB1401896A (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1687286A1 (en) * | 2003-11-28 | 2006-08-09 | Photopharmica Limited | Developments in biologically active methylene blue derivatives (2) |
-
1972
- 1972-08-07 FR FR7228444A patent/FR2196082A5/fr not_active Expired
-
1973
- 1973-07-02 GB GB3132173A patent/GB1401896A/en not_active Expired
- 1973-08-04 DE DE19732339663 patent/DE2339663A1/de active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FR2196082A5 (https=) | 1974-03-08 |
| GB1401896A (en) | 1975-08-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Frasch et al. | Competitive inhibition of phlorizin binding byd-glucose and the influence of sodium: A study on isolated brush border membrane of rat kidney | |
| Kean | Rapid, sensitive spectrophotometric method for quantitative determination of sulfatides | |
| DE69123519T2 (de) | Verfahren und Vorrichtung zur Bestimmung von Ionen | |
| DE2812870C2 (de) | Sauerstoffindikator | |
| DE69114403T2 (de) | Reagenz zur Proteinfällung. | |
| Potter et al. | Uptake of propranolol by isolated guinea-pig atria | |
| DE3872998T2 (de) | Test auf okkultes blut im stuhl. | |
| DE3024044C2 (https=) | ||
| Biernbaum et al. | Influence of light and calcium on guanosine 5'-triphosphate in isolated frog rod outer segments. | |
| Harris | The exchange of frog muscle potassium | |
| DE3851923T2 (de) | Reagenz zur Retikulozytenzählung mittels Durchflusszytometrie. | |
| DE3921609C2 (de) | Verfahren zur Bestimmung des Vorhandenseins, der Konzentration oder der Struktur eines Analyten | |
| Shorr | A New Technic for Staining Vaginal Smears: II | |
| Toll et al. | Energy utilization in the uptake of catecholamines by synaptic vesicles and adrenal chromaffin granules | |
| DE1959410B2 (de) | Indikator zur bestimmung der reduzierten pyridincoenzyme | |
| DE1673047C2 (de) | Laboratoriumsreagenz in trockener Form zur Bestimmung von alkalischer oder saurer Phosphatase | |
| DE1940816A1 (de) | Verfahren und diagnostisches Mittel zur enzymatischen Bestimmung von Glucose | |
| DE2339663A1 (de) | Testloesung zum nachweis der aktivitaet von succinodehydrogenase | |
| DE2921023C2 (de) | Zusammensetzung, Testmittel und Verfahren zum Bestimmen von Urobilinogen in einer Probe unter Verwendung der Zusammensetzung, sowie Verfahren zum Herstellen des Testmittels | |
| DE69525287T2 (de) | Endotoxin-Stabilisierungsmittel, Endotoxin-Zusammensetzung und Verfahren zur Bestimmung von Endotoxin | |
| DE2650213A1 (de) | Verfahren und mittel fuer den nachweis und/oder die bestimmung alkylierender verbindungen | |
| EP0358991B2 (de) | Testvorrichtung zur optischen Bestimmung von Kationen, Anionen oder elektrisch neutralen ionogenen Spezien und Testverfahren unter Verwendung dieser Testvorrichtung | |
| EP0825264B1 (de) | Stabile Mischung zum Nachweis alkalischer Phosphatase mit einem Salz der o-Kresolphthaleinmonophosphorsäure | |
| DE3779519T2 (de) | Analytisches element mit erhoehtem alkalischem phosphatase-isoenzym und verfahren zur bestimmung von theophyllin. | |
| DE2925365A1 (de) | Testeinrichtung zur bestimmung von harnsaeure in einer fluessigkeitsprobe und deren verwendung bei harnsaeurebestimmungen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |