DE102021134427A1 - Mikroskop und verfahren zur mikroskopie - Google Patents
Mikroskop und verfahren zur mikroskopie Download PDFInfo
- Publication number
- DE102021134427A1 DE102021134427A1 DE102021134427.1A DE102021134427A DE102021134427A1 DE 102021134427 A1 DE102021134427 A1 DE 102021134427A1 DE 102021134427 A DE102021134427 A DE 102021134427A DE 102021134427 A1 DE102021134427 A1 DE 102021134427A1
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- DE
- Germany
- Prior art keywords
- sample
- microscope
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- until
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000000386 microscopy Methods 0.000 title claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 103
- 230000005284 excitation Effects 0.000 claims abstract description 49
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 230000003287 optical effect Effects 0.000 claims description 15
- 238000009826 distribution Methods 0.000 claims description 13
- 210000001747 pupil Anatomy 0.000 claims description 9
- 238000005096 rolling process Methods 0.000 claims description 9
- 238000006073 displacement reaction Methods 0.000 claims description 8
- 230000001360 synchronised effect Effects 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 5
- 230000007935 neutral effect Effects 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 2
- 230000001427 coherent effect Effects 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 130
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- BUHVIAUBTBOHAG-FOYDDCNASA-N (2r,3r,4s,5r)-2-[6-[[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)ethyl]amino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound COC1=CC(OC)=CC(C(CNC=2C=3N=CN(C=3N=CN=2)[C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)C=2C(=CC=CC=2)C)=C1 BUHVIAUBTBOHAG-FOYDDCNASA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
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Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- General Engineering & Computer Science (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102021134427.1A DE102021134427A1 (de) | 2021-12-22 | 2021-12-22 | Mikroskop und verfahren zur mikroskopie |
| PCT/EP2022/082544 WO2023117236A1 (de) | 2021-12-22 | 2022-11-21 | Mikroskop und verfahren zur mikroskopie |
| CN202280084832.9A CN118451353A (zh) | 2021-12-22 | 2022-11-21 | 显微镜和用于显微镜检查的方法 |
| US18/723,002 US20250052991A1 (en) | 2021-12-22 | 2022-11-21 | Microscope and Method for Microscopy |
| JP2024538316A JP2025502743A (ja) | 2021-12-22 | 2022-11-21 | 顕微鏡及び顕微鏡法のための方法 |
| EP22818762.1A EP4453627B1 (de) | 2021-12-22 | 2022-11-21 | Mikroskop und verfahren zur mikroskopie |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102021134427.1A DE102021134427A1 (de) | 2021-12-22 | 2021-12-22 | Mikroskop und verfahren zur mikroskopie |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE102021134427A1 true DE102021134427A1 (de) | 2023-06-22 |
Family
ID=84439851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE102021134427.1A Pending DE102021134427A1 (de) | 2021-12-22 | 2021-12-22 | Mikroskop und verfahren zur mikroskopie |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20250052991A1 (https=) |
| EP (1) | EP4453627B1 (https=) |
| JP (1) | JP2025502743A (https=) |
| CN (1) | CN118451353A (https=) |
| DE (1) | DE102021134427A1 (https=) |
| WO (1) | WO2023117236A1 (https=) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102023005252A1 (de) * | 2023-12-19 | 2025-06-26 | Carl Zeiss Microscopy Gmbh | Mikroskop mit schneller quasikonfokaler Detektion |
| WO2025196227A1 (de) | 2024-03-20 | 2025-09-25 | Carl Zeiss Microscopy Gmbh | Superauflösendes mikroskop mit schneller quasikonfokaler detektion |
| DE102024118478A1 (de) * | 2024-06-29 | 2025-12-31 | Carl Zeiss Microscopy Gmbh | Mikroskop, slidereader und verfahren zur mikroskopie |
| DE102024118477A1 (de) * | 2024-06-29 | 2025-12-31 | Carl Zeiss Microscopy Gmbh | Mikroskop, slidereader und verfahren zur mikroskopie |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7335898B2 (en) | 2004-07-23 | 2008-02-26 | Ge Healthcare Niagara Inc. | Method and apparatus for fluorescent confocal microscopy |
| GB2442576B (en) | 2006-10-05 | 2010-04-28 | Cairn Res Ltd | Optical imaging device |
| DE102009060793A1 (de) | 2009-12-22 | 2011-07-28 | Carl Zeiss Microlmaging GmbH, 07745 | Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten |
| WO2013106731A1 (en) | 2012-01-11 | 2013-07-18 | Howard Hughes Medical Institute | Multi-dimensional imaging using multi-focus microscopy |
| US10802256B2 (en) | 2014-02-12 | 2020-10-13 | Carl Zeiss Microscopy Gmbh | Multifocal scanning fluorescence microscope |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012002893A1 (en) * | 2010-06-30 | 2012-01-05 | Ge Healthcare Bio-Sciences Corp | A system for synchronization in a line scanning imaging microscope |
| US11231570B2 (en) * | 2018-04-30 | 2022-01-25 | University Of Central Florida Research Foundation, Inc. | Highly inclined swept tile (HIST) imaging apparatus, methods, and applications |
| EP3614190A1 (en) * | 2018-08-20 | 2020-02-26 | Till GmbH | Microscope device with virtual objective |
-
2021
- 2021-12-22 DE DE102021134427.1A patent/DE102021134427A1/de active Pending
-
2022
- 2022-11-21 WO PCT/EP2022/082544 patent/WO2023117236A1/de not_active Ceased
- 2022-11-21 US US18/723,002 patent/US20250052991A1/en active Pending
- 2022-11-21 JP JP2024538316A patent/JP2025502743A/ja active Pending
- 2022-11-21 EP EP22818762.1A patent/EP4453627B1/de active Active
- 2022-11-21 CN CN202280084832.9A patent/CN118451353A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7335898B2 (en) | 2004-07-23 | 2008-02-26 | Ge Healthcare Niagara Inc. | Method and apparatus for fluorescent confocal microscopy |
| GB2442576B (en) | 2006-10-05 | 2010-04-28 | Cairn Res Ltd | Optical imaging device |
| DE102009060793A1 (de) | 2009-12-22 | 2011-07-28 | Carl Zeiss Microlmaging GmbH, 07745 | Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten |
| WO2013106731A1 (en) | 2012-01-11 | 2013-07-18 | Howard Hughes Medical Institute | Multi-dimensional imaging using multi-focus microscopy |
| US10802256B2 (en) | 2014-02-12 | 2020-10-13 | Carl Zeiss Microscopy Gmbh | Multifocal scanning fluorescence microscope |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102023005252A1 (de) * | 2023-12-19 | 2025-06-26 | Carl Zeiss Microscopy Gmbh | Mikroskop mit schneller quasikonfokaler Detektion |
| WO2025196227A1 (de) | 2024-03-20 | 2025-09-25 | Carl Zeiss Microscopy Gmbh | Superauflösendes mikroskop mit schneller quasikonfokaler detektion |
| DE102024118478A1 (de) * | 2024-06-29 | 2025-12-31 | Carl Zeiss Microscopy Gmbh | Mikroskop, slidereader und verfahren zur mikroskopie |
| DE102024118477A1 (de) * | 2024-06-29 | 2025-12-31 | Carl Zeiss Microscopy Gmbh | Mikroskop, slidereader und verfahren zur mikroskopie |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2025502743A (ja) | 2025-01-28 |
| WO2023117236A1 (de) | 2023-06-29 |
| CN118451353A (zh) | 2024-08-06 |
| EP4453627A1 (de) | 2024-10-30 |
| US20250052991A1 (en) | 2025-02-13 |
| EP4453627B1 (de) | 2026-02-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R163 | Identified publications notified |