DE2217801A1 - Verfahren zur Herstellung eines beständigen Absorbens aus Blutserum für die Diagnose der infektiösen Mononukleose - Google Patents
Verfahren zur Herstellung eines beständigen Absorbens aus Blutserum für die Diagnose der infektiösen MononukleoseInfo
- Publication number
- DE2217801A1 DE2217801A1 DE19722217801 DE2217801A DE2217801A1 DE 2217801 A1 DE2217801 A1 DE 2217801A1 DE 19722217801 DE19722217801 DE 19722217801 DE 2217801 A DE2217801 A DE 2217801A DE 2217801 A1 DE2217801 A1 DE 2217801A1
- Authority
- DE
- Germany
- Prior art keywords
- blood cells
- absorbent
- stroma
- diagnosis
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 19
- 210000002966 serum Anatomy 0.000 title claims description 19
- 201000006747 infectious mononucleosis Diseases 0.000 title claims description 16
- 239000002250 absorbent Substances 0.000 title claims description 12
- 230000002745 absorbent Effects 0.000 title claims description 12
- 238000003745 diagnosis Methods 0.000 title claims description 8
- 230000002085 persistent effect Effects 0.000 title claims description 3
- 210000003743 erythrocyte Anatomy 0.000 claims description 21
- 241001494479 Pecora Species 0.000 claims description 14
- 210000004369 blood Anatomy 0.000 claims description 9
- 239000008280 blood Substances 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 7
- 230000004520 agglutination Effects 0.000 claims description 6
- 210000000601 blood cell Anatomy 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000005348 Neuraminidase Human genes 0.000 claims description 4
- 108010006232 Neuraminidase Proteins 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003755 preservative agent Substances 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- QRLVDLBMBULFAL-UHFFFAOYSA-N Digitonin Natural products CC1CCC2(OC1)OC3C(O)C4C5CCC6CC(OC7OC(CO)C(OC8OC(CO)C(O)C(OC9OCC(O)C(O)C9OC%10OC(CO)C(O)C(OC%11OC(CO)C(O)C(O)C%11O)C%10O)C8O)C(O)C7O)C(O)CC6(C)C5CCC4(C)C3C2C QRLVDLBMBULFAL-UHFFFAOYSA-N 0.000 claims description 3
- 102000001554 Hemoglobins Human genes 0.000 claims description 3
- 108010054147 Hemoglobins Proteins 0.000 claims description 3
- 239000003146 anticoagulant agent Substances 0.000 claims description 3
- 229940127219 anticoagulant drug Drugs 0.000 claims description 3
- UVYVLBIGDKGWPX-KUAJCENISA-N digitonin Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)C[C@@H](O)[C@H](O[C@H]5[C@@H]([C@@H](O)[C@@H](O[C@H]6[C@@H]([C@@H](O[C@H]7[C@@H]([C@@H](O)[C@H](O)CO7)O)[C@H](O)[C@@H](CO)O6)O[C@H]6[C@@H]([C@@H](O[C@H]7[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O7)O)[C@@H](O)[C@@H](CO)O6)O)[C@@H](CO)O5)O)C[C@@H]4CC[C@H]3[C@@H]2[C@@H]1O)C)[C@@H]1C)[C@]11CC[C@@H](C)CO1 UVYVLBIGDKGWPX-KUAJCENISA-N 0.000 claims description 3
- UVYVLBIGDKGWPX-UHFFFAOYSA-N digitonine Natural products CC1C(C2(CCC3C4(C)CC(O)C(OC5C(C(O)C(OC6C(C(OC7C(C(O)C(O)CO7)O)C(O)C(CO)O6)OC6C(C(OC7C(C(O)C(O)C(CO)O7)O)C(O)C(CO)O6)O)C(CO)O5)O)CC4CCC3C2C2O)C)C2OC11CCC(C)CO1 UVYVLBIGDKGWPX-UHFFFAOYSA-N 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 230000002949 hemolytic effect Effects 0.000 claims 2
- 230000009102 absorption Effects 0.000 description 13
- 238000010521 absorption reaction Methods 0.000 description 13
- 239000000243 solution Substances 0.000 description 7
- 241000700199 Cavia porcellus Species 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 108090000526 Papain Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 229940055729 papain Drugs 0.000 description 4
- 235000019834 papain Nutrition 0.000 description 4
- 210000005084 renal tissue Anatomy 0.000 description 4
- 238000013207 serial dilution Methods 0.000 description 3
- 241000700198 Cavia Species 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/812—Infectious mononucleosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI711032A FI46893C (fi) | 1971-04-14 | 1971-04-14 | Menetelmä mononukleoositaudin toteamiseksi veriseerumista käyttämällä entsyymikäsiteltyjä verisolustroomia epäspesifien vasta-aineiden absor boimiseksi |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2217801A1 true DE2217801A1 (de) | 1972-10-19 |
Family
ID=8504994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19722217801 Pending DE2217801A1 (de) | 1971-04-14 | 1972-04-13 | Verfahren zur Herstellung eines beständigen Absorbens aus Blutserum für die Diagnose der infektiösen Mononukleose |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US3864467A (OSRAM) |
| DE (1) | DE2217801A1 (OSRAM) |
| FI (1) | FI46893C (OSRAM) |
| FR (1) | FR2133748B1 (OSRAM) |
| GB (1) | GB1364180A (OSRAM) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4139606A (en) * | 1976-05-24 | 1979-02-13 | Zichis Joseph | Preparation of antigen and test for heterophil antibody |
| US4460694A (en) * | 1981-03-26 | 1984-07-17 | University Of Miami | Bovine glycoproteins and use in diagnosing infectious mononucleosis |
-
1971
- 1971-04-14 FI FI711032A patent/FI46893C/fi active
-
1972
- 1972-04-12 US US243421A patent/US3864467A/en not_active Expired - Lifetime
- 1972-04-13 DE DE19722217801 patent/DE2217801A1/de active Pending
- 1972-04-14 GB GB1730572A patent/GB1364180A/en not_active Expired
- 1972-04-14 FR FR7213207A patent/FR2133748B1/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB1364180A (en) | 1974-08-21 |
| US3864467A (en) | 1975-02-04 |
| FR2133748A1 (OSRAM) | 1972-12-01 |
| FI46893C (fi) | 1973-07-10 |
| FR2133748B1 (OSRAM) | 1977-11-25 |
| FI46893B (OSRAM) | 1973-04-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69033033T2 (de) | Verbesserte kontrollverfahren für stabile blutkoagulation | |
| DE2322562C2 (de) | Verfahren zur Bestimmung von Antigenen in einer Probe | |
| Ditzel et al. | Changes in serum proteins, lipoproteins, and protein-bound carbohydrates in relation to pathologic alterations in the microcirculation of diabetic subjects | |
| DE68912115T2 (de) | Ausscheidungsverfahren für die herstellung von thromboplastinreagenzien. | |
| DE3205301C2 (OSRAM) | ||
| EP0780687A2 (de) | Verfahren zur Herstellung eines synthetischen Kalibrators für Sandwich-Immunoassays | |
| DE3105555C2 (OSRAM) | ||
| DE3609980A1 (de) | Verfahren zur bestimmung von haemoglobin im stuhl | |
| DE112006000047T5 (de) | Eine Indikatorzusammensetzung zum Bewerten der Reinigung eines medizinischen Instrumentes | |
| DE2850950C3 (de) | Verwendung von nativem oder thermisch modifiziertem Cytochrom c als Stabilisator für ein immunochemisches Meßreagens, immunochemisches Meßreagens sowie Pufferlösung zur Probenverdünnung bei immunchemischen Meßverfahren | |
| DE2217801A1 (de) | Verfahren zur Herstellung eines beständigen Absorbens aus Blutserum für die Diagnose der infektiösen Mononukleose | |
| DE2934756A1 (de) | Zusammensetzung zur bestimmung von beta 2 -humanmikroglobulin und verfahren zu deren herstellung und deren verwendung | |
| DE2907228C2 (de) | Immunochemische Bestimmung zur Bestimmung der LDH↓1↓-Aktivität | |
| CH636449A5 (de) | Verfahren zur gewinnung eines neuen, spezifischen alpha-l-antikoerpers. | |
| DE2054805A1 (de) | Indirekter Hamagglutmationstest bei gleichzeitiger Adsorption heterologer Antikörper | |
| DE2742835A1 (de) | Antigenfraktionen von schistosoma mansoni-eiern, geeignet fuer die untersuchung von schistosomiasis | |
| DE19621488C2 (de) | Diagnostisches Verfahren zur Erkennung subklinisch an Paratuberkulose erkrankter Säuger | |
| DE2023989B2 (de) | Verfahren zur Herstellung von aus Antigen Tragerstoffgemischen bestehenden und gegebenenfalls Antikörper enthalten den fertigen serodiagnostischen, auf einen Objektträger aufgebrachten lager bestandigen Testsubstanzen | |
| DE69132257T2 (de) | Verfahren zur Herstellung eines gereinigten Treponema-Antigens und dessen Verwendung | |
| EP0013306B1 (de) | Verfahren zur Bestimmung eines opsonierenden oberflächenbindenden alpha-2-Glykoproteins | |
| DE3872900T2 (de) | Verfahren zur bestimmung und zum zaehlen von teilchenfoermigen antigenen in einer fluessigen probe, insbesondere von clostridium butyricum-sporen in milch. | |
| DE3318761C2 (OSRAM) | ||
| DE69915437T2 (de) | Plasmasammlungen von mehr als 200 gesunden Patienten und davon abgeleiteter Kalibrierstandard | |
| DE2722077C3 (de) | Verfahren sowie wäßrige und lyophilisierte Zusammensetzungen zur Bestimmung des Endotoxingehaltes einer wäßrigen Flüssigkeit | |
| DD280176A5 (de) | Glutenenteropathietest |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHA | Expiration of time for request for examination |