ATE464380T1 - Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen - Google Patents
Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismenInfo
- Publication number
- ATE464380T1 ATE464380T1 AT03706546T AT03706546T ATE464380T1 AT E464380 T1 ATE464380 T1 AT E464380T1 AT 03706546 T AT03706546 T AT 03706546T AT 03706546 T AT03706546 T AT 03706546T AT E464380 T1 ATE464380 T1 AT E464380T1
- Authority
- AT
- Austria
- Prior art keywords
- microorganisms
- lysate
- flocculent
- atmospheric pressure
- clarification
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 238000005352 clarification Methods 0.000 title abstract 2
- 239000006166 lysate Substances 0.000 abstract 3
- 239000012071 phase Substances 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 2
- 239000013592 cell lysate Substances 0.000 abstract 1
- 239000007791 liquid phase Substances 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10207170A DE10207170A1 (de) | 2002-02-20 | 2002-02-20 | Verfahren zur Grobklärung von Zellaufschlüssen aus Mikroorganismen |
| PCT/EP2003/001769 WO2003070942A1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE464380T1 true ATE464380T1 (de) | 2010-04-15 |
Family
ID=27674782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03706546T ATE464380T1 (de) | 2002-02-20 | 2003-02-20 | Verfahren zur grobklärung von zellaufschlüssen aus mikroorganismen |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7214508B2 (enExample) |
| EP (1) | EP1478749B1 (enExample) |
| JP (1) | JP4344245B2 (enExample) |
| AT (1) | ATE464380T1 (enExample) |
| CA (1) | CA2474436C (enExample) |
| DE (2) | DE10207170A1 (enExample) |
| WO (1) | WO2003070942A1 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2498518A1 (en) | 2002-09-13 | 2004-03-25 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
| EP2088196A1 (en) * | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Methods and devices for producing biomolecules |
| US11235323B2 (en) | 2008-08-27 | 2022-02-01 | Life Technologies Corporation | Apparatus for and method of processing biological samples |
| JP5492207B2 (ja) | 2008-08-27 | 2014-05-14 | ライフ テクノロジーズ コーポレーション | 生物学的サンプルの処理装置および処理方法 |
| GB2473868A (en) | 2009-09-28 | 2011-03-30 | Invitrogen Dynal As | Apparatus and method of automated processing of biological samples |
| US9724460B2 (en) | 2014-03-25 | 2017-08-08 | Oakwood Healthcare, Inc. | Controlled nucleation from gas-supersaturated liquid |
| WO2021126818A1 (en) | 2019-12-18 | 2021-06-24 | Life Technologies Corporation | Systems, methods, and devices for automated nucleic acid and protein isolation |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4143639C2 (de) * | 1991-12-02 | 2002-10-24 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren |
| JP2002507121A (ja) * | 1997-06-25 | 2002-03-05 | プロメガ コーポレイション | Ｒｎａの単離方法 |
| US6120985A (en) * | 1997-10-31 | 2000-09-19 | Bbi Bioseq, Inc. | Pressure-enhanced extraction and purification |
| CA2498518A1 (en) * | 2002-09-13 | 2004-03-25 | Valentis, Inc. | Apparatus and method for preparative scale purification of nucleic acids |
-
2002
- 2002-02-20 DE DE10207170A patent/DE10207170A1/de not_active Withdrawn
-
2003
- 2003-02-20 US US10/504,258 patent/US7214508B2/en not_active Expired - Lifetime
- 2003-02-20 EP EP03706546A patent/EP1478749B1/de not_active Expired - Lifetime
- 2003-02-20 AT AT03706546T patent/ATE464380T1/de active
- 2003-02-20 CA CA2474436A patent/CA2474436C/en not_active Expired - Fee Related
- 2003-02-20 JP JP2003569835A patent/JP4344245B2/ja not_active Expired - Fee Related
- 2003-02-20 WO PCT/EP2003/001769 patent/WO2003070942A1/de not_active Ceased
- 2003-02-20 DE DE50312615T patent/DE50312615D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7214508B2 (en) | 2007-05-08 |
| DE10207170A1 (de) | 2003-09-04 |
| EP1478749B1 (de) | 2010-04-14 |
| CA2474436A1 (en) | 2003-08-28 |
| DE50312615D1 (de) | 2010-05-27 |
| WO2003070942A1 (de) | 2003-08-28 |
| JP2005517444A (ja) | 2005-06-16 |
| JP4344245B2 (ja) | 2009-10-14 |
| EP1478749A1 (de) | 2004-11-24 |
| CA2474436C (en) | 2011-08-23 |
| AU2003208740A1 (en) | 2003-09-09 |
| US20050089950A1 (en) | 2005-04-28 |
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