DE19951189C2 - Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA - Google Patents
Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNAInfo
- Publication number
- DE19951189C2 DE19951189C2 DE19951189A DE19951189A DE19951189C2 DE 19951189 C2 DE19951189 C2 DE 19951189C2 DE 19951189 A DE19951189 A DE 19951189A DE 19951189 A DE19951189 A DE 19951189A DE 19951189 C2 DE19951189 C2 DE 19951189C2
- Authority
- DE
- Germany
- Prior art keywords
- cytosine
- sequence
- dna
- methylated
- oligomers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
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- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus For Radiation Diagnosis (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
- Control And Other Processes For Unpacking Of Materials (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Nonmetallic Welding Materials (AREA)
- Control Of Positive-Displacement Air Blowers (AREA)
- Stereophonic System (AREA)
- Paper (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
Priority Applications (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19951189A DE19951189C2 (de) | 1999-10-15 | 1999-10-15 | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
| US10/110,610 US7179594B1 (en) | 1999-10-15 | 2000-10-13 | Method for distinguishing 5-position methylation changes of cytosine bases and cytosine-to-thymine mutations and for detecting single nucleotide polymorphisms (SNPs) or point mutations in genomic DNA |
| NZ518318A NZ518318A (en) | 1999-10-15 | 2000-10-13 | Method for distinguishing 5-position methylation changes of cytosine bases and cytosine-to-thymine mutations |
| AU23451/01A AU775085B2 (en) | 1999-10-15 | 2000-10-13 | Method for distinguishing 5-position methylation changes |
| ES00987010T ES2246257T3 (es) | 1999-10-15 | 2000-10-13 | Procedimiento para la distincion de modificaciones de mutilacion en la posicion 5. |
| AT00987010T ATE299950T1 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
| DE50010776T DE50010776D1 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
| PCT/DE2000/003726 WO2001027317A2 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
| CA002387148A CA2387148A1 (en) | 1999-10-15 | 2000-10-13 | Method for distinguishing 5-position methylation changes |
| JP2001529448A JP4528475B2 (ja) | 1999-10-15 | 2000-10-13 | ５−位置メチル化変性体の識別方法 |
| IL14906700A IL149067A0 (en) | 1999-10-15 | 2000-10-13 | Method for distinguishing 5-position methylation changes |
| DK00987010T DK1228246T3 (da) | 1999-10-15 | 2000-10-13 | Fremgangsmåde til at skelne mellem methyleringsændringer i 5-stillingen |
| EP00987010A EP1228246B1 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
| IS6328A IS6328A (is) | 1999-10-15 | 2002-03-27 | Aðferð til að greina breytingar á metýleringu í 5. stöðu |
| US11/708,944 US20070161036A1 (en) | 1999-10-15 | 2007-02-20 | Method for distinguishing 5-position methylation changes of cytosine bases and cytosine-to-thymine mutations and for detecting single nucleotide polymorphisms (SNPs) or point mutations in genomic DNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19951189A DE19951189C2 (de) | 1999-10-15 | 1999-10-15 | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE19951189A1 DE19951189A1 (de) | 2001-05-03 |
| DE19951189C2 true DE19951189C2 (de) | 2003-11-06 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19951189A Expired - Fee Related DE19951189C2 (de) | 1999-10-15 | 1999-10-15 | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
| DE50010776T Expired - Lifetime DE50010776D1 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
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| DE50010776T Expired - Lifetime DE50010776D1 (de) | 1999-10-15 | 2000-10-13 | Verfahren zur unterscheidung von 5-position methylierungsänderungen |
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| US (2) | US7179594B1 (enExample) |
| EP (1) | EP1228246B1 (enExample) |
| JP (1) | JP4528475B2 (enExample) |
| AT (1) | ATE299950T1 (enExample) |
| AU (1) | AU775085B2 (enExample) |
| CA (1) | CA2387148A1 (enExample) |
| DE (2) | DE19951189C2 (enExample) |
| DK (1) | DK1228246T3 (enExample) |
| ES (1) | ES2246257T3 (enExample) |
| IL (1) | IL149067A0 (enExample) |
| IS (1) | IS6328A (enExample) |
| NZ (1) | NZ518318A (enExample) |
| WO (1) | WO2001027317A2 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19957827C2 (de) | 1999-11-25 | 2003-06-12 | Epigenomics Ag | Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche |
| DE10010282B4 (de) * | 2000-02-25 | 2006-11-16 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10151069A1 (de) * | 2001-10-05 | 2003-04-30 | Epigenomics Ag | Verfahren zum Nachweis von DNA-Methylierung mittels markierten S-Adenosylmethioninanaloga |
| DE10151055B4 (de) | 2001-10-05 | 2005-05-25 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln |
| US6916621B2 (en) | 2002-03-27 | 2005-07-12 | Spectral Genomics, Inc. | Methods for array-based comparitive binding assays |
| CA2442438C (en) | 2002-10-04 | 2012-06-19 | Nisshinbo Industries, Inc. | Oligonucleotide-immobilized substrate for detecting methylation |
| DE10338308B4 (de) * | 2003-08-15 | 2006-10-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA |
| US20100137154A1 (en) * | 2008-12-01 | 2010-06-03 | Ach Robert A | Genome analysis using a methyltransferase |
| DE102015009187B3 (de) | 2015-07-16 | 2016-10-13 | Dimo Dietrich | Verfahren zur Bestimmung einer Mutation in genomischer DNA, Verwendung des Verfahrens und Kit zur Durchführung des Verfahrens |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| WO1995021271A1 (en) * | 1994-02-07 | 1995-08-10 | Molecular Tool, Inc. | Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| CZ204597A3 (cs) * | 1997-06-26 | 1998-12-16 | I.N.T., Prof. Ing. Dr. Radko Krčma Drsc. | Způsob výroby objemné textilie s hladkým povrchem a zařízení k jeho provádění |
| AR017023A1 (es) * | 1997-08-29 | 2001-08-22 | Lopez Osvaldo J | Tipeo genomico de adn metiltransferasa |
| DE19935772C2 (de) * | 1999-07-26 | 2002-11-07 | Epigenomics Ag | Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben |
| MXPA03001834A (es) * | 2000-09-01 | 2003-08-01 | Epigenomics Ag | Metodo para determinar el grado de metilacion de determinadas citocinas en adn genomico en el contexto de secuencia 5'-cpg-3'. |
| DE10112515B4 (de) * | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| DE10201138B4 (de) * | 2002-01-08 | 2005-03-10 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA) |
-
1999
- 1999-10-15 DE DE19951189A patent/DE19951189C2/de not_active Expired - Fee Related
-
2000
- 2000-10-13 CA CA002387148A patent/CA2387148A1/en not_active Abandoned
- 2000-10-13 EP EP00987010A patent/EP1228246B1/de not_active Expired - Lifetime
- 2000-10-13 JP JP2001529448A patent/JP4528475B2/ja not_active Expired - Fee Related
- 2000-10-13 WO PCT/DE2000/003726 patent/WO2001027317A2/de not_active Ceased
- 2000-10-13 IL IL14906700A patent/IL149067A0/xx unknown
- 2000-10-13 US US10/110,610 patent/US7179594B1/en not_active Expired - Fee Related
- 2000-10-13 NZ NZ518318A patent/NZ518318A/en not_active IP Right Cessation
- 2000-10-13 DK DK00987010T patent/DK1228246T3/da active
- 2000-10-13 DE DE50010776T patent/DE50010776D1/de not_active Expired - Lifetime
- 2000-10-13 ES ES00987010T patent/ES2246257T3/es not_active Expired - Lifetime
- 2000-10-13 AT AT00987010T patent/ATE299950T1/de active
- 2000-10-13 AU AU23451/01A patent/AU775085B2/en not_active Ceased
-
2002
- 2002-03-27 IS IS6328A patent/IS6328A/is unknown
-
2007
- 2007-02-20 US US11/708,944 patent/US20070161036A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
Non-Patent Citations (4)
| Title |
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| Datenbank CAPLUS bei STN, AN 1996:85184 CAPLUS zu:Novel nonsense mutation in the hypoxanthine guanine phosphoribosyltransferase gene and nonradom X-inactivation causing Lesch-Nyhan syndrome in a female patient. Aral, B. et al., Hum. Mutat. (1996), 7(1), 52-8 * |
| Datenbank CAPLUS bei STN, AN 1997:40537 CAPLUS zu:A modified and improved method for bisulfide basedcytosine methylation analysis. Olek, A. et al., Nucleic Acids Res. (1996) 24(24), 5064-5066 * |
| Datenbank CAPLUS bei STN, AN 1998:734783 CAPLUS zu: Methylation levels at selected CpG sites in the factor VIII and FGFR3 genes, in mature fermaleand male germ cells: implications for male-driven evolution. El-Maarri, O. et al., Am. J.Hum. Genet.(1998), 63(4), 1001-1008 * |
| Datenbank MEDLINE bei STN, AN 96413032 MEDLINE zu:Cytosine methylation quantitation by automated genomic sequencing and GENESCAN analysis. Paul, C.L. & Clark, S.J., Biotechniques, (1996 Jul) * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1228246A2 (de) | 2002-08-07 |
| AU2345101A (en) | 2001-04-23 |
| IL149067A0 (en) | 2002-11-10 |
| EP1228246B1 (de) | 2005-07-20 |
| DE19951189A1 (de) | 2001-05-03 |
| WO2001027317A3 (de) | 2002-01-17 |
| ATE299950T1 (de) | 2005-08-15 |
| CA2387148A1 (en) | 2001-04-19 |
| AU775085B2 (en) | 2004-07-15 |
| IS6328A (is) | 2002-03-27 |
| JP2003511056A (ja) | 2003-03-25 |
| DK1228246T3 (da) | 2005-10-17 |
| ES2246257T3 (es) | 2006-02-16 |
| DE50010776D1 (de) | 2005-08-25 |
| US20070161036A1 (en) | 2007-07-12 |
| US7179594B1 (en) | 2007-02-20 |
| WO2001027317A2 (de) | 2001-04-19 |
| NZ518318A (en) | 2003-11-28 |
| JP4528475B2 (ja) | 2010-08-18 |
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