DE10207170A1 - Verfahren zur Grobklärung von Zellaufschlüssen aus Mikroorganismen - Google Patents
Verfahren zur Grobklärung von Zellaufschlüssen aus MikroorganismenInfo
- Publication number
- DE10207170A1 DE10207170A1 DE10207170A DE10207170A DE10207170A1 DE 10207170 A1 DE10207170 A1 DE 10207170A1 DE 10207170 A DE10207170 A DE 10207170A DE 10207170 A DE10207170 A DE 10207170A DE 10207170 A1 DE10207170 A1 DE 10207170A1
- Authority
- DE
- Germany
- Prior art keywords
- pressure
- microorganisms
- phase
- lysate
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 32
- 244000005700 microbiome Species 0.000 title claims abstract description 11
- 238000005352 clarification Methods 0.000 title claims abstract description 7
- 230000008569 process Effects 0.000 title description 9
- 239000013612 plasmid Substances 0.000 claims description 17
- 239000011541 reaction mixture Substances 0.000 claims description 8
- 239000012071 phase Substances 0.000 abstract description 18
- 239000006166 lysate Substances 0.000 abstract description 9
- 239000002244 precipitate Substances 0.000 abstract description 8
- 239000007791 liquid phase Substances 0.000 abstract description 4
- 102000039446 nucleic acids Human genes 0.000 abstract description 3
- 108020004707 nucleic acids Proteins 0.000 abstract description 3
- 150000007523 nucleic acids Chemical class 0.000 abstract description 3
- 239000013592 cell lysate Substances 0.000 abstract description 2
- 230000002934 lysing effect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract 1
- 239000000872 buffer Substances 0.000 description 17
- 230000009089 cytolysis Effects 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 8
- 238000001914 filtration Methods 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 5
- 239000002158 endotoxin Substances 0.000 description 5
- 238000006386 neutralization reaction Methods 0.000 description 5
- 210000002421 cell wall Anatomy 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000008004 cell lysis buffer Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005191 phase separation Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000011021 bench scale process Methods 0.000 description 2
- 210000003850 cellular structure Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- ONQDVAFWWYYXHM-UHFFFAOYSA-M potassium lauryl sulfate Chemical compound [K+].CCCCCCCCCCCCOS([O-])(=O)=O ONQDVAFWWYYXHM-UHFFFAOYSA-M 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 101000743092 Bacillus spizizenii (strain DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10) tRNA3(Ser)-specific nuclease WapA Proteins 0.000 description 1
- 101000743093 Bacillus subtilis subsp. natto (strain BEST195) tRNA(Glu)-specific nuclease WapA Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10207170A DE10207170A1 (de) | 2002-02-20 | 2002-02-20 | Verfahren zur Grobklärung von Zellaufschlüssen aus Mikroorganismen |
| JP2003569835A JP4344245B2 (ja) | 2002-02-20 | 2003-02-20 | 微生物由来溶解細胞物質の粗清澄化の方法 |
| DE50312615T DE50312615D1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
| AT03706546T ATE464380T1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
| PCT/EP2003/001769 WO2003070942A1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
| CA2474436A CA2474436C (en) | 2002-02-20 | 2003-02-20 | Process for the coarse clarification of lysed cell material from microorganisms |
| AU2003208740A AU2003208740B2 (en) | 2002-02-20 | 2003-02-20 | Method for coarse purification of cell digests from microorganisms |
| EP03706546A EP1478749B1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
| US10/504,258 US7214508B2 (en) | 2002-02-20 | 2003-02-20 | Method for coarse purification of cell digests from microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10207170A DE10207170A1 (de) | 2002-02-20 | 2002-02-20 | Verfahren zur Grobklärung von Zellaufschlüssen aus Mikroorganismen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10207170A1 true DE10207170A1 (de) | 2003-09-04 |
Family
ID=27674782
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10207170A Withdrawn DE10207170A1 (de) | 2002-02-20 | 2002-02-20 | Verfahren zur Grobklärung von Zellaufschlüssen aus Mikroorganismen |
| DE50312615T Expired - Lifetime DE50312615D1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE50312615T Expired - Lifetime DE50312615D1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7214508B2 (enExample) |
| EP (1) | EP1478749B1 (enExample) |
| JP (1) | JP4344245B2 (enExample) |
| AT (1) | ATE464380T1 (enExample) |
| CA (1) | CA2474436C (enExample) |
| DE (2) | DE10207170A1 (enExample) |
| WO (1) | WO2003070942A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2498518A1 (en) | 2002-09-13 | 2004-03-25 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
| EP2088196A1 (en) * | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Methods and devices for producing biomolecules |
| US11235323B2 (en) | 2008-08-27 | 2022-02-01 | Life Technologies Corporation | Apparatus for and method of processing biological samples |
| DK2331954T3 (da) | 2008-08-27 | 2020-04-06 | Life Technologies Corp | Apparat og fremgangsmåde til processering af biologiske prøver |
| GB2473868A (en) | 2009-09-28 | 2011-03-30 | Invitrogen Dynal As | Apparatus and method of automated processing of biological samples |
| US9724460B2 (en) | 2014-03-25 | 2017-08-08 | Oakwood Healthcare, Inc. | Controlled nucleation from gas-supersaturated liquid |
| JP2023508318A (ja) | 2019-12-18 | 2023-03-02 | ライフ テクノロジーズ コーポレーション | 自動核酸およびタンパク質単離のためのシステム、方法、およびデバイス |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4143639C2 (de) * | 1991-12-02 | 2002-10-24 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren |
| EP1023460A4 (en) | 1997-06-25 | 2003-09-10 | Promega Corp | METHOD FOR ISOLATING RNA |
| US6120985A (en) * | 1997-10-31 | 2000-09-19 | Bbi Bioseq, Inc. | Pressure-enhanced extraction and purification |
| CA2498518A1 (en) * | 2002-09-13 | 2004-03-25 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
-
2002
- 2002-02-20 DE DE10207170A patent/DE10207170A1/de not_active Withdrawn
-
2003
- 2003-02-20 WO PCT/EP2003/001769 patent/WO2003070942A1/de not_active Ceased
- 2003-02-20 AT AT03706546T patent/ATE464380T1/de active
- 2003-02-20 DE DE50312615T patent/DE50312615D1/de not_active Expired - Lifetime
- 2003-02-20 US US10/504,258 patent/US7214508B2/en not_active Expired - Lifetime
- 2003-02-20 JP JP2003569835A patent/JP4344245B2/ja not_active Expired - Fee Related
- 2003-02-20 EP EP03706546A patent/EP1478749B1/de not_active Expired - Lifetime
- 2003-02-20 CA CA2474436A patent/CA2474436C/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| CA 133:176702 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4344245B2 (ja) | 2009-10-14 |
| ATE464380T1 (de) | 2010-04-15 |
| DE50312615D1 (de) | 2010-05-27 |
| EP1478749A1 (de) | 2004-11-24 |
| CA2474436A1 (en) | 2003-08-28 |
| AU2003208740A1 (en) | 2003-09-09 |
| CA2474436C (en) | 2011-08-23 |
| US7214508B2 (en) | 2007-05-08 |
| EP1478749B1 (de) | 2010-04-14 |
| US20050089950A1 (en) | 2005-04-28 |
| WO2003070942A1 (de) | 2003-08-28 |
| JP2005517444A (ja) | 2005-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |
Effective date: 20110901 |